• Title/Summary/Keyword: Strain-based

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Taxonomy of Botryotrichum luteum sp. nov. based on Morphology and Phylogeny Isolated from Soil in Korea

  • Jung-Joo Ryu;Kallol Das;Seong-Keun Lim;Soo-Min Hong;Seung-Yeol Lee;Hee-Young Jung
    • Mycobiology
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    • v.51 no.2
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    • pp.72-78
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    • 2023
  • In this study, a fungal strain KNUF-22-025 belonging to the genus Botryotrichum was isolated from the soil in Korea. The cultural and morphological characteristics of this strain differed from those of closely related species. On malt extract agar, strain KNUF-22-025 showed slower growth than most of the related species, except B. domesticum. The conidia size (9.6-21.1×9.9-18.4 ㎛) of strain KNUF-22-025 was larger than those of B. piluliferum, B. domesticum, and B. peruvianum but smaller than those of B. atrogriseum and B. iranicum. Conidiophores in strain KNUF-22-025 (137 ㎛) were longer than those in other closely related species but shorter than those in B. atrogriseum. Multi-locus analysis of molecular markers, such as ITS, 28S ribosomal DNA, RBP2, and TUB2 revealed that strain KNUF-22-025 was distinct from other Botryotrichum species. Thus, this strain is proposed as a novel species based on morphological characteristics along with molecular phylogeny and named Botryotrichum luteum sp. nov.

Numerical Formulation of Consolidation Based on Finite Strain Analysis (대변형 압밀방정식의 수식화)

  • Shin, Ho-Sung;Lee, Seung-Rae
    • Journal of the Korean Geotechnical Society
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    • v.29 no.6
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    • pp.77-86
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    • 2013
  • Embankments on soft ground experience significant deformation during time-dependent consolidation settlement, as well as an initial undrained settlement. Since infinitesimal strain theory assumes no configuration change and minute strain during deformation, finite strain analysis is required for better prediction of geotechnical problems involving large strain and geometric change induced by imposed loadings. Updated Lagrangian formulation is developed for time-dependent consolidation combining both force equilibrium and mass conservation of fluid, and mechanical constitutive equation is written in Janumann stress rate. Numerical convergence during Newton's iteration in large deformation analysis is improved by Nagtegaal's approach of considering the effect of rotation in mechanical constitutive relationship. Numerical simulations are conducted to discuss numerical reliability and applicability of developed numerical code: deformation of cantilever beam, two-dimensional consolidation. The numerical results show that developed formulation can efficiently describe large deformation problems. Proposed formulation is expected to facilitate the upgrading of a numerical code based on infinitesimal strain theory to that based on finite strain analysis.

A Viscoplastic Constitutive Model Based on Overstress Concept with Time-Temperature Superposition Principle (시간-온도 중첩이론을 적용한 아스팔트 바인더의 점소성 구성 모형)

  • Yun, Tae-Young;Ohm, Byung-Sik;Yoo, Pyeong-Jun;Kim, Yeon-Bok
    • International Journal of Highway Engineering
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    • v.14 no.5
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    • pp.75-83
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    • 2012
  • PURPOSES: Suggestion of asphalt binder constitutive model based on time-temperature superposition principle and overstress concept in order to describe behavior of asphalt binders. METHODS: A series of temperature sweep tests and multiple stress creep and recovery(MSCR) tests are performed to verify the applicability of time-temperature superposition principle(t-Ts) and to develop viscoelastoplastic constitutive equation based on overstress concept. For the tests, temperature sweep tests at various high temperature and various frequency and MSCR test at $58^{\circ}C$, $64^{\circ}C$ $70^{\circ}C$, $76^{\circ}C$, and $82^{\circ}C$ are performed. From the temperature sweep tests, dynamic shear modulus mastercurve and time-temperature shift function are built and the shift function and MSCR at $58^{\circ}C$ are utilized to determine model coefficients of VBO model. RESULTS: It is observed that the time-temperature shift function built at low strain level of 0.1% is applicable not only to 1.0% strain level temperature sweep test but also maximum 500,00% strain level of MSCR test. As well, the modified VBO model shows perfect prediction on MSCR measured strain at the other temperatures. CONCLUSIONS: The Time-temperature superposition principle stands hold from very low strain level to very high strain level and that the modified VBO model can be applicable for various range of strain and temperature region to predict elastic, viscoelastic, and viscoplastic strain of asphalt binders.

Development of strain-specific polymerase chain reaction primers to detect Fusobacterium hwasookii strains

  • Lim, Yun Kyong;Kook, Joong-Ki
    • International Journal of Oral Biology
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    • v.46 no.4
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    • pp.155-159
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    • 2021
  • This study aimed to develop strain-specific polymerase chain reaction (PCR) primers to detect Fusobacterium hwasookii KCOM 1249T, F. hwasookii KCOM 1253, F. hwasookii KCOM 1256, F. hwasookii KCOM 1258, and F. hwasookii KCOM 1268 on the basis of nucleotide sequences of a gene specific to each strain. The unique genes for each F. hwasookii strain were determined on the basis of their genome sequences using Roary. The strain-specific PCR primers based on each strain-specific gene were designed using PrimerSelect. The specificity of each PCR primer was determined using the genomic DNA of the 5 F. hwasookii strains and 25 strains of oral bacterial species. The detection limit and sensitivity of each strain-specific PCR primer pair were determined using the genomic DNA of each target strain. The results showed that the strain-specific PCR primers correspond to F. hwasookii KCOM 1249T, F. hwasookii KCOM 1253, F. hwasookii KCOM 1258, F. hwasookii KCOM 1256/F. nucleatum subsp. polymorphum KCOM 1260, or F. hwasookii KCOM 1268/Fusobacterium sp. oral taxon 203 were developed. The detection limits of these strain-specific PCR primers ranged from 0.2 to 2 ng of genomic DNA for each target strain. The results suggest that these strain-specific PCR primers are valuable in quality control for detecting specific F. hwasookii strains.

Patch-type large strain sensor using elastomeric composite filled with carbon nanofibers

  • Yasuoka, Tetsuo;Shimamura, Yoshinobu;Todoroki, Akira
    • International Journal of Aeronautical and Space Sciences
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    • v.14 no.2
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    • pp.146-151
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    • 2013
  • Carbon nanofibers (CNFs) are electrically conductive. When CNFs are used as fillers in resin, this electrical conductivity can be yielded without adversely affecting the mechanical properties of the resin. When an elastomer is adopted as the resin, a conductive elastomer can then be produced. Due to its flexibility and conductive properties, a large strain sensor based on changes in resistivity may be produced, for strain sensing in flexible structures. In this study, a patch-type large strain sensor using resistivity change in a CNF/elastomer composite was proposed. The measurement limits of the sensor were investigated experimentally, and the limit was found to be 40%, which greatly exceeded the limits of conventional metal-foiled strain gages. Also, the proposed CNF/elastomer large strain sensor can be used to measure flexible materials, while conventional strain gages cannot be used to measure such strains.

Stress-Strain Properties of Geosynthetics by Confined Extension Tests (구속신장시험에 의한 토목섬유의 인장력-변형률거동 특성)

  • Bang, Yoon-Kyung;Jeon, Young-Dae;Lee, Jun-Dae
    • Journal of the Korean Society of Safety
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    • v.17 no.2
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    • pp.52-57
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    • 2002
  • In this study. stress-strain relationships were investigated by performing the confined extension tests for seven types of geosynthetics such as geotextiles, composite geosynthetics and geogrids. A comparison was made between unconfined and confined moduli for each geosynthetic material to quantify the soil confinement effect on stress-strain properties. A comparison was also made between the increase of moduli at the same strain level with the types of the geosynthetics to demonstrate the different stress-strain responses. Based on the result of the extension tests, the higher the confining stress, the larger the secant modulus of geosynthetics. The secant modulus at 5% strain is twice as much as that of 10% strain, especially there is a noticeable increasing of secant modulus for the two nonwoven geotextiles.

Rate-sensitive analysis of framed structures part II: implementation and application to steel and R/C frames

  • Fang, Q.;Izzuddin, B.A.
    • Structural Engineering and Mechanics
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    • v.5 no.3
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    • pp.239-256
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    • 1997
  • The companion paper presents a new three-parameter model for the uniaxial rate-sensitive material response, which is based on a bilinear static stress-strain relationship with kinematic strain-hardening. This paper extends the proposed model to trilinear static stress-strain relationships for steel and concrete, and discusses the implementation of the new models within an incremental-iterative solution procedure. For steel, the three-parameter rate-function is employed with a trilinear static stress-strain relationship, which allows the utilisation of different levels of rate-sensitivity for the plastic plateau and strain-hardening ranges. For concrete, on the other hand, two trilinear stress-strain relationships are used for tension and compression, where rate-sensitivity is accounted for in the strain-softening range. Both models have been implemented within the nonlinear analysis program ADAPTIC, which is used herein to provide verification for the models, and to demonstrate their applicability to the rate-sensitive analysis of steel and reinforced concrete structures.

Tensile strength of unidirectional CFRP laminate under high strain rate

  • Taniguchi, Norihiko;Nishiwaki, Tsuyoshi;Kawada, Hiroyuki
    • Advanced Composite Materials
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    • v.16 no.2
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    • pp.167-180
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    • 2007
  • The tensile strength of unidirectional carbon fiber reinforced plastics under a high strain rate was experimentally investigated. A high-strain-rate test was performed using the tension-type split Hopkinson bar technique. In order to obtain the tensile stress-strain relations, a special fixture was used for the impact tensile specimen. The experimental results demonstrated that the tensile modulus and strength in the longitudinal direction are independent of the strain rate. In contrast, the tensile properties in the transverse direction and the shear properties increase with the strain rate. Moreover, it was observed that the strain-rate dependence of the shear strength is much stronger than that of the transverse strength. The tensile strength of off-axis specimens was measured using an oblique tab, and the experimental results were compared with the tensile strength predicted based on the Tsai-Hill failure criterion. It was concluded that the tensile strength can be characterized quite well using the above failure criterion under dynamic loading conditions.

An asymptotic multi-scale approach for beams via strain gradient elasticity: surface effects

  • Kim, Jun-Sik
    • Multiscale and Multiphysics Mechanics
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    • v.1 no.1
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    • pp.15-33
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    • 2016
  • In this paper, an asymptotic method is employed to formulate nano- or micro-beams based on strain gradient elasticity. Although a basic theory for the strain gradient elasticity has been well established in literature, a systematic approach is relatively rare because of its complexity and ambiguity of higher-order elasticity coefficients. In order to systematically identify the strain gradient effect, an asymptotic approach is adopted by introducing the small parameter which represents the beam geometric slenderness and/or the internal atomistic characteristic. The approach allows us to systematically split the two-dimensional strain gradient elasticity into the microscopic one-dimensional through-the-thickness analysis and the macroscopic one-dimensional beam analysis. The first-order beam problem turns out to be different from the classical elasticity in terms of the bending stiffness, which comes from the through-the-thickness strain gradient effect. This subsequently affects the second-order transverse shear stress in which the surface shear stress exists. It is demonstrated that a careful derivation of a first strain gradient elasticity embraces "Gurtin-Murdoch traction" as the surface effect of a one-dimensional Euler-Bernoulli-like beam model.

Development of Optical Strain Sensor with Nanostructures on a Poly-dimethylsiloxane (PDMS) Substrate (Poly-dimethylsiloxane (PDMS) 기판 위에 형성된 나노구조를 이용한 시각 인장센서의 개발)

  • Kim, Geon Hwee;Woo, Hyeonsu;Lim, Geunbae;An, Taechang
    • Journal of Sensor Science and Technology
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    • v.27 no.6
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    • pp.392-396
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    • 2018
  • Structural color has many advantages over pigment based color. In recent years, researches are being conducted to apply these advantages to applications such as wearable devices. In this study, strain sensor, a kind of wearable device, was developed using structural color. The use of structural color has the advantage of not using energy and complex measuring equipment to measure strain rate. Wrinkle structure was fabricated on the surface of Poly-dimethylsiloxane (PDMS) and used it as a sensor which color changes according to the applied strain. In addition, a transmittance-changing sensor was developed and fabricated by synthesizing additional glass nanoparticles. Furthermore, a strain sensor was developed that is largely transparent at the target strain and opaque otherwise.