• Title/Summary/Keyword: Strain level

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Design of Metal Cored Wire for Erosion Resistant Overlay Welding

  • Kim, Jun-Ki;Kim, In-Ju;Kim, Ki-Nam;Kim, Ji-Hui;Kim, Seon-Jin
    • Proceedings of the KWS Conference
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    • 2009.11a
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    • pp.202-204
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    • 2009
  • Erosion is a common failure mode of materials frequently encountered in plant and power industry. Although the erosion resistance of Fe-base alloy has been inferior to the other expensive materials, it is expected that the strain-induced martensitic transformation can impart high erosion resistance to Fe-base alloy. The key technology to develop Fe-base metal cored welding wire for erosion resistant overlay welding may include the strain-induced metallurgy for hardening rate control and the welding flux metallurgy for dilution control. Sophisticated studies showed that the strain-induced martensitic transformation behavior was related to the critical strain energy which was dependent on the alloy composition. Dilution and bead shape of overlay weld were proved to be affected by metal transfer mode during gas tungsten arc welding and elements in welding fluxes. It was considered that the highly erosion resistant Fe-base overlay weld could be achieved by precise control of alloy composition to have proper level of critical strain energy for energy absorption and welding flux formulation to have small amount of deoxidizing metallic elements for dilution.

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Comparison between of the Attenuated BR-Oka and the Wild Type Strain of Varicella Zoster Virus (VZV) on the DNA level

  • Lim, Sang-Min;Song, Seong-Won;Kim, Sang-Lin;Jang, Yoon-Jung;Kim, Ki-Ho;Kim, Hong-Jin
    • Archives of Pharmacal Research
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    • v.23 no.4
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    • pp.418-423
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    • 2000
  • Oka strain VR-795 (Varicella Zoster Virus, VZV) of American Type Culture Collection (ATCC) has been used for chickenpox vaccine production. In order to use this strain for vaccine production, the strain must be identified and its stability must be confirmed. The identification of the Oka strain has been confirmed using Restriction Fragment Length Polymorphism (RFLP) and DNA sequence analysis of glycoprotein-II (gp-II). The amino acid sequences of Oka deduced from the DNA sequence of gp-II have changed at three amino acids against Ellen and at one amino acid against Webster. To prove the stability of the Oka strain during the passage, RFLP and DNA sequence analyses were also used with 11, 15 and 23 times of virus passage. We found that the Oka strain was stable at passages of up to 23 times, based on the RFLP and DNA sequence analyses. The confirmed Oka strain was renamed as BR-Oka for the purposes of chickenpox vaccine production.

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Genetic Stock Identification of Common Carp (Cyprinus carpio) by Detection of Intraspecific DNA Sequence Variation in the Mitochondrial 12S rRNA Gene (미토콘드리아 12S rRNA 유전자 변이 조사를 통한 잉어(Cyprinus carpio)의 유전학적 동정)

  • 남윤권;주수동;정창화;노충환;조재윤;김동수
    • Journal of Aquaculture
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    • v.10 no.4
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    • pp.403-407
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    • 1997
  • Intraspecific sequence variation was detected by polymerase chain reaction (PCR) and direct sequencing of a 350-nucleotide region of the mitochondrial 12S rRNA gene of two natural populations (Han River and Nakdong River) and one hatchery stock (Jinhae Inland Fisheries Institute) of local strain common carp, one Israeli strain of common carp stock from Pukyong National University (PKU), and one hybrid between Israeli strain of common carp female and local strain common carp male from PKU stock. There is little variation in 350 bases of the mitochondrial 12S rRNA gene sequences among 2 natural and 1 hatchery local strain common carp populatins, representing abut 7 to 20 nucleotide differences (less than 6%). The sequence of specimens from Han River was more similar to that from Nakdong River (identity=98.0%) than to that from Jinhae Inland Fisheries Institute (identity=96.3%). Sequence variation between Israeli strain and wild local strain common carp was higher than the variation within natural stocks. The level of variation was ranged from 15.7 to 17.7%. The hybrid showed very similar nucleotide4 sequence of 12S rRNA gene to the sequence of Israeli strain with the identity of 98.9%.

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Plasticity and Fracture Behaviors of Marine Structural Steel, Part V: Effects of Strain Rate and Temperature (조선 해양 구조물용 강재의 소성 및 파단 특성 V: 온도 의존성을 고려한 변형률 속도에 관한 실험적 연구)

  • Choung, Joon-Mo;Im, Sung-Woo;Kim, Kyung-Su
    • Journal of Ocean Engineering and Technology
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    • v.25 no.3
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    • pp.73-84
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    • 2011
  • This is the fifth in a series of companion papers dealing with the dynamic hardening properties of various marine structural steels at intermediate strain rates. Five steps of strain rate levels (0.001, 1, 10, 100, 200/s) and three steps of temperature levels (LT ($-40^{\circ}C$), RT, and HT ($200^{\circ}C$)) were taken into account for the dynamic tensile tests of three types of marine structural steels: API 2W50 and Classifications EH36 and DH36. The total number of specimens was 180 pieces. It was seen that the effects of dynamic hardening became clearer at LT than at RT. Dynamic strain aging accompanying serrated flow stress curves was also observed from high temperature tests for all kinds of steels. The dynamic hardening factors (DHFs) at the two temperature levels of LT and RT were derived at the three plastic strain levels of 0.05, 0.10, 0.15 from dynamic tensile tests. Meanwhile, no DHFs were found for the high temperature tests because a slight negative strain rate dependency due to dynamic strain aging had occurred. A new formulation to determine material constant D in a Cowper-Symonds constitutive equation is provided as a function of the plastic strain rate, as well as the plastic strain level. The proposed formula is verified by comparing with test flow stress curves, not only at intermediate strain rate ranges but also at high strain rate ranges.

Actinoplanes teichomyceticus의 변이주에 의한 Teicoplanin 발효생산

  • Kim, Seong-Geon;An, Hyeon-Jin;Kim, Jae-Yeong;No, Yong-Taek
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.299-302
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    • 2000
  • Actinoplanes teichomyceticus ATCC 31121 was mutated with UV to obtain a superior mutant strain with increasing level of teicoplanin production. In this investigation lethal curve was obtained and the optimal condition to induce mutagenesis was determined and to isolate the desirable mutant strain. It was also confirmed that teicoplanin activities by agar diffusion method to be compared to the mother strain. One mutant strain, T991014-1 that had the highest teicoplanin productivity was finally selected for further investigation including fermentation pattern. The mutant was characterized by the various tests such as amylase activity, protease activity, antibiotic resistance, autotoxicity, and productivity.

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A Material Simulation of High-Strain-Rate Deformation with Dislocations and Vacancies (전위 및 공공을 고려한 고변형률 변형에 대한 재료 시뮬레이션)

  • Choi, Deok-Kee;Ryu, Han-Kyu
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.28 no.9
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    • pp.1306-1313
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    • 2004
  • This paper addresses a theoretical approach to calculate the amount of the stored energy during high strain-rate deformations using atomistic level simulation. The dynamic behavior of materials at high strain-rate deformation are of great interest. At high strain-rates deformations, materials generate heat due to plastic work and the temperature rise can be significant, affecting various properties of the material. It is well known that a small percent of the energy input is stored in the material, and most of input energy is converted into heat. However, microscopic analysis has not been completed without construction of a material model, which can simulate the movement of dislocations and vacancies. A major cause of the temperature rise within materials is traditionally credited to dislocations, vacancies and other defects. In this study, an atomistic material model for FCC such as copper is used to calculate the stored energy.

Strain Behavior of Ultra-high-strength Concrete under High Temperature and Loading (고온 및 재하에 따른 초고강도콘크리트의 변형거동)

  • Kim, Gyu-Yong;Nam, Jeong-Soo;Choe, Gyeong-Cheol;Yoon, Min-Ho;Hwang, Eui-Chul;Baek, Jae-Uk
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2017.05a
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    • pp.86-87
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    • 2017
  • The high-temperature creep of Ultra-High-Strength Concrete (UHSC) has been investigated in this study. The purpose of this study is to evaluated total strain and high-temperature creep at elevated temperatures under loading condition of UHSC. As results, Total strain of UHSC increased showing shrinkage with increasing compressive strength. The high-temperature creep of UHSC increased with the temperature and higher level of compressive strength showed bigger high-temperature creep.

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Numerical Identification of Streptomyces fIaveus Producing Antibiotic Substances Inhibitory to Plant Pathogenic Fungi

  • Lee, Jung-Yeop;Kim, Beom-Seok;Hwang, Byung-Kook
    • Journal of Microbiology and Biotechnology
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    • v.5 no.6
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    • pp.324-334
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    • 1995
  • The actinomycete strain A 11 was antagonistic to plant pathogenic fungi Phytophthora capsid and Magnaporthe grisea. Based on the diaminopimelic acid (DAP) type and morphological characteristics examined by scanning electron microscopy, the strain A 11 was confirmed to belong to the genus Streptomyces. Based on Willcox probability and similarity level, the strain A 11 was numerically identified as Streptomyces flaveus using TAXON program of Ward and Goodfellow. Antibiotic production of S. flaveus strain A 11 was most favorable when cultured on glycerol yeast extract peptone (GYP) agar for 20 days at $28^{\circ}C$. The crude antibiotics from solid GYP agar cultures of the strain A 11 were most effective against Phytophthora capsici and Sclerotinia sclerotiorum among the fungi tested. Antifungal activity of the antibiotics against Alternaria solani, Botryosphaeria dothidea, Cercospora capsici, Magnaporthe grisea, and Rhizoctonia solani was somewhat high, whereas Colletotrichum gloeosporioides and Fusarium oxysporum f. sp. cucumerinum were rarely inhibited even at high concentrations.

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Isolation and Identification of Pseudomonas sp. CMC-50 Producing Carboxymethyl Cellulase and Characterization of Its Crude Enzyme

  • Kim, Hyun-Joong;Kim, Hae-Yeong
    • Journal of Applied Biological Chemistry
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    • v.43 no.2
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    • pp.74-78
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    • 2000
  • A strain that produces a high level of carboxymethyl cellulase was isolated from rotten leaves. The isolated strain was revealed to be gram-negative, oxidase-positive, and catalase-negative. From the electron microscopic features, it was identified as a rod-shaped bacterium with peritrichous flagella and did not form spores. Morphological and biochemical characteristics of the strain were found to be similar to the Pseudomonas species. However, carbon utilization test showed different results. Based on the results, this new strain was identified as Pseudomonas sp. CMC-50. CMCase produced by this strain showed a strong activity in neutral and weak acidic conditions and maximum activity at $50^{\circ}C$.

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Enhancing hydrogen evolution activity of MoS2 basal plane by substitutional doping and strain engineering

  • Kim, Byeong-Hun;Lee, Byeong-Ju
    • Proceeding of EDISON Challenge
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    • 2016.03a
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    • pp.280-284
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    • 2016
  • 본 연구에서는 Density functional theory(DFT) 계산을 이용하여, $MoS_2$의 Mo와 S를 다른 원자로 치환 했을 때 $2H-MoS_2$ monolayer의 basal plane에서 HER활성을 향상시켰다. 특히 Ge와 Rh를 치환한 경우, ${\Delta}G_H$가 각각 0.03eV, 0,07eV로 최적에 가까운 HER활성이 나타났다. 다른 원자의 치환이 Fermi level 근처의 DOS(density of states)를 높여, ${\Delta}G_H$을 0에 가깝게 낮출 수 있음을 확인하였다. 또한 치환되는 원자의 농도, 그리고 strain을 변화시켜 농도와 strain의 증가에 따른 ${\Delta}G_H$ 감소를 발견했다. 이로써 각치환되는 원자마다, 치환 농도와 strain을 함께 변화시켜 ${\Delta}G_H$을 낮출 수 있었다. ${\Delta}G_H$가 0에 가까운(${\pm}{\pm}0.2eV$ 이내) 원자종류, 치환 농도, strain의 여러 조합을 찾았다.

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