• Title/Summary/Keyword: Strain Type

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The Characteristics of the Set-up Effect of Driven Piles (타입 말뚝의 지지력 증가효과 특성)

  • 조천환
    • Journal of the Korean Geotechnical Society
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    • v.19 no.4
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    • pp.235-246
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    • 2003
  • Since the study of Lee et al.(1994) there have been some case studies on the set-up effect of driven piles in Korea country. However, comprehensive examination on the analyses of the set-up effect with various testing data has not been carried out. In particular, the analysis of the influence of soil type and pile shape on the set-up effect has not been reported. It is necessary to analyse the test results of production piles in order to apply the set-up effect of driven piles for the field engineering. In this study some test piling and analyses were performed to give basic information to the piling design as well as the research on the set-up effect in sandy soils. The analyses on the set-up effect were performed with the monitoring data obtained from the high-strain dynamic loading tests. It was shown that the set-up effect of driven piles was not only affected by soil type but also by soil formation history It turned out that the set-up effect in sandy soils was considerable one that should not be ignored in the field, and that the bearing capacity increase of pile is mainly caused by the increase of shaft resistance. It was shown that the set-up effect of closed pile was larger than that of opened pile in clayey soils, while the set-up effect of opened pile was larger than that of closed pile in sandy soils.

Fatigue Evaluation of Precast Concrete Deck Connection using Ultra-High Performance, Fiber Reinforced Concrete (초고성능 섬유보강 콘크리트를 적용한 프리캐스트 바닥판 접합부의 피로성능 평가)

  • Lee, Jun-Ki
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.35 no.2
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    • pp.275-285
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    • 2015
  • This experimental study presents the fatigue evaluation of a precast deck connected using Ultra-High Performance, Fiber Reinforced Concrete (UHPFRC). Four types of two identical large-scale specimens were fabricated with simplified splice rebar details which had a short splice length of ten times rebar diameter. The flexural behavior of each type of specimens until failure was investigated and fatigue behavior of the same type of specimens was then evaluated using two-million cyclic loading. In the flexural tests, tensile rebars exhibited the deformation exceeding yielding strain but failure mode related to the splice details was not observed in spite of such a short splice length. In the fatigue tests, damage was not appreciably accumulated by the cyclic loading except initial flexural cracks and the stress variations in tensile rebars was less than the allowable stress range. These experimental results demonstrate that all types of specimens exhibited acceptable fatigue performance and indicate that enhanced mechanical properties of ultra-high performance material permits to use a simplified splice details along with short joint width.

Nucleotide Sequence of Mating Locus of Schizophyllum commune Indigenous to North America (북미자생 치마버섯의 Mating Locus의 염기서열)

  • Park, Dong-Cheol;Lee, Sang-Sun;Lee, In-Seon;Kim, Hyeun-Jeong;Lee, Kap-Rang
    • The Korean Journal of Mycology
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    • v.28 no.1
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    • pp.22-25
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    • 2000
  • This study was carried out to compare DNA sequence of mating type locus concerning with direct formation of fruiting body in Schizophyllum commune which is growing in North America with that of same species growing in South America. The nucleotide sequence appeared to have about 96% homology to 1-71 $A{\alpha}3$ allele from South America strain, showing a conservative feature. The polypeptide sequence showed about 82% homology when compared partially with mating activity region of 1-71 $A{\alpha}3$ allele. In addition, this polypeptide sequence indicated 74% and 82% identity in homeodomain and acidic-rich regions known as a transcription factor respectively.

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Fermentation Kinetics for Production of Carotenoids by ${\beta}$-ionone Resistant Mutant of Xanthophyllomyces dendrorhous (Xanthophyllomyces dendrorhous 변이군주에 의한 Carotenoids 생산 발효의 특성 연구)

  • Park, Ki-Moon;Kim, Young-Jun;Song, Min-Woo;Kang, Seog-Jin;Lee, Jae-Heung
    • KSBB Journal
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    • v.21 no.4
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    • pp.286-291
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    • 2006
  • Various ${\beta}$-ionone resistant mutants were isolated from the wild-type red yeast Xanthophyllomyces dendrorhous KCTC 7704. Although the growth of X. dendrorhous KCTC 7704 was strongly inhibited at 0.025 mM ${\beta}$-ionone, one of the ${\beta}$-ionone resistant mutants isolated at 0.1 mM ${\beta}$-ionone by NTG mutagenesis showed rather 70% of relative survival at 0.15 mM ${\beta}$-ionone. Fermentation kinetics study with the mutant was carried out at $20^{\circ}C$ for 4 days in 300-mL baffled flasks. The mutant yielded up to 2.3-fold higher carotenoids content(viz. $1.2{\mu}g$ of total carotenoids per mg of dry cells) compared with the wild-type strain. The production of metabolites such as organic acids could be neglected. Studies on the kinetics with various carbon substrates revealed both an increase in final dry cell mass and a higher total carotenoids content in cell mass with glucose when compared to fructose or sucrose. As a further part of study, the effect of pH on the fermentation kinetics was investigated in glucose-limited chemostat at a dilution rate of $0.04h^{-1}$. When compared to steady-state kinetic parameters obtained at pH 4.0, a significant reduction in cell concentration at pH 3.0 and a lower carotenoids content at pH 5.2 were evident.

Pulsed Field Gel Electrophoresis Profile of Erythromycin-Clindamycin Resistant Streptococcus pyogenes Isolated in Korea (국내분리 Erythromycin-Clindamycin 내성 Streptococcus pyogenes에 대한 Pulsed Field Gel Electrophoresis 양상 분석)

  • Lee, Young-Hee;Hwang, Kyu-Jam;Lee, Kwang-Jun;Park, Kang-Soo;Bae, Song-Mee;Sung, Hwa-Young;Kim, Ki-Sang;Lee, Chong-Sam
    • The Journal of the Korean Society for Microbiology
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    • v.35 no.2
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    • pp.171-180
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    • 2000
  • Ninety two strains of Streptococcus pyogenes were isolated from patients with pharyngitis, scarlet fever, skin infection, and invasive streptococcal infections in Seoul, Korea from January to December, 1998. All isolates were epidemiologically characterized by T protein serotype, and serum opacity factor (OF) detection to phenotypes. To analyze the genetic relationship, fifty two isolates including 32 erythromycin-clindamycin (Em-Cm) resistant strains, 20 antimicrobial susceptible strains were attempted to the pulsed-field gel electrophoresis (PFGE). T protein serotype showed 16 kinds in distribution including T12 and T4. Among the total isolates, 40 strains (43.5%) belonged to the T12 serotype and twenty strains (21.7%) to T4 serotype. On the other hand, when infection aspect of S. pyogenes isolates were analysed by T serotype distribution, T12 type was predominant for pharyngitidis which contributed to 21 strains (53%) and for skin infection isolates which contributed to 11 strains (28%), respectively. In case of T4 type, it was the most predominant pharyngitidis isolates which contributed to 8 strains (40%). In T serotype distribution of Em-Cm resistant strains, 27 strains (84%) of the thirty two showed T12 serotype. In minimum inhibitory concentration (MIC) values of Em-Cm resistance isolates, thirty two isolates showed resistant to erythromycin 27 strains (84%), had high MIC of >$128\;{\mu}g/ml$. And also to clindamycin, twenty two strains (69%) had high MIC of >$128\;{\mu}g/ml$. When OF detection of Em-Cm resistance of S. pyogenes isolates were analyzed by T serotype distribution, T12 serotype isolates revealed that all of the isolates except one strain were OF negative. In PFGE profile analysis to Em-Cm resistance isolates, of the twenty seven, Em-Cm resistance of T12 serotype isolates, 26 strains showed identical PFGE profile and all of these isolates revealed that OF negative. Eighty four percent of Em-Cm resistance S. pyogenes isolates had identical phenotype and PFGE profile. These results strongly suggested that the Em-Cm resistant S. pyogenes isolates from Seoul area showed close genetic correlation and PFGE could be available tool for molecular epidemiology.

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Differentiation of Lentinus edodes Isolates in Korea by Isozyme Polymorphisms and Random Amplified Polymorphic DNA (RAPD) Analysis (Isozyme Polymorphism 및 Random Amplified Polymorphic DNA(RAPD) Pattern에 의한 표고 버섯 품종간 비교)

  • Park, Won-Mok;Ko, Han-Gyu;Park, Ro-Jo;Hong, Ki-Sung;Kim, Gyu-Hyun
    • The Korean Journal of Mycology
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    • v.25 no.3 s.82
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    • pp.176-190
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    • 1997
  • Sixty-three isolates of Lentinus edodes obtained from Korea were used to assess the genetic similarity by isozyme polymorphisms and random amplified polymorphic DNA patterns. The activities of esterase, peroxidase and acid phosphatase displayed 10, 7 and 3 distinct isozyme patterns, respectively. By combining the isozyme patterns obtained with the 3 enzymes, every isolate showed its own distinct electrophoretic phenotypes. A distance matrix calculated between all pairs of 63 electrophoretic phenotypes based on the presence or abscence of isozyme bands were analyzed by the group-average method. Results of the cluster analysis assinged the 63 phenotypes into six major groups. In the analysis of random amplified polymorphic DNA patterns, all isolates of Lentinus edodes were devided into five RAPD groups.

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Preparation and Physical Properties of Poly(ethylene-co-ethyl acrylate)/Carbon Nanotube Nanocomposites (폴리에틸렌에틸아크릴레이트/카본나노튜브 나노복합체의 제조 및 물성)

  • Kook, Jeong Ho;Jeong, Kwang-Un;Yang, Jong Seok;Park, Dae Hee;Go, Jin Hwan;Nah, Changwoon
    • Applied Chemistry for Engineering
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    • v.19 no.2
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    • pp.161-167
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    • 2008
  • Multi-walled carbon nanotubes (MWCNT)-reinforced poly(ethylene-co-ethyl acrylate) (EEA) nanocomposites were prepared by both melt and solution mixing methods. The mechanical, thermal, and electrical properties were investigated as a function of type and loading of CNT. The tensile strength and modulus increased, while elongation at break decreased with increasing MWCNT content. The hollow-type MWCNT showed an improved tensile strength and elongation at break compared with a conventional MWCNT. The thermal degradation temperature was increased by around $40^{\circ}C$ with increasing the amount of MWCNT. The melt-mixed composites showed the highest volume resistivity. In the case of solution-mixed composites, the conventional MWCNT was estimated to show much lower volume resistivity than that of hollow MWCNT. The number and length of extruded CNT onto the fractured surface increased by both increasing the content of CNT and employing the tensile strain to the sample. The melt-mixed specimens showed much smaller number and shorter length of extruded CNT.

Studies on Breeding and Cultivation Characteristics of Lentinula edodes Strains for Sawdust Cultivation (표고 톱밥재배용 균주의 육종과 재배특성에 관한 연구)

  • Ryu, Sung-Ryul;Bak, Won-Chull;Koo, Chang-Duck;Lee, Bong-Hun
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.65-72
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    • 2009
  • For the breeding purpose, in this study we attempted to select a new Lentinula edodes strain by comparing mycelial growth rate, fruit-body quality and mushroom productivity per substrate weight of hybrid strains with parent strains. Nineteen hybrid strains were made by cross of di-karyotic and mono-karyotic to develop strains suitable for sawdust cultivation of L. edodes. Among the nineteen strains, ten were selected by comparing mycelial growth rate on PDA media and wood rot on sawdust-based substrates. The most mushroom harvesting was achieved by 100 days incubation from KFRI 960, KFRI 961, KFRI 962, KFRI 963, KFRI 964, KFRI 971, KFRI 973 and 125 days from KFRI 968, 970. Experiment of incubation periods with the ten strains showed that there were productivity differences between 100 and 150 days incubations. Through comparison of hybrid strains' mushroom productivity it was found that there was no difference in mushroom productivity through crossing high temperature types with high temperature types, crossing low temperature types with high temperature types and crossing unknown temperature types with high temperature types, but there was difference through crossing middle temperature types with high temperature types. Hybrid strains showed better productivity compared with parent strains. Fruiting temperature type analysis of hybrid strains confirmed that strains of high temperature types have a dominant character.

Prevalence of Extended-spectrum β-Lactamase and Quinolone Resistance Genes in Escherichia coli Clinical Isolates and their Antibiotic Resistance (임상검체로부터 분리된 Escherichia coli 의 Extended-spectrum β-lactamase와 퀴놀론 내성 유전자의 출현빈도 및 항생제 내성)

  • Lee, Min Hyeok;Hwang, Yeoung Min;Baik, Keun Sik;Cho, Hyun Wook;Seong, Chi Nam
    • Journal of Life Science
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    • v.23 no.5
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    • pp.703-709
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    • 2013
  • The aim of this study was to investigate the prevalence of Extended-spectrum ${\beta}$-lactamase (ESBL) gene and quinolone resistance determinant (qnr) and the pattern of antibiotic resistance in the ESBL-producing Escherichia coli clinical isolates. The 42 ESBL-producing strains from total 274 isolates were detected using a double disk synergy test. They were isolated from various specimens, such as urine (28 strains), sputum (6 strains), pus (3 strains), wound (2 strains), blood (2 strains), and tissue (1 strain). Using the PCR with the specific primers ESBL, ESBL and qnr gene types were determined. Thirty-five strains possessed one or two ESBL genes. CTX-M-1 type was the most abundant followed by CTX-M-9 type and TEM, but SHV, CTX-M-2, and CTX-M-8 gene types were not detected. qnr gene types were detected from ten isolates in the order of qnrB4, qnrB1, and qnrS. Coexistence of ESBL and qnr genes was found. ESBL-producing isolates showed high resistance against some antibiotics, such as cefotaxmie (80.0%), levofloxacin (82.9%), and ampicillin (100%). Neither a synergy effect from the coexistence of ESBL and qnr genes on antibiotic resistance nor a correlation between the production of qnr gene and quinolone resistance were found.

Fracture Simulation of UHPFRC Girder with the Interface Type Model (경계형 모델을 사용한 초고강도 섬유보강 콘크리트거더의 파괴역학적 해석)

  • Guo, Yi-Hong;Han, Sang-Mook
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.23 no.1
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    • pp.81-94
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    • 2010
  • This paper deals with the fracture simulation of UHPFRC girder with the interface type model. Based on the existing numerical simulation of quasi-brittle fracture in normal strength concrete, constitutive modeling for UHPFRC I-girder has been improved by including a tensile hardening at the failure surface. The finite element formulation is based on a triangular unit, constructed from constant strain triangles, with nodes along its sides and neither at the vertex nor the center of the unit. Fracture is simulated through a hardening/softening fracture constitutive law in tension, a softening fracture constitutive law in shear as well as in compression at the boundary nodes, with the material within the triangular unit remaining linear elastic. LCP is used to formulate the path-dependent hardening-softening behavior in non-holonomic rate form and a mathematical programming algorithm is employed to solve the LCP. The piece-wise linear inelastic yielding-failure/failure surface is modeled with two compressive caps, two Mohr-Coulomb failure surfaces, a tensile yielding surface and a tensile failure surface. The comparison between test results and numerical results indicates this method effectively simulates the deformation and failure of specimen.