• Journal of Aquaculture
• /
• v.17 no.1
• /
• pp.24-29
• /
• 2004

The high mortality of cultured turbot, Scophthalmus maximus was occurred from Gochang in June, 2003. Infect fish with iridovirus showed a lower feed intake and lethargic. These fish exhibited pale body color, extended abdominal and exophthalmus. Histopathological studies showed basophilic enlarged cells from kidney, spleen, gill, heart, muscle, stomach, intestine, liver and pancreas of these fish. In PCR amplification with red sea bream iridovirus (RSIV) specific primer set of a DNA fragment of 286 bp was obtained from infect turbot with the virus. The strain showed the high homologies with RSIV, LBIV (largemouth bass iridovirus), GSIV (giant seaperch iridovirus) and SBIV (sea bass iridovirus).

• Journal of the Korean Society for Precision Engineering
• /
• v.30 no.12
• /
• pp.1281-1286
• /
• 2013

In this paper, a miniaturized stage with two prismatic-prismatic joints (2-PP) type parallel compliant mechanism driven by piezo actuators is proposed. This stage consists of two layers which are a motion guide layer and an actuation layer. The motion guide layer has 2-PP type parallel compliant mechanism to guide two translational motions, whereas the actuation layer has two leverage type amplification mechanisms and two piezo actuators to generate forces. Since the volume of the stage is too small to mount displacement sensors, the piezo actuators embedding strain gauge sensors are chosen. With the strain gauge-embedded piezo actuators, a semi-control is implemented, which results in hysteresis compensation of the stage. As the results, the operating range of $30{\mu}m$, the resolution of 20 nm, and the bandwidth of 400 Hz in each axis were obtained in the experiments.

• Journal of Mushroom
• /
• v.16 no.3
• /
• pp.140-146
• /
• 2018

Pleurotus eryngii is one of the most commercially important mushrooms cultivated in Korea. However, the shelf-life of the fruiting body is short, limiting its export. A new hybrid strain H17 of P. eryngii was developed to extend the shelf-life by mono-mono crossing between monokaryotic strains derived from DanBi and KNR2774. Although the cultivation period of H17 was slightly longer than that of the reference cultivar Kenneutari No.2, the quality did not change and remained normal after a period of 65.0 days at $4^{\circ}C$. This result was significantly different from that of the reference cultivar Kenneutari No.2. Analysis of the genetic characteristics of the new hybrid strain H17 revealed a different profile from that of the parental and reference cultivars when random amplification of polymorphic DNA (RAPD) primers was used. These results demonstrate that H17 is a new cultivar with improved storability after harvesting.

• Journal of Microbiology
• /
• v.41 no.4
• /
• pp.349-352
• /
• 2003

Recent studies have shown that some of the PCB (polychlorinated biphenyl)-degraders are able to effectively degrade PCB in the presence of monoterpenes, which act as inducers for the degradation pathway. Rhodococcus sp. T104, an effective PCB degrader, has been shown to induce the degradation pathway by utilizing limonenes, cymenes, carvones, and pinenes as sole carbon sources which can be found in the natural environment. Moreover, the strain T104 proved to possess three separate oxidation pathways of limonene, biphenyl, and phenol. Of these three, the limonene can also induce the biphenyl degradation pathway. In this work, we report the presence of three distinct genes for aromatic oxygenase, which are putatively involved in the degradation of aromatic substrates including biphenyl, limonene, and phenol, through PCR amplification and denaturing gradient gel electrophoresis (DGGE). The genes were differentially expressed and well induced by limonene, cymene, and plant extract A compared to biphenyl and/or glucose. This indicates that substrate specificity must be taken into account when biodegradation of the target compounds are facilitated by the plant natural substrates.

• International journal of steel structures
• /
• v.18 no.5
• /
• pp.1577-1588
• /
• 2018

As the excellent mechanical performance and easy construction of concrete filled steel tubes (CFST) composite structure, it has the potential to be used to strengthen RC pier columns. Therefore, tests were conducted on 2 reinforcement concrete (RC) stub columns and 9 RC columns strengthened with circular CFST under axial loading. The test results show that the circular CFST strengthening method is effective since the mean bearing capacity of the RC columns is increased at least 3.69 times and the ductility index is significantly improved more than 30%. One of the reasons for enhancement is obvious confinement provided by steel tube besides the additional bearing capacity supplied by the strengthening materials. From the analysis of the enhancement ratio, the strengthening structure has at least an extra 20% amplification except for taking full advantage of the strength of the strengthening material. Through the analysis of confining stress provided by steel tube and the stress-strain relationship of confined concrete, it is found that the strength of the core concrete can be increased by 21-33% and the ultimate strain can be enhanced to beyond $15,000{\mu}{\varepsilon}$.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.12 no.4
• /
• pp.295-306
• /
• 2010

Contrary to an intact rock, the jointed rock mass shows strain-dependent deformation characteristics (elastic modulus and damping ratio). The maximum elastic modulus of a rock mass can be obtained from an elastic wave-based exploration in a small strain level and applied to seismic analyses. However, the assessment and application of the non-linear characteristics of rock masses in a small to medium strain level ($10^{-4}{\sim}0.5%$) have not been carried out yet. A non-linear dynamic analysis module is newly developed for FLAC3D to simulate strain-dependent shear modulus degradation and damping ratio amplification characteristics. The developed module is verified by analyzing the change of the Ricker wave propagation. Strain-dependent non-linear characteristics are obtained from disks of cored samples using a rock mass dynamic testing apparatus which can evaluate wave propagation characteristics in a jointed rock column. Using the experimental results and the developed non-linear dynamic module, seismic analyses are performed for the intersection of a shaft and an inclined tunnel. The numerical results show that vertical and horizontal displacements of non-linear analyses are larger than those of linear analyses. Also, non-linear analyses induce bigger bending compressive stresses acting on the lining. The bending compressive stress concentrates at the intersection part. The fundamental understanding of a strain-dependent jointed rock mass behavior is achieved in this study and the analytical procedure suggested can be effectively applied to field designs and analyses.

• Proceedings of the Computational Structural Engineering Institute Conference
• /
• 1995.04a
• /
• pp.99-106
• /
• 1995

The seismic responses of a base Isolated Pressurized Water Reactor(PWR) are investigated using a mathematical model which expresses the superstructure as a linear lumped mass-spring and the seismic Isolator as an equivalent spring-damper. Time history analyses are performed for the 1940 El Centre earthquake with linear amplification. In the analysis 5% of structural damping is used for the superstructure. The effects of high damping rubber bearing on seismic response of the superstructure in base isolated system are evaluated for four stiffness model types. The acceleration responses in base isolated PWR superstructure with high damping rubber bearings are much smaller than those in fixed base structure. In the higher strain region where stiffness behaves non-linearly, the acceleration responses modelled by one equivalent stiffness are smaller than those in nonlinear spring model, and the higher stiffness spring model of isolator exhibits larger peak acceleration response at superstructure in the frequency range above 2.0 Hz. when subjected to linearly amplified 1940 El Centre earthquake.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.11 no.2
• /
• pp.135-138
• /
• 2005

Two pairs of DNA primers were designed for the detection of the Zhenjiang (China) strain of Bombyx mori densonucleosis virus (BmDNV-Z). These primers were designed from the nucleotide sequence of major structural protein gene (putative VD1-ORF2). PCR amplification was attempted from different issues (including silk gland, blood, skin and midgut) and feces of the silkworm which infected wit BmDNV-Z were amplified by PCR. Both of the primers gave expected size of in the DNA bands from midgut and feces, but not in the DNA of silk gland, blood and skin. The two bands were sequenced, and their sequence were same as the sequence designed for. BmDNV-Z could be successfully detected in single silkworm after it was infected for 12 hrs, and could not be detected before 9 hrs after infected.

• Korean Journal of Soil Science and Fertilizer
• /
• v.48 no.2
• /
• pp.119-124
• /
• 2015

Pyoverdines (PVDs) are organic compounds produced by the fluorescent Pseudomonads under iron starvation conditions. Among the isolated rhizosphere pseudomonads strains, P. putida KNUK9 showed the highest production of PVDs and its production reached to 62.81% siderophores units. DNA isolation, ligation, PCR amplification, and transformation using E. coli $DH5{\alpha}$ cells were carried out for preparing the strong pyoverdine producer strains. We detected seven genes playing the fundamental roles in the pyoverdine metabolism in Pseudomonads. According to data and analysis obtained from the study, we deduced that the strain P. putida KNUK9 contains the essential genes required for pyoverdine biosynthesis.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.3
• /
• pp.558-562
• /
• 2010

SDS-PAGE of extracted surface-associated proteins of Lactobacillus rhamnosus strains E/N, Oxy, and Pen, was performed. The obtained protein patterns allowed differentiation of the examined strains, which was not accomplished by the commonly used RAPD genotypic method. The differentiation by the SDS-PAGE method proved to be a useful tool for strain-specific identification, which was further confirmed by 2DE analysis. Therefore, it can be used as an alternative or complementary method for both conventional and genotypic identification procedures, especially when closely related lactobacilli isolates are identified.