Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Isolation and Detection of Genes Responsible for Pyoverdines Biosynthesis in Pseudomonas putida KNUK9

  • Hussein, Khalid A. (Department of Biological Environment, Kangwon National University) ;
  • Joo, Jin Ho (Department of Biological Environment, Kangwon National University)
  • Received : 2015.03.19
  • Accepted : 2015.04.10
  • Published : 2015.04.30
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Abstract

Pyoverdines (PVDs) are organic compounds produced by the fluorescent Pseudomonads under iron starvation conditions. Among the isolated rhizosphere pseudomonads strains, P. putida KNUK9 showed the highest production of PVDs and its production reached to 62.81% siderophores units. DNA isolation, ligation, PCR amplification, and transformation using E. coli $DH5{\alpha}$ cells were carried out for preparing the strong pyoverdine producer strains. We detected seven genes playing the fundamental roles in the pyoverdine metabolism in Pseudomonads. According to data and analysis obtained from the study, we deduced that the strain P. putida KNUK9 contains the essential genes required for pyoverdine biosynthesis.

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References

  1. Ackerley D.F., Caradoc-Davies T.T., Lamont I.L. (2003). Substrate specificity of the nonribosomal peptide synthetase PvdD from Pseudomonas aeruginosa. J Bacteriol 185:2848-2855. https://doi.org/10.1128/JB.185.9.2848-2855.2003
  2. Alexander, D.B., Zuberer, D.A. (1991). Use of chrome azurol s regents to evaluate siderophore production by rhizosphere bacteria. Biol. Fertils. Soils., 12:39-45. https://doi.org/10.1007/BF00369386
  3. Beare, P.A., For, R.J., Martin, L.W., Lamont, I.L. (2003). Siderophore-mediated cell signalling in Pseudomonas aeruginosa: divergent pathways regulate virulence factor production and siderophore receptor synthesis. Mol. Microbiol. 47:195-207.
  4. Brosies, J.M., Palmer, L., Kennedy, P., Noller, H.F., (1978). Complete nucleotide sequence of the 16S ribosomal DNA gene from Escherichia coli. Proc. Natl. Acad. Sci. USA 75: 4801-4805. https://doi.org/10.1073/pnas.75.10.4801
  5. Espinosa-Urgel, M., Kolter, R., and Ramos, J.L. (2002). Root colonization by Pseudomonas putida: love at first sight. Microbiology 148:341-343. https://doi.org/10.1099/00221287-148-2-341
  6. Hussein, K.A. and Joo, J.H. (2014). Potential of Siderophore Production by Bacteria Isolated from Heavy Metal: Polluted and Rhizosphere Soils. Current Microbiology, 68(6):717-723. https://doi.org/10.1007/s00284-014-0530-y
  7. King, E.O., WARD, M.K., RANEY, D.E. (1954). PMIDMedia for the demonstration of pyocyanin and fluorescein, J. Lab. Clin. Med. 44:301.
  8. Kovacevic, S., Tobin, M.B., Miller, J.R. (1990). The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus. J Bacteriol 172:3952-3958. https://doi.org/10.1128/jb.172.7.3952-3958.1990
  9. Lamont, I.L., Beare, P.A., Ochsner, U., Vasil, A.I., Vasil, M.L. (2002). Siderophore-mediated signaling regulates virulence factor production in Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. U.S.A. 99:7072-7077. https://doi.org/10.1073/pnas.092016999
  10. Machuca, A., Milagres, A.M.F. (2003). Use of CAS-agar plate modified to study the effect of different variables on siderophore production by Aspergillus. Lett. Appl. Microbiol., 36:177-181. https://doi.org/10.1046/j.1472-765X.2003.01290.x
  11. Merriman, T.R., Merriman, M.E., Lamont, I.L. (1995). Nucleotide sequence of pvdD, a pyoverdine biosynthetic gene from Pseudomonas aeruginosa: PvdD has similarity to peptide synthetases. J Bacteriol 177:252-258. https://doi.org/10.1128/jb.177.1.252-258.1995
  12. Meyer, J.M., Abdallah, M.A. (1978). The fluorescent pigment of Pseudomonas fluorescens: biosynthesis, purification and physicochemical properties. J Gen Microbiol 107:319-328. https://doi.org/10.1099/00221287-107-2-319
  13. Mossialos, D., Ochsner, U., Baysse, C., Chablain, P., Pirnay, J.P., Koedam, N., Budzikiewicz, H., Fernández, D.U., Schäfer, M., Ravel, J., Cornelis, P. (2002). Identification of new, conserved, nonribosomal peptide synthetases from fluorescent pseudomonads involved in the biosynthesis of the siderophore pyoverdine. Mol. Microbiol. 45:1673-1685. https://doi.org/10.1046/j.1365-2958.2002.03120.x
  14. Mossialos, D., Baysse, C., Ochsner, U., Chablain, P., Pirnay, J.P., Koedam, N., Budzikiewicz, H., Fernandez, D.U., Schafer, M., Ravel, J., Cornelis, P. (2002). Identification of new, conserved, non-ribosomal peptide synthetases from fluorescent pseudomonads involved in the biosynthesis of the siderophore pyoverdine. Mol Microbiol 45:1673-1685. https://doi.org/10.1046/j.1365-2958.2002.03120.x
  15. Nelson, K.E., Nelson, K.E., Weinel, C., Paulsen, I.T., Dodson, R.J., Hilbert, H., Martins dos Santos, V.A., Fouts, D.E., Gill, S.R., Pop, M., Holmes, M., Brinkac, L., Beanan, M., DeBoy, R.T., Daugherty, S., Kolonay, J., Madupu, R., Nelson, W., White, O., Peterson, J., Khouri, H., Hance, I., Chris Lee, P., Holtzapple, E., Scanlan, D., Tran, K., Moazzez, A., Utterback, T., Rizzo, M., Lee, K., Kosack, D., Moestl, D., Wedler, H., Lauber, J., Stjepandic, D., Hoheisel, J., Straetz, M., Heim, S., Kiewitz, C., Eisen, J.A., Timmis, K.N., Düsterhöft, A., Tummler, B., Fraser, C.M. (2002). Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environ. Microbiol. 4:799-808. https://doi.org/10.1046/j.1462-2920.2002.00366.x
  16. Ochsner, U., Wilderman, P.J., Vasil, A.I., Vasil, M.L. (2002). Gene Chipw expression analysis of the iron starvation response in Pseudomonas aeruginosa: identification of novel pyoverdine biosynthesis genes. Mol. Microbiol. 45:1277-1287. https://doi.org/10.1046/j.1365-2958.2002.03084.x
  17. Ravel, J., Cornelis, P. (2003). Genomics of pyoverdine-mediated iron uptake in pseudomonads. Trends Microbiol 11:195-200. https://doi.org/10.1016/S0966-842X(03)00076-3
  18. Redly, G.A. and Poole, K. (2003). Pyoverdine-mediated regulation of FpvA synthesis in Pseudomonas aeruginosa: Involvement of a probable extracytoplasmic-function sigma factor, FpvI. J. Bacteriol. 185:1261-1265. https://doi.org/10.1128/JB.185.4.1261-1265.2003
  19. Regenhardt, D., Heuer, H., Heim, S., Fernandez, D.U., Strömpl, C., Moore, E.R.B., and Timmis, K.N. (2002). Pedigree and taxonomic credentials of Pseudomonas putida strain KT2440. Environ Microbiol 4:912-915. https://doi.org/10.1046/j.1462-2920.2002.00368.x
  20. Schwyn, B., Neilands, J.B. (1987). Universal chemical assay for the detection and determination of siderophores. Anal. Biochem., 160:47-56. https://doi.org/10.1016/0003-2697(87)90612-9
  21. Spencer, H. Spencer David, H., Kas Arnold, Smith Eric E, Raymond Christopher K, Sims Elizabeth H, Hastings Michele, Burns Jane L, Kaul Rajinder, Olson Maynard V. (2003). Whole-genome sequence variation among multiple isolates of Pseudomonas aeruginosa. J. Bacteriol. 185:1316-1325.
  22. Zhang, J.H., Quigley, N.B., Gross, D.C. (1997). Analysis of the syrP gene, which regulates syringomycin synthesis by Pseudomonas syringae pv. syringae. Appl. Environ. Microbiol. 63:2771-2778.