• Title/Summary/Keyword: Strain Amplification

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Loop-Mediated Isothermal Amplification for the Detection of Xanthomonas arboricola pv. pruni in Peaches

  • Li, Weilan;Lee, Seung-Yeol;Back, Chang-Gi;Ten, Leonid N.;Jung, Hee-Young
    • The Plant Pathology Journal
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    • v.35 no.6
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    • pp.635-643
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    • 2019
  • To detect Xanthomonas arboricola pv. pruni, a loopmediated isothermal amplification (LAMP) detection method were developed. The LAMP assay was designed to test crude plant tissue without pre-extraction, or heating incubation, and without advanced analysis equipment. The LAMP primers were designed by targeting an ABC transporter ATP-binding protein, this primer set was tested using the genomic DNA of Xanthomonas and non-Xanthomonas strains, and a ladder product was generated from the genomic DNA of X. arboricola pv. pruni strain but not from 12 other Xanthomonas species strains and 6 strains of other genera. The LAMP conditions were checked with the healthy leaves of 31 peach varieties, and no reaction was detected using either the peach leaves or the peach DNA as a template. Furthermore, the high diagnostic accuracy of the LAMP method was confirmed with 13 X. arboricola pv. pruni strains isolated from various regions in Korea, with all samples exhibiting a positive reaction in LAMP assays. In particular, the LAMP method successfully detected the pathogen in diseased peach leaves and fruit in the field, and the LAMP conditions were proven to be a reliable diagnostic method for the specific detection and identification of X. arboricola pv. pruni in peach orchards.

Preliminary data analysis of surrogate fuel-loaded road transportation tests under normal conditions of transport

  • JaeHoon Lim;Woo-seok Choi
    • Nuclear Engineering and Technology
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    • v.54 no.11
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    • pp.4030-4048
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    • 2022
  • In this study, road transportation tests were conducted with surrogate fuel assemblies under normal conditions of transport to evaluate the vibration and shock load characteristics of spent nuclear fuel (SNF). The overall test data analysis was conducted based on the measured acceleration and strain data obtained from the speed bump, lane-change, deceleration, obstacle avoidance, and circular tests. Furthermore, representative shock response spectrums and power spectral densities of each test mode were acquired. Amplification or attenuation characteristics were investigated according to the load transfer path. The load attenuated significantly as it transferred from the trailer to the cask. By contrast, the load amplified as it transferred from the cask to the surrogate SNF assembly. The fuel loading location on the cask disk assembly did not exhibit a significant influence on the strain measured from the fuel rods. The principal strain was in the vertical direction, and relatively large strain values were obtained in spans with large spacing between spacer grids. The influence of the lateral location of fuel rods was also investigated. The fuel rods located at the side exhibited relatively large strain values than those located at the center. Based on the strain data obtained from the test results, a hypothetical road transportation scenario was established. A fatigue evaluation of the SNF rod was performed based on this scenario. The evaluation results indicate that no fatigue damage occurred on the fuel rods.

G protein of infectious hematopietic necrosis virus (IHNV) isolated from adult rainbow trout, Salmo gairdneri in Korea (국내의 양식산 무지개송어 성어로부터 분리된 IHNV의 G protein gene에 대한 연구)

  • Kim, Gi-Hong;Kim, Wi-Sik;Kim, Chun-Seop;Kim, Yeong-Jin;Jeong, Tae-Seong;;Jeong, Seong-Ju;O, Myeong-Ju
    • Journal of fish pathology
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    • v.16 no.3
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    • pp.161-164
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    • 2003
  • An Infectious hematopoietic necrosis virus strain (IHNV-RTK) was isolated from cultured rainbow trout at Kumi and Jechun area in Korea during 2000 and 2001. In the RT-PCR amplification with the specific primer set designed from IHNV G protein region, a 540 bp PCR product was amplified from the RTK strain. The RTK strain showed higher sequence homology with the published IHNV G protein genes (RB-76, LR-73, Col-85, and Carson-89)

Young's Modulus Measurement of FeCoGe/phenol Composites (FeCoGe/페놀 복합체의 영률 측정)

  • Park, K.I.;Kim, H.G.;Shin, K.H.;SaGong, G.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.08a
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    • pp.107-109
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    • 2003
  • The magnetostriction of FeCoGe/phenol composites is measured under the external magnetic field. A few Measurement are carried out by using the electrical-resistance strain gage, the Wheaton Bridge for eliminating the unnecessary voltage, the lock-in-amplifier for the signal amplification and noise filtering. When the external magnetic field is applied to the longitudinal direction against those samples which is the 10wt.% phenol in composites, the theoretical maximum strain of 120ppm is obtained. According to the larger strain than that of others solid state actuators and piezoelectric actuators. FeCoGe/phenol composites could be useful as an actuator.

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Magnetostriction and Young's Modulus Measurement of FeCoGeW/phenol Composites (FeCoGeW/페놀 복합체의 자왜 및 영률 측정)

  • Park, K.I.;Kim, H.G.;Shin, K.H.;SaGong, G.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.11a
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    • pp.325-328
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    • 2003
  • The magnetostriction of FeCoGeW/phenol composites is measured under the external magnetic field. A few Measurement are carried out by using the electrical-resistance strain gage, the Wheaton Bridge for eliminating the unnecessary voltage, the lock-in-amplifier for the signal amplification and noise filtering. When the external magnetic field is applied to the longitudinal direction against those samples which is the 10wt.% phenol in composites, the theoretical maximum strain of 120ppm is obtained. According to the larger strain than that of others solid state actuators and piezoelectric actuators. FeCoGeW/phenol composites could be useful as an actuator.

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A Plan to Develop Seismic Capacity Verification Procedures Based on the Elastic-Plastic Strain Features (탄소성 변형률 기반 내진성능 평가 절차서 개발 방안)

  • Hwang, Jong Keun;Jeong, Ill Seok;Kim, Beom Shig;Ahn, Sang Won;Bang, Hye Jin;Lee, Min Hee;Jeong, Hyeon Seob
    • Transactions of the Korean Society of Pressure Vessels and Piping
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    • v.14 no.2
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    • pp.11-15
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    • 2018
  • A development plan for seismic capacity verification procedures of nuclear components based on the elastic-plastic strain (EPS) features is explained in this paper. The EPS methodology is more realistic to assess seismic responses of components to extreme seismic events beyond the safe shutdown earthquake (SSE) than current practices with the criteria of stress limits. The EPS based approach to analyze the seismic capacity of components can reduce over-conservatism in the current stress-based criteria and can incorporate the seismic responses of components deformed in plastic behavior by the motion of extreme earthquake.

Data analysis of simulated fuel-loaded sea transportation tests under normal conditions of transport

  • JaeHoon Lim;Woo-seok Choi
    • Nuclear Engineering and Technology
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    • v.56 no.2
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    • pp.375-388
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    • 2024
  • In this study, to evaluate the shock and vibration load characteristics of used fuel, a sea transportation test was conducted using simulated fuel assemblies under normal transport conditions. An overall test data analysis was performed based on the measured strain and acceleration data obtained from cruise, rotation, acceleration, braking, depth of water, and rolling tests. In addition, shock response spectrum and power spectral densities were obtained for each test case. Amplification and attenuation characteristics were investigated based on the load path. The load was amplified as it passed from the overpack to the simulated used fuel-assembly. As a result of the RMS trend analysis, the fuel-loading position of the transportation package affected the measured strain in the fuel rod, and the maximum strains were obtained at the spans with large spacing. However, even these maximum strains were very small compared to the fatigue strength and the cladding yield strength. Moreover, the fuel rods located on the side exhibited a larger strain value than those at the center.

Effect of Pressure on Acoustic Pressure Response and NO Formation in Diluted Hydrogen-Air Diffusion Flames (희석된 수소-공기 확산 화염에서 음향파 응답과 NO 생성에 미치는 압력의 영향)

  • Sohn, Chae-Hoon;Chung, Suk-Ho
    • 한국연소학회:학술대회논문집
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    • 1999.10a
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    • pp.11-20
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    • 1999
  • Acoustic pressure response and NO formation of hydrogen-air diffusion flames at various pressures are numerically studied by employing counterflow diffusion flame as a model flame let in turbulent flames in combustion chambers. The numerical results show that extinction strain rate increases linearly with pressure and then decreases, and increases again at high pressures. Thus, flames are classified into three pressure regimes. Such non-monotonic behavior is caused by the change in chemical kinetic behavior as pressure rises. Acoustic pressure response in each regime is investigated based on the Rayleigh criterion. At low pressures, pressure-rise causes the increase in flame temperature and chain branching/recombination reaction rates, resulting in increased heat release. Therefore, amplification in pressure oscillation is predicted. Similar phenomena are predicted at high pressures. At moderate pressures, weak amplification is predicted. Emission index of NO shows similar behaviors as to the peak-temperature variation with pressure.

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Dynamic simulation models for seismic behavior of soil systems - Part I: Block diagrams

  • Sahin, Abdurrahman
    • Geomechanics and Engineering
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    • v.9 no.2
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    • pp.145-167
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    • 2015
  • Digital simulation has recently become the preferred method for designing complex and dynamic systems. Simulation packages provide interactive, block-diagram environment for modeling and simulating dynamic models. The block diagrams in simulation models are flowcharts which describe the components of dynamic systems and their interaction. This paper is the first part of the study for determining the seismic behavior of soil systems. The aim of this part is to present the constructed block diagrams for discrete-time analysis of seismic site amplification in layered media for vertically propagating shear waves. Detailed block diagrams are constructed for single and multiple soil layers by considering wave propagation with and without damping, respectively. The block diagrams for recursive filter to model attenuation in discrete-time form are also constructed. Finite difference method is used for strain calculation. The block diagrams are developed by utilizing Simulink which is a software add-on to Matlab.

Design, Fabrication and Characterization of Lateral PZT actuator using Stiffness Control (강성제어 구조물을 이용한 수평구동형 박막 PZT 엑츄에이터의 설계, 제작 및 특성평가)

  • 서영호;최두선;이준형;이택민;제태진;황경현
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2004.10a
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    • pp.756-759
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    • 2004
  • We present a piezoelectric actuator using stiffness control and stroke amplification mechanism in order to make large lateral displacement. In this work, we suggest stiffness control approach that generates lateral displacement by increasing the vertical stiffness and reducing the lateral stiffness using additional structure. In addition, an additional structure of a serpentine spring amplifies the lateral displacement like leverage structure. The suggested lateral PZT actuator (bellows actuator) consists of serpentine spring and PZT/electrode layer which is located at the edge of the serpentine spring. The edge of the serpentine spring prevents the vertical motion of PZT layer, while the other edge of the serpentine spring makes stroke amplification like leverage structure. We have determined dimensions of the bellows actuator using ANSYS simulation. Length, width and thickness of PZT layer are 135$\mu$m, 20$\mu$m and 0.4$\mu$m, respectively. Dimensions of the silicon serpentine spring are thickness of 25$\mu$m, length of 300$\mu$m, and width of 5$\mu$m. The bellows actuator has been fabricated by SOI wafer with 25$\mu$m-top silicon and 1$\mu$m-buried oxide layer. The bellows actuator shows the maximum 3.93$\pm$0.2$\mu$m lateral displacement at 16V with 1Hz sinusoidal voltage input. In the frequency response test, the fabricated bellows actuator showed consistent displacement from 1Hz to 1kHz at 10V. From experimental study, we found the bellows actuator using thin film PZT and silicon serpentine spring generated mainly laterally displacement not vertical displacement at 16V, and serpentine spring played role of stroke amplification.

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