• Molecules and Cells
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• v.44 no.10
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• pp.770-779
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• 2021

Transgenic Arabidopsis thaliana expressing an anti-rabies monoclonal antibody (mAb), SO57, was obtained using Agrobacterium-mediated floral dip transformation. The endoplasmic reticulum (ER) retention signal Lys-Asp-Glu-Leu (KDEL) was tagged to the C-terminus of the anti-rabies mAb heavy chain to localize the mAb to the ER and enhance its accumulation. When the inaccurately folded proteins accumulated in the ER exceed its storage capacity, it results in stress that can affect plant development and growth. We generated T₁ transformants and obtained homozygous T₃ seeds from transgenic Arabidopsis to investigate the effect of KDEL on plant growth. The germination rate did not significantly differ between plants expressing mAb SO57 without KDEL (SO plant) and mAb SO57 with KDEL (SOK plant). The primary roots of SOK agar media grown plants were slightly shorter than those of SO plants. Transcriptomic analysis showed that expression of all 11 ER stress-related genes were not significantly changed in SOK plants relative to SO plants. SOK plants showed approximately three-fold higher mAb expression levels than those of SO plants. Consequently, the purified mAb amount per unit of SOK plant biomass was approximately three times higher than that of SO plants. A neutralization assay revealed that both plants exhibited efficient rapid fluorescent focus inhibition test values against the rabies virus relative to commercially available human rabies immunoglobulins. KDEL did not upregulate ER stress-related genes; therefore, the enhanced production of the mAb did not affect plant growth. Thus, KDEL fusion is recommended for enhancing mAb production in plant systems.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.4
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• pp.587-593
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• 2021

Since 2017, a total of 29 fire accidents have occurred in energy storage systems (ESSs) as of June 2020. The common mode voltage (CMV) is one of the electrical hazards that is assumed to be a cause of those fire accidents. Several cases of CMV that violate the allowable insulation level of a battery section are being reported in actual ESS operation sites with △-Y winding connections. Thus, this paper evaluates the characteristics of CMV. An ESS site was modeled with an AC grid, PCS, and battery sections using PSCAD/EMTDC software. As a result of a simulation based on the proposed model, it was confirmed that characteristics of CMV vary significantly and are similar to actual measurements, depending on the grounding method of the internal transformer for PCS. The insulation level of the battery section may be severely degraded as the value of CMV exceeds the rated voltage in case of a grounding connection. It was found that the value of CMV dramatically declines when the internal transformer for PCS is operated as non-grounding connection, so it meets the standard insulation level.

• Journal of the Korean Applied Science and Technology
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• v.35 no.4
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• pp.1260-1268
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• 2018

To improve fuel performance and specific characteristics of long storage and moving through fuel systems additives should be added in kerosene type aviation turbine fuel (AVTUR) such as antioxidant, fuel system icing inhibitor (FSII), electric conductivity improvers and so on. The dosage of additives has to be analyzed qualitatively and quantitatively due to inspect the quality of abnormal fuel and distinguish other petroleum products. Multi-dimensional GC-MS (MDGC-MS) with Deans switching technique are applied the determination of antioxidant and FSII, which are added with AVTUR containing complex mixture of hydrocarbons. Antioxidant and FSII in the range of 2.5-20 mg/L was quantitatively and qualitatively analyzed using MDGC-MS and the detection limit was about twice as low as that of the 1-dimensional GC-MS results. The method in this study has been higher peak resolution compared with GC-MS and could be simultaneously analyzed different two additives without sample pre-treatment.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.2
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• pp.36-42
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• 2019

Europe and North America are paying attention to residential ESS(Energy Storage System) that can manage energy efficiently. The ESS is a system that stores and manages the electric power by charging and discharging the battery. The ESS is generally used in conjunction with photovoltaic systems. The ESS supplies the load of the power generation time and stores the remaining PV power to supply the load at the non-power generation time. However, due to the high price of residential ESS, low electric rates and increasing block rates, there is no market of residential ESS in Korea. This paper reviews the price condition and the capacity for applying PV and residential ESS to household of apartments using HOMER in Korea.

• The Korean Journal of Archival Studies
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• no.56
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• pp.81-112
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• 2018

Government publications at public institutions are data that are intended to convey the purpose of an institution or the performance of its business to the public or inside the institution. The management of government publications has become increasingly important under the framework of laws and systems, but matters concerning the nature and value of government publications have not been carried out at records management sites. The Act focused on the archives of the six metropolitan government, and presented issues and improvement measures for efficient management of government publications. To that end, the government should maintain a consistent payment system for publications. As payment method and payment book data in government publications are presented differently by law, a system for integrated management should be established to provide brief information and original information. Second, it is necessary to establish an administrative system that meets the storage environment. The publication registration and payment book system presented by the government's publication guidelines is the central system of the National Archives and Records Service, so it is not an efficient system in the agency archives. Third, support should be provided to recognize the value of government publications and to preserve and manage them in the long term. To dispose of the government publications, a realistic classification method, integrated and controlled program should be presented, and criteria for users using the service should be provided. Fourth, it is the establishment of an integrated management system for government publications. The National Archives Service, the National Central Library, and the National Assembly Library should simultaneously build an abstract of information and original information so that they can be managed systematically and efficiently.

• Journal of Wetlands Research
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• v.21 no.2
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• pp.181-190
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• 2019

Recently, Low impact development techniques, a form of nature-based solutions (NBS), were seen cost-efficient alternatives that can be utilized as alternatives for conventional stormwater management practices. This study evaluated the effectiveness of an infiltration trench (IT) and a small constructed wetland (SCW) in treating urban stormwater runoff. Long-term monitoring data were observed to assess the seasonal performance and cite the advantages and disadvantages of utilizing the facilities. Analyses revealed that the IT has reduced performance during the summer season due to higher runoff volumes that exceeded the facility's storage volume capacity and caused the facility to overflow. On the other hand, the pollutant removal efficiency of the SCW was impacted by the winter season as a result of dormant biological activities. Sediment data also indicated that fine and medium sand particles mostly constituted the trapped sediments in the pretreatment and media zones. Sediments in SCW exhibited a lower COD and TN load due to the phytoremediation and microbiological degradation capabilities of the system. This study presented brief comparison LID facilities equipped with pre-treatment zones. The identified factors that can potentially affect the performance of the systems were also beneficial in establishing metrics on the utilization of similar types of nature-based stormwater management practices.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.15 no.7
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• pp.2377-2398
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• 2021

In the recent modernized world, utilization of natural resources (renewable & non-renewable) is increasing drastically due to the sophisticated life style of the people. The over-consumption of non-renewable resources causes pollution which leads to global warming. Consequently, government agencies have been taking several initiatives to control the over-consumption of non-renewable natural resources and encourage the production of renewable energy resources. In this regard, we introduce an IoT powered integrated framework called as green home architecture (GHA) for green score calculation based on the usage of natural resources for household purpose. Green score is a credit point (i.e.,10 pts) of a family which can be calculated once in a month based on the utilization of energy, production of renewable energy and pollution caused. The green score can be improved by reducing the consumption of energy, generation of renewable energy and preventing the pollution. The main objective of GHA is to monitor the day-to-day usage of resources and calculate the green score using the proposed green score algorithm. This algorithm gives positive credits for economic consumption of resources and production of renewable energy and also it gives negative credits for pollution caused. Here, we recommend a green score based tax calculation system which gives tax exemption based on the green score value. This direct beneficiary model will appreciate and encourage the citizens to consume fewer natural resources and prevent pollution. Rather than simply giving subsidy, this proposed system allows monitoring the subsidy scheme periodically and encourages the proper working system with tax exemption rewards. Also, our GHA will be used to monitor all the household appliances, vehicles, wind mills, electricity meter, water re-treatment plant, pollution level to read the consumption/production in appropriate units by using the suitable sensors. These values will be stored in mass storage platform like cloud for the calculation of green score and also employed for billing purpose by the government agencies. This integrated platform can replace the manual billing and directly benefits the government.

• Journal of Korean Society of Forest Science
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• v.100 no.2
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• pp.232-239
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• 2011

This study was performed to find out the persimmon vinegar as a functional drink on reducing blood lipids and enhancing exercise performance. For these, thirty two Sprague-Dawley male rats were divided into 4 groups; control (CONT), water placebo with high fat diet control (ACON), high fat diet with 2.5 times diluted persimmon vinegar ingestion (PV2.5), and high fat diet with 5.0 times diluted persimmon vinegar ingestion (PV5.0). Body weight was not different. Abdominal fat pads were statistically reduced in PV2.5 and PV5.0 compared to CONT and ACON. Blood glucose was not significant but TC, LDL-C and TG were lower in PV2.5 and PV5.0 than other groups, and HDL-C in PV2.5 was the highest among groups. Glycogen contents in the muscle and liver were higher in PV2.5 and PV5.0 compared to CONT and ACON. These results suggested that persimmon vinegar ingestion may inhibit the blood lipids increase and increase glycogen storage which possibly enhancing exercise performance. Therefore, persimmon vinegar has the possibility as the functional drink.

• (The)Study of the Eastern Classic
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• no.73
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• pp.281-302
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• 2018

The role of mind is very important in Xunzi's thought. The way is permanent rule of politics that is under the control of mind which is recognizing organ for both thinking and acknowledging things in his thought. Nevertheless the mind in Xunzi's thought has desire as an organ of body to be controlled. On the other hand, the mind has conflicting two fold aspects in the course of its recognition of things like storage and vacancy, movement and calmness, and two kinds of recognizing opinion against one object. And there are various evils in thinking system of mind. In a sense, the mind of human is to be cleaned and cultivated. Xunzi proposed managing energy and cultivating mind as a thinking system. Mengzi proposed to enliven vast-flowing energy and to cultivate mind through shortening desire, while Xunzi did managing energy and caring about mind for controlling other body systems and publicly recognizing things. In Xunzi's idea the mind in the recognition of things is similar to the water in bowl as a mirror for looking at any things, and thus it is imperative for us to sink grounds in mind for becoming good water mirror. In this need the grounds of human mind would be to be sunk and also the cultivation of it would be to be demanded. Ultimately there should need the recognition of mind that is cleaned and cultivated for brightly discovering the great principle and recovering the unchangeable way with a view to preparing the condition for national peace. This thesis, in this context, to approach the meaning of mind, its two fold aspects, and the necessity of it.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.12
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• pp.339-345
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• 2020

In the field of defense, one-shot devices such as missiles are stored for a long period of time after they are manufactured, so it is essential to predict their storage life. A study was conducted to find the shelf life of a Li-ion battery used in one-shot devices. To do this, a Li-ion battery that has been used in weapon systems for more than 5 years was secured. A non-functional test was performed on the battery to check for external changes or failures. After the non-functional test, a discharge test was performed to measure the performance after storing it. Through the test, the performance was checked, including the initial charging voltage, discharge time, and battery temperature, and the trend of the change was identified. An F-test, One-way ANOVA, and regression analysis were performed to verify the aging, and the shelf life of the battery was estimated by an approximation formula that was derived through a regression analysis. As a result of the ANOVA, the p-value was less than the reference value of 0.05, and the performance of the battery decreased by more than 15% after a certain period of time. This change is assumed to result from the change in physical properties of the lithium polymer cell.