• Title/Summary/Keyword: Storage Buffer

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Characterization of cytosine deaminase with substrate specificity to 5-fluorocytosine (5-fluorocytosine에 기질특이성을 가지는 cytosine deaminase의 특성)

  • Yeeh, Yeehn;Park, Chan-Young
    • Korean Journal of Microbiology
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    • v.26 no.3
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    • pp.207-214
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    • 1988
  • A cytosine deaminase from the cell-free extract of an isolate was examined after ethyl alcohol reactionation. The enzyme catalyzed the conversion of 5-fluorocytosine to 5-fluorouracil by the possession of specificity to the substrate. The optimum temperature and storage time on the stability of the enzyme were at below $50^{\circ}C$ and near 2 days in tris-HCl buffer. The maximum activity was also presented ar 9.0 in pH and $45^{\circ}C$ in temperature. The pHs and temperatures for the enzyme activity ranged from 8.5-9.5 and from 40-$50^{\circ}C$, respectively. the presence of $Ag^{+}, Hg^{2+}, Zn^{2+}$ in the reaction mixture resulted in the marked inhibition in the activity, but 1mM of $Fe^{3+}, K^{+}$, or $Na^{+}$ increased the enzyme activity. The enzyme preparation was vot affected by inhibitors used except N-ethylmaleimide of 1 and 10mM, and considerably activated by 1mM of pyrophosphate and 10mM of phosphate.

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An Ultra-Lightweight RFID Authentication Protocol Using Index (인덱스를 사용한 초경량 RFID 인증 프로토콜)

  • Lee, Jae-Kang;Oh, Se-Jin;Yun, Tae-Jin;Chung, Kyung-Ho;Ahn, Kwang-Seon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.37 no.1C
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    • pp.24-33
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    • 2012
  • Recently, the ultra-lightweight authentication RFID protocol that can actually implement on the RFID Tag is one among authentication protocols getting a concern, but recently many problems were clarified of the feature becase of the protocol which doesn't use the security algorithm. In this paper, we analyzed the problem of the ultra-lightweight authentication protocols and propose the design of ultra-lightweight RFID authentic ation protocols improving the index processing techniques. Because of improving the index processing technique in the method sending the Server authentication message to the authenticated tag, the proposed protocol is strong against the active attack which Li presents. Besides, the proposed protocol has the buffer storage of the keys and index and is strong against the asynchronous attack.

A Study on the Social Implication and Spatial Composition Factor of Rural Apartment Housing (농촌형 공동주택의 사회적 함의와 공간구성 요소에 관한 연구)

  • Kim, Kang-Sub;Lee, Sang-Jung
    • Journal of the Korean Institute of Rural Architecture
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    • v.10 no.1
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    • pp.51-58
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    • 2008
  • This study examined the present condition and problems of rural apartment house through documentary survey and considered the social necessity and concept of rural apartment housing. The aim of this study is to suggest the social necessity and spatial composition factor of rural apartment housing. The results of this study are as follows. First, rural apartment houses are generally adjacent to production and natural environments, and tend to be constructed in small complex of 50 households or less with the lack of convenient facilities. Second, the ultimate goal of rural apartment housing plans is to improve the livability, community, sustainability and rurality on the basis of the basic direction of rural areas. Third, it is necessary to install storages within the apt. complex and secure a buffer zone where people can wash their hands before entering the houses. Fourth, rural apartment housing should adopt the space components of auxiliary facilities including storages for agricultural products and farming appliances, common workshops, field attached to home, and washrooms in consideration of the conditions of rural areas different from urban apartment housing.

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An analysis on the network performance for VoD services using multicast (멀티캐스트를 이용한 VoD 서비스망의 성능 분석)

  • 백남균;정수환;민병준
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.6A
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    • pp.918-926
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    • 2000
  • A huge storage and broad bandwidth are required to provide subscribers with VoD services. Hence, there is a huge amount of cost in establishing networks for VoD services. To determine how to reduce the cost, a number of studies involving such as distributed server systems, multicasting, program caching, stream sharing, are currently in progress. In this paper, a request criterion for multicast service and the ratio of required bandwidth for unicast to multicast are generated. The effect of multicast service on network bandwidth, server bandwidth, and buffer size was analyzed and validated through computer simulation. The results of this study could be applied to efficient designing networks for VoD services.

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Effect of stigmasterol in liposome bilayer on the stabilization of encapsulated ascorbic acid (리포솜 이중층의 stigmasterol이 포집된 ascorbic acid의 안정성에 미치는 영향)

  • Lee, Dong-Uk;Park, Hye-Won;Lee, Seung-Cheol
    • Korean Journal of Food Science and Technology
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    • v.52 no.2
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    • pp.200-203
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    • 2020
  • The effect of stigmasterol (SS), a phytosterol, in the liposome bilayer on the stability of encapsulated ascorbic acid (ASA) was evaluated. Liposomes, consisting of phosphatidylcholine (PC) and SS, and ASA were encapsulated by the dehydration/rehydration method. The average particle size of the liposome increased with increasing SS content. SS significantly increased the stability of encapsulated ASA. For example, ASA remaining in the liposomes of 100:0, 90:10, and 70:30 (PC:SS, w/w) ratios was 34.12%, 49.88%, and 58.58%, respectively, after storage for 8 days at 4℃, while only 7.66% ASA remained in the buffer under the same conditions. These results indicated that SS in liposomes increased the stability of encapsulated ASA.

Performance Evaluation of Flash Memory-Based File Storages: NAND vs. NOR (플래시 메모리 기반의 파일 저장 장치에 대한 성능분석)

  • Sung, Min-Young
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.9 no.3
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    • pp.710-716
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    • 2008
  • This paper covers the performance evaluation of two flash memory-based file storages, NAND and NOR, which are the major flash types. To evaluate their performances, we set up separate file storages for the two types of flash memories on a PocketPC-based experimental platform. Using the platform, we measured and compared the I/O throughputs in terms of buffer size, amount of used space, and kernel-level write caching. According to the results from our experiments, the overall performance of the NAND-based storage is higher than that of NOR by up to 4.8 and 5.7 times in write and read throughputs, respectively. The experimental results show the relative strengths and weaknesses of the two schemes and provide insights which we believe assist in the design of flash memory-based file storages.

Flash Translation Layer for the Multi-channel and Multi-way Solid State Disk (다중-채널 및 다중-웨이반도체 디스크를 위한 플래시 변환 계층)

  • Park, Hyun-Chul;Shin, Dong-Kun
    • Journal of KIISE:Computing Practices and Letters
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    • v.15 no.9
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    • pp.685-689
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    • 2009
  • Flash memory has several features such as low~power consumption and fast access so that there has been various research on using flash memory as new storage. Especially the Solid State Disk which is composed of flash memory chips has recently replaced the hard disk. At present, SSD adopts the multi-channel and multi-way architecture to exploit advantages of parallel access. In this architecture, data are written on SSD in a unit of a superblock which is composed of multiple blocks in which some blocks are put together. This paper proposes two schemes of selecting, segmenting and re-composing victim superblocks to optimize concurrent processing when a buffer flush occurs. The experimental results show that 35% of superblock- based write operations is reduced by selecting victims and additional 9% by composition of superblock.

An Efficient Overlay Multi-cast Scheduling for Next Generation Internet VOD Service (차세대 인터넷 VOD 서비스를 위한 효율적인 오버레이 멀티캐스트 스케줄링)

  • Choi, Sung-Wook
    • Journal of the Korea Computer Industry Society
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    • v.9 no.2
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    • pp.53-62
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    • 2008
  • Intensive studies have been made in the area of IPTV VOD server. The basic goal of the study is to find an efficient mechanism to allow maximum number of users under the limited resources such as Buffer utilization, disk performance and network bandwidth. The overlay multicast that has been recently presented as an alternative for the IP multicast has been getting much persuasion by the system resource and the network bandwidth and the advancement of the network cost. we propose a efficient overlay multi_casting network policy for multimedia services with multi media partition storage. Simulation results show that the rate of service number and service time of proposed scheme are about 23% performance improved than that of traditional methods. This implies that our method can allow much more users for given resources.

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Isolation and Properties of $\beta$-N-Acetyl-D-glucosaminidase B from Rat Uterus

  • Jung, Jin-Ha;Yang, Chul-Hak
    • Bulletin of the Korean Chemical Society
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    • v.4 no.3
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    • pp.139-143
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    • 1983
  • ${\beta}$-N-Acetyl-D-glucosaminidase B was highly purified with the following sequence of steps; DEAE-cellulose, CM-cellulose, and Sephadex G-200 gel filtration chromatograpies. The specific activity of the purified ${\beta}$ -N-acetyl-D-glucosaminidase B was 2.2 units/mg protein with 12.9 % yield and 196.2 fold purity. The purified ${\beta}$-N-acetyl-D-glucosaminidase B showed single band on polyacrylamide gel electrophoresis. The final preparation of ${\beta}$ -N-acetyl-D-glucosaminidase B was completely free friom arylsulfatase and ${\beta}$-glucuronidase. ${\beta}$ -N-Acetyl-D-glucosaminidase B had pH optimum of 4.5 in 0.5 M sodium citrate buffer. The molecular weight of ${\beta}$-N-acetyl-D-glucosaminidase B was 133,000 by Sephadex G-200 gel filtration. The Km value of ${\beta}$-N-acetyl-D-glucosaminidase B using p-nitrophenyl-N-acetyl-${\beta}$-D-glucosaminide as substrate was 1.0 mM and $V_{max}$ was 0.014 ${\mu}$ mole/min. ${\beta}$-N-Acetyl-D-glucosaminidase B was stable at $55^{circ}C$ for 70 minutes. The crude ${\beta}$ -N-acetyl-D-glucosamiinidase in 70 % ammonium sulfate retained 93 % activity after 7 months storage at -$55^{circ}C$. Bovine serum albumin, sodium chloride, and phosphate activated ${\beta}$ -N-Acetyl-D-glucosaminidase B. N-Acetyl-D-glucosamine, ${\alpha}$-methyl-D-mannoside, and acetate inhibited ${\beta}$ -N-acetyl-D-glucosaminidase B.

Metal Vapor Laser Research II. (금속증기레이저 연구 II)

  • 이재경;정환재;임기건;이형종;정창섭;김진승
    • Korean Journal of Optics and Photonics
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    • v.3 no.3
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    • pp.178-182
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    • 1992
  • An air-cooled discharge-heated copper-vapor laser system with its inter-electrode distance of 45 cm has been developed by utilizing an alumina ceramic plasma tube of 1.6 cm in diameter and 50 cm in lengih. For operating the laser, a dc high voltage power supply with output rating of 6 kV and 500 mA, a resonant charging circuitry consisting partly of an 1.8 H inductor assembly and a 5 nF storage capacitor, and a thyratron driver operating up to 7 kHz have also been developed. The present laser system starts lasing at the tube temperature of about $1350^{\circ}C$ and an maximum average output power of 0.7 W has been obtained at 12 kV, 4.5 kHz. 50 mbar of Ne buffer gas pressure, and at the tube temperature of $1460^{\circ}C$.

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