• Bulletin of the Korean Chemical Society
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• v.20 no.12
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• pp.1464-1468
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• 1999

Non-activated indolinobenzospiropyrans, 6-(p-substituted phenylazo)-1',3',3'-trimethylspiroindolinobenzopyrans(1-4) have been synthesized by the reaction of commercially available Fischer's base with 2-hydroxy-5-arylazobenzaldehyde (S1-S4). The arylazosalicylaldehydes were obtained from the diazocoupling reaction of substituted anilines with salicylaldehyde. The rate of spiro-ring formation from the open form of these nonactivated spiropyran derivatives at room temperature has been investigated utilizing the stopped-flow method de-veloped earlier. Half life times $(t_{1/2})$ of the ring-closure reaction in ethanol are about 0.3-14 seconds for the nonactivated spiropyrans examined. UV-Visible absorption spectral data of the open merocyanine form of nonactivated spiropyrans, which showed no chromotropism at room temperature, have also been obtained.

• Journal of Korea Water Resources Association
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• v.30 no.6
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• pp.777-785
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• 1997

This study was conducted to clarify the hydrological characteristics of subsurface flow through a soil matrix and macropores. The research facility was set up in a 20m-1ong trench excavated down to bedrock at the base of a hillslope in the Panola catchment under USGS Georgia district. 13 macropores were found on the trench face and 6 major macropores were monitored. Matrix and macropore flow were measured during 95.5mm rainfall on March, 6 to 7. 1996. Macropore flow had great influence on formation of peak flow because the delivery time to Peak flow of macropore flow were faster about 10hrs than those of matrix flow. Matrix flow continued to recess for 3 days. On the other hand, macropore flow stopped within 12hrs after the event ceased. This means that matrix flow controls the recession part. The spatial variations of matrix and macropore flow between each trough and collector were very large by a wide range of 8,655.3 $\ell$ to 17.8 $\ell$ . The bed rock surface topography relates closer with the spatial variations of the flow than the surface one.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.10
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• pp.4005-4011
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• 2010

This study investigates the effect on automobile traffic flow of the bike lane configuration via the road diet implemented in Nam-Gu, Busan using the microscopic simulator TransModeler. The results show that the increases in average delay time and stopped time experienced by automobile vehicles after implementing the road diet during the peak period are significant, but those during the non-peak period are insignificant at alpha=0.1. The results in peak period are consistent with the results of the past studies, which concluded that a road diet can contribute to deteriorating the level of service when the automobile traffic flow rate on the existing roadway is relatively high. For the non-peak period, the analysis results may reflect the fact that the automobile traffic flow is not severly affected by the road diet due to the reserved capacity.

• Journal of Korean Society of Transportation
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• v.27 no.2
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• pp.199-206
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• 2009

In relation with economical efficiency analysis on investment evaluation of transportation system, among vehicle operating cost saving benefit that is applied to general preliminary assessment guidelines and investment evaluation guidelines, oil expense calculated data which concentrated and analyze on the relationship between oil consumption amount on running state and running speed. For uninterrupted flow which does not have stopped delay due to traffic signal, consideration for reduction benefit is possible due to the changes of running speed and travel time however, for interrupted flow which the stopping occurs due to signal control on actual signal intersection has no consideration for stopping delay time reduction and stopping rate improvement thus reflection of reality on improved effect analysis is difficult. Therefore, this research makes a framework to analyze benefits that reflects the features of signalized intersections by benefits associated with decrease of stopping delay time with existing research and developing vehicle operating cost calculation model formula. Vehicle operating cost has been redefined considering the stopping delay time by applying the oil consumption amount at idling and the economical benefit between conventional model and newly developed model when applied for the optimization of traffic signal system on the two roads in Seosan city has been analyzed comparative. While the importance of traffic system maintenance is being emphasized due to the increase of congested areas on roads, it is expected to assist in more realistic economical analysis which reflect the delay improvement through the presentation of an economic analysis model that considers the features of signalized intersections in signal optimization system improvements and effect analysis of congestion improvement projects`.

• Journal of the Korean Chemical Society
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• v.58 no.6
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• pp.560-568
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• 2014

The contribution of electrostatic interactions to protein folding reaction was studied by using mutant ubiquitin with lysine to alanine mutation at residue position 29. Based on the three dimensional structure of ubiquitin, lysine 29 is located close to negatively charged glutamate 16 and aspartate 21 and considered to stabilize the native state of ubiquitin by electrostatic interactions between these residues. The equilibrium unfolding experiment showed that the native stability was decreased by about ~20% upon mutation. This observation indicates lysine 29 indeed forms electrostatic interactions with nearby residues. Folding kinetics measurements using stopped-flow device and quantitative analysis of kinetics data indicate that ubiquitin folds from unfolded state to native state via intermediate state as observed previously. This intermediate state was observed to form immediately after the initiation of folding reaction. The folding intermediate was shown to be destabilized considerably upon lysine to alanine mutation. These observations indicate that electrostatic interactions can form early stage of protein folding and hence lead the folding reaction.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.4
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• pp.743-750
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• 1997

Direct measurement of the kinetics of free fatty acid transfer between phospholipid model membrane is technically limited by the rapid nature of the transfer process. Separation of membrane-bound fatty acid by centrifugation has shown that although the equilibrium distribution of free fatty acid is determined by this method, fatty acid transfer occurs too rapidly for accurate kinetic measurements. Recently fluorescence resonance energy transfer(FRET) assay has been developed to examine transfer of fatty acids between membranes. Donor membranes which has fluorescent fatty acid, anthroyloxy fatty acid(AOFA), is mixed with acceptor membranes which has non-interchangeable fluorescent quencher, nitrobenzo-xadiazol(NBD), using stopped flow apparatus. As the fluorescent fatty acids transfer from donor membrane to acceptor membrane, fluorescence intensity would be decreased and the rate and degree of fatty acid transfer can be analyzed. Fatty acid transfer between micelles is more complicated because of bile salt. Therefore in experiments with micelles, fluorescence self quenching assay is used. At high concentrations, a fluorophore tends to quench its own fluorescence causing a reduction in fluorescence intensity. Donor micelles contained self quenching concentrations of fluorophore and acceptor micelles had no fluorophore. Upon mixing of donor and acceptor micelles, the rate of transfer of the fluorophore from the donor to the acceptor was measured by monitoring the release in self quenching when its concentration in donor decreased over time.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.39 no.1
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• pp.63-71
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• 2019

The two-fluid model proposed by Herman and Prigogine is useful for analyzing macroscopic traffic flow in a network. The two-fluid model is used for analyzing a network through the relationship between the ratio of stopped vehicles and the average moving speed of the network, and the two-fluid model has also been applied in the urban transportation network where many signalized or unsignalized intersections existed. In general, the average travel speed and moving speed of a network decrease, and the ratio of stopped vehicles and low speed vehicles in network increase as the traffic demand increases. This study proposed the two-fluid model considering congested and uncongested traffic situations. The critical velocity and the weight factor for congested situation are calibrated by minimizing the root mean square error (RMSE). The critical speed of the Seoul network was about 34 kph, and the weight factor of the congestion on the network was about 0.61. In the proposed model, $R^2$ increased from 0.78 to 0.99 when compared to the existing model, suggesting that the proposed model can be applied in evaluating network performances or traffic signal operations.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.5
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• pp.1026-1031
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• 2003

Automatic feed pumps are operated and stopped by a pressure switch. Thus, because of repeated operations and stops of the pumps according to fluctuations of water volume, operation with constant rate and pressure is impossible. Moreover, because of repeated running of the pump, keeping up of constant pressure is impossible and damage and energy loss are weak points of the pimps. To make up for defects of an automatic feed pump, this paper designed and made a static pressure feed system which was composed of a feed water control valve, a flow sensor and a control system. The valve device plays an important part in reducing load of pumps by constant water supply regardless of outflow of water. Outflow of water is determined by infrared diode of the flow sensor. The control system is made of a 8 bit micro-processor and the pump was controled by a specific control algorithm. With the constant pressure feed system, discharge pressure was kept at fixed pressure, accurate operations and stops were smoothly accomplished and the pump was operated with constant pressure. Thus, the constant pressure feed system can be considered as an advanced system which made up for the weak points in the current automatic feed systems.

• Journal of the Korean Chemical Society
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• v.45 no.3
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• pp.223-229
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• 2001

A method for the determination of glucose in human whole blood by chemiluminescence method using a stopped flow injection system has been studied. The method is based on the differences in the chemiluminescence intensities of luminol due to the different amounts of hydrogen peroxide produced from the glucose oxidase catalyzed reaction. The enzyme reactor was prepared by immobilization of glucose oxidase on aminopropyl glass beads and the chemiluminescence from a flow cell was measured by means of an optical fiber bundle. In order to obtain the optimum experimental conditions, effects of pH for the chemiluminogenic solution and enzyme reactor, flow rate and temperature on the chemiluminescence intensity were investigated. The calibration curve obtained under optimum experimental conditions was linear over the range from $1.0{\times}10^{-1}$ mM to 7.0 mM and the detection limit was $6.0{\times}10^{-2}$ mM. The proposed method was applied to the determination of glucose in whole human blood sample and the results were compared with those obtained by an official method. The present method was also evaluated by the results of recovery experiments.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.1
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• pp.47-58
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• 1994

Field observation and numerical experiments with a two-dimensional depth-integrated model were undertaken in order to investigate some of the effects on the flow structure resulting from the construction of a bridge connecting Kohung Peninsula and the Narodo Islands on the southern coast of Korea. Tidal currents passing through the straits between the Narodo Islands showed that, although the phase lagged one hour behind that passing through the strait between Kohung Peninsula and Naenarodo Island, it still kept strong flows of more than 80cm/sec near the bottom. The seawater temperature and salinity within the study area seemed to be higher southward but uniform vertically. The results of the drogue experiments in the straits between the Narodo Islands showed that the drogues moved northward of Sayangdo in the early part of the flood tide, but southward in the late part of the flood tide and finally stopped a mile from the east coast of Surakdo. On the other hand, the numerical computation showed that the flow structures after construction of the bridge piers were basically in line with those before construction of the bridge piers, except for the slight variations of velocities in the vicinity of the bridge piers. A large scale clockwise circulation has been confirmed in the south area of Namsungri of Kohung Peninsula from the computational results of tide-induced residual currents. Referring to these computational results, the impact category on the flow structures due to the bridge piers construction has been estimated to be within around 2km. The results were in good agreement with the field observations.