• Proceedings of the Botanical Society of Korea Conference
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• 1995.07a
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• pp.133-170
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• 1995

The major objectives of fruit breeding lie in improvement of cultivar, easy to be cultivated and of high quality, in order to produce unexpensive, delicious fruit both for fresh fruit market and processing. Recently, fruit breeding in Korea has contributed to breeding of several superior cultivars in major fruit crops, resulting in appreciable improvement in qualities such as skin color, taste and fruit-bearing habit concerned with productivity. In spite of accomplishments mentioned above, the need for both highly disease-resistant cultivars and long-keeping, physiological disorder-resistant cultivars to meet long distance transsportation in the temperate fruit crops of apples, oriental pears, stone fruits such as peaches, and grapes grown in Korea is rapidly pressing more than ever, as cultivars of today susceptible to pests and diseases and vulnerable to physiological disorders are very expensive and time-consuming in post-harvest handling and management. Thus, imporvements made in the above problems through breeding level will lead to the really enhanced productivity in fruit industry. The major impediments of tree size, the long length of juvenile period and the highly heterogeneous genetic composition to the improvement of fruit crops are responsible for the lower amount and rate of improvements of fruit crops as compared to annuals. Considering the expected limitations of the above problems to be solved through conventional breeding methods and strategy, a turning point of breeding a near perfect cultivar would be laid down if innovative breakthroughs in biological technology will be realized in applying some of the techniques of genetic manipulation at the molecular level to the cultivar improvement of fruit crops, such as the selective insertion of DNA carrying genes that govern desirable characteristics. More than anything else, those traits such as fruiting habit deciding productivity, elements of fruit qualities conditioned by monogene, and disease-and pest-resistance of vital importance for successful fruit growing are urgently desired to be improved by advancement of biotechnology for they are more than difficult and need long period to be attained through conventional breeding method.

• Horticultural Science & Technology
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• v.33 no.3
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• pp.317-325
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• 2015

Peach (Prunus persica L.) is a model species for stone fruit studies within the Rosaceae family. Auxin plays an important role in the development of peach fruit. To reveal the distribution of auxin in the tissues of peach fruit, immunohistochemical localization of IAA was carried out in the seed, mesocarp, and endocarp in developing peach fruit using an anti-indole-3-acetic acid (anti-IAA) monoclonal antibody. A strong IAA signal was observed throughout the outer and inner integument during peach fruit development, and the distribution was zonal. The IAA signal was mainly focused in mucilage layers in the outer integument. The outer integument may function to produce or store IAA in the seed; a strong IAA signal was detected in the cells around the vascular tissue, whereas a weak IAA signal was located in the vascular tissues. In the mesocarp, the cells around the vascular bundle tissue gave rise to an IAA signal that increased in the late phase of fruit growth, which coincided with a significant increase in fruit growth. The distribution of IAA, however, was changed when fruit was treated with auxin transport inhibitors NPA (1-N-naphthylphthalamic acid) or TIBA (2, 3, 5-triiodobenzoic acid); in mesocarp tissues, an IAA signal was detected mainly in vessels of the treated fruit. During the critical period of endocarp lignification, the vessel lignification process was negatively correlated with IAA signal. The present results confirmed that the distribution of IAA was different in various tissues of peach fruit according to the developmental stage. This research provides cytological data for further study of the regulatory mechanism of auxin in peach fruit.

• Korean journal of applied entomology
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• v.58 no.4
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• pp.313-317
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• 2019

Stink bugs (Hemiptera: Pentatomidae) are economically important pests of fruit trees in Korea. The aim of the present study was to survey the relative abundance of stink bugs on four stone fruits, maesil (Prunus mume Sieb. et Zucc), cherry (Prunus avium L.), plum (Prunus salicina Lindl), and peach (Prunus persica (L.) Batsch), from 2017 to 2019 in the field. Four stink bug species were observed, including Carbula putoni (Jakovlev), Dolycoris baccarum (L.), Halyomorpha halys (Stål), Plautia stali Scott. H. halys was the most abundant (65%), followed by P. stali (26%), C. putoni (7%), and D. baccarum (2%). H. halys was the dominant species in maesil, cherry, and peach fruits, whereas P. stali was the dominant species in plum fruits. Most (81%) of the stink bugs observed were adults, with nymphs accounting for only 19% of the observed specimens. More stink bugs were observed during the late season than during the early season. These finding suggest that control strategies should be developed for the management of H. halys and P. stali at harvest in stone fruit tree orchards in Korea.

• Journal of Practical Agriculture & Fisheries Research
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• v.15 no.1
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• pp.95-105
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• 2013

This study has been conducted to investigate the effect of Urea and K₂SO₄ treatment at stone hardening stage and 20 days before harvest on soil chemical properties, mineral nutrient concentration and quality of 'Mibaekdo' fruit peach. K concentration after Urea and K₂SO₄ treatment in soil was increased significantly by Urea 162g+K₂SO₄ 188g/tree(standard amount) treatment at stone hardening stage, K₂SO₄ 1.0% tree-spray, Urea 81g+K₂SO₄ 94g/tree(half amount), Urea 162g+K₂SO₄ 188g/tree and Urea 324g+K₂SO₄ 376g/tree(double amount) soil treatment before harvest 20 days compared to control. T-N, K and Ca concentration in leaf was increased significantly by all treatment. but Na concentration in leaf was increased by Urea 0.5% and K₂SO₄ 1.0% tree-spray treatment before harvest 20 days. T-N concentration in fruit skin was increased significantly by standard amount soil treatment, which decreased by K₂SO₄ 1.0% tree-spray and half amount soil treatment. T-N, K and Ca concentration in fruit flesh(1~10mm depth flesh from peel) were increased markedly by all treatment excepted Urea 0.5% tree-spray. The leaf weight at harvest was increased markedly by Urea 0.5% tree-spray, standard amount and double amount treatment before harvest 20 days. Fruit weight was increased significantly by standard amount compared to all treatment. Red fruit skin(Hunter a value) progress was effective by K₂SO₄ tree-spray, half amount and double amount treatment before harvest 20 days. Fruit SSC was increased significantly by Urea 0.5% and K₂SO₄ tree-spray before harvest 20 days, standard amount treatment at stone hardening stage compared to control.

• Korean Journal of Organic Agriculture
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• v.23 no.2
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• pp.291-299
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• 2015

This study aimed to investigate the effect of inoculation time of phosphate- solubilizing Kluyvera sp. CL2 on fruit quality in cultivation of Campbell early' grape. When phosphate-solubilizing strain was treated at the stone-hardening stage, soil phosphorous increased, exchangeable cations such as K, Ca and Mg also increased. Soil pH was not changed severely due to the soil buffer capacity. Water soluble phosphate concentrations did not decrease heavily up to 20 days after inoculation. When this strain was treated at the berry-softening stage, soil phosphate solubilization ratio was high, cluster weight and sugar content also increased. Both anthocyanin contents and Hunter's values were seen to be significant when inoculation times were stone-hardening stage and berry-softening stage, in particular, increase of Hunter's value a resulted in the improvement of coloration. From these results, we could find that the inoculation of phosphate-solubilizing Kluyvera sp. CL2 at berry-softening stage was the most effective in improvement of fruit productivity and quality in cultivation of'Campbell early' grape.

• Natural Product Sciences
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• v.13 no.2
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• pp.97-100
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• 2007

The present study includes macro and microscopical details, powder study, physico-chemical study and HPTLC fingerprinting of the Piper longum fruits. Microscopic studies revealed the presence of stone cells, starch grains and thin walled fragments of parenchymatous cells. Physico-chemical studies showed alcohol and ether soluble extract 24.53 and 6.7, sugar 0.35, starch 21.33 and tannin 0.83% respectively. Successive soxhlet extract showed maximum percentage of hexane soluble fraction i.e. 22.52. The HPTLC profile has also been performed against the reference marker pipeline, which was identified at R$_f$ 0.42. In the present paper a detailed pharmacognostical evaluation of fruit has been undertaken.

• Horticultural Science & Technology
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• v.32 no.1
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• pp.33-40
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• 2014

This study was carried out to understand the physiological characteristics of early-matured 'Hanareum' (Pyrus pyrifolia Nakai) pears through anatomical structure and fruit characteristics and also the changes according to gibberellin (GA) treatment. The pericarp at full bloom consists of outer epidermis, hypodermis, parenchyma cell, and inner epidermis from the exterior and five types of vascular bundle tissues. Cork cell layer was formed at 70 days after full bloom (DAFB) in non-treated fruits and formed at 60 DAFB in GA treated fruits. Cell division period was from full bloom (FB) to 40 DAFB and then fruit enlargement was accomplished by the cell growth. Comparison of the fruit enlargement and fruit structure development by GA treatment or non-treatment showed that cell division of 'Hanaerum' fruits did not affect the GA treatment but fruit enlargement was affected cell growth. Fruit stalk of GA treatment fruits was larger than non-treated fruits from 40 DAFB which correspond to the period of the stop of cell division and 'Hanareum' was regarded GA treatment expedite of vascular bundle tissue growth and relatively increased nutrient transport to fruit. In addition to, average fruit quality between the non-treatment and GA treatment showed that fruit weight was higher in fruits treated by GA but firmness was lower and probably was effected fruit storing in 'Hanareum' pear.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.959-965
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• 2016

In 1994, a new cultivar 'Joyskin' was created from a cross between the cultivars 'Whangkeumbae' and 'Waseaka' at the Pear Research Institute of the National Institute of Horticultural and Herbal Science, Rural Development Administration. In 2006, the 'Joyskin' was selected from among the 317 seedlings resulting from the cross for its skin and taste qualities. Regional adaptation tests were conducted in nine regions and in ten experimental plots from 2006 to 2011. The cultivar was named in 2011. 'Joyskin' showed a vigorous growth habit and semi-spread characteristics similar to 'Whangkeumbae'. The average full bloom date for 'Joyskin' was April 21st, which was also similar to 'Whangkeumbae'. The optimum fruit ripening time was September 6-8th, which was six or eight days earlier than 'Whangkeumbae'. The fruit was round in shape and the skin was a golden yellow color at maturity. The average fruit weight was 320 g and the flesh firmness was $2.5kg/8mm{\varphi}$. The firmness of the fruit skin determined by a blade-type plunger of texture analyzer was 22.9 N, which was significantly different from that of 'Whangkeumbae' 29.9N. Stone cell analysis of 'Joyskin' by phloroglucinol-HCl, showed that 'Joyskin' stone cells were small in size and few in numbers cpmpared to those of cultivars of was 'Manpungbae', 'Niitaka', and 'Whangkeumbae'. The patent application for 'Joyskin' was submitted in April, 2012 (Grant No. 2012-337). In 2016, 'Joyskin' (Grant No. 5895) was registered as a separate record, with uniformity and stability per Korean Seed Industry Law.

• Korean Journal of Environmental Agriculture
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• v.29 no.4
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• pp.367-373
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• 2010

The study was established to compare fruit qualities and antioxidant compounds in 'Niitaka' pear (Pyrus pyriforia) trees grown in the organic and conventional farming systems. Fruits in the organic system appeared to have dark red color on the fruit surface. Fruit weight, soluble solids, acidity, firmness, and stone cells were not different between the farming systems. Organic fruits had a greater potassium concentration than the conventional fruits, but phosphorous, calcium, and magnesium concentrations in fruits were not different between the treatments. Peel, flesh, and juice parts in the organic fruits had greater phenolic compounds compared to the conventional fruits. Peel parts had much greater antioxidant compounds than the flesh parts, regardless of the treated-fruits. All fruits grown in the conventional and organic systems had a similar DPPH ($\alpha$, $\alpha$-diphenyl-$\beta$-picryl-hydrazyl) radical-scavenging activity in the peel, but flesh parts in organic fruits had a greater DPPH than the conventional fruits. Phenol and flavonoid compounds in the peel and flesh were positively related to the DPPH radical-scavenging activity. There were no significant differences for the nitrite scavenging activity in the peel and flesh parts between the treatments.

• The Plant Pathology Journal
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• v.31 no.1
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• pp.41-49
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• 2015

Sweet cherry is an important fruit crop with increasing economical value in Turkey and the world. A number of viruses cause diseases and economical losses in sweet cherry. Prune dwarf virus (PDV), is one of the most common viruses of stone fruits including sweet cherry in the world. In this study, PDV was detected from 316 of 521 sweet cherry samples collected from 142 orchards in 10 districts of Isparta province of Turkey by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). The presence of PDV in ELISA positive samples was confirmed in 37 isolates by reverse transcription- polymerase chain reaction (RT-PCR) method. A genomic region of 862 bp containing the coat protein (CP) gene of PDV was re-amplified from 21 selected isolates by RT-PCR. Amplified DNA fragments of these isolates were purified and sequenced for molecular characterization and determining genetic diversity of PDV. Sequence comparisons showed 84-99% to 81-100% sequence identity at nucleotide and amino acid level, respectively, of the CP genes of PDV isolates from Isparta and other parts of the world. Phylogenetic analyses of the CP genes of PDV isolates from different geographical origins and diverse hosts revealed that PDV isolates formed different phylogenetic groups. While isolates were not grouped solely based on their geographical origins or hosts, some association between phylogenetic groups and geographical origins or hosts were observed.