• The Plant Pathology Journal
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• 제31권4호
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• pp.343-349
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• 2015

Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is one of the most important bacterial diseases of citrus. Because citrus canker is not found in many countries including European Union and Australia, Xcc is strictly regulated in order to prevent its spread. In this study, the effects of X-irradiation on Xcc growth either in the suspension or on the surface of citrus fruits were investigated. The suspension containing $1{\times}10^7cfu/ml$ of Xcc was irradiated with different absorbed doses of X-irradiation ranging from 50 to 400 Gy. The results showed that Xcc was fully dead at 400 Gy of X-irradiation. To determine the effect of X-irradiation on quarantine, the Xcc-inoculated citrus fruits were irradiated with different X-ray doses at which Xcc was completely inhibited by an irradiation dose of 250 Gy. The $D_{10}$ value for Xcc on citrus fruits was found to be 97 Gy, indicating the possibility of direct application on citrus quarantine without any side sterilizer. Beside, presence of Xcc on the surface of asymptomatic citrus fruits obtained from citrus canker-infected orchards was noted. It indicated that the exporting citrus fruits need any treatment so that Xcc on the citrus fruits should be completely eliminated. Based on these results, ionizing radiation can be considered as an alternative method of eradicating Xcc for export of citrus fruits.

• 한국도시환경학회지
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• 제18권4호
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• pp.439-453
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• 2018

본 연구에서는 자연공원내 주요 음용수에 대한 수질특성을 평가하여 기초정보를 제공함과 적절한 관리방안을 제언하는데 그 목적이 있다. 분석결과 미생물은 자연공원이라는 지리적 특성상 대부분 검출되는 것으로 나타났고, 일부 대상지에서는 현재 자외선살균기 및 오존처리를 시행하고 있듯이 기타 주요 음용수에도 확대하여 사용하는 것이 적절한 것으로 판단된다. 또한 가정에서 음용시 끓여 마심으로 자연소독을 하는 것이 적절한 것으로 판단되며, 탁도 등이 일부 먹는 물 수질기준(샘물)을 초과하는 것으로 나타났으나, 음용수 주변의 환경정화 등으로 개선할 수 있는 것으로 판단된다. 전반적으로 자연공원내 주요 음용수는 먹는물 수질기준(샘물)에 적합한 것으로 나타났고, 청소 등의 정기적인 관리와 개선이 필요한 것으로 판단된다.

• 한국응급구조학회지
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• 제23권1호
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• pp.61-69
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• 2019

Purpose: The study aims to provide basic data to enhance the health of paramedics responsible for patient transport and treatment by analyzing the exposure level of paramedics to electromagnetic waves generated by electric devices used in ambulances. Methods: The study measured electromagnetic waves in ambulances in N region from July to December 2018. ME3030B produced by German Gigahertz Solutions was used to measure these waves and the maximum value was selected by moving it slowly in various directions. Each measurement part was selected and the mean value was calculated by repeatedly measuring at 10-minute intervals three times in total: $1^{st}$, $2^{nd}$, and $3^{rd}$ phase. Results: Among the electrical devices installed in the patient room of the ambulance measured at distances of 1 cm or 30 cm, results showed a high level of electric waves at the inverter ($26.25{\pm}39V/m$) and high level of electromagnetic waves ($564.00{\pm}31.75nT$) at the ozone sterilizer. According to measurements toward the front near the driver's seat, results indicated high levels of electric waves ($3.67{\pm}1.15V/m$) and electromagnetic waves ($450.00{\pm}19.52nT$) at the black box hard drive. Conclusion: Electromagnetic waves within the ambulance were stable and not beyond the range that might impact human health. However, in the case of the black box hard drive ($3.67{\pm}1.15V/m$, $450.00{\pm}19.52nT$) located under the passenger seat, it may have a direct effect on the human body and, thus it is necessary to move it to a storage area further away from the paramedics to minimized the impact.

• 교육녹색환경연구
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• 제18권1호
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• pp.38-48
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• 2019

본 연구는 대학 연구실 사고 예방을 위해서 실험 전 이루어지는 형식적인 일상점검을 보다 실효성 있는 일상점검표를 제시하는데 목적이 있다. 이를 위해 현재 실시중인 일상점검표와 선행연구자료를 재구성하여, 이를 토대로 2차에 걸친 전문가 델파이 조사로 연구를 실시하였다. 그 결과 연구실 정리정돈 등 일반안전 4개, 기계 및 공구 조임부 이상여부 등 기계기구안전 3개, 전기분전반 주변 적재금지 등 전기안전 3개, 유해인자취급 및 관리대장 비치 등 화공안전 6개, 소화기 점검 등 소방안전 3개, 가스용기 점점 등 가스안전 5개, 손 소독기 관리상태 등 생물안전 1개, 기타 1개, 총 26개 항목을 일상점검표로 제시하였다. 관련 전문가들의 의견을 종합해 보면, 실질적인 일상점검을 위해서는 쉽고 간소화 된 일상점검표가 필요한 것으로 나타났다.

• 급식외식위생학회지
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• 제2권2호
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• pp.59-66
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• 2021

The results of the inspection based on the sanitation checklist and the measurement of refrigerator/freezer temperature, illuminance, and ATP were conducted at 12 school foodservices in Daiyue-district, Taian-city, Shandong Province, China, at the same time, and the results were as follows. Five items of 'Use sterilized cutting boards', 'Use sterilized knives', 'Use worktable after sterilization', 'Use sink after sterilization', 'Sterilize the interior/exterior of the refrigerator regularly' in the checklist inspection were found not to be performed (0%). Two items of 'Disinfect hands after washing', 'Equipped with hand sterilizer' and 'Sterilize the kitchen areas regularly' were investigated as being performed only in one foodservice (8%). The average illuminance of the receiving stand were 373.08±106.35 Lux, 8% when the standard (540 Lux or higher) was complied with, the average refrigerator temperature were 7.06±0.82℃, 38.9% when the standard (-2 to 5℃) was observed 8% of cases complying with the standard (below -18℃) at -8.56±0.76℃ were investigated. As a result of ATP measurement, the surfaces of knife edge, cutting board, refrigerator door handle, worktable, and sink, which are not sterilized at all, were not suitable (accept ratio 0%), sterilized food plate (accept ratio 54.2%) and utensil for preserved food (accept ratio 75%) had a relatively high accept ratio. China's hygiene regulations should be revised in the direction of strengthening disinfection practices for facilities, equipment, and the environment, and hygiene measures such as education on disinfection and preparation of economical disinfection methods should be established.

• Biomolecules & Therapeutics
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• 제30권2호
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• pp.126-136
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• 2022

Liver fibrosis is part of the wound healing process to help the liver recover from the injuries caused by various liver-damaging insults. However, liver fibrosis often progresses to life-threatening cirrhosis and hepatocellular carcinoma. To overcome the limitations of current in vivo liver fibrosis models for studying the pathophysiology of liver fibrosis and establishing effective treatment strategies, we developed a new mouse model of liver fibrosis using polyhexamethylene guanidine phosphate (PHMG-p), a humidifier sterilizer known to induce lung fibrosis in humans. Male C57/BL6 mice were intraperitoneally injected with PHMG-p (0.03% and 0.1%) twice a week for 5 weeks. Subsequently, liver tissues were examined histologically and RNA-sequencing was performed to evaluate the expression of key genes and pathways affected by PHMG-p. PHMG-p injection resulted in body weight loss of ~15% and worsening of physical condition. Necropsy revealed diffuse fibrotic lesions in the liver with no effect on the lungs. Histology, collagen staining, immunohistochemistry for smooth muscle actin and collagen, and polymerase chain reaction analysis of fibrotic genes revealed that PHMG-p induced liver fibrosis in the peri-central, peri-portal, and capsule regions. RNA-sequencing revealed that PHMG-p affected several pathways associated with human liver fibrosis, especially with upregulation of lumican and IRAK3, and downregulation of GSTp1 and GSTp2, which are closely involved in liver fibrosis pathogenesis. Collectively we demonstrated that the PHMG-p-induced liver fibrosis model can be employed to study human liver fibrosis.

• 한국치위생학회지
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• 제11권6호
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• pp.973-983
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• 2011

Objectives : The oral care during early childhood plays an important role to maintain sound oral health during adulthood. As the number of children's using child care facilities is on the rise recently, the children's oral health awareness and behavior of the educators at nurseries and kindergartens are to be examined. Methods : A self-recording survey was conducted on 194 educators at the nurseries and kindergartens in Chungnam region. Results : The most frequently given snack was milk, 91.8 percent of the facilities had children brush their teeth, 63.4 percent made them apply fluoride and 56.2 percent kept children's toothbrushes in an ultraviolet rays sterilizer. There was a meaningful difference in the awareness of the necessity to educate children about oral health according to the offer of the opportunity to apply fluoride (p<0.05). A meaningful difference was found in the recognition of the necessity to educate children according to their grade (p<0.05) and to train teachers themselves (p<0.05). The most desirable persons in charge of oral health education were dentists in health centers (46.9%) in order. The more experience in teaching they have had (p<0.05), when they're married (50.5%) (p<0.05) and when they're not homeroom teachers (52.6 percent), the more regular checkups they have had (p<0.05). As for the importance of oral health and the results of regular checkups, those who had answered 'very important'(42.4%) showed higher rate of regular checkup (p<0.05) than those who had answered 'important'(23.9%). When teachers have the experience to get trained about oral care (96.3%), the practice frequency of brushing teeth was proved to become higher (p<0.05). Conclusions : The educators for children should recognize the importance of oral health education, educate children to practice oral health care, and the environment and systematic foundation should be established which the educators manage effectively.

• 한국산학기술학회논문지
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• 제13권11호
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• pp.5247-5253
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• 2012

본 연구는 식혜와 액상 커피의 제조 공정중 HACCP system을 이용하여 한계 기준 설정을 위한 목적으로 실시하였다. 살균공정의 한계 기준 설정은 충북 진천시 소재의 P사에서 약 30일 (2012년 4월 1~30일) 동안 살균온도와 시간을 측정하였다. 그 결과, 멸균전에는 식혜와 액상 커피에서 미생물이 검출되었다. 반면에 식혜(238mL Can, 500mL and 300mL PP, 1.8L PP)에서 모든 미생물은 살균($121{\pm}1^{\circ}C$에서 $15{\pm}1$, $35{\pm}1$ and $45{\pm}1$분) 후에는 검출되지 않았고, 액상 커피도 살균($121{\pm}1^{\circ}C$, $20{\pm}1$분)후 검출되지 않았다. 가장 적당한 온도와 시간을 결정하기 위한 살균기 조건은 $121{\pm}1^{\circ}C$, $20{\pm}1$분이었다. 결론적으로, 살균공정은 유해미생물(일반세균, 대장균군, 병원성 미생물)를 예방, 감소 또는 제거할 수 있는 좋은 대안이 될 것이다. 따라서 품질 유지와 생물학적 안전성을 위한 살균 온도와 시간의 한계기준은$121{\pm}1^{\circ}C$에서 $20{\pm}1$분으로 설정하였다. 그리고 HACCP 계획은 살균 공정중 모니터링 방법과 모니터링 주기, 문제 해결 방법, 교육, 훈련, 기록 관리 등을 위하여 필요하여 이를 제안하고자 한다.

• Journal of Nutrition and Health
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• 제49권1호
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• pp.43-50
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• 2016

병원급식소의 HACCP 선행요건 수행 실태를 분석하여 병원급식소 특성을 고려한 선행요건관리 개선 방안을 마련하고자 종합병원 규모의 병원 급식소 총 65곳의 급식관리자를 대상으로 설문조사를 실시한 결과, 선행요건 7개 관리영역 중 영업장관리 영역의 수행도는 선행요건 평가판정그룹별로 유의적인 차이가 있었으며, 적합 그룹 (85점 이상)에서 유의적으로 가장 높았다 (p < 0.001). 위생관리 (p < 0.001), 제조 가공 조리 시설 설비관리 (p < 0.001), 용수관리 (p < 0.001), 보관 운송관리 (p < 0.05) 영역의 수행도는 '부적합' 그룹 (70점 미만)이 '적합'이나 '보완' 그룹 (71점 이상 84점 이하)에 비해 유의적으로 낮았다. 조사대상 병원급식소와 영양사의 일반 특성에 따른 선행요건관리 평가 결과에 대한 차이 분석 결과 서울 경인지역이 대구 경북지역에 비해 선행요건 관리가 '적합'으로 평가된 비율이 유의적으로 높았다 (p < 0.01). 또한 선행요건 판정 그룹에 따른 급식시설 구비율은 '적합' 그룹이 '부적합' 그룹에 비해 3조 싱크 (p < 0.01), 대용량 오븐 (p < 0.01), 대형 에어컨 (p < 0.01), 보온고 (p < 0.01), 보냉고 (p < 0.05), 해동전용 냉장고 (p < 0.05), 위생장화 소독고 (p < 0.05) 등 총 7개 항목의 구비율이 유의적으로 높았다. 병원 급식품질 개선을 위해 HACCP을 빠른 시일 내에 효과적으로 적용하기 위해서는 선행요건 관리 항목이 준수되어야 하며, 이에 앞서 병원 급식소의 운영 특성을 고려한 선행요건관리 기준과 HACCP 관리 계획의 수립이 필요하다고 판단된다.

• 한국임상수의학회지
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• 제22권4호
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• pp.377-381
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• 2005

We tried to extract bone morphogenetic protein (BMP) from the freeze-dried bovine cortical bone (FBCB) for bone graft, which were defatted with chloroform-methanol for 20 days, freeze-dried at $-80^{\circ}C$ for 7 days and sterilized by ethylene oxide gas. Two kg of FBCB were pulverized in a wheel mill to $0.5-2.0mm^3$ cubic in size. The bone particles were demineralized in 0.6N HCI for 10 days at chloroform-methanol$4^{\circ}C$ and defatted with chloroform-methanol for 6 hours at room temperature, which was going to be defatting and demineralized cortical bone (DDM). For extracting BMP, DDM was agitated continuously through 72 hours with magnetic stirrer at $4^{\circ}C$ into 12 times of volume of 6 M guanidine hydrochloride (Gdn-HCl) solution containing proteinase inhibitors to protect BMP such as 2mM N-ethylaleimide, 1mM iodoacetic acid, 1mM phenylmethylsulfonyl fluoride and a sterilizer, 1mM sodium azide. The extraction procedure was repeated for three times. All extracted solution was centrifuged at 10,000 rpm for 30 min and then, the supernatant was dialyzed with 12 times of volume of deionized water at $4^{\circ}C$ for 24-72 hours, which cut off below 6,000-8,000 molecular weight. The dialyzed specimen contained crude-BMP was centrifuged, freeze-dried, and weighted. Through these processing, we could obtained $84.9\%$ as FBCB, $17.8\%$ as DDM and $0.71\%$ as crude-BMP from the wet cortical bone without cancellous bone, marrow and muscles. The crude-BMP were obtained $68.3\%$ from the first extraction, $29.6\%$ from secondary and $2.1\%$ from tertiary, respectively. It was suggested that high yield of crude-BMP migth be explained by three-time repetition of the extraction processing for crude-BMP with Gdn-Hcl sol.