• Journal of Ginseng Research
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• v.26 no.3
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• pp.165-169
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• 2002

Experiments were carried out to select marker for rusty tolerance ginseng root using high rusty degree lines(HRL) and low rusty degree lines (LRL) in ginseng plant. A strong positive correlation was detected between degree of rusty-root in 4-year-root and that in 6-year-root. The contents of phenolic compounds among samples were not different in stele and branch & fine roots. The contents of phenolic compounds of rusty-roots was higher than that of healthy-roots in cortex, but those of high 겨sty degree lines (HRL) were not different compared with low rusty degree lines (LRL) in cortex using same rusty-degree samples. These suggest that phenolic compounds in cortex tissue were not adequate as a marker to select rusty tolerance ginseng roots. The contents of phenolic compounds of rusty-roots were higher than that of healthy-roots in epidermis, and those of HRL were higher than LRL in epidermis using same rusty-degree samples. These suggested that the contents of phenolic compounds in epidermis tissue might be a potent marker to select rusty tolerance ginseng roots.

• Molecules and Cells
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• v.44 no.11
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• pp.830-842
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• 2021

When perceiving microbe-associated molecular patterns (MAMPs) or plant-derived damage-associated molecular patterns (DAMPs), plants alter their root growth and development by displaying a reduction in the root length and the formation of root hairs and lateral roots. The exogenous application of a MAMP peptide, flg22, was shown to affect root growth by suppressing meristem activity. In addition to MAMPs, the DAMP peptide PEP1 suppresses root growth while also promoting root hair formation. However, the question of whether and how these elicitor peptides affect the development of the vascular system in the root has not been explored. The cellular receptors of PEP1, PEPR1 and PEPR2 are highly expressed in the root vascular system, while the receptors of flg22 (FLS2) and elf18 (EFR) are not. Consistent with the expression patterns of PEP1 receptors, we found that exogenously applied PEP1 has a strong impact on the division of stele cells, leading to a reduction of these cells. We also observed the alteration in the number and organization of cells that differentiate into xylem vessels. These PEP1-mediated developmental changes appear to be linked to the blockage of symplastic connections triggered by PEP1. PEP1 dramatically disrupts the symplastic movement of free green fluorescence protein (GFP) from phloem sieve elements to neighboring cells in the root meristem, leading to the deposition of a high level of callose between cells. Taken together, our first survey of PEP1-mediated vascular tissue development provides new insights into the PEP1 function as a regulator of cellular reprogramming in the Arabidopsis root vascular system.

• Korean Journal of Heritage: History & Science
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• v.48 no.1
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• pp.62-79
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• 2015

The Stele for State Preceptor Doseon and Seon Master Sumi of Dogapsa Temple in Yeongam was erected in the fourth month of 1653. It was made with stone obtained from Yeosan(present-day Yeosan-myeon, Iksansi), and contains an inscription - engraved in the spring of 1651 - commemorating the two priests. The project to erect the monument was led by eminent monks, including Gakseong and Sucho, with the support of royal patrons and powerful statesmen including Prince Inpyeong(1622~1658). This monument is thought to be the first of its kind to have a capstone in the shape of a 'dragon loop' handle of the kind traditionally attached to the top of a temple bell. Stone stelae with a dragon-loop-shaped capstone continued to be used by the royalty and nobility of Joseon until the 18th century. The inscription engraved on the rear face of the monument reveals that it was made by Jo Mal-ryong, a renowned stonemason who worked on stone monuments and figures for royal tombs in the mid-Joseon period. He is known as the only stonemason to have been awarded the third highest rank(Jeong-sampum) of government, including the posts of Grand Master(Tongjeong Daebu) and General(Jeolchung Janggun), as recorded in the inscription. The monument corroborates the prevalent view that he was the creator of monumental capstones of the highest aesthetic merit in Joseon, and provides valuable insights about the leading artisans who produced monumental stones for royal tombs in the 17th century. This particular monument is highly regarded as a valuable historical relic because of the detailed information contained in the inscription, including the dates, work processes, patrons and artisans related with its creation, and because it features outstanding workmanship by some of the greatest artisans of the period. The monument also provides important clues about the transition from the Buddhist monuments created under the auspices of the royalty and nobility of mid-period Joseon to the symbolic stone structures and figures made for the tombs of royalty and nobility.

• Journal of Ginseng Research
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• v.24 no.3
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• pp.113-117
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• 2000

To analyze the correlation between the rusty root and the antiokidative activity in ginseng (Panax ginseng C.A.Meyer) roots, the levels of antioxidative activity in various tissues of healthy and rusty roots. The superoxide dismutase activity in rusty roots (126.9 units/mg protein) was approximately 3.5 times higher than that in healthy roots. The catalase activity in rusty roots was approximately 1.6 times higher than that in healthy roots, whereas the peroxidase activity showed a slight low level in msty roots. The 1.1 diphenyl-2-picryl-hydrazyl(DPPH) free radical scavenging activity in rusty roots was approximately 2.0 times higher than that in healthy roots. The total ascorbate content in healthy roots was 166~240 $\mu\textrm{g}$/g fr. wt. depending on the tissues. Interestingly, the oxidized dehydroascorbate (DHA) content occupied more than 80% in total ascorbate content. The total ascorbate content in rusty roots was a similar level with healthy roots, but the reduced ascorbate content was 3.5~7.5 times higher than that of the healthy roots. The total glutathione content of the epidermis, cortex and stele tissues in 겨sty roots was 7.3, 4.8, 1.2 times higher than the healthy tissues, respectively. The ratio of reduced glutathione (GSH) and oxidized glutathione (GSSG) showed a similar fluctuation of total glutathione content in 겨sty roots. These results indicate that the high antioxidative activity in rusty roots may involve in overcoming the oxidative stress derived from environmental stresses.

• Korean Journal of Medicinal Crop Science
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• v.8 no.1
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• pp.14-20
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• 2000

This experiment was conducted to investigate the effects of length of storage period under low temperature, $CO_2$ enrichment and addition of plant growth regulators in Murashige and Skoog medium on the plant regeneration of Korean ginseng (Panax ginseng C. A. Meyer). Seeds were treated for 60 and 80 days respectively under $5^{\circ}C$ environment. 2500ppm of $CO_2$ was enriched by ventilation. Three plant growth regulators added to the medium were Indolbutyric acid, Benzyladenin and Gibberellic acid (GA3). The result indicated that : The capacity of differentiation was higher in the aged cotyledons from the seeds treated for 80 days under low temperature condition than in those treated for 60 days. $CO_2$ enrichment had stimulating effects on the growth and development of shoot primordium significantly but less effects on the formation of adventitious buds. From one zygotic embryo hundreds of plantlets were differentiated. $CO_2$ enrichment had effects on the formation of both indirect somatic embryo and direct somatic embryo. Indirect somatic embryo showed little growth and differentiation, being undifferentiated vascular stele and epicotyl. Direct somatic embryos were formed on the epidermis of backside basal part of cotyledon. Those embryos developed to whole plant having latent bud.

• The Journal of the Korea Contents Association
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• v.11 no.1
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• pp.184-192
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• 2011

Cultural heritage is the historic symbol implying the life of people and regionality. Accordingly, the study on cultural heritage along the border between Korea and China is a very critical and urgent task for educational purposes as well as to secure historic and cultural awareness and national identity. The border area between Korea and China is classified into the areas along Aprok River and areas along Tumen River where the cultural heritage of various ethnicities is scattered. Accordingly, this study tried to find the approaches to implement and use the exhibition contents for educational purposes as well as visual applications rather than a literary study on cultural heritage in the border area between Korea and China. The results of this study will be the opportunity to enhance the practical understanding of the modern states as well as to learn the cultural awareness of the territory of a modern state and people's awareness of the importance of world heritages. Furthermore, the results of this study will be used as the resources for historic and cultural tours on the web or applications and help to understand the cultural features of Korea and China in the northeastern region as well as historic awareness in educational programs using exhibition materials in a museum.

• Journal of Korean Society of Forest Science
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• v.94 no.1 s.158
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• pp.16-20
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• 2005

Tricholoma matsutake ectomycorrhizas are unique in their morphology: not bifurcated broom shaped roots with not easily wettable brilliant and profuse white hyphae. To understand these characteristics the ectomycorrhizas were investigated with electron microscopy. T. matsutake ectomycorrhiza have thin mantle and typical Hartig net development in the epidermis and cortex, but no fungal mantle on the root apex. There were no penetrating hyphae inside of the cells of either epidermis, cortex or stele. Inside of the walls of epidermis and cortex cells are lined with ca. $2{\mu}m$ hemispherical amyloplasts. The brilliant hyphal surface was covered with various fine amorphous granules. The hyphal cell wall was thin membrane less than $0.3{\mu}m$ thick. There is no clamp connection on the hyphae. This thin membraneous cell wall with high elasticity can be related to survival strategy of the species without plasmolysis under frequent soil water stress environment. And the coarse hyphal surface with some water repellency can control sudden inrush of water of the hyphae with an extremely low osmotic potential. It is concluded that no mantle on the tip can induce mycorrhizas not bifurcated and that finely coarse surface of T. matsutake hyphae can make the hyphae brilliantly white but less wetted.

• Journal of Ginseng Research
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• v.38 no.4
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• pp.270-277
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• 2014

Background: The effect of methyl jasmonate (MJ) on ginsenoside production in different organs of ginseng (Panax ginseng Meyer) was evaluated after the whole plant was dipped in an MJ-containing solution. MJ can induce the production of antioxidant defense genes and secondary metabolites in plants. In ginseng, MJ treatment in adventitious root resulted in the increase of dammarenediol synthase expression but a decrease of cycloartenol synthase expression, thereby enhancing ginsenoside biosynthesis. Although a previous study focused on the application of MJ to affect ginsenoside production in adventitious roots, we conducted our research on entire plants by evaluating the effect of exogenous MJ on ginsenoside production with the aim of obtaining new approaches to study ginsenoside biosynthesis response to MJ in vivo. Methods: Different parts of MJ-treated ginseng plants were analyzed for ginsenoside contents (fine root, root body, epidermis, rhizome, stem, and leaf) by high-performance liquid chromatography. Results: The total ginsenoside content of the ginseng root significantly increased after 2 d of MJ treatment compared with the control not subjected to MJ. Our results revealed that MJ treatment enhances ginsenoside production not in the epidermis but in the stele of the ginseng root, implying transportation of ginsenosides from the root vasculature to the epidermis. Application of MJ enhanced protopanaxadiol (PPD)-type ginsenosides, whereas chilling treatment induced protopanaxatriol (PPT)-type ginsenosides. Conclusion: These findings indicate that the production of PPD-type and PPT-type ginsenosides is differently affected by abiotic and biotic stresses in the ginseng plant, and they might play different defense mechanism roles.

• Journal of Conservation Science
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• v.7 no.1
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• pp.9-18
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• 1998

There are about 1470 stone-cultural properties in Korea which have been registered and protected by the government. Representative of them are pagoda, stupa, stone-buddha, stele support of banner pole and stone-lanterns. Most of them have been exposed to and weathered by natural environments. Stone-cultural properties are destroyed and/or destructed mostly by weathering and its related features of rocks. Therefore, it is necessary to conduct geological studies on the weathering phenomena and related features as well as characteristics of the rock itself must be done for the conservation. Thus geological investigation should include detail measurements, rock varieties and phases, structural lineaments of the rock, cause and degree of weathering, degree and patterns of crack development structural stability, identification of reusable parts for restoration, geomorphological characteristics of the site, and etc. The interaction among these factors must be investigated and analyzed, which must be used as basic data to establish the guideline of conservation and to plan for repair and/or restoration. The conservation plan should involve parts to be repaired, method of repair, type of cementing material, the characteristics of rock phases to be supplemented, method for maintaining structural stability, and method to delay or to prevent the rock weathering.

• Journal of Korean Society of Forest Science
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• v.89 no.3
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• pp.389-396
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• 2000

To determine whether the Tricholoma matsutake (pine mushroom, Songyi) is symbiotic or parasitic to Pinus densiflora, structural change in their natural ectomycorrhizas were examined. The mycorrhizal samples were collected at three progressional points in the natural hypogeous colony(shiro) : colony front edge, near the fruiting point and 20cm back. The fine roots in the colonies were typical ectomycorrhizas with fungal mantle and Hartig net. However, the T. matsutake mycorrhizas had unique characteristics compared to other types of ectomycorrhizas. That is, spatially the fungal mantle and Hartig net of the T. matsutake mycorrhizas continued to develop along the growing tip, while temporally those structures declined to shrink changing to black brown in the older part of the roots behind the actively growing tip portion. However, there was no mark that the fungal hyphae penetrated into either the cortical cells, endodermal cell layers or stele. The apical tips of the blackened roots remained alive to form new mycorrhizas with other fungi later. Therefore, we conclude that the mycorrhiza of T. matsutake+P. densiflora is rather a dynamic symbiosis that changes its position spatiotemporally as the root grows than either a simple parasitism or symbiosis.