• Food Science and Preservation
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• v.12 no.3
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• pp.263-266
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• 2005

Five strains out of tweeny four strains of lactic acid bacteria isolated from Mul-Kimchi showed a resistance in the artificial gastric juice. These lactic acid bacteria were identified as Lactobacillus plantarum species. In particular, lactobacilli MUL-2, MUL-4 and MUL-18 showed a strong resistance and their viable cell counts of the initial stage were not changed after the 3 h cultivation in the artificial gastric juice. The isolated lactic acid bacteria showed the antimicrobial effect against Listeria monocytogenes, Escherichia coli, Staphylococcus aureus, Vibrio vulnificus, and Salmonella typhimurium in the paper disc method.

• Journal of environmental and Sanitary engineering
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• v.5 no.2
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• pp.53-62
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• 1990

There has been considerable organisms the most indicative of the sanitary quality of food products. Of the suggested indices of sanitary quality of foods are coliform organism and SPC. In addition to the usual index organisms on fish products it is also necessary to determining the sanitary quality The authors have tested with 282 fish products (spring : 39, summer : 109, autumn : 112, winter . 22) 1. The range of microbial organism in fish products are as follows Spring : SPC is $13{\times}10^3\; to\; 50{\times}10^8/g$, coliform group is $16{\times}10^2\; to\; 48{\times}10^8/g$ and 2. coli is 50 to $22{\times}10^4/100g$. Summer : SPC is 70 to$64{\times}10^9/g$. coliform group is 25 to $26{\times}10^8/g$ and E. coli is 20 to $22{\times}10^4/100g$. Autumn : SPC is $10{\times}10^3\; to\; 46{\times}10^8/g$, coliform group is 200 to $20{\times}10^5/g$ and E. coli is 20 to $22{\times}10^4/100g$. Winter : SPC is $30{\times}10^3\; to\;30{\times}10^8/g$. coliform group is $21{\times}10^2\;to\;16{\times}10^3/g$ and E. coli is 20 to 790/100g. Salmonella and Staphylococcus species were not in 282 fish products.

• Journal of Microbiology and Biotechnology
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• v.8 no.2
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• pp.111-118
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• 1998

A bacterium producing unique antistaphylococcal substance (ASS) was isolated from soil samples. The isolated strain KUH-001 was identified to belong to Pseudomonas species from the characteristic properties of its fluorescence and cellular 3-hydroxy fatty acid composition, etc. The ASS component was purified by procedures employing activated carbon adsorption, column chromatography with silica gel, preparative TLC and HPLC. This compound could also be purified mainly by repeating of trituration and precipitation with chilled ether. Purified ASS with a m.p. value of $140~142^{\circ}C$ showed marked stability at high temperature (at $121^{\circ}C$ for 10 min) and extreme pHs (in 1N HC1 and 1N NaOH for 1 day) without significant loss of antibiotic activity. From spectral data of UV, IR, NMR, and FAB-MS, the compound was elucidated as 2-heptyl-4-hydroxyquinoline N-oxide (HHQO). Under the conditions employed, HHQO exhibited a narrow antimicrobial spectrum. active particularly against Staphylococcus aureus including the methicillin resistant strain. Moreover, it did not induce resistance, and besides, interacted synergistically with certain antibiotics such as vancomycin or erythromycin.

• YAKHAK HOEJI
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• v.38 no.6
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• pp.742-748
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• 1994

As part of our search for less toxic antimicrobial agents from natural resources, the carpophores of Elfvingia applanata$(P_{ers}.)K_{ARST}.$ was extracted with hot water. EA, the aqueous extract from the carpophores of E. applanata, was lyophilized and a dark brownish powder was obtained. Antimicrobial activity of EA was tested in vitro against Gram positive and Gram negative bacteria by serial broth dilution method, and the antimicrobial activity was expressed by minimal inhibitory concentration(MIC). Among fourteen species of bacteria tested, the antimicrobial activity of EA was the most potent against Proteus vulgaris showing MIC of 1.250 mg/ml. To investigate the effect of antimicrobial combinations of EA with four kinds of antibiotics(ampicillin, cefazolin, oxytetracycline and chloramphenicol), the fractional inhibitory concentration index(FICI) was determined by checkerboard assay for each strain. The antimicrobial combinations of EA with four kinds of antibiotics resulted in synergism in four instances, but no antagonism was observed. Four instances of synergism were observed when EA was combined with ampicillin against Micrococcus luteus, with cefazolin against Bacillus subtilis, with cefazolin against Micrococcus luteus and with oxytetracycline against Staphylococcus aureus.

• Journal of Korean Academy of Nursing
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• v.14 no.2
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• pp.55-62
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• 1984

This study was performed from september 26th to October 7th 1981 to investigate the contamination problems of Nurse's hands characterized by var-ious nursing functions. A total of 50 nurse's hands were sampled from 5 different wards of H. University Hospital. The samples were cultured for isolation of microorganisms. The results were as follows: 1. Of 50 Nurses 23 were found to be contaminated by 9 species of bacilli such as Non-fermentative gram negative Bacilli, Gram negative bacilli, Oxidase positive, Enterobacter, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter, Staphylococcus epidermidis, Gaffkya tetragens, Bacillus subtilis. 2. The contaminate rates by wards where they have been serving are; 7 (87.5％) of 8 nurses from Intensive care unit, 7(70%) of 10 nurses from general surgery ward, 3(50%) of 6 nurses from neurosurgery ward, 2(20%) of 10 nurses from orthopedic surgery ward, 4(25%) of 10 nurses from medical ward. 3. The contamination rates by the types of clinical service offered are 6(85.7%) of 7 nurses after wound dressing assist 6 (55.3%) of 13 nurses after vital sign check. 4. No statistical significance could he observed as to the between the rates of contamination of nurse's hands with various nursing functions (0.1

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.3
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• pp.415-420
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• 2001

Eighteen kinds of medicinal edible herbs, which are nontoxic and has been widely used in traditional folk medicine, were extracted and antimicrobial activity of the extracts was investigated against various foodborne pathogens or food poisoning microorganisms. Among them, the ethanol extract of Quercus mongolica showed the strongest antimicrobial activities against Gram positive and Gram negative bacteria and followed by Quercus aliena and Quercus dentata, respectively. Thus, further study was conducted to determine the antimicrobial activity of Quercus species extracts. The plants were extracted with ethanol, methanol and water, respectively. The ethanol, methanol, and water extracts of Quercus mongolica leaf showed 10~21 mm inhibition zone against Gram positive and Gram negative bacteria at two thousand $\mu\textrm{g}$ per disc, but little antimicrobial activity was observed against fungi and yeast. The minimal inhibitory concentrations (MIC) of the ethanol extract of Quercus mongolica leaf was 250$\mu\textrm{g}$/mL against Bacillus cereus. Salmonella typhimurium, and Pseudomonas aeruginosa and 62.5~125 $\mu\textrm{g}$/mL against Staphylococcus aureus and Listeria monocytogenes, respectively.

• Biomedical Science Letters
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• v.19 no.3
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• pp.245-253
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• 2013

To provide reference data or guideline for empirical treatment of bloodstream infection, we studied a change pattern in causative microorganisms and antimicrobial susceptibility in a general hospital at Gyeonggi province during five years. We retrospectively reviewed the frequency of causative microorganisms and antimicrobial susceptibility results of 5,782 microorganisms isolated from blood culture in a general hospital during the period from January 2008 to December 2012. The most common pathogens were Escherichia coli (14.7%), Staphylococcus aureus (7.5%), Streptococcus viridans group (4.9%), and Klebsiella pneumoniae (4.1%). The multiple microorganisms were isolated in 4.3% of bloodstream infection patients. The average contamination rate of blood culture during five years was 3.0%. Methicillin-resistant S. aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), and penicillin-resistant Streptococcus pneumoniae were isolated at 62%, 27% and 11%, respectively. Cefotaxime-resistant E. coli and K. pneumoniae was 20% and 18%, respectively. Imipenem-resistant Pseudomonas aeruginosa (IRPA) and Acinetobacter baumannii (IRAB) was 25% and 66%, respectively. E. coli and S. aureus were most common pathogens isolated from blood culture for five years. The increase of multidrug-resistant microorganisms, such as MRSA, VRE, ESBL, IRPA and IRAB, requires more strict control of antibiotics and causes the need of the more updated guideline for the treatment of blood stream infection.

• Korean Journal of Veterinary Service
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• v.17 no.3
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• pp.174-180
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• 1994

The disease syndrome characterized by the acute vomiting and diarrhea with high mortality had been greatly epidemic in Kyungbuk West Area since March 1990 and it was followed serologically for the classification of the agent. The agent present in feces of dogs associated with this syndrome had characteristic feature in agglutinating pig red blood cells that was specifically inhibited by anti-CPV reference dog serum. This also showed the serological identity with the reference CPV antigen in Hemagglutinating inhibition test. The result obtained were summarized as follows : 1. During 5 years(March. 1990∼September. 1994), 1,470 dogs were investigated on the actual condition of CPV infections. The Infection rate of CPV from dogs was 62.5% and mortality rate was 59.8%. 2. Among 24 fecal samples collected from the dogs with enteric disease, all showed the hemagglutinating activity to porcine erythrocyte ranging from 40 to 5,120 of HA titers. 3. Among 12 sera samples collected from the dogs with enteric disease, all showed the serological identity with the reference CPV antigen from 5 to 5,120 of HI titers. 4. Bacteriologic examination of fecal specimens resulted in the isolation of pathogeric bacteria such as Staphylococcus sp, Streptococcus sp, Escherichia coli and Bacillus. Cultures for salmonella sp and Clostridium remaind negative. 5. The prevalence and identification of internal parasites were determined by fecal examination using the floatation methods. From 20 fecal samples 12(60.0%) were isolated and their species were Toxacara canis, Toxascaris leonina, and coccidium.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.5
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• pp.495-504
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• 1997

Three media, i. e., MOD-SD, M98-5 and M98-5 supplemented with chicken fecal extract were tested as isolation media for anaerobic bacteria present in the duodenum, jeju-ileum and cecum of chicken. The results showed that the mean colony counts of medium M98-5 were similar with those of MOD-SD medium in all intestinal samples at the incubation periods of 2, 6 and 10 days. Supplementation with chicken fecal extract of M98-5 medium significantly increased (p < 0.05) the colony counts of bacteria from the duodenum, jeju-ileum and cecum. The colony counts at 6-day incubation were similar with those at 10-day incubation, but were much higher than the counts at 2-day incubation. The major types of bacteria found in the duodenum and jeju-ileum of chicken were tentatively identified as Lactobacillus, Streptococcus and E. coli. In the cecum, ten tentatively identified groups of bacteria, namely, Streptococcus, Staphylococcus, Lactobacillus, E. coli, anaerobic coccus, Eubacterium, Propionibacterium, Clostridium, Fusobacterium and Bacteroides were isolated. Anaerobes were found to comprise nearly the entire microbial population of the cecum. Predominating in all sections of the intestine were homofermentative lactobacilli. The main Lactotacillus species in chicken intestine were L. acidophilus, L. fermentum and L. brevis.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.19 no.1
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• pp.87-92
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• 1997

Treatment of infected mandibular fracture is confronted with various difficult problem, e.g. nosocomial wound infection, non-union of fracture, osteomyelitis. Recently, nosocomial infection has become a major health problem because of excessive morbidity, personal distress, and cost. Frequently, isolated causative microorganisms of nosocomial infection were staphylococcus aureus, pseudomonas aeruginosa, klebsiella species. The various manifestation of the disease related to the pathogenesis and the clinical course tend to give a bad prognosis after operation. This is a report of case that post-operative infected mandibular fracture in 53-year-old man was not healed even through aggressive I & D and antibiotic treatment.