• The Journal of the Korea Contents Association
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• v.13 no.2
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• pp.175-187
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• 2013

Because of the governments policy to promise the cultural homesickness of citizens, national and public culture centers are under construction all over the country. The interest of watching plays are increasing. But the existing culture center's facilities operating in the construction of emphasized. It's made up of public officials in technical service, are having a hard time breaking their limits. In consequence, to solve these problems it will be done with people who have qualifications of stage art professional in the center. Productions raise a support system of the city and venues subject to theatre arts professionals as a way of solving these problems. These are the efficient ways to develop performance cultures of issues presented and the Busan area performing arts Activation Plan is proposed.

• Korean Journal of Veterinary Research
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• v.41 no.1
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• pp.1-6
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• 2001

Pituitary adenyl ate cyclase activating polypeptide (PACAP) was originally isolated from the ovine hypothalamus and stimulated cAMP production in anterior pituitary cells. It is known that PACAP stimulates cAMP accumulation and contributes to the spermatogenesis and steroidogenesis in rat testis. The principal aim of this study is to determinate the distribution of PACAP mRNA and protein in adult rat testis. For this study, we used in situ hybridization and immunohistochemistry techniques in adult rat testis. PACAP mRNA was stage specifically expressed in seminiferous tubules. Positive signals of PACAP mRNA were detected in the developing germ cells at stages HI-VII of the epithelial cycle. The strongest signals of PACAP mRNA and protein were detected in round spermatids at stages V to early VII of the cycle. These results demonstrate that PACAP which is synthesised in the developing germ cells contributes to the spermatogenesis in rat testis. Thus, we suggest that PACAP plays a critical role in the function of testis.

• Applied Microscopy
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• v.32 no.1
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• pp.31-37
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• 2002

As-cast microstructure of Mg-rich $Mg_{68}Zn_{28}Y_4$ has been investigated by a detailed transmission electron microscopy. The as-cast $Mg_{68}Zn_{28}Y_4$ alloy consisted of three different types of phases: $10{\sim}20{\mu}m$ size primary solidification phase, dendritic phase grown from the primary phase and a eutectic structure formed at the later stage of solidification. The primary solidification phase has an icosahedral structure with a large degree of phason strain. 1/1 rhombohedral approximant phase with lattice parameters: $a=27.2{\AA}\;and\;{\alpha}=63.43^{\circ}$ is first observed in Mg-Zn-Y system. The rhombohedral structure can be obtained by introducing phason strain in the six dimensional face centered hyper-cubic lattice. The decagonal phase nucleates with orientation relationship with the icosahedral phase, and $Mg_4Zn_7$ nucleates with orientation relationship with the decagonal phase, indicating a close structural similarity between the three phases. Gradual depletion of Y during solidification plays an important role in heterogeneous nucleation of decagonal and $Mg_4Zn_7$ phases from icosahedral and decagonal phases respectively.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.6
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• pp.71-85
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• 2011

In Okdong coal mine abandoned area, every year large amount of mine wastes have been swept away due to heavy rains in summer. Because pH of coal-mine waste is in the early 4, plant naturally does not grow there due to unfavorable condition for growing plant. This study had an experiment to grow plant for 6 months using Mycorrhizae which can be adapted well in acid soil. As a result of Experiment, In the infected experiment pot, 90% of the plants survived but in case of uninfected experiment pot, only 25% were alive. From Growth Experiment, it appeared that average stem length increase (cm/month), average fresh weight increase ($g^{fw}/month$), average dry weight increase ($g^{dw}/month$) were increased by 60%, 21%, 31% respectively. Especially, Mycorrhizae plays an important role in providing nutrients and water when seeding are established. Mycorrhizae prevents death caused by lack of nutrients and water and helps growth and development of plant when seeding are established in the early stage. This study proved that Mycorrhizae is comparatively effective in plant growth and prevention of erosion in coal-mine abandoned area.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2008.04a
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• pp.235-240
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• 2008

The environment has played a key role to improve the living condition and develop the industry. In building industries, we should consider the environment and mitigate the environmental affect. For mitigating the its affect, various areas of building technology have been developed and applied into filed work. In addition, the process in applying into field requires to conduct the assessment of the environmental affect and improve its applied technology. A lot of assessment methods are proposed in evaluate the building condition such as post-occupancy evaluation, life cycle management and life cycle assessment. Among these assessment methods, life cycle assessment is effectively utilized the environmental affect in building life cycle. Therefore, this paper aimed at analyzing the energy consumption and $CO_2$ emission in building life cycle, using the life cycle assessment and application of the example in apartment housing. This study shows that the maintenance and the production of building materials stage shares most of the amount of energy consumption and $CO_2$ emission and therefore plays an important role to planning the building in terms of the life cycle. Second, the other stages brings about a very small amount. It is important to decide the building shape and contents to mitigate the environmental affect in terms of material, volume, the pattern of the energy use and others.

• Korean Journal of Construction Engineering and Management
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• v.10 no.3
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• pp.139-147
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• 2009

Accurate construction cost estimation in the initial stage of building project plays a key role for project success and for mitigation of disputes. Total construction cost(TCC) estimation of apartment projects in Vietnam has become more important because those projects increasingly rise in quantity with the urbanization and population growth. This paper presents the application of artificial neural networks(ANNs) in estimating TCC of apartment projects. Ninety-one questionnaires were collected to identify input variables. Fourteen data sets of completed apartment projects were obtained and processed for training and generalizing the neural network(NN). MATLAB software was used to train the NN. A program was constructed using Visual C++ in order to apply the neural network to realistic projects. The results suggest that this model is reasonable in predicting TCCs for apartment projects and reinforce the reliability of using neural networks to cost models. Although the proposed model is not validated in a rigorous way, the ANN-based model may be useful for both practitioners and researchers. It facilitates systematic predictions in early phases of construction projects. Practitioners are more proactive in estimating construction costs and making consistent decisions in initial phases of apartment projects. Researchers should benefit from exploring insights into its implementation in the real world. The findings are useful not only to researchers and practitioners in the Vietnam Construction Industry(VCI) but also to participants in other developing countries in South East Asia. Since Korea has emerged as the first largest foreign investor in Vietnam, the results of this study may be also useful to participants in Korea.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.38 no.2
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• pp.101-109
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• 2012

Objectives: The inactivation of the tumor suppressor gene $p16^{INK4a}$ plays an important role in the development of malignant tumors, including oral squamous cell carcinoma. The p16 gene is involved in the p16/cyclin-dependent kinase/retinoblastoma (Rb) gene pathway of cell cycle control. The p16 protein is considered a negative regulator of this pathway. The p16 gene encodes an inhibitor of cyclin-dependent kinases 4 and 6 which regulate the phosphorylation of the retinoblastoma gene and G1 to S phase transition in the cell cycle. However, the p16 gene can lose its functionality through point mutations, loss of heterozygosity or methylation of its promoter region. Materials and Methods: In this study, the authors analyzed the correlation between various clinicopathological findings- patient age, gender and smoking, disease recurrence, tumor size, stage, and differentiation- and p16 protein expression or p16 promoter hypermethylation in 59 cases of head and neck squamous cell carcinoma. Results: The results revealed p16 protein expression and p16 promoter hypermethylation in 28 cases (47.5%) and 21 cases (35.6%), respectively, of head and neck squamous cell carcinoma. However, neither p16 protein expression nor p16 promoter hypermethylation had any statistical influence on clinicopathological findings or survival rate. Conclusion: This data, and a review of the literature, suggest that p16 promoter hypermethylation cannot yet be used as an independent prognostic factor influencing carcinogenesis, but must be considered as an important factor along with other genetic alterations affecting the pRb pathway.

• Journal of Digital Contents Society
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• v.10 no.3
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• pp.461-468
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• 2009

In a recent year, the broadcasting system has been changed from analogue to digital all over the world, not just in Korea, and Korea aims at accomplishing 100% digital broadcasting by 2013. The HD(High Definition) Video Format, which currently plays a key role in digital broadcasting system, has been gradually extended in the field of broadcasting and movie. Additionally, many kinds of camcoders and editing systems have been rapidly converted into the digital types, and they are supporting to photograph and video editing systems with the various types of image formats such as SD(Standard Definition) format, HD format, 2K, 4K, etc. Tape Video Storage System, which has been usually used in the stage of photographing by this day, is gradually evolving into Tapeless Video Data Storage System. Thus in this study, Video Compression method of HD format photographed by Sony PMW-EX3, one of major tapeless camcoders, is studied and also the way of applying it effectively in Avid Media Composer, a non-linear editing system, is discussed.

• Animal cells and systems
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• v.13 no.4
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• pp.439-445
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• 2009

Cytochrome P450 aromatase (P450arom) catalyzes the conversion of androgens to estrogens and plays an important role in reproduction and development in vertebrates. We investigated the expression patterns of ovarian P450arom (P450aromA) and brain P450arom (P450aromB) mRNA during sex change in black porgy. Maturity was divided into seven stages from male to female (immature testis, mature testis, testicular portion of mostly testis, ovarian portion of mostly testis, testicular portion of mostly ovary, ovarian portion of mostly ovary, and mature ovary). P450aromA expression was significantly higher in the ovarian portion of mostly-ovarian stage fish, and P450aromB expression was highest in the brain of black porgy with mostly-ovarian gonads. Histology showed that testicular tissues were disintegrated with the development of ovarian tissue associated with an increase in the expression of the two P450arom mRNAs during sex change. Interestingly, among various tissues, P450aromA was only expressed in the ovary, and P450aromB was only expressed in the brain. To understand the role of gonadotropin-releasing hormone (GnRH) and estradiol ($E_2$), we injected exogenous hormone (GnRH analogue [GnRHa] and $E_2$) into immature black porgy. In the GnRHa group, expression of the two P450arom genes decreased 12 h after injection, and expression of the two P450arom genes were significantly higher at 6 dafter $E_2$ injection. These results provide useful baseline knowledge on the mechanism of natural sex change in black porgy.

• Proceedings of the Botanical Society of Korea Conference
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• 1996.07a
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• pp.48-60
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• 1996

We previously identified and characterized a predominantly pollen-expressed gene of Petunia inflata that encodes a receptor-like kinase named PRK1. The extracellular domain of PRK1 contains leucine-rich repeats which have been implicated in protein-protein interactions, and the cytoplasmic domain was found to autophosphorylate on serine and tyrosine. To investigate the function PRK1 in pollen development, we transformed P. inflata plants with a construct containing the promoter of a predominantly pollen-expressed gene of tomato, LAT52, fused to an antisense PRK1 cDNA corresponding to part of the extracellular domain of PRK1, There transgenic plants were found to each produce approximately equal amounts of normal and aborted pollen. Analysis of the inheritance of the transgene inserts in two of the transgenic plants, ASRK-13 and ASRK-20, to their progeny revealed that certain transgene inserts cosegregated with the pollen abortion phenotype. Microscopic examination of the aborted pollen grains showed that their outer wall, the exine, was essentially normal, but that their cytoplasm contained only starch-like granules. Staining of the nuclei of the microspores at different stages of uninucleate stage. However, at subsequent stages half of the microspores completed mitosis and developed into normal binucleate pollen, but the other half initially remained uninucleate, then lost their nucleio. Analysis of the amounts of PRK1 mRNA and the antisense PRK1 transcript suggested that the pollen abortion phenotype most likely resulted from down-regulation of the PRK1 gene by the antisense PRK1 transgene. These results suggest that PRK1 plays an essential role in a signal transduction pathway that mediates post-meiotic development of microspores.