• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7603-7609
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• 2014

Glutathione is a thiol compound that plays an important role in the antioxidant defense system of the cell and its deficiency leads to an increased susceptibility to oxidative stress and, thus, progression of many disease states including head and neck cancer. In the present study, alterations of glutathione levels were investigated in study cohort of 500 samples (cohort 1 containing 200 head and neck cancer blood samples along with 200 healthy controls and cohort II with 50 head and neck squamous cell carcinoma tissue samples along with 50 control tissues) by high performance liquid chromatography. The results indicated that mean blood glutathione levels were significantly reduced in head and neck cancer patients (p<0.001) compared to respective controls. In contrast, the levels of glutathione total (p<0.05) and glutathione reduced (p<0.05) were significantly elevated in head and neck squamous cell carcinoma tissues compared to the adjacent cancer-free control tissues. In addition to this, pearson correlation performed to correlate different tissue glutathione levels (GSH) with clinical/pathological parameters demonstrated a significant negative correlation between pT-stage and GSH level ($r=-0.263^{**}$; p<0.01), C-stage and GSH level ($r=-0.335^{**}$; p<0.01), grade and GSH ($r=-0.329^{**}$; p<0.01) and grade versus redox index ($r=-0.213^{**}$; p<0.01) in HNSCC tissues. Our study suggests that dysregulation of glutathione levels in head and neck cancer has the potential to predict metastasis, and may serve as a prognostic marker.

• 한국발생생물학회지:발생과생식
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• 제21권2호
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• pp.131-138
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• 2017

This study was conducted to investigate stimulatory effect of epidermal growth factor (EGF) on nuclear maturation and the expression level of EGF-receptor (EGFR), GM-130 (a marker of Golgi apparatus), transport protein Sec61 subunit beta ($Sec61{\beta}$), and coatomer protein complex subunit gamma 2 (COPG2) in porcine oocytes. The cumulus-oocyte complexes were collected from follicle with 3-6 mm in diameter. They were incubated in medium with/without EGF for 22 h (IVM I) and subsequently incubated hormone-free medium with/without EGF for 22 h (IVM II). Nuclear maturation state was checked by aceto-orcein stain. Protein expression of EGFR, GM-130, $Sec61{\beta}$, and COPG2 were measured by immunofluorescence. In results, nuclear maturation of oocytes in EGF non-treated oocytes were significantly lower than EGF-treated groups at IVM I or IVM II stage (P<0.05), whereas maturational rate in EGF treatment groups at both of IVM stage was higher in among the all treatment groups (P<0.05). EGFR, GM-130, $Sec61{\beta}$ and COPG2 were expressed in the cytoplasm of oocytes. Especially, GM-130 and EGFR were strongly expressed, but $Sec61{\beta}$ and COPG2 were weakly expressed in cortical area of cytoplasm. The protein level of GM-130, $Sec61{\beta}$, and COPG2 were significantly higher in the EGF-treated groups (P<0.05). However EGFR was no difference between non EGF-treated groups and control. In conclusion, EGF plays an important role in the systems for oocyte maturation with endoplasmic reticulum and Golgi apparatus. In addition, the protein levels of $Sec61{\beta}$ and COPG2 could be changed by EGF in the porcine oocytes during maturation.

• Asian Pacific Journal of Cancer Prevention
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• 제15권8호
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• pp.3741-3745
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• 2014

Background: Emerging evidence implicates the platelet-derived growth factor-D (PDGF-D) in many types of human solid tumors. We investigated whether PDGF-D plays an important role in endometrial cancer (EC) in relation to clinicopathologic phenotype, angiogenesis, and patient prognosis. Materials and Methods: We analyzed PDGF-D protein expression by Western blotting in twenty-seven human endometrial cancer tissues, and matched normal endometrial controls collected at the third Affiliated hospital of Sun Yat-sen University during 2012-2013 (n=27). Immunohistochemical staining was performed using a human PDGF-D antibody on the endometrial cancer patients collected in the same facility during January 2001 and October 2013 (n=152). Patients were followed from the time of primary surgery in 2001-2013 until death or last follow-up. We correlated the PDGF-D expression levels with clinicopathologic parameters and prognosis in human endometrial cancer patients. Results: Compared with matched normal endometrial cases, PDGF-D was up-regulated in endometrial cancer. Expression of PDGF-D protein, found in 78% of the cases, was associated with nonendometrioid histologic type (p=0.028), FIGO stage III/IV (p=0.039), >50% solid tumor growth (p=0.048), pelvic LN metastasis (p=0.035) and ER and PR negativity (p=0.04 and 0.002). PDGF-D expression was also significantly associated with expression of VEGF-A (p=0.021). In multivariate analysis, PDGF-D expression proved to be an independent prognostic factor in addition to histologic grade and FIGO stage. Patients with high expression levels of PDGF-D had a significantly poorer overall survival rate compared with patients with no expression. Conclusions: PDGF-D expression is frequently up-regulated in endometrial cancer, and is associated with aggressive features and poor prognosis.

• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.2851-2857
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• 2013

Objective: REPS2 plays important roles in inhibiting cell proliferation, migration and in inducing apoptosis of cancer cells, now being identified as a useful biomarker for favorable prognosis in prostate and breast cancers. The purpose of this study was to assess REPS2 expression and to explore its role in esophageal squamous cell carcinoma (ESCC). Methods: Protein expression of REPS2 in ESCCs and adjacent non-cancerous tissues from 120 patients was analyzed by immunohistochemistry and correlated with clinicopathological parameters and patient outcome. Additionally, thirty paired ESCC tissues and four ESCC cell lines and one normal human esophageal epithelial cell line were evaluated for REPS2 mRNA and protein expression levels by quantitative RT-PCR and Western blotting. Results: REPS2 mRNA and protein expression levels were down-regulated in ESCC tissues and cell lines. Low protein levels were significantly associated with primary tumour, TNM stage, lymph node metastasis and recurrence (all, P < 0.05). Survival analysis demonstrated that decreased REPS2 expression was significantly associated with shorter overall survival and disease-free survival (both, P < 0.001), especially in early stage ESCC patients. When REPS2 expression and lymph node metastasis status were combined, patients with low REPS2 expression/lymph node (+) had both poorer overall and disease-free survival than others (both, P < 0.001). Cox multivariate regression analysis further revealed REPS2 to be an independent prognostic factor for ESCC patients. Conclusions: Our findings demonstrate that downregulation of REPS2 may contribute to malignant progression of ESCC and represent a novel prognostic marker and a potential therapeutic target for ESCC patients.

• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.5055-5060
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• 2013

Objective: Twist, a basic helix-loop-helix transcription factor, plays a key role in the metastatic progression of human cancer. Matrix metalloproteinase (MMP)-9 is an endopeptidase that digests basement membrane type IV collagen, therefore being possibly related to tumor progression. It has been reported that Twist and matrix metalloproteinase-9 (MMP-9) are expressed in gastric cancers. However, the exact roles of Twist and MMP-9 in tumor metastasis and prognosis remain unclear. The aim of this study was to casts light on this question. Methods:Twist and MMP-9 expression in tissue sections of 37 gastric carcinomas was evaluated with immunohistochemistry. The staining results were compared with clinicopatholgic features and to patients' outcome. Results: Twist positive expression was significantly increased in gastric cancer cases with lymph node metastasis (P=0.023). But no correlations were found between MMP-9 overexpression and clinicopathologic features, such as recurrence, TNM stage, and lymph node metastasis. Overall survival (OS) was significantly correlated with recurrence, serosa invasion, TNM stages, distant metastasis, and MMP-9 (P=0.027, 0.021, 0.000, 0.024 and 0.036, respectively). Disease-free survival (DFS) was prominently related to recurrence location, serosa invasion and TNM stages (P=0.000, 0.038 and 0.003, respectively). In the Cox regression multivariate analysis, TNM stage, distant metastasis and MMP-9 were significantly associated with prognosis of gastric cancer (P=0.002, 0.019, and 0.032, respectively). Conclusions: This study showed Twist positive expression to be significantly correlated with lymph node metastasis in gastric cancer. MMP-9 overexpression is associated with OS, suggesting that MMP-9 is a prognostic indicator for survival in patients with gastric cancer.

• 패션비즈니스
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• 제5권2호
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• pp.149-166
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• 2001

The purpose of this study was illumination of the stage costume by applying the study of a subject of human expression, which establishes identity of dramatic characters. This study was conducted by referring a variety of theses, an extensive national and international literature. The color symbolism that is depicted in Shakespeare‘s work manifests the transition period of old days, which was influenced by the Renaissance and the Religious Reformation. And color preferences and the meanings I attach to them in his play, definitely reveal the Middle Ages Christian way of thinking which was obsessively dominated their mind. I also determine that the color preferences and use in Elizabeth Era also distinctively separate the social status, which were also influenced by its social conditions. Besides this, colors that were depicted in Shakespeare's work also shown many similarities from Italian Commedia dell‘arte, which were in vogue all over the Europe. Customary color symbolisms which from natural color experiences were also applied in "The Tempest". "The Tempest" was presented on the first day of November in 1611 for the first time. Following this, many plays and films have been produced in foreign countries and Korea. With an analysis of "The Tempest", costume designs for Miranda and Ferdinanad were illustrated at the end. The costumes for Miranda and Ferdinand were mainly considered to express pure love and vigorous youth of two lovers, symbolizing the image of the theme love, forgiveness, and reconciliation.

• Imaging Science in Dentistry
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• 제50권3호
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• pp.209-216
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• 2020

Purpose: In India, the age of 14 years is the legal age threshold for child labour. Therefore, in suspected instances of child labour, age assessment plays a crucial role in determining whether a violation of the law on the employment of children has occurred. The aim of this retrospective cross-sectional study was to assess the discriminatory ability of stages of cervical vertebral maturation (CVM) in predicting the legal age threshold of 14 years. Materials and Methods: Routinely taken lateral cephalograms from 408 subjects aged 10 to 18 years were evaluated retrospectively using the CVM stages described by Baccetti et al. Descriptive statistics, accuracy, sensitivity, specificity, positive and negative predictive values, and likelihood ratios were calculated for stages 2, 3, and 4 of CVM. Results: Real age increased as the CVM stage gradually increased. The results of 2×2 contingency tables showed that CVM stage 4 produced an accuracy of 71% and 73%, a false positive rate of 7% and 18%, and a post-test probability of 59% and 68% for boys and girls, respectively. Conclusion: Based on these findings, it can be concluded that the stages of CVM are of limited use for predicting the attainment of the legal age threshold of 14 years. Future studies should investigate whether combinations of skeletal and dental methods could achieve better accuracy and post-test probability.

• 한국음향학회지
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• 제19권5호
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• pp.13-19
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• 2000

음성신호의 음소경계 추출방법 중 음소에 대한 사전지식 없이 음성 데이타, 혹은 특징벡터의 변화를 감지하여 음소경계를 추출해 내는 맹목 세그먼테이션은 연속음형 인식시스템이나 코퍼스 제작에 중요한 역할을 하며 많은 연구가 진행되어 왔다. 이러한 맹목 세그먼테이션 방법은 사전지식을 필요로 하지 않아 비교적 쉽게 접근할 수 있으나 음운학적인 지식, 또는 음소나 음소경계에 대한 지식과 경험 데이타 등을 이용하는 지식 기반 세그먼테이션 방법에 비해 성능이 좋지 못한 단점이 있다. 본고에서는 우리말의 연속 음성을 맹목 세그먼테이션해서 후보 경계를 추출한 다음, 음절핵의 위치정보를 이용하여 후보 경계를 후처리함으로써 세그먼테이션 효율을 높이는 방법을 제안한다. 제안하는 방법의 전처리과정에서는 확률적인 거리 모델을 이용한 클러스터링 방법을 이용하였으며, 후처리과정에서는 음절의 핵 사이에 위치할 수 있는 음소의 수는 제한된다는 선험적인 지식을 이용하였다. 실험결과, 제안하는 방법을 이용했을 때의 삽입오류는 맹목 세그먼테이션에 비해 약 25% 감소하였다.

• 디지털융복합연구
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• 제8권1호
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• pp.11-26
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• 2010

본 연구는 모바일 콘텐츠 서비스에 대한 신뢰성 구축을 위하여 기술과 조직(공급자)의 역할에 대한 프래임웍을 고찰하는데 목적이 있다. 관광은 지역관광객 유치를 통하여 지역의 경제활성화에 중요한 정책대상으로 빠르게 시장이 성장하고 있다. 또한 인턴넷 확산에 따른 다양한 관광콘텐츠 서비스가 개발되고 있고 최근 유비쿼터스 환경에 적합한 관광콘텐츠 서비스가 개발되고 있다. 본 연구에서는 지방정부가 지역의 다양한 관광자원과 관광숙박시설, 음식점 등을 모바일 디바이스를 통해 서비스 추진시 지속적인 신뢰성을 확보하기 위하여 초기와 지속화 단계별로 기술과 운영조직간의 역할을 표적집단면접법(focus group interview)을 통해 변수를 도출하고 중요도를 분석하였다. 연구결과에서 모바일 콘텐츠 서비스의 지속적 신뢰성 구축을 위하여 초기단계에서는 기술 신뢰성에 대한 중요성이 높게 나타났으며, 지속적 신뢰를 위해서는 관광부문에 참여하고 있는 다양한 공급자들의 역할이 중요한 것으로 분석되었고, 이에 따른 모바일콘텐츠 서비스 신뢰성 유지를 위한 기술과 조직간에 역할의 변화가 필요한 것으로 볼 수 있다. 본 연구는 모바일 서비스 신뢰성 구축을 위하여 유비쿼터스 환경에 적합한 콘텐츠 서비스 개발시 기술개발과 운영조직간의 역할 변화를 제시하였다.

• 한국지능시스템학회논문지
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• 제20권5호
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• pp.645-651
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• 2010

최근 지능형 범죄가 늘면서 첨단 보안 기술에 대한 요구가 점차 늘어나고 있다. 현재까지 보고된 위조영상검출방법은 실용화를 위하여 정확도 개선이 요구된다. 본 논문에서는 사람의 얼굴에 대하여 동공의 반사광을 이용한 얼굴위조판별 시스템을 제안한다. 제안된 시스템은 먼저 다중 스케일 가버특징 벡터를 기반으로 눈의 위치를 찾은 후 2단계의 템플릿 매칭을 통해서 설정된 적용범위를 벗어나는 눈에 대하여 위조판별을 고려하지 않음으로써 정확도를 높이는 방법을 사용한다. 신뢰도가 확보된 눈의 위치를 기반으로 적외선 조명에 반사되는 동공의 특징을 이용하여 눈위치 근처에서의 화소값을 계산하여 위조 여부를 판단한다. 실험을 통하여 본 논문에서 제안한 방법이 더욱 신뢰성 높은 위조판별시스템임을 확인하였다.