• 치과방사선
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• 제24권2호
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• pp.327-333
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• 1994

The purpose of this study was to investigate the irradiation effects on the palatal mucosa. For this study, Sprague-Dawley strain rats were irradiated to their head and neck region with the dose of 5Gy and l0Gy by 6MV X -radiation and sacrificed on the experimental periods after irradiation. The authors observed the histological changes of the hard and soft palatal mucosae. The results were as follows: In the light microscopic examination, hydropic change on the basal cells, increased cell size of the epithelium, and decreased epithelial cell layers were observed on the 3hours, 6hours, and 12hours groups after irradiation. But, basal cell hyperplasia, increased epithelial cell layers, and elongated rate pegs were observed on the 3days group after irradiation. After then, these changes were recovered in the mucosa of the hard palate on the 7days and 14days groups, and in the mucosa of the soft palate on the 14days and 2&lays groups after irradiation. And such changes were greater in the mucosa of the soft palate than in that of the hard palate, and more prominent in l0Gy irradiated groups than in 5Gy irradiated groups.

• Applied Microscopy
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• 제25권1호
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• pp.75-85
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• 1995

The goal of the present study is to investigate the precise variation of tubulin substances in the cytoplasm of oviductal ciliated cells and the morphological changes in cytoplasmic organelles of ciliated cells for ciliogenesis by estrous cycles. The animals used in this study were female rat (Sprague Dawley strain), weighing approximately 200 gm. The ampulla oviducts of these animals (at each of estrous cycle) were rapidly excised. At each stage of estrous cycle, the tissues were used for immunocytochemical study and other were used for electron microscopical study. All specimens were observed by the light and electron microscope. The results obtained are as follows: 1. In the ciliated cells at proestrus, Golgi complex showed $5{\sim}7$ stacked cisternae with numerous saccules and vacuoles. Large amount of fibrous granules were located near the Golgi complex. But at metestrus and diestrus, few fibrous granules were seen. 2. A moderate number of rough endoplasmic reticulum and polyribosomes were scattered in the cytoplasm of ciliated cells at proestrus, but were decrease in number at metestrus and diestrus. 3. At proestrus and estrus, there were a large amount of vesicles in the apical cytoplasm of ciliated cells. 4. Numerous mitochondria were located in the apical cytoplasm at proestrus and estrus, but only a few at metestrus and diestrus. 5. At proestrus and estrus, tubulin substances showed strong reactions in the cytoplasm but weak reactions at metestrus and diestrus. It is suggested consequently that the ciliated cells of the rat oviducts showed no morphological changes of cilia but the ultrastructural organelles of the cells were changed in its shape and location during the entire estrous cycle.

• 한국식품영양학회지
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• 제4권1호
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• pp.21-34
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• 1991

The purposes of this studies were to investigate the effect of dietry Se levels and alcohol administration on the lipid hyperoxidation and the lipid metabolism in the rat. Seventy two male rats of Sprague-Dawley Strain weighting about 58~629 were divided into 12groups. The dietary Se levels were 10, 0.4 and Omg, and the dietary a-tocopherol levels were 150 and 0mg per kg diet, respectively. Alcohol-administrated groups received drinking water solution containing 10% of ethanol from the 3-weeks of experimental periods. The obtained experimental results are summarized as follows. 1. Food intake, body weight gain and food efficiency ratio were significantly lower in H-, L-and alcohol administrated groups(-A) by administrated Se and alcohol in diet. The weight of liver and spleen tended to be greater in H- and alcohol administrated groups. 2. The glutathione values in liver tend to be lower in alcohol or Vit. E administrated groups than nonadministrated groups. Also there were higher in H- and L- than C-groups, but the increasing range decreased due to administrated alcohol. The lipid peroxide values In liver were significantly higher in alcohol groups, and L- and tocopherol groups were higher values. Specially the increasing of lipid peroxide values were significantly effected by alcohol in low Se and Vit. E groups. 3. The contents of total glyceride in plasma were higher in alcohol groups, there were significantly higher values in alcohol administrated groups under low Se and Vit. E groups. The contents of total cholesterol and HDL-cholesterol In plasma were significantly higher in alcohol groups. 4. The contents of total lipid in liver were higher alcohol groups, and slightly higher values in low Se groups(L-groups) than other groups, also higher values in low Vit. E groups. Those of total glyceride in liver were significantly higher in alcohol groups, appeared highest values when alcohol was administrated in low Se and Vit. E groups. The increasing of total glyceride content was significantly effected by alcohol in low Se groups than that in C-groups.

• 한국식품영양학회지
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• 제9권4호
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• pp.472-477
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• 1996

간장해에 미치는 녹각의 효과를 밝힐 목적으로 녹각을 성분별로 추출 분리하여 급여하고 galactosamine으로 간장해를 유도한 후 녹각추출물이 간기능 유지에 관여하는 효소활성 및 지질 대사에 미치는 영향을 생화학적 측면에서 고찰하였다. Cytochrome P-450은 녹각추출물 급여로 증가하였으며 갈락토사민 투여로 감소하였으나 neutral-ext.군에서 높은 증가를 보였다. Glutathione peroxidase 활성은 갈락토사민 투여로 감소하였으나, 녹각 추출물 급여로 활성이 증가하였다. 간조직의 글루타티올 함량은 녹각추출물 급여로 유의적 변동이 없었으며 갈락토사민 투여로 감소하였다. 과산화지질 함량은 대조군에 비해 각각의 녹각추출물 급여군에서 감소하여 특히 water-ext. 군에서 감소정도가 가장 현저하였다. 간조직중의 총 지방질, 총 콜레스테롤 및 트리글리세리드의 함량은 대조군에 비해 각각의 녹각추출물 급여군에서 감소하였으며, water-ext.군에서 가장 많이 하게 감소하였다. 이상의 결과로 미루어 볼 때 녹각의 water-ext.과 neutral-ext.에 함유되어 있는 성분이 간기능 유지에 관여하는 효소합성 증가를 통해 활성증가를 유도하고, 이로써 독성의 대사를 촉진시키므로 간독성을 예방할 수 있을 것으로 생각되며, 이와 같은 녹각추출물의 작용이 생체에 흡수되어진 xenobiotics의 해독작용과 연관될 것으로 생각된다.

• 한국동물학회지
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• 제33권3호
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• pp.297-302
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• 1990

본 연구는 인삼 사포닌이 수영으로 훈련된 웅성 흰 쥐의 혈청 alanine aminotransferase 활성에 미치는 영향을 연구하였다. 훈련군은 50일간 수영훈련시켰으며, 인삼군은 체중 Kg 당 150mg의 인삼 사포닌을 2주간 구강 투여하였다. 인삼 사포닌을 투여한 훈련군은 수영훈련 종료일 직전 2주간에 걸쳐 체중 Kg 당 150mg의 인삼 사포닌을 구강 투여하였다. 훈련군의 혈청 ALT 활성과 인삼군의 혈청 ALT 활성은 대조군 보다 각각 약 72.89%와 57.14%로 유의성 있게 증가하였다. 실험군인 인삼 투여 훈련군과 인삼 비투어 훈련군의 혈청 ALT 활성은 대조군인 비투여 및 비훈련군 보다 각각 79.31% 및 69.66%로 유의성 있게 증가하였다. 인삼 투여훈련군을 인삼 비투어 훈련군과 비교하여 본다면 인삼 사포닌의 영향은 무의성으로 나타났다. 따라서 훈련 및 인삼 사포닌은 각각 흰 쥐의 혈청 ALT 활성을 뚜렷하게 증가시키나, 인삼 투여 훈련군의 경우 인삼 사포닌은 훈련으로 인해 증가된 흰 쥐의 혈청 ALT 활성을 더욱 상승시키지는 않는 것으로 생각된다.

• 한국응용과학기술학회지
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• 제9권1호
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• pp.15-23
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• 1992

This study was aimed to observe dose-response effects of squid liver oil enriched in n3 polyunsaturated fatty acids(n-3 PUFA) on the metabolism of streptozotocint(STZ)-induced diabetic rats. In this experiment, 24 STZ-induced diabetic male rats of Sprague Dawley strain were divided into 4 groups and fed for 4 weeks with basal diet(0%). 33%,67% and 100% squid liver oil(SLO) of total fat content, and 6 normal rats were fed with 0% SLO diet at the same time. The results obtained were as follows: 1. Rat group fed with 33% SLO diet showed the least body weight loss and changes in blood glucose, while ones of 100% SLO diet showed the highest level. 2. Serum total protein and ratio of albumin to globulin of all the groups were below the standard level, but did not show significantly different among diet groups. 3. Serum creatinine concentration of all the groups were stayed whthin normal range, but BUN were 3 to 4 times higher than normal rats. BUN concentration of rats fed with 0% and 33% SLO diet was significantly lower than those of others. 4. Total-cholesterol level of serum increased in all the groups except 33% SLO diet, but since HDL-chol, levels and TG concentration went up with an incerase of SLO in the diets, the ratio of HDL-chol. to total-chol, of rats fed with 67% and 100% SLO diet showed higher than those with 0%, and 33% SLO, and TG concentration of rats fed with 67% and 100% SLO diet decreased significantly.

• 한국식품영양과학회지
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• 제14권2호
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• pp.123-130
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• 1985

This study was designed to observe the nutritional effects of mugwort powder in albino rats. Forty eight young male albino rats, Sprague-Dawley strain, body weight of $80{\pm}3g$ were employed in the study. They were fed on the basal diets supplemented with 0,2,4,6, and 10% mugwort powder for 4 weeks respectively. The amounts of food and protein intake in animal groups of 4 to 8% mugwder supplemented were higher than that of control group. The efficiencies of food and protein in animal groups of mugwort powder supplemented were lower than that of nonsupplemented group. The animal group of 4% mugwort powder supplemented group was highest in energy intake among of other groups. The growing rate of animal groups of 2-6% mugwort powder supplemented groups were similar to that of the control group where as 8% or more of mugwort powder supplemented groups were below than the control group. The contents of hemoglobin and hematocrit were no remarkable difference among of the all groups. The levels of blood sugar in the groups of 8% and 10% mugwort powder supplemented were significantly higher than that of the control group, but their A/G ratio of the serum protein were significantly lower than that of the control group instead.

• 한국식품영양과학회지
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• 제14권2호
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• pp.131-136
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• 1985

This study was intended to observe some nutritional effects of the boiling water extracts of mugwort powder in albino rats. Forty young male albino rats of Sprague-Dawley strain, body weight of $77{\pm}5g$ were employed for the experiment. They were divided into 5 dietary group, 0 (control), 1, 2, 4, and 8g% mugwort powder extract supplemented into the control diet, and fed each corresponding diet to the rats for 4 weeks. They were observed on intake of food and protein, efficiencies of food and protein, amount of energy intake, growing rate, and levels of hematocrit and hemoglobin in the blood. The amount of intakes of food, protein and energy was the highest in the group fed on 4g% mugwort extracts supplemented diet than any other groups studied. The efficiencies of food and protein in the groups fed on mugwort extracts supplemented diets were all lower than that of the control group. The growing rate of the group fed on 1g% mugwort extracts supplemented diet was the highest, however, there was no significant difference among them. The levels of hemoglobin of the groups fed 4g% or 8g% mugwort extracts supplemented diet was higher than that of the control, but not significant.

• 약학회지
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• 제56권4호
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• pp.222-229
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• 2012

In order to determine acetylcholine and choline in the biological samples, the specific enzymes of acetylcholinesterase (AChE) and choline oxidase (ChO), which utilize acetylcholine and choline as substrates, were employed to convert substrates to $H_2O_2$. The produced $H_2O_2$ was coupled to 4-aminoantipyrine/phenol with peroxidase (PO) yielding quinoneimine dye which was measured at 508 nm. In the present enzymatic spectrophotometric analysis the product at the equilibrium state was measured considering accuracy, precision, time and cost of the analysis. The developed analytical method yielded good linearity (calibration curve; $A_{508}$=9534[acetylcholine]+0.009, correlation coefficient ($R^2$); 0.999) with detection limit of $1.11{\times}10^{-7}M$, reasonable precision (relative standard deviation; 0.10~1.62% at $2.5{\times}10^{-6}M{\sim}1.25{\times}10^{-4}M$) and accuracy (relative error; -0.24~0.97% at $4.13{\times}10^{-6}M{\sim}1.01{\times}10^{-4}M$) for acetylcholine chloride standard solution. The concentrations of acetylcholine and choline in human serum were found as $3.20{\times}10^{-5}M$ and $1.14{\times}10^{-4}M$, respectively. The brain tissues of Sprague-Dawley strain rat contained 9.82${\mu}g/g$ of acetylcholine and 6.53 ${\mu}g/g$ of choline in the cerebrum, while 7.37 ${\mu}g/g$ of acetylcholine and 5.34 ${\mu}g/g$ of choline in the cerebellum.

• Imaging Science in Dentistry
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• 제37권2호
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• pp.69-77
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• 2007

Purpose: To evaluate the accuracy of the imaging reformation of cone beam computed tomography for the assessment of bone defect healing in rat model. Materials and Methods: Sprague-Dawley strain rats weighing about 350 gms were selected. Then critical size bone defects were done at parietal bone with implantation of collagen sponge. The rats were divided into seven groups of 3 days, 1 week, 2 weeks, 3 weeks, 4 weeks, 6 weeks, and 8 weeks. The healing of surgical defect was assessed by multi planar reconstruction (MPR) images and three-dimensional (3-D) images of cone beam computed tomography, compared with soft X-ray radiograph and histopathologic examination. Results: MPR images and 3-D images showed similar reformation of the healing amount at 3 days, 1 week, 2 weeks, and 8 weeks, however, lower reformation at 3 weeks, 4 weeks, and 6 weeks. According to imaging-based methodologies, MPR image revealed similar reformation of the healing amount than 3-D images compare with soft X-ray image. Among the four threshold values for 3-D images, 400-500 HU revealed similar reformation of the healing amount. Histopathologic examination confirmed the newly formed trabeculation correspond with imaging-based methologies. Conclusion: MPR images revealed higher accuracy of the imaging reformation of cone beam computed tomography and cone beam computed tomography is a clinically useful diagnostic tool for the assessment of bone defect healing.