• 수산해양기술연구
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• 제51권2호
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• pp.265-271
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• 2015

The feeding habits of Okamejei kenojei were studied using 242 specimens collected from October 2010 to September 2011 in the coastal waters of Gadeok-do, Korea. O. kenojei was carnivorous feeder that consumed mainly shrimps, crabs, and fishes. Its diet also included small quantities of stomatods, cephalopods, amphipods. The size of O. kenojei ranged from 10.4 to 47.4 cm in total length (TL). O. kenojei showed ontogenetic dietary shift. <15.0, 15.0-19.9 cm size classes fed mainly on shrimps and amphipods. 20.0-24.9 cm size class fed mainly shrimps and crabs. Thereafter, shrimps and crabs were decreased with ontogenesis while fishes and cephalopods were increased. Increasing size, the mN/ST were constantly decreased, but mW/ST were constantly increased.

• Tuberculosis and Respiratory Diseases
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• 제59권4호
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• pp.406-412
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• 2005

연구배경 : 최근 말초혈액을 결핵균 특이 항원으로 자극후 분비되는 $IFN-{\gamma}$ 를 측정하는 $QuantiFERON^{(R)}-TB$ Gold (QFT) 와 T $SPOT-TB^{(R)}$(SPOT) 검사가 임상에서 잠복결핵의 진단에 상품화되어 사용되고 있다. 그러나 이전에 시행한 투베르쿨린 검사가 상기 체외 $IFN-{\gamma}$ 검사의 위양성을 유발할 수 있는 가능성에 대해서는 아직 발표된 자료가 없다. 방 법 : 과거 결핵치료력 또는 환자 접촉력이 없는 46명의 15세 남자 고등학생을 대상으로 하였다. 혈액을 채취하여 $IFN-{\gamma}$ 검사를 시행하고, 이단계 TST를 시행하였다. 첫 번째 검사에서 $IFN-{\gamma}$ 검사와 TST가 모두 음성인 학생을 대상으로 하여 2개월 째에 TST, 2개월, 4개월 째에 QFT, 그리고 2개월 째에 SPOT 검사가 반복 시행되었다. 결 과 : TST 경결 크기의 평균값은 $3.1{\pm}5.4mm$ (범위: 0-20)였고, 이단계 TST에서 최종 13/46명(28.3%)이 양성 결과를 나타내었다. SPOT검사는 9명(19.6%)에서 양성이었으며 QFT는 1명(2.2%)에서만 양성이었다. 첫 $IFN-{\gamma}$ 검사와 TST에서 모두 음성인 학생 24명에서 2개월에 시행한 TST 모두 음성이었다. 2개월(23명) 및 4개월(25명)에 시행한 QFT 검사는 모두 음성이었다. 2개월에 SPOT 검사를 시행한 23명 중 1명(4.3%)에서 약양성 결과를 보였다. 결 론 : 두 가지 체외 $IFN-{\gamma}$ 검사의 결과에 일부 차이가 있었으나 2개월 및 4개월 이전에 시행된 TST가 체외 $IFN-{\gamma}$ 검사결과에 영향을 미치지 않음을 알 수 있었다.

• 한국산학기술학회논문지
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• 제11권9호
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• pp.3155-3160
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• 2010

최근 자동차 산업은 고효율 자동차의 개발에 박차를 가하고 있으며 이에 따라 차체 경량화 및 내식성 방지를 위한 고강도강 및 도금강의 사용이 증가하고 있다. 이에 따라 본 연구에서는 자동차 차체에 쓰이는 3종의 다른 강판에 대하여, 겹침 순서에 따른 용접 특성을 시뮬레이션 기법을 이용하여 분석하였다. 자동차 차체에 많이 사용되는 EDDQ 급의 도금강판 0.7t와 인장강도 440 MPa급의 440R 1.2t, 그리고 AHSS (Advanced High Strength Steel)인 DP 590 0.2t 판재에 대하여 겹치기 순서에 따른 용접 특성을 시뮬레이션 하였고, 너겟 성장 곡선 및 전류에 따른 너겟의 크기로 그 용접성을 분석하였다. 또한 용접 시 발생되는 각 접촉부의 접촉저항을 이용하여 겹침순서에 따른 너겟의 크기를 분석하였으며, 이를 통해 최적의 겹치기 순서를 제안할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.620-627
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• 2004

On the foundation of a database of genome sequences and protein analyses, the ability to clone a gene based on a peptide analysis is becoming more feasible and effective for identifying a specific gene and its protein product of interest. As such, the current study conducted a protein analysis using 2-D PAGE followed by MALDI- TOF and ESI-MS to identify a highly expressed gene product of C. parasitica. A distinctive and highly expressed protein spot with a molecular size of 47.2 kDa was randomly selected and MALDI-TOF MS analysis was conducted. A homology search indicated that the protein appeared to be a fungal enolase (enol). Meanwhile, multiple alignments of fungal enolases revealed a conserved amino acid sequence, from which degenerated primers were designed. A screening of the genomic $\lambda$ library of C. parasitica, using the PCR amplicon as a probe, was conducted to obtain the full-length gene, while RT-PCR was performed for the cDNA. The E. coli-expressed eno 1 exhibited enolase enzymatic activity, indicating that the cloned gene encoded the C. parasitica enolase. Moreover, ESI-MS of two of the separated peptides resolved from the protein spot on 2-D PAGE revealed sequences identical to the deduced sequences, suggesting that the cloned gene indeed encoded the resolved protein spot. Northern blot analysis indicated a consistent accumulation of an eno1 transcript during the cultivation.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.215-220
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• 2000

Abstract White spot disease (WSD), resulting in more than 90% mortality of aquacultured penaeid shrimp, has been reported off the southern and western coasts of Korea since 1993. The pafuogen of WSD has been identified as being a virion wifu an envelope around a central nucleocapsid, and with an average size of 167 nm in diameter and 375 nm in length. In the present study, an in situ hybridization technique was developed as a rapid. sensitive, and specific diagnostic assay for the WSD viros infection in shrimp. Furthermore. the pathological changes ofWSD, in shrimp experimentally infected with WSD viroses. were investigated. Using a biotinylated 643 bp probe obtained from a peR using primers specific to the rod-shaped virus of Penaeus japonicus (RV-PJ), positive signals were detected in both naturally and experimentally infected shrimps. The in situ hybridization revealed positive reactions in the nuclei of the stromal matrix cells in the lymphoid organ, epithelia of the gills, foregut. epidermis, and hematopoietic cells of the interstitial tissues, suggesting the presence of WSD virus. Tills result indicates that the in situ hybridization method can be useful for a rapid and sensitive detection of WSD viruses in shrimp.shrimp.

• Bulletin of the Korean Chemical Society
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• 제31권8호
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• pp.2293-2298
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• 2010

Focused electrospray (FES) deposition method is presented for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. FES ion optics consists of two cylindrical focusing electrodes capped with a truncated conical electrode through which an electrospray emitter passes along the cylindrical axis. A spray of charged droplets is focused onto a sample well on a MALDI target plate under atmospheric pressure. The shape and size distributions of matrix crystals are visualized by scanning electron microscope and the mass spectra are obtained by time-of-flight mass spectrometry. Angiotensin II, bradykinin, and substance P are used as test samples, while $\alpha$-cyano-4-hydroxycinnamic acid and dihydroxybenzoic acid are employed as matrices. FES of a sample/matrix mixture produces fine crystal grains on a 1-3 mm spot and reproducibly yields the mass spectra with little shot-to-shot and spot-to-spot variations. Although FES greatly stabilizes the signals, the space charge due to matrix ions limits the detection sensitivity of peptides. To avoid the space charge problem, we adopted a dual FES/FES mode, which separately deposits matrix and sample by FES in sequence. The dual FES/FES mode reaches the detection sensitivity of 0.88 amol, enabling ultrasensitive of peptides by homogeneously depositing matrix and sample under atmospheric pressure.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권3호
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• pp.1633-1649
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• 2017

In remote sensing image processing, the traditional fusion algorithm is based on the Intensity-Hue-Saturation (IHS) transformation. This method does not take into account the texture or spectrum information, spatial resolution and statistical information of the photos adequately, which leads to spectrum distortion of the image. Although traditional solutions in such application combine manifold methods, the fusion procedure is rather complicated and not suitable for practical operation. In this paper, an improved IHS transformation fusion algorithm based on the local variance weighting scheme is proposed for remote sensing images. In our proposal, firstly, the local variance of the SPOT (which comes from French "Systeme Probatoire d'Observation dela Tarre" and means "earth observing system") image is calculated by using different sliding windows. The optimal window size is then selected with the images being normalized with the optimal window local variance. Secondly, the power exponent is chosen as the mapping function, and the local variance is used to obtain the weight of the I component and match SPOT images. Then we obtain the I' component with the weight, the I component and the matched SPOT images. Finally, the final fusion image is obtained by the inverse Intensity-Hue-Saturation transformation of the I', H and S components. The proposed algorithm has been tested and compared with some other image fusion methods well known in the literature. Simulation result indicates that the proposed algorithm could obtain a superior fused image based on quantitative fusion evaluation indices.

• 한국화재소방학회논문지
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• 제25권4호
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• pp.48-55
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• 2011

차동식 스포트형 공기 팽창식 열감지기를 인공 가속열화 시켰을 때 발생하는 고장 원인에 대하여 분석하였다. 감지기에 고장이 발생하는 원인은 감열실 금속을 고정하는 접합재(binder)가 플라스틱 몰딩(moulding)에서 분리되어 발생하는 고장과, 감열실 금속과 다이어프램 금속을 고정하는 플라스틱 몰딩에 균열이 발생되는 고장으로 확인되었다. 고장이 발생하지 않은 감지기는 감열실 금속 및 다이어프램 금속이 플라스틱 몰딩에 접합재로 완전히 부착되어 있었다. 또한 2010G 감지기는 접합재의 기계적 강도를 향상시키기 위하여 접합재에 유리강화섬유가 추가되었음이 확인되었다. 감지기 종류별에 따른 플라스틱과 접합재의 밀도에는 큰 차이가 없었다. 그러나 감지기의 플라스틱에 대한 열중량분석(TGA) 결과, 제작년도는 같지만 제조회사가 다른 2005A와 2005B의 플라스틱 열적특성이 동일하지 않은 것으로 확인되었다.

• 한국어류학회지
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• 제32권3호
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• pp.143-147
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• 2020

2019년 1월부터 12월까지 서해5도 주변해역에서 채집된 홍어 379개체의 위내용물 조성을 조사하였다. 홍어의 체반장은 5.9~34.5 cm의 범위를 보였고, 새우류가 가장 우점한 먹이생물 분류군으로 나타났으며, 그 다음으로 어류의 비율이 높았고, 그 외 집게류, 갑각류, 두족류 등이 낮은 비율로 섭식되었다. 새우류의 비율은 하계에서 가장 낮은 비율을 보였고, 동계에서는 높았으며, 어류는 하계에서는 높았고, 동계에서는 낮게 나타났다.

• 한국기계가공학회지
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• 제20권12호
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• pp.24-29
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• 2021

Recently, owing to the high demand for eco-friendly cars in the automotive industry, noise and vibrations have become major challenges. The use of laminated damping steel is increasing in response to these demands. Laminated damping steel is primarily used in sound insulation plates. The vibration energy is converted into thermal energy due to the viscoelastic resin being located between two steel sheets and being able to damp the vibrations when an external force, such as, noise or vibration is applied to the steel plate. Laminated damping steel is chiefly applied to dash panels in automotive body parts, and because of its structure, junction technology for bonding with other components is necessary. However, there has not been sufficient research conducted on junctions. In this study, regardless of the electrode shape, in the range of 4.0 ~ 8.0 kA welding current, the same welding force and welding time were applied which were 2.8 kN and 200 m/s (12 cycles) and the tensile shear load and nugget size were analyzed after the resistance spot welding between different materials of laminated damping steel with a thickness of 1.035 mm. The results show that in the range of 5 ~ 8 kA welding current, 1.035 mm laminated damping steel meets the MS181-15 standard, which is the technical standard of Hyundai-Kia Motors.