Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Focused Electrospray Deposition for Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

  • Jeong, Kyung-Hwan (Bio-Nanotechnology Center, Department of Chemistry, Pohang University of Science and Technology) ;
  • Seo, Jong-Cheol (Bio-Nanotechnology Center, Department of Chemistry, Pohang University of Science and Technology) ;
  • Yoon, Hye-Joo (Bio-Nanotechnology Center, Department of Chemistry, Pohang University of Science and Technology) ;
  • Shin, Seung-Koo (Bio-Nanotechnology Center, Department of Chemistry, Pohang University of Science and Technology)
  • Received : 2010.05.16
  • Accepted : 2010.06.26
  • Published : 2010.08.20
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Abstract

Focused electrospray (FES) deposition method is presented for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. FES ion optics consists of two cylindrical focusing electrodes capped with a truncated conical electrode through which an electrospray emitter passes along the cylindrical axis. A spray of charged droplets is focused onto a sample well on a MALDI target plate under atmospheric pressure. The shape and size distributions of matrix crystals are visualized by scanning electron microscope and the mass spectra are obtained by time-of-flight mass spectrometry. Angiotensin II, bradykinin, and substance P are used as test samples, while $\alpha$-cyano-4-hydroxycinnamic acid and dihydroxybenzoic acid are employed as matrices. FES of a sample/matrix mixture produces fine crystal grains on a 1-3 mm spot and reproducibly yields the mass spectra with little shot-to-shot and spot-to-spot variations. Although FES greatly stabilizes the signals, the space charge due to matrix ions limits the detection sensitivity of peptides. To avoid the space charge problem, we adopted a dual FES/FES mode, which separately deposits matrix and sample by FES in sequence. The dual FES/FES mode reaches the detection sensitivity of 0.88 amol, enabling ultrasensitive of peptides by homogeneously depositing matrix and sample under atmospheric pressure.

Keywords

References

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