• Title/Summary/Keyword: Spot Detection

검색결과 265건 처리시간 0.031초

상호연관 신경망에 기반을 둔 이동 검출을 위한 아날로그 집적회로 (Analog MOS circuits for motion detection based on correlation neural networks)

  • 심선일;김용태;박정호
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2000년도 추계종합학술대회 논문집(3)
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    • pp.149-152
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    • 2000
  • We propose simple analog MOS circuits producing the one-dimensional compact motion-sensing circuits. In the proposed circuit, the optical flow is computed by a number of local motion sensors which are based on biological motion detectors. Mimicking the structure of biological motion detectors made the circuit structure quite simple, compared with conventional velocity sensing circuits. Extensive simulation results by a simulation program of integrated circuit emphasis (SPICE) indicated that the proposed circuits could compute local velocities of a moving light spot and showed direction selectivity for the moving spot

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Proteomic identification of the bovine pregnancy associated proteins by peptide mass fingerprinting

  • Kang, Sun-Chul;Kim, Eun-Jung;Pyo, Jae-Hoon;Lim, Jin-Kyu
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2002년도 생물공학의 동향 (X)
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    • pp.558-561
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    • 2002
  • In this study, a method for the localization of pregnancy-specific proteins from cow urine on 2-D gel have been established. The proteins were digested with trypsin in gel and then analyzed with MALDI- TOF or transferred to a membrane and microsequenced. To examine the pregnancy associated protein spot 2 as a diagnosis marker in bovine urine 2-D western blotting was performed. This antibody was reacted specifically in the protein of pregnant cow’s urine. Consequently spot 2 was identified and found to be good candidates for developing cow pregnancy detection assay kit.

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자동차 차체 제조 공정에서 용접 공정 오류 검출을 위한 지능형 모니터링 시스템 개발 (Development of Intelligent Monitoring System for Welding Process Faults Detection in Auto Body Assembly)

  • 김태형;유지영;이세헌;박영환
    • Journal of Welding and Joining
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    • 제28권4호
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    • pp.81-86
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    • 2010
  • In resistance spot welding, regardless of the optimal condition, bad weld quality was still produced due to complicated manufacturing processes such as electrode wear, misalignment between the electrode and workpiece, poor part fit-up, and etc.. Therefore, the goal of this study was to measure the process signal which contains weld quality information, and to develop the process fault monitoring system. Welding force signal obtained through variety experimental conditions was analyzed and divided into three categories: good, shunt, and poor fit-up group. And then a monitoring algorithm made up of an artificial neural network that could estimate the process fault of each different category based on pattern was developed.

박층크로마토그라피 및 농도 측정법에 의한 Ampicillin Trihydrate 중의 N,N-Dimethylaniline의 검색 (Detection of N,N-Dimethylaniline in Ampicillin Trihydrate by Thin Layer Chromatography and Densitometry)

  • 이왕규;김박광;심창구
    • 약학회지
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    • 제22권4호
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    • pp.238-241
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    • 1978
  • A simple and convenient method of detecting N, N-dimethy laniline (D.M.A.) residues in ampicillin trihydrate (A.T.) was established. D.M.A. was extracted by chloroform from the chloroform presaturated N/10-ammonia water solution of A.T. and chromatographed on silica gel G thin layer. Blue spot appeared in 15minutes after spray of 2, 6-dichloroquinonechlorimide solution was compared with the blue spot of reference concomitantly processed. The developing solvent was prepared by mixing equal volume of cyclohexane and chloroform. To quantitate the amount of D.M.A. in A.T., T.L.C. was performed with the Eastmann Chromatogram sheet, then color density was measured by Cosmo Superclick densitometer. The developing solvent at this time was cyclohexane-chlorofonn (3+7) mixture. The peak areas obtained with the amount of D.M.A ranging from 0.05 to 2.0 .mu.g were linear to color density. Better sensitive results would be available with the densitometer equipped with monochromator.

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열감지장치의 응답시간지수와 경계구역 (Installed Spacing and Reponse Time Index of Heat Detection Devices)

  • 권오승;이복영;김동석
    • 한국화재소방학회:학술대회논문집
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    • 한국화재소방학회 1996년도 학술발표회
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    • pp.72-75
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    • 1996
  • The objectives of this paper investigate the effect of installed spacing on the activation of spot type heat detection devices. The flow of hot gases under a ceiling resulting from the impingement of a fire plume activates heat detectors and sprinklers. Local temperature and velocity in this ceiling jet are usually expressed with the function of a ceiling height, the distance from a fire location and the heat release rate of fire. And detectors having different. RTI respond in different ways to the same temperature and velocity of ceiling jet. Thus great care should be taken to decide installed spacing of heat detection devices by considering above effects.

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철도건널목 지장물 영상검지장치 개발 (Development of Obstacle Detection System on a Railroad Crossing)

  • 조봉관;류상환
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2009년도 제40회 하계학술대회
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    • pp.1197_1198
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    • 2009
  • The development of Technology study for preventing of accident and reducing the risk through the intelligence of level crossing is provide the detection of stopped car at railway crossing with the most advanced intelligence technology such as sensor, computer, communication and date processing and transmit to the operational staff on broad for reaction or make the train stopped automatically through the connection with train. Also this study include that showing the situation of crossing railway when the train is approached and prevent the accident and reduce the risk through the connection of road transit signal system. On this study is performed the test through the date from spot level crossing and the development of video detection algorism for stopped road transit vehicle at level crossing with intelligent system.

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극박 냉연강판의 경사진 핀홀 검출에 관한 연구 (The Measurement of The Inclined Pinhole in The Cold Strip)

  • 김하술;배호문;이희준
    • 한국소성가공학회:학술대회논문집
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    • 한국소성가공학회 1999년도 제3회 압연심포지엄 논문집 압연기술의 미래개척 (Exploitation of Future Rolling Technologies)
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    • pp.201-207
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    • 1999
  • The automatic pinhole detection system is described. The goal of this project is to study the feasibility test of the new concept for hole detection. The developed method is able to detect almost 50$\mu\textrm{m}$ pinhole by evaluating the shining of the light as if there is pinhole in the strip. Moreover, it is possible to inspect up to the 200$\mu\textrm{m}$ inclined pinhole. The system cosists of three main functional parts: the source part of the light which is using the linear halogen lamp, the image gathering part which is using a line CCD and the image processing part. The light spot can be controlled and optimized corresponding to the situation of the strip. To eliminate back ground noise, the binary image processing method is adopted.

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Development of Novel Method for the Detection of Microcystin Using Chemiluminescence Immunochromatography

  • Pyo, Dong-Jin;Yoo, Ji-Sun
    • Bulletin of the Korean Chemical Society
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    • 제32권1호
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    • pp.149-152
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    • 2011
  • A new chemiluminescence immunochromatographic analysis system with high sensitivity and high reproducibility was developed for the determination of microcystins (MCs) in water. Horse radish peroxidase (HRP) labeled microcystin monoclonal antibody was used for the sensitive chemiluminescence detection. The chemiluminescence immunochromatographic analysis system was composed of microcystin LR (MCLR)-monoclonal antibody (mAb)-Horse Radish Peroxidase (HRP) conjugate, MCLR-BSA conjugate, luminol, hydrogen peroxide mixture solution, an immunochromatographic assay strip and luminometer. To detect the concentration of microcystins in water, we utilized one spot analysis of the strip instead of flow type analysis. We could detect the microcystins in water at a concentration as low as 9.45 pg/mL with the chemiluminescence (CL) detection.

원자력 압력용기의 피복하부 결함검출에 대한 고찰(II) (A Study of the Detection for Underclad Cracks of Nuclear Pressure Vessel)

  • 박치승;강기원
    • 비파괴검사학회지
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    • 제13권2호
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    • pp.31-39
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    • 1993
  • 국내 원자력 압력 용기의 피복하부 결함에 대한 검사는 1970년대 초부터 이 피복하부 결함에 대한 보고가 되기 시작하였으나 적용 규격인 ASME Code의 요구사항이 아니므로 현재까지는 검사를 수행해 오지 않고 있다. 본 실험의 목적은 이러한 피복하부 결함을 검출하는데 적절한 초음파검사 방법의 조건을 찾는 것이다. 실험은 $70^{\circ}$굴절종파, 50/70 multibeam탐촉자, SLIC-50 탐촉자 등을 사용하여 원자력 압력용기의 피복 용접과 같은 조건하에서 피복용접 시킨 초음파보정 시험편과 demonstration시험편에 대하여 수행하였다. 실험 결과 피복하부 결함의 검출은 50/70 multibeam탐촉자가 효과적이었으면, 피복하부 결함의 길이 평가는 $70^{\circ}$굴절종파로 수행하는 것이 바람직한 것으로 나타났으며, 반면에 피복하부 결함의 깊이 측정은 SLIC-50 탐촉자를 사용하여 M-SPOT 방범과 M-PET 방법으로 평가하는 것이 가장 효과적인 것으로 사료된다.

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Improvement of RT-PCR Sensitivity for Fruit Tree Viruses by Small-scale dsRNA Extraction and Sodium Sulfite

  • Lee, Sin-Ho;Kim, Hyun-Ran;Kim, Jae-Hyun;Kim, Jeong-Soo
    • The Plant Pathology Journal
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    • 제20권2호
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    • pp.142-146
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    • 2004
  • Woody plant tissues contain great amounts of phenolic compounds and polysaccharides. These substances inhibit the activation of reverse transcriptase and/or Taq polymerase in RT-PCR. The commonly used multiple-step protocols using several additives to diminish polyphenolic compounds during nucleic acid extraction are time consuming and laborious. In this study, sodium sulfite was evaluated as an additive for nucleic acid extraction from woody plants and the efficiency of RT-PCR assay of commercial nucleic acid extraction kits and small-scale dsRNA extraction was compared. Sodium sulfite was used as an inhibitor against polyphenolic oxidases and its effects were compared in RNA extraction by commercial extraction kit and small-scale double-stranded RNA (dsRNA) extraction method for RT-PCR. During nucleic acid extraction, addition of 0.5%-1.5%(w/v) of sodium sulfite to lysis buffer or STE buffer resulted in lighter browning by oxidation than extracts without sodium sulfite and improved the RT-PCR detection. When commercial RNA extraction kit was used, optimal concentrations of sodium sulfite were variable according to the tested plant. However, with dsRNA as RT-PCR template, sodium sulfite 1.5% in STE buffer improved the detection efficiency of Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in fruit trees, and reduced the unspecific amplifications signi-ficantly. Furthermore, when viruses existed at low titers in host plant, small-scale dsRNA extractions were very reliable.