• BMB Reports
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• 제37권6호
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• pp.720-725
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• 2004

A number of signaling molecules contain small pleckstrin homology (PH) domains capable of binding phosphoinositides or proteins. Phospholipase C (PLC)-${\gamma}1$ has two putative PH domains, an $NH_2$-terminal (PH1) and a split PH domain ($nPH_2$ and $cPH_2$). We previously reported that the split PH domain of PLC-${\gamma}1$ binds to phosphatidylinositol 4-phosphate (PI(4)P) and phosphatidylinositol 4,5-bisphosphate (PI(4,5)$P_2$) (Chang et al., 2002). To identify the amino acid residues responsible for binding with PI(4)P and PI(4,5)$P_2$, we used site-directed mutagenesis to replace each amino acid in the variable loop-1 (VL-1) region of the PLC-${\gamma}1$ $nPH_2$ domain with alanine (a neutral amino acid). The phosphoinositide-binding affinity of these mutant molecules was analyzed by Dot-blot assay followed by ECL detection. We found that two PLC-${\gamma}1$ nPH2 domain mutants, P500A and H503A, showed reduced affinities for phosphoinositide binding. Furthermore, these mutant PLC-${\gamma}1$ molecules showed reduced PI(4,5)$P_2$ hydrolysis. Using green fluorescent protein (GFP) fusion protein system, we showed that both $PH_1$ and $nPH_2$ domains are responsible for membrane-targeted translocation of PLC-${\gamma}1$ upon serum stimulation. Together, our data reveal that the amino acid residues $Pro^{500}$ and $His^{503}$ are critical for binding of PLC-${\gamma}1$ to one of its substrates, PI(4,5)$P_2$ in the membrane.

• 한국언론정보학보
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• 제27권
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• pp.283-316
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• 2004

본 연구는 국책사업인 핵폐기장 유치와 관련해 지역간 경쟁과정에서 나타난 갈등적 이슈들을 해당 지역신문들이 어떻게 다루고 있는지를 뉴스프레임 분석을 통해 고찰했다. 연구대상인 광주일보, 매일신문, 전북일보의 뉴스기사 분석결과, 핵폐기장이라는 같은 사건에 대해 각각의 신문들이 사용하는 주요 프레임이 상이하게 나타났다. 우선 광주일보와 매일신문에서는 대항 프레임이 높게 사용되었으며, 이와 달리 전북일보에서는 지역발전 프레임이 가장 높게 집계되었다. 또한 헤드라인 주제어와 뉴스프레임 구성은 깊은 연관성이 있는 것으로 밝혀졌다. 광주일보와 매일신문의 대항프레임 기사에서는 헤드라인 주제어로 핵폐기장 사용 빈도가 높았으며, 지역발전 프레임의 전북일보기사에서는 헤드라인 주제어로 방사성폐기장이 압도적으로 높게 나타났다. 정부의 '양성자'와 '핵폐기장' 연계정책 발표 이후에도 광주일보와 매일신문에서의 뉴스 프레임은 그 이전과 비교하여 변화가 거의 없었다. 이에 반해 전북일보에서는 연계 정책 발표 이후, 대항 프레임이 현격히 감소한 대신에 정책 프레임은 크게 증가한 것으로 집계되었다. 동시에 전북일보의 헤드라인 주제어 사용에서 핵폐기장은 급격하게 감소하였으며, 방사성폐기장은 급격하게 증가한 것으로 나타났다. 이러한 결과에서 전북일보는 헤드라인 주제어로 방사성폐기장을 즐겨 사용하여 핵폐기장의 부정적 이미지를 최소화하고, 핵폐기장 유치가 곧 지역발전이라는 여론을 조성한 것이라 할 수 있다. 언론이 구성한 세계가 현실 그 자체가 된다는 그간의 프레임 연구 결과들은 당시 전북 부안으로 핵폐기장 유치가 결정된 것은 결코 우연이 아님을 보여주고 있다.

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1291-1299
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• 2007

Two ${\beta}$-1,4-glucanases (DI and DIII fractions) were purified to homogeneity from the culture filtrate of a cellulolytic bacteria, Cellulomonas sp. CS 1-1, which was classified as a novel species belonging to Cellulomonas uda based on chemotaxanomic and phylogenetic analyses. The molecular mass was estimated as 50,000 Da and 52,000 Da for DI and DIII, respectively. Moreover, DIII was identified as a glycoprotein with a pI of 3.8, and DI was identified as a non-glycoprotein with a pI of 5.3. When comparing the ratio of the CMC-saccharifying activity and CMC-liquefying activity, DI exhibited a steep slope, characteristic of an endoglucanase, whereas DIII exhibited a low slope, characteristic of an exoglucanase. The substrate specificity of the purified enzymes revealed that DI efficiently hydrolyzed CMC as well as xylan, whereas DIII exhibited a high activity on microcrystalline celluloses, such as Sigmacells. A comparison of the hydrolysis patterns for pNP-glucosides (DP 2-5) using an HPLC analysis demonstrated that the halosidic bond 3 from the nonreducing end was the preferential cleavage site for DI, whereas bond 2, from which the cellobiose unit is split off, was the preferential cleavage site for DIII. The partial N-terminal amino acid sequences for the purified enzymes were $^1Ala-Gly-Ser-Thr-Leu-Gln-Ala-Ala-Ala-Ser-Glu-Ser-Gly-Arg-Tyr^{15}$-for DI and $^1Ala-Asp-Ser-Asp-Phe-Asn-Leu-Tyr-Val-Ala-Glu-Asn-Ala-Met-Lys^{15}$-for DIII. The apparent sequences exhibited high sequence similarities with other bacterial ${\beta}$-1,4-glucanases as well as ${\beta}$-1,4-xylanases.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제35권4호
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• pp.248-255
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• 2013

Reconstruction techniques of orofacial defects caused by wide excision of the intraoral malignant lesions are various. Although radial forearm free flap is a common donor site on reconstruction of soft tissue defect, anterolateral thigh (ALT) free flap also has an established site in orofacial soft tissue reconstruction as the favored donor flap with recent progress of the microsurgical technique. A 59-year-old female complained of hyperplastic mass on the right retromolar and buccal cheek, which was diagnosed as a squamous cell carcinoma (SCC) by an incisional biopsy. Before the operation, we planned a wide excision of the SCC lesion, supraomohyoid neck dissection, reconstruction with radial forearm free flap (RFFF), and split thickness skin graft. We accidentally found an arterial variation of the forearm area during elevation of RFFF, and changed the plan of reconstruction operation to reconstruction with ALT free flap. Operative sites was healed well during the post-operative period, and we referred to the department of radiation oncology for post-operative radiotherapy.

• 아동학회지
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• 제7권1호
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• pp.1-23
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• 1986

The present study investigated the current child care practices of professional working mothers in Korea and their needs for substitute child care outside the home. Three hundred twenty-two professional working mothers who have children under 6 years of age in Seoul, Pusan, Taegu, and Taejon were surveyed with the use of a questionnaire. It was found that during their working hours, most of the professional working mothers put their children in the care of either the paternal or the maternal grandmothers. Others put the children in the care of a full-time housekeeper or a part-time housekeeper. While all the mothers had worked out a way to care for their children, almost all of them would use day care if facilities were available. Specifically, 42 per cent prefer child care at their work site, 21 per cent prefer family day care, and only 3 per cent indicated that they would not use any child care facilities. Most of the working mothers think that their employer should manage the day care program. Others prefer social agency or government management. Regarding costs, most prefer that costs should be split 50-50 between the employer and the family. In addition, professional working mothers wanted quality programs with educational components rather than mere custodial care. It was concluded that variety in types of quality child care programs should be available to provide working mothers the options of choosing what they want for their children. The main type of programs suggested by this study is work site based child care for children under 3 years of age. The existing law for the Saemaul Nursery School program should be implemented so as to include programs for children under 3.

• Archives of Plastic Surgery
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• 제39권4호
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• pp.345-351
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• 2012

Background Cranial base defects are challenging to reconstruct without serious complications. Although free tissue transfer has been used widely and efficiently, it still has the limitation of requiring a long operation time along with the burden of microanastomosis and donor site morbidity. We propose using a reverse temporalis muscle flap and calvarial bone graft as an alternative option to a free flap for anterior cranial base reconstruction. Methods Between April 2009 and February 2012, cranial base reconstructions using an autologous calvarial split bone graft combined with a reverse temporalis muscle flap were performed in five patients. Medical records were retrospectively analyzed and postoperative computed tomography scans, magnetic resonance imaging, and angiography findings were examined to evaluate graft survival and flap viability. Results The mean follow-up period was 11.8 months and the mean operation time for reconstruction was $8.4{\pm}3.36$ hours. The defects involved the anterior cranial base, including the orbital roof and the frontal and ethmoidal sinus. All reconstructions were successful. Viable flap vascularity and bone survival were observed. There were no serious complications except for acceptable donor site depressions, which were easily corrected with minor procedures. Conclusions The reverse temporalis muscle flap could provide sufficient bulkiness to fill dead space and sufficient vascularity to endure infection. The calvarial bone graft provides a rigid framework, which is critical for maintaining the cranial base structure. Combined anterior cranial base reconstruction with a reverse temporalis muscle flap and calvarial bone graft could be a viable alternative to free tissue transfer.

• Archives of Plastic Surgery
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• 제36권6호
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• pp.720-726
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• 2009

Purpose: Fasciocutaneous flap with random pattern flap has limitation in mobility and length - width ratio. This characteristic is more pronounced in lower extremity which has relatively poor vascularity. Perforator based flap in lower extremity reconstruction has various advantages as a axial flap, allowing abundant blood supply and widening of mobility range. So if it is not a case of wide defect, free flap can be replaced by perforator based flap. Methods: From April 2007 to March 2009, 18 cases of perforator flap were performed. 8 had defect in upper 1/3 of calf, 6 in middle 1/3, and 4 in lower 1/3. In 10 cases island flap were used, 3 case had transposition flap, 2 cases used advancement flap, 2 case had propeller flap and 1 case had rotation flap. Results: 17 cases survived without flap necrosis. Partial flap necrosis occurred in 1 case, so secondary split thickness skin graft was done. Chronic wound with pseudomonas infection occurred in 1 case, but it was completely cured with conservative treatment. Conclusion: Perforator based flap is useful in lower extremity reconstruction because of relative freedom in changing the size and thickness of the flap depending on the recipient site, good mobility, and abundant vascularity. And donor site morbidity can be minimized. Lower extremity reconstruction using perforator based flap is a good method because it can minimize the complication and obtain effective result.

• Imaging Science in Dentistry
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• 제32권4호
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• pp.201-206
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• 2002

Purpose : To evaluate the radiographic change of operation sites after orthognathic surgery using the digital image processing and fractal analysis. Materials and Methods : A series of panoramic radiographs of thirty-five randomly selected patients who had undergone mandibular orthognathic surgery (bilateral sagittal split ramus osteotomy) without clinical complication for osseous healing, were taken. The panoramic radiographs of each selected patient were taken at pre-operation (stage 0), 1 or 2 days after operation (stage 1), 1 month after operation (stage 2), 6 months after operation (stage 3), and 12 months after operation (stage 4). The radiographs were digitized at 600 dpi, 8 bit, and 256 gray levels. The region of interest, centered on the bony gap area of the operation site, was selected and the fractal dimension was calculated by using the tile-counting method. The mean values and standard deviations of fractal dimension for each stage were calculated and the differences among stage 0, 1, 2, 3, and 4 were evaluated through repeated measures of the ANOVA and paired t-test. Results : The mean values and standard deviations of the fractal dimensions obtained from stage 0, 1, 2, 3, and 4 were 1.658±0.048, 1.580±0.050, 1.607±0.046, 1.624±0.049, and 1.641 ±0.061, respectively. The fractal dimensions from stage 1 to stage 4 were shown to have a tendency to increase (p < 0.05). Conclusion: The tendency of the fractal dimesion to increase relative to healing time may be a useful means of evaluating post-operative bony healing of the osteotomy site.

• 교육시설 논문지
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• 제22권1호
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• pp.13-23
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• 2015

Currently, the group study becomes the most popular method and common custom for learning in public that is distinctly different from the preferred way of self-study in the last decades. As a result, the college and public facilities have been significantly changed to provide more and more new spaces for discussion, learning and sharing information with others as one of the remarkable improvements and advantages. On the other hands, library is one of the all-important public sites to obtain information and knowledge for students. Moreover, we can split out an individual space from the reading rooms to take part in the group activities such as group meetings and academic exchanges. Recently, several universities begin to recognize the value of group study and try to meet the research needs. Also, needs for students, and the group study rooms are applied into the new buildings and rebuilt ones under this background. In this study, It focuses on analyzing of the 134 group study rooms to investigate the variations in universities and classify the types of buildings with site researches through 14 indicators of indoor environments. To investigate different types of group study rooms, we use the SD method to analyze the findings. So far there is no research focusing on the study rooms, especially for the analysis of their types and indoor environments features. Therefore, this article can provide a theoretical basis and evidence to related researches; also can help us to improve indoor environments to offer a better learning environments for the students in the future.

• 해양환경안전학회지
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• 제21권5호
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• pp.492-500
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• 2015

본 논문에서는 새만금 개발사업이 해양환경에 미치는 영향에 대한 국민들의 인식을 분석하였다. 이를 위해 새만금 인근지역인 전북 군산시, 김제시, 부안군 400가구 및 새만금 인근지역 외 전국 600가구 등 총 1,000가구를 대상으로 설문조사를 수행하여 얻은 결과를 분석하였다. 또한 새만금 개발사업의 경제적 효과 및 해양환경 영향을 동시에 고려할 때 새만금 개발사업에 대해 판단하는 공공의 태도에 영향을 미치는 요인을 분석하기 위해 서열프로빗 모형을 적용한 통계적 분석을 수행하였다. 인식조사 결과, 새만금 개발사업의 경제적 효과와 해양환경 영향을 동시에 고려할 때, 새만금 인근지역조사 및 전국조사 응답자의 70 % 이상이 새만금 개발사업을 반대하였다. 또한 설문조사 응답자의 90 % 이상이 새만금 방조제 외해역의 해양환경관리가 필요하다고 인식하고 있었다. 따라서 새만금 방조제 외해역 환경관리를 위한 적절한 대책이 마련되어야 할 것이다.