• Proceedings of the Korean Aquaculture Society Conference
• /
• 2003.10a
• /
• pp.52-52
• /
• 2003

For the evaluation of hormonal control of spermiation in fish, a method to quanify the spermiation response or mature Rhynchocypris oxycephalus (Sauvage and Dabry) to hormonal therapy is described. Spermatocrit was determined after 7 min centrifugation at 18,000 $\times$ｇ and sperm density was estimated by a standard hemocytormer method. Sperm density can be predicted from spermatocrit since their relationship is linear as described by the regression equation, Y=3.68X-27,18 ($R^2$=0.82, N=50), where Y is spermatocrit and X is sperm density. Milt production by mature R. oxycephalus was highest at 24 h after injection of 1,000 IU human chorionic gonadotropin (HCG) and 50 $\mu\textrm{g}$ luteinizing hormone-releasing hormone analogue (LHRHa) per kg body weight. Increased milt production coincided with low spermotocrit and sperm density levels. These results demonstrate that spermition in mature R. oxycephalus can be reliably evaluated by a spermatocrit method and that HCG and LHRHa are effecove in stimulating of spermiation in this species.

• Development and Reproduction
• /
• v.19 no.4
• /
• pp.253-258
• /
• 2015

Spermiation was stimulated in the mature grass puffer, Takifugu niphobles, with an injection of human chorionic gonadotropin (HCG) or carp pituitary extract (CPE). Spermatocrit and sperm density were reduced, but milt production was increased in both the HCG and CPE treatment groups relative to those in the control group (P < 0.05). These results should be useful for increasing the fertilization efficiency in grass puffer breeding programs.

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 1998.07a
• /
• pp.68-69
• /
• 1998

정액의 ml당 정자수는 $1.13 \pm 0.34 \times 10^{10}$이었고 spermatocrit는 $64.8 \pm 1.4$였으며, 정장의 삼투질농도는 $266 \pm 2$ mOsm/kg이었다. 총 단백질 함량과 총 지질 함량은 정장에 비해 정자에서 높은 값을 보였고, glucose는 검출되지 않았다. 황복 정자를 16일간 $0 \pm 0.5 \circ C$에서 냉장보존 하였을 때, 수정률은 0-0.7%로 보존효과가 저조한 것으로 나타났다. 황복 정자의 냉동보존을 위한 희석액으로는 marine fish Ringer's solution, 동해방지제로는 5% DMSO가 적합하였으며, 이때의 수정률은 $79.3 \pm 7.5$%였다.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.32 no.3
• /
• pp.243-246
• /
• 1999

To obtain the basic data for the preservation of river puffer (Takifugu obscurus) sperm, experiments were carried out on the physico-chemical properties and cold storage of milt. The average number of sperm and spermatocrit in milt stripped were $1.13\pm0.34\times10^{10}/ml$ and 64.8$\pm$1.4, respectively. Osmolality of seminal fluid was 266$\pm$2 mOsm/kg, Total protein and total lipid from sperm were higer than that from seminal fluid. $Ca^{2+}$ and $Na^{+}$ concentrations were higher in the seminal fluid than in the sperm, while $Mg^{2+}$ and $K^{+}$ concentrations were lower in the seminal fluid. When sperm of river puffer were preserved in $0\pm0.5^{\circ}C$ with various diluents for 16 days, fertilization rate was $0\~0.7\%$. It suggested that cold storage of river puffer sperm was detrimental to sperm fertility.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.32 no.2
• /
• pp.238-241
• /
• 1999

Experiments were performed to find out the physico-chemical properties of milt and the sperm motilities in various conditions using the grey mullet, Mugil cephalus. The average concentration of sperm in the milt was $1,11 \pm0.36\times10^{10}/ml$. Spermatocrit was 96.7$\pm$2.6. pH and osmolality of seminal fluid were 7.8$\pm$0.1, 370$\pm$6 mOsm/kg, respectively, Total protein concentration of sperm was higher than that of seminal fluid, but total lipid concentration of seminal fluid was higher than that of sperm. The sperm motility was high in the diluent of milt : artificial seawater (1:10, by volume) and in 822 mOsm/kg and 983 mOsm/kg similar to seawater osmolality, but it decreased after 20 minutes. But activity of sperm was highly maintained in 482 mOsm/kg which was a little higher than osmolality of seminal fluid, and was high in pH 7$\~$9.

• Journal of Aquaculture
• /
• v.19 no.1
• /
• pp.47-51
• /
• 2006

Experiments were performed to find out the physico-chemical properties of milt and sperm motility in various storage conditions using starry flounder (Platichthys stellatus). The average sperm concentration and spermatocrit in stripped milt were $6.00{\pm}0.98{\times}10^8/mL\;and\;72{\pm}5$, respectively. The osmolality and pH were $337{\pm}9mmol/kg$ and $7.7{\pm}0.1$, respectively. The sperm of starry flounder was preserved with artificial seminal plasma (ASP), Stein's solution (SS) and marine fish Ringer's solution (MFRS) at $0^{\circ}C,\;2^{\circ}C,\;and\;4^{\circ}C$. The most effective condition for cold storage was SS at $0^{\circ}C$, and the preserved sperm remained motile for 30 days.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.29 no.4
• /
• pp.474-480
• /
• 1996

Physicochemical changes of milt during the spermiation period were investigated in black seabream (Acanthopagrus schlegeli) reared in recirculating seawater system. The spermiation period for the milt collection in cultured brood stock was from 11 April to 4 June. During the spermiation period, average milt volume (ml/100 g body weight) was $0.70{\pm}0.33\;ml$ and maintained high level from 2 May to 4 June. The total number of stripping spermatozoa per 100 g body weight reached the maximum value $(3.32{\times}10^{10})$ in 9 May, then decreased rapidly thereafter. Spermatozoa concentration per ml reached the minimum value in 2 May. There was no change in spermatocrit for the spermiation period. Total protein, total lipid, glucose and Na concentration in spermatozoa and seminal fluid were lower than those in plasma. Total protein, total lipid and K concentration in spermatozoa were higher than those in seminal fluid. The glucose concentration in spermatozoa and seminal fluid in April and May were significantly higher than those in June.

• Development and Reproduction
• /
• v.11 no.3
• /
• pp.227-233
• /
• 2007

The milt properties of filefish(Thamnaconus modestus) included physical properties of sperm and biochemical properties of seminal plasma. The physical properties of milt were $0.3{\pm}0.1\;mL{\cdot}fish^{-1}$ in sperm volume, $2.6{\pm}0.1{\times}10^7\;spermatozoa{\cdotg}mL^{-1}$ in sperm concentration and $73.3{\pm}6.7$ in spermatocrit. The biochemical properties of seminal plasma contained $9.8{\pm}0.9\;mmol{\cdot}L^{-1}$ potassium, $164.0{\pm}4.0\;mmol{\cdot}L^{-1}$ sodium, $151.0{\pm}1.2\;mmol{\cdot}L^{-1}$ chloride, $14.9{\pm}0.6\;mg{\cdot}dL^{-1}$ calcium, $7.2{\pm}0.1\;mg{\cdot}dL^{-1}$ magnesium, $1.0\;mg{\cdot}dL^{-1}$ glucose, $0.1\;g{\cdot}dL^{-1}$ total protein and $1.0\;mg{\cdot}dL^{-1}$ total lipid. The osmolality and pH of seminal plasma were $322.8{\pm}2.8\;mOsmol{\cdot}kg^{-1}$ and $7.7{\pm}0.1$, respectively. The spermatozoon of filefish consisted of three parts: head without acrosome, mid-piece with five mitochondria and flagellum with "9+2" pattern. The head of spermatozoon in longitudinal section was horseshoe-shaped, and $1.3{\sim}1.6\;{\mu}m$ long and $1.0{\sim}1.3\;{\mu}m$ wide.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.31 no.3
• /
• pp.353-358
• /
• 1998

Experiments were performed to find out the physico-chemical properties of milt, and morphological changes of cryopreserved spermatozoa in tiger puffer, Takifugu rubripes. The average number of sperm and spermatocrit in milt stripped were $9.81{\pm}0.34{\times}10^{10}/m{\ell}$ and $97.8{\pm}0.8$, respectively. While total lipid concentration from seminal fluid was higher than that from sperm, total protein concentration from sperm was higher than that from seminal fluid, Na and K concentrations in sperm and those in seminal fluid were similar each other, However, glucose from sperm and seminal fluid were not detectable. Spermatozoon of tiger puffer was consisted of head, middle Piece and tail. Size of head showing horseshoe shape was $0.65{\pm}0.10{\mu}m$ in diameter and $1.35{\pm}0.30{\mu}m$ in length. The head fully containing chromatin did not have acrosome. Mitochondrion in middle piece was $0.2{\mu}m$ in average diameter and flagellum showed 9+2 structure. A few of cryopreserved spermatozoa showed morphologically loose or swollen plasma membranes.

• Journal of Fisheries and Marine Sciences Education
• /
• v.28 no.2
• /
• pp.596-602
• /
• 2016

Experiments were carried out examine short-term effects of human chorionic gonadotropin (hCG) and long-term effects of luteinising hormone releasing hormone analogue (LHRHa) on milt production of the tongue sole Cynoglossus semilaevis. In the first experiment, each fish was implanted with a blank cholesterol pellet (control), 100 and $200{\mu}g$ LHRHa per kg body weight. In the second experiment, fish were injected with either 100, 200, 400 and 800 IU hCG per kg body weight or same volume of marine fish Ringer's solution. In the first experiment, milt volume was increased in male implanted with $200{\mu}g$ LHRHa pellet compared with other groups at day 10. Injection of 400 and 800 IU hCG resulted in an increase in the milt volume at hour 96 after the treatment. Although statistical difference is unable to confirm because of small milt volume, compared with the control group, hormone pellet-treated groups had a reduction in the mean spermatocrit (Sct) and sperm concentration (Sc). The results suggest that the increase in milt volume is at least partially gonadotropin (GtH)-dependent and increased milt volume has a relationship with milt hydration.