Proceedings of the Korean Aquaculture Society Conference (한국양식학회:학술대회논문집)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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The Effect of Exogenous Hormone Treatment on Spermiation in Rhynchocypris oxycephalus (Sauvage and Dabry)

  • Park, In-Seok (Division of Ocean Science, College of Ocean Science and Technology, Korea Maritime University) ;
  • Hur, Jun-Wook (Research Institute of Marine Science and Technology, Korea Maritime University) ;
  • Park, Geyong-Cheol (Chungbuk Inland fisheries Institute) ;
  • Nam, Yoon-Kwon (Department of Aquaculture, College of Fisheries Sciences, Pukyong National University) ;
  • Kim, Dong-Soo (Department of Aquaculture, College of Fisheries Sciences, Pukyong National University)
  • Published : 2003.10.01
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Abstract

For the evaluation of hormonal control of spermiation in fish, a method to quanify the spermiation response or mature Rhynchocypris oxycephalus (Sauvage and Dabry) to hormonal therapy is described. Spermatocrit was determined after 7 min centrifugation at 18,000 $\times$ｇ and sperm density was estimated by a standard hemocytormer method. Sperm density can be predicted from spermatocrit since their relationship is linear as described by the regression equation, Y=3.68X-27,18 ($R^2$=0.82, N=50), where Y is spermatocrit and X is sperm density. Milt production by mature R. oxycephalus was highest at 24 h after injection of 1,000 IU human chorionic gonadotropin (HCG) and 50 $\mu\textrm{g}$ luteinizing hormone-releasing hormone analogue (LHRHa) per kg body weight. Increased milt production coincided with low spermotocrit and sperm density levels. These results demonstrate that spermition in mature R. oxycephalus can be reliably evaluated by a spermatocrit method and that HCG and LHRHa are effecove in stimulating of spermiation in this species.

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