• Title/Summary/Keyword: Spectrophotometric analysis

Search Result 190, Processing Time 0.026 seconds

Analysis of Absorbance for Tinted Dye Absorbed into Tinted Lenses by Spectrophotometric Method (분광광도법에 의한 칼라 렌즈 내 염색제에 대한 흡광도 분석)

  • Choi, Eun-Jung;Jung, Ju-Hyun
    • Journal of the Korean Chemical Society
    • /
    • v.53 no.6
    • /
    • pp.704-708
    • /
    • 2009
  • We studied absorbance of tinted dye absorbed into tinted lenses by Spectrophotometric method. Used lenses and tinted dye were CR-39 plastic optical lenses and Ons & $Lavas^{(R)}$ Blue, respectively. Absorbance values of tinted dye absorbed into tinted lenses were obtained from the differences of absorbance values between tinted lenses and CR-30 lenses. Average concentrations of tinted dye absorbed into tinted lenses were obtained from the mass differences between tinted lenses and CR-30 lenses. The relationship between absorbance and average concentration for tinted dye absorbed into tinted lenses was also confirmed to follow Beer’s law. The absorption coefficient calculated from the tangent of the calibration curve was determined to be a=0.983 $Lg^{-1}cm^{-1}$.

Spectrophotometric Determination of Bisphenol A by Complexation with Ferricyanide and Ferric chloride solution (Ferricyanide와 ferric chloride 혼합액을 사용한 Bisphenol A의 비색 정량법 개발)

  • Kum, Eun-Joo;Ryu, Hee-Young;Kwon, Gi-Seok;Sohn, Ho-Yong
    • Journal of Life Science
    • /
    • v.17 no.2 s.82
    • /
    • pp.266-271
    • /
    • 2007
  • Bisphenol A (BPA) has been widely used as a monomer for production of epoxy resins and polycarbonate plastics. The annual production of BPA exceeds 640,000 metric tons in worldwide. BPA, a suspected phenolic endocrine disrupter, is moderately soluble and frequently detected in industrial wastewater. To date, HPLC and GC has been used for BPA analysis. However, HPLC and GC-analysis need high operation lost, experts, and an elaborate pre-treatment of samples, and is difficult to apply on-time and mass analysis. Therefore, simple, mass and rapid detection of BPA in environments is necessary. In the present study, spectrophotometric method of BPA quantification was developed. Based on blue-color product formation with BPA and ferric chloride/ferricyanide under the optimized conditions, the standard curve was acquired $({\lambda}_{750}=0.061\;BPA\;[{\mu}M]+0.07155,\;R^2=0.992)$. Using an established method, the BPA contents in the soil extract, and different water samples and living products, including disposable syringe, cup and plastic tube, were analyzed. The results suggested that the method is useful for BPA determination from different massive samples. Since the BPA metabolites, nontoxic 4-hydroxyacetophenone or 4-hydroxybenzaldehyde, did not form blue-color product, this method is also useful to screen a microorganism for BPA bioremediation.

Spectrophotometric Determination of Soil Chemical Properties Using Soiltek® KA-P Spectrophotometer (Soiltek KA-P 분광광도계률 사용한 토양 화학적 성질의 분광학적 분석)

  • Hyun, Hae-Nam;Oh, Sang-Sil;Koo, Bon-Jun;Kang, Ho-Jun
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.33 no.2
    • /
    • pp.127-138
    • /
    • 2000
  • To enable rapid and convenient soil test, new soil analytical methods, which require only one instrument, UV/Vis spectrophotometer, were developed and named "Soiltek KA-P spectrophotometric methods". The Soiltek$^{(R)}$ KA-P spectrophotometric method was compared with standard method of RDA in analytical capability for soil chemical properties. Using the 78 soils collected from upland, paddy, orchard, and vinyl house soils, soil organic matter, exchangeable K, Ca, and Mg. CEC, available $SiO_2$, and nitrate were analyzed by the two methods. The color stability(ratio of the absorbance at elapsed time t to the absorbance at time t=0) of organic matter. Ca, Mg, and available $SiO_2$ decreased to about 2% within one hour. However, that of exchangeable K, CEC, and nitrate remained constant. The results obtained with Soiltek$^{(R)}$ KA-P spectrophotometric method showed highly significant correlation with those measured by the standard method of RDA($R^2$ >0.9501), in which the slopes were near unity of $1.0{\pm}0.05$. The standard deviation values of organic matter, exchangeable K, Ca, and Mg, CEC, available $SiO_2$, and nitrate were apparently lower than ${\pm}1.8gkg^{-1}$, ${\pm}0.05cmol^+kg^{-1}$, ${\pm}0.18cmol^+kg^{-1}$, and ${\pm}0.13cmol^+kg^{-1}$, ${\pm}1.0cmol^+kg^{-1}$, ${\pm}5.0mgkg^{-1}$, and ${\pm}10.0mgkg^{-1}$, respectively. All the measurements showed coefficients of variation of less than 7~17% and were within the confidence level of 95%, which means both the methods are precise. Considering the relative simplicity, low cost, precision and accuracy, the proposed Soiltek$^{(R)}$ KA-P spectrophotometric methods could be recommended as an alternative to standard method.

  • PDF

Spectrophotometric Determination of Lanthanide Ions by Flow Injection Analysis (Flow Injection Analysis에 의한 란탄족 이온들의 흡광광도 정량)

  • Kang, Sam-Woo;Cho, Kwang-Hee
    • Analytical Science and Technology
    • /
    • v.9 no.3
    • /
    • pp.244-252
    • /
    • 1996
  • Spectrophotometric properties of lanthanide complexes with methylthymol blue(MTB) and cetyltrimethylammonium bromide(CTAB) were studied and also lanthanide(III) ions were determined by flow injection analysis on the base of the above results. The absorption maxima of lanthanide(III)-MTB complexes in the presence of CTAB are 635nm with molar absorptivity of $4.51{\sim}6.11{\times}10^4Lmol^{-1}cm^{-l}$ at pH 5.8. The mole ratio of lanthanide(III) complexes with MTB is 1:2 in the presence of CTAB. The calibration curves of lanthanide(III) ions obey the Beer's law in the range of 0.1 to 0.4ppm under the optimum condition. The samples throughput was ca. $60hr^{-1}$. The interfering effect of some cations and anions was investigated. The ligand anions such as tartrate and citrate, many transition and rare earth elements interfered severely and must be removed before the determination of lanthanide(III) ions.

  • PDF

Double Ion-Pair Formation in Aqueous Solutions of Methylene Blue and Tetraphenylborate

  • Yoon, Kil-Joong;Kim, Kang-Jin
    • Bulletin of the Korean Chemical Society
    • /
    • v.6 no.3
    • /
    • pp.149-152
    • /
    • 1985
  • Spectrophotometric and spectrofluorimetric methods of analysis were conducted in dilute aqueous solutions of methylene blue and tetraphenylborate. The formation of double ion-pair was confirmed and its overall formation constant, ${\Delta}H^{\circ}$, and ${\Delta}S^{\circ}$ were obtained. The irradiation with wavelengths over visible region resulted in bleaching the mixed solution. The bleaching reaction was also proceeded at $55^{\circ}C$. The reactions of the double ion-pair were briefly discussed.

Kinetic Analysis of Cathepsin B Inhibitor Using a Spectrophotometric Assay (분광광도법에 의한 Cathepsin B 저해물질의 효소동력학적 저해특성 조사)

  • 한길환;김상달
    • Microbiology and Biotechnology Letters
    • /
    • v.29 no.2
    • /
    • pp.90-95
    • /
    • 2001
  • Kinetic Analysis of Cathepsin B Inhibitor Using a Spectrophotometric Assay. Han, Kil-Hwan and SangDal Kim*. Department of Applied MicrobioJ0f5Yt Yeungnam UniversitYt Kyongsan 77 2-749, Korea - The KHS 10, C4Hl10~6 formula produced from Streptomyces luteogriseus KT-] 0 effectively inhibited a lysosomal cysteine proteinase, cathepsin B. It inhibited the enzyme activity of cathepsin B competitively when the N a-CBZ-Llysine p-nitrophenyl ester HC] (CLN) was used as a substrate. The inhibition const:mt (Ki) of KHS 1 0 for cathepsin B detennined by spectrophotometeric assay was 430 nM. The effective inhibition of cathepsin B was observed at $25^{\circ}C$ :md pH 6.0. The cathepsin B inhibitor, KHSlO needed a preincubation of cathepsin B with the inhibitor for over 5 min. The KHS 10 preserved over 80% inhibition activity even after heat-treatment at $100^{\circ}C$ for ] hr.

  • PDF

Fourier Transform Ion Cyclotron Resonance (FT-ICR) MASS Spectrophotometric Analysis of Flower Petal from Paeonia lactiflora cv. ‘Red Charm’ and Evaluation of its Functional Activity (작약 레드참 꽃잎의 이온화원-푸리에 변환 질량분석과 기능성 연구)

  • Kim, June Hyun;Choi, Yong Bock;Lee, Ha Jung;Kim, Yong Hee;Kim, Jun Huan;Sim, Jung Min;Sohn, Young-Sun
    • Korean Journal of Plant Resources
    • /
    • v.29 no.5
    • /
    • pp.588-597
    • /
    • 2016
  • Little attention has been paid to the functional aspect of the flower petal of Paeonia lactiflora, compared to that of its root. To determine the components of flower petal of Paeonia lactiflora, we conducted the Fourier transform ion cyclotron resonance (FT-ICR) MASS spectrophotometric analysis. We detected the 24 different types of ingredients from the 70% ethanol extracts of flower petal of peonia lactiflora cv. ‘Red Charm’. The main compounds were quercetin glucopyranosides, methyl gallate, paonioflolol and kaemperol glucopyranosides. We further tested its functional activity. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of the extracts was 87.9-90.4% at 0.1mg/ml. This result showed that these flower extracts have approximately 5-fold stronger antioxidant potential than a previous report with root extracts (Bang et al. 1999). The result of tyrosinase inhibition assay of Paeonia lactflora extract was almost similar to that of arbutin except significantly higher effect in the coral sunset extract at 0.1% concentration. Hyaluronidase inhibition assay showed 76.5% inhibition at 5% concentration of this flower extract, indicating that Peaonia lactiflora flower extracts have the major anti-inflammatory, anti-oxidant and brightening effects. Taken together, these results suggest these three Paeonia lactiflora species extracts might provide the basis to develop a new natural brightening agent.

Atomic Absorption Spectrophotometric Analysis of Chromium, Nickel and Manganese in Stainless Steel (原子吸光分析에 의한 鋼鐵속에 있는 Cr, Mn 및 Ni 의 定量)

  • Hwang, Jae-Young;Tagliamonti, Josep J.;Parsons, Frank B.
    • Journal of the Korean Chemical Society
    • /
    • v.10 no.3
    • /
    • pp.133-135
    • /
    • 1966
  • Rapid methods for determining chromium, nickel, and manganese in stainless steel by atomic absorption spectrophotometer are described. Use of suitable standards eliminates the need to separate each element prior to analysis. Comparison with the conventional wet chemical analysis shows that atomic absorption spectrophotometer is a reliable analytical tool in these applications.

  • PDF

7Li-NMR and Thermal Analysis for Lithium Inserted into Artificial Carbon Material

  • O, Won Chun
    • Bulletin of the Korean Chemical Society
    • /
    • v.22 no.4
    • /
    • pp.367-371
    • /
    • 2001
  • Lithium inserted into artificial carbon has been synthesized as a function of the Li concentration. The characteristics of these prepared compounds were determined from the studies using X-ray diffraction(XRD), solid nuclear magnetic resonance (NM R) spectrophotometric and differential scanning calorimeter(DSC) analysis. X-ray diffraction showed that lower stage intercalation compounds were formed with increasing Li concentration. In the case of the AG3, most compounds formed were of the stage 1 structure. Pure stage 1 structural defects of artificial graphite were not observed. 7Li-NMR data showed that bands are shifted toward higher frequencies with increasing lithium concentration; this is because non-occupied electron shells of Li increased in charge carrier density. Line widths of the Li inserted carbon compounds decreased slowly because of nonhomogeneous local magnetic order and the random electron spin direction for located Li between graphene layers. The enthalpy and entropy changes of the compounds can be obtained from the differential scanning calorimetric analysis results. From these results, it was found that exothermic and endothermic reactions of lithium inserted into artificial carbon are related to the thermal stability of lithium between artificial carbon graphene layers.

Studies on the Determination of Synthetic Food Colors (합성식용색소의 정량분석법에 관한 검토)

  • Om Ae-Son;Lee Heon-Ok;Shim Jae-Young;Shin Dong-Hwa;Kim Yong-Suk;Lee Young-Hwan;Bang Jeong-Ho;Shin Jae-Wook;Lee Tal-Soo;Hong Ki-Hyoung;Park Sung-Kwan;Choi Duck-Jang;Kim Hee-Yun
    • Journal of Food Hygiene and Safety
    • /
    • v.20 no.2
    • /
    • pp.73-76
    • /
    • 2005
  • Center for Food Standard Evaluation, Korea Food and Drug AdministrationThis study was performed to compare analytical methods of nine synthetic food colors and six food color lakes in Korea, Japan, Joint FAO/WHO Expert Committee of Food Additives (JECFA), and USA. The experimental protocol of this study consists of three parts: titration method with titanium chloride, gravimetric and spectrophotometric method. To measure the total contents of food colors, Korea and Japan used titration method with titanium chloride, USA used the average value of titration method with titanium chloride equipped with Kipp generator and spectrophotometric method. Also, JECFA used titration method with titanium chloride equipped with KiPP generator. However, All the low organizations used gravimetric method to measure the total content of coloring matter on Food Red No.3. Although all organizations use various methods for analysis of coloring matters, total contents of coloring matter on food colors tested fell into the standard showing $85.08-96.40\%$ in synthetic food colors and $10.00-36.86\%$ in food color lakes.