• Toxicological Research
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• 제17권
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• pp.197-199
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• 2001

Enhancement of antigen-specific IgE is a hallmark of allergic hyperresponsiveness, therefore it is necessary to adopt or develop a highly sensitive and specific assay for determination of allergen-specific IgE levels in vivo. In this presentation, we introduce an ELISA (enzyme linked immunosorbent assay) system developed to measure the levels of chicken egg ovalbumin (OVA)-specific IgE in serum. The ELISA method uses a commercially available purified rat anti-mouse IgE as a capture Ab and biotinylated OVA as a detection reagent. Avidin-peroxidase with its substrate is used for color development resulting in optical density measurement at 405 nm. The ELISA system produces a highly sensitive dose-response relation-ship between optical density levels and the dilution titer of the OVA-IgE standard serum but no cross-reaction with unrelated IgE or IgG. It is believed that the system is an Efficient tool to delineate an adjuvant effect of environmental pollutants on development of asthmatic and atopic responses.

• 한국화재소방학회논문지
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• 제20권4호
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• pp.26-32
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• 2006

국내에서는 현재 샌드위치패널의 화재성능 평가를 위하여 KS F 2271(건축물의 내장재료 및 구조의 난연성 시험방법)을 사용하고 있다. 그 중 연기 및 연소독성가스에 대한 시험인 가스유해성 시험은, 건축 재료 및 내장재의 연소시 발생하는 가스의 유해성을 마우스의 평균 행동정지시간으로 측정하는 방법으로, 정량적인 방법이 아니므로 마우스의 상태나 시험조건에 따라 시험결과가 달라질 수 있다. ISO 5659-2 연소챔버 시험방법은 광학밀도를 정량적으로 측정할 수 있으며, FTIR을 이용한 유독가스 분석이 가능하다. 본 연구에서는 ISO 5659-2 연소챔버 시험방법을 사용하여, 국내에서 일반적으로 사용되는 4종의 샌드위치패널 단열재의 광학밀도를 측정하여 각 시험체와 비교하였다. 또한, 시험체마다 3번의 시험 중 두 번째 시험시 FTIR 분석을 수행하여 HCl, $NO_2$ 등의 정량적 결과를 비교하였다.

• 한국화재소방학회:학술대회논문집
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• 한국화재소방학회 2008년도 추계학술논문발표회 논문집
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• pp.450-455
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• 2008

We have carried out the test using the cone calorimeter and the smoke density chamber to evaluate the characteristics of the combustion for the car interior materials passed horizontal burning test. We have analysed many parameters related to fire hazard. These parameters are the ignition time, the heat release rate, the maximum average rate of heat emission, the flashover propensity and specific optical density. There was a significant difference in HRR and optical smoke density. The HRR was $185{\sim}446kW/m^2$ and optical smoke density was $119{\sim}1207$. Only horizontal burning test was performed to evaluate the fire hazard for the car interior materials.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 추계학술대회 논문집 Vol.14 No.1
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• pp.251-254
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• 2001

In this paper, we analyzed the properties change of electric wire when the thermal stress was applied to NFR-8 and FR-PVC 600[V] wire. Messurement is made of the attenuation of a light beam by smoke accumulating with in a closed chamber due to nonflaming pyrolytic decomposition and flaming combustion. Results are expressed in terms of specific optical density which is derived from a geometrical factor and the measured optical density a measurement characteristic of the concentration of smoke. Referenced documents were ASTM E662 standard test method for specific Ds genalated by solid materials. The furnace control system shall maintain the required irradiance level under steady-state condition with the chamber door closed of $2.5{\pm}0.04[w/cm^{2}]$ for 20 min. According to the results of the smoke density analysis of NFR-8 and FR-PVC the highest decomposition flaming smoke density range of NFR-8 and FR-PVC were 25.2 to 37.5 and 51.1 respectively. Nonflaming smoke density range of NFR-8 and FR-PVC were 100.4 to 112.2 and 126.5 to 398.8. The amount of carbon monoxide generated was found to be much higher in FR-PVC decomposition than in NFR-8 due to incomplete combustion of FR-PVC which has high content of carbon in compound.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 추계학술대회 논문집
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• pp.251-254
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• 2001

In this paper, we analysed the properties change of electric wire when the thermal stress was applied to NFR-8 and FR-PVC [600] wire. Measurement is made of the attenuation of a light beam by smoke accumulating with in a closed chamber due to nonflamining pyrolytic decomposition and flaming combustion. Results are expressed in terms of specific optical density which is derived from a geometrical factor and the measured optical density a measurement characteristic of the concentration of smoke. Referenced documents were ASTM E662 standard test method for specific Ds generated by solid materials. The furnace control system shall maintain the required irradiance level under steady-state condition with the chamber door closed of 2.5${\pm}$0.04[w/$\textrm{cm}^2$] for 20 min. According to the results of the smoke density analysis of NFR-8 and FR-PVC the highest decomposition flaming smoke density range of NFR-8 and FR-PVC were 7.2 to 77.5 and 51.1 respectively. Nonflaming smoke density range of NFR-8 and FR-PVC were 100.4 to 112.2 and 126.5 to 398.8. The amount of carbon monoxide generated was found to be much higher in FR-PVC decomposition than in NFR-8 due to incomp1ete combustion of FR-PVC which has high content of carbon in compound.

• 품질경영학회지
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• 제26권4호
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• pp.250-264
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• 1998

The purpose of the thesis is to develop simulation program for forecasting of optical connector. So we can achieve the time and the money saving for making the optical connector. Optical performance (insertion loss) of optical connector mainly relies on 3 misalignment factors-ferrule factor due to mis-manufacture from design, auto-centering effect that is fiber behavior phenomena between hole and fiber, fiber misalignment factor. Simulation use experimental data with auto-centering effect and fiber factor and use pseudo data with ferrule through random number generation because it is developing stage. In this study we a, pp.y kernel density estimation method with experimental data in order to know whether it belong to or not specific parametric distribution family. And we simulate to forecast insertion loss of optical multifiber connector under specific design model using nonparametric bootstrap resampling data and parametric pseudo samples from uniform distribution. We obtain the tolerance specifications of misalignment factors satisfying not exceed in maximum 1.0dB and choose optimal hole diameter.

• 한국화재소방학회논문지
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• 제27권2호
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• pp.75-79
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• 2013

본 연구는 구획화재의 출입구를 통한 연기밀도를 정량적으로 측정하고 측정기법의 신뢰성을 평가하기 위해 불확실성 해석을 수행한다. 메탄 화재 강도에 따른 축소모형 구획공간의 출입구 상층부를 통해 유출되는 연기 유동에 대해 광소멸 측정을 수행하고 Bouguer's의 법칙을 적용하여 연기밀도를 산정하였다. 광투과율, 경로길이, 질량비광소멸상수에 대하여 연기밀도의 측정 불확실도를 평가하였으며 계산된 연기밀도 측정의 확장불확실도는 20% 정도이며 신뢰수준은 95% 이다. 준정상상태의 화재 발열량에 대하여 출입구에서의 평균 연기밀도를 산정하였으며 구획공간내부의 총괄당량비 증가에 따라 출입구의 연기농도가 선형적으로 증가하는 경향을 보였다.

• ALGAE
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• 제31권4호
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• pp.391-401
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• 2016

To develop an easy and rapid method of quantifying lipid contents of marine dinoflagellates, we quantified lipid contents of common dinoflagellate species using a colorimetric method based on the sulpho-phospho-vanillin reaction. In this method, the optical density measured using a spectrophotometer was significantly positively correlated with the known lipid content of a standard oil (Canola oil). When using this method, the lipid content of each of the dinoflagellates Alexandrium minutum, Prorocentrum micans, P. minimum, and Lingulodinium polyedrum was also significantly positively correlated with the optical density and equivalent intensity of color. Thus, when comparing the color intensity or the optical density of a sample of a microalgal species with known color intensities or optical density, the lipid content of the target species could be rapidly quantified. Furthermore, the results of the sensitivity tests showed that only $1-3{\times}10^5cells$ of P. minimum and A. minutum, $10^4cells$ of P. micans, and $10^3cells$ of L. polyedrum (approximately 1-5 mL of dense cultures) were needed to determine the lipid content per cell. When the lipid content per cell of 9 dinoflagellates, a diatom, and a chlorophyte was analyzed using this method, the lipid content per cell of these microalgae, with the exception of the diatom, were significantly positively correlated with cell size, however, volume specific lipid content per cell was negatively correlated with cell size. Thus, this sulpho-phospho-vanillin method is an easy and rapid method of quantifying the lipid content of autotrophic, mixotrophic, and heterotrophic dinoflagellate species.

• Journal of Acupuncture Research
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• 제18권2호
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• pp.79-90
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• 2001

Objective : The aim of the present study is to investigate whether stimulating a specific auricular point(胃點) is effect on suppression of appetite. Methods : This study evaluated the changes of Reduced-Nicotinamide-Adenine-inucieotide-Phosphate-diaphorase(NADPH-d)-positive neurons using a histoehemical method. Staining intensities of NADPH-d-positive neurons were assessed in a quantitative fashion using a microdensitometrical method based on optical density by means of an image analyzer. Results : The results show as follows ; 1. In the RSG/RSA, FRI/FR2, HL, PAR1 area, The optical density of NADPH-d-positive neurons of fasted group was significantly decreased in comparison to fed group. 2. In most cortical area, It was not significant statistically, But the optical density of NADPH-d-positive neurons of fed with auricular acupuncture group was decreased in comparison to fed group. 3. In most cortical area, It was not significant statistically, But fasted with auricular acupuncture group increased the optical density of NADPH-d-positive neurons comparison to fasted group. Conclusions : We conclude that stimulating the specific auricular points is able to effect on NO(Nitric Oxide)-expression in the brain cortex of sprague dawley rats, The results suggest that auricular acupuncture may be useful for controlling obesity.

• 한국치위생학회지
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• 제4권2호
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• pp.153-163
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• 2004

There are normal inhabitants doing medically useful functions in the body. There are many kinds of bacteria performing specific functions in the oral cavity. Two strains of lactic acid bacteria were isolated from normal inhabitants of children 's oral cavity, which inhibited the the production of halitosis by anaerobic bacteria. The authors identified the isolates by the lest using API 50 CHL medium kit. 1. Two isolates were Gram-positive bacilli and produced hydrogen peroxide. 2. The optical density was 1.286 in the supernatant of Fusobacterium nucleatum after vortexing for 30 minutes, whereas in the supernatant of combined Fusobacterium nucleatum and each isolate, they were reduced to 0.628 and 0.497, which the percentages of coaggregation between them were 29.4% and 57.8%, respectively. 3. The optical density of Fusobacterium nucleatum precipitate was 1.794 in the culture media containing cysteine and $FeSO_4$, being reduced to 1.144 and 0.915 in the coaggregated precipitates of Fusobacterium nucleatum and each isolate. 4. The optical density of Porphyromonas gingivalis precipitate was 1.932 in the culture media, being reduced to 1.170 and 1.266 in the coaggregated precipitates of Porphyromonas gingivalis and each isolate. 5. When two isolates were tested with API 50 CHL medium kit, those were identified as Lactobaciallius salivarius and Lactobacillus delbrueckii subsp. lactis.