Electronic Records need to be appraised the authenticity as well as the value itself. There has been various kinds of discussion about how records to be appraised the value of themselves, but there's little argument about how electronic records to be appraised the authenticity of themselves. Therefore this article is modeling some specific authenticity appraisal methods and showing each stages those methods should or may be applied. At the Ingest stage, integrity verification right after records creation in the organization which produced the records, quality and integrity verification about the transferred in the organization which received the records and integrity check between SIP and AIP in the organization which received and preserved the records are essential. At the Preservation stage, integrity check between same AIPs stored in different medium separately and validation of records where or not damaged and recovery damaged records are needed. At the various Processing stages, suitability evaluation after changing the record's management control meta data and changing the record's classification, integrity check after records migration and periodical validation and integrity verification about DIPs are required. For those activities, the appraisal methods including integrity verification, content consistency check, suitability evaluation about record's meta data, feasibility check of unauthorized update and physical status validation should be applied to the electronic records management process.
Recently, Southeast Asian people, its food, natural sceneries and so on have been increasingly exposed to Korean people through mass media and multi-cultural events. At the same time, Koreans can frequently encounter Southeast Asians in their everyday lives. Thus, specific images and discourses of Southeast Asia has been established in our society, which creates a new social trend called 'Southeast Asia phenomena'. In short, 'Southeast Asia phenomena' means a totality of Korean people's experience of Southeast Asian and their perception on the region. On the one hand, 'Southeast Asia phenomena' is a result of inflow of Southeast Asians and their culture into Korea. On the other hand, it is also a consequence of Korean people's understanding of Southeast Asia from their trip to Southeast Asia or from their interactions with Southeast Asian people. This article aims to analyze the origin and diffusion of 'Southeast Asian Phenomena' in Korea in the context of Southeast Asia focusing on 4 topics, that is, migrant workers, overseas investments, retirement migration, study-abroad categorized as human movement. This article is also about a country-by-country comparative analysis both at the macro level and the micro level. At the macro level, overseas investments and trade, human exchanges, positive perception to Koreans which considered to be the structural causes become a strong mechanism playing a important bridge role between Korea and Southeast Asia. So these create the high probability of the emergence of 'Southeast Asian Phenomena' At the micro level which is more direct causes of 'Southeast Asian Phenomena', the economic cause is the most important common cause for 4 Southeast Asian Phenomena. Additionally, Korean wave is also remarkable common cause creating 'Southeast Asian Phenomena' even it is not the origin in the context of Southeast Asia. The diffusion of 'Southeast Asian Phenomena' is different by the topics and the elements contributing to create the favorable situation for the diffusion are not only overseas investments and trade, human exchanges at the macro level but also policy elements at the micro level. The relative differences of the causes of 'Southeast Asian Phenomena' in the country-by-country analysis are found. Regarding overseas investments in Vietnam and Cambodia, the economic degree of freedom in Cambodia is higher than in Vietnam. Even Korean Wave has had the longer history in Vietnam, but the favorable perspectives on Korean Wave are stronger in Cambodia. For migrant workers from Vietnam and Indonesia, the economic causes in Vietnam are more significant than in Indonesia. The impact of Korean Wave is stronger in Vietnam than in Indonesia. In case of study-abroad, the social-cultural elements and policy elements are more diverse in Malaysia than in Korea. For the Korean retirees who immigrate to the Philippines and Malaysia, the economic causes in the Philippines is more significant in Malaysia.
The cryosuction (negative pore pressure) in freezing soils causes groundwater migration from the frozen fringe to freezing front for ice lens formation. Frost heave and heaving pressure by ice lens cause damage to ground infrastructure. In order to prevent damage by the frost heave, various frost susceptibility criteria have been proposed. The SP (Segregation Potential) is the most widely used classification criterion for frost susceptibility in cold regions. The expansion of the ice lens by the migration of the groundwater is a key role in frost heave mechanism, and thus it is necessary to evaluate the hydraulic conductivity. In this paper, soil mixtures of coarse-fines (sand-silt) were prepared in various weight fractions and used for frost heave and column permeability test. For each case, the SP and the hydraulic conductivity were derived and correlations were analyzed. As a results, the transition threshold of the SP and the hydraulic conductivity were shown at 20% and 50% of the silt weight fraction, respectively. Although there are difference between these transition thresholds, these two coefficients show a specific correlation. In the future, additional study should be conducted for detailed analysis of the threshold transition values between SP and hydraulic conductivity.
Advanced or metastatic breast cancer affects multiple organs and is a leading cause of cancer-related death. Cancer metastasis is associated with epithelial-mesenchymal metastasis (EMT). However, the specific signals that induce and regulate EMT in carcinoma cells remain unclear. PRR16/Largen is a cell size regulator that is independent of mTOR and Hippo signalling pathways. However, little is known about the role PRR16 plays in the EMT process. We found that the expression of PRR16 was increased in mesenchymal breast cancer cell lines. PRR16 overexpression induced EMT in MCF7 breast cancer cells and enhances migration and invasion. To determine how PRR16 induces EMT, the binding proteins for PRR16 were screened, revealing that PRR16 binds to Abl interactor 2 (ABI2). We then investigated whether ABI2 is involved in EMT. Gene silencing of ABI2 induces EMT, leading to enhanced migration and invasion. ABI2 is a gene that codes for a protein that interacts with ABL proto-oncogene 1 (ABL1) kinase. Therefore, we investigated whether the change in ABI2 expression affected the activation of ABL1 kinase. The knockdown of ABI2 and PRR16 overexpression increased the phosphorylation of Y412 in ABL1 kinase. Our results suggest that PRR16 may be involved in EMT by binding to ABI2 and interfering with its inhibition of ABL1 kinase. This indicates that ABL1 kinase inhibitors may be potential therapeutic agents for the treatment of PRR16-related breast cancer.
Purpose - In recent times, the international trade environment has been changing rapidly, centering on the online market. In the post-COVID-19 era, small and medium-sized trading companies are facing the problem of not being properly provided with overseas market research, market trend analysis, and trade-related information. Cloud-based digital trade is being sought as an alternative to solve these problems; however, there is a lack of research on the intention to switch to digital trade among small and medium-sized trading companies. Therefore, this study empirically analyzes the intention to switch to digital trade based on the migration theory, and through this, attempts to identify each factor that affects the intention to switch to digital trade. Design/methodology - In this study, in order to identify factors influencing intention to switch to digital trade and innovation resistance of small and medium-sized trading companies, through previous research on migration theory and the PPM (Push, Pull, Mooring) model, each variable was selected for the purpose of the study. Based on this, a research model was established for the factors affecting switching to digital trade of small and medium-sized trading companies and empirically analyzed. In addition, considering the differences in the innovation propensity and maturity of information infrastructure of trading companies as the recipients of innovation, this study analyzes the moderating effect of the mooring effect and seeks ways to establish specific strategies according to the degree. Findings - As a result of empirical analysis, the pull effect was found to have the greatest influence on intention to switch to digital trade. However, the pull factor was found to have an effect on user resistance, and it was confirmed that it was a factor simultaneously inducing positive and negative consumption behaviors among users. In addition, it was found that the higher the company's innovation propensity, the higher the pull effect's influence on the intention to switch, and analysis showed that the push effect had no influence. In addition, companies with high information infrastructure maturity were expected to have a relatively high level of intention to switch compared to companies with low information infrastructure maturity, and the difference between the two groups was found not to be statistically significant. Originality/value - This study is a timely study in that it demonstrated the effect on the switching to cloud-based digital trade for small and medium-sized trading companies and that the cloud system related to digital trade is in full swing. There are academic implications in that it revealed that the pull effect is an important factor in the intention to switch to cloud service. Practical implications were presented in that small and medium-sized trading companies suggested ways to increase the value of the cloud system for switching to digital trade and a way to increase the switching ratio by minimizing the mooring effect. In addition, the study argues that active institutional support from the government is needed to activate cloud service.
Kim, Da Yeon;Jung, Seok Yun;Kim, Yeon Ju;Kang, Songhwa;Park, Ji Hye;Ji, Seung Taek;Jang, Woong Bi;Lamichane, Shreekrishna;Lamichane, Babita Dahal;Chae, Young Chan;Lee, Dongjun;Chung, Joo Seop;Kwon, Sang-Mo
The Korean Journal of Physiology and Pharmacology
/
v.22
no.2
/
pp.203-213
/
2018
Tumor undergo uncontrolled, excessive proliferation leads to hypoxic microenvironment. To fulfill their demand for nutrient, and oxygen, tumor angiogenesis is required. Endothelial progenitor cells (EPCs) have been known to the main source of angiogenesis because of their potential to differentiation into endothelial cells. Therefore, understanding the mechanism of EPC-mediated angiogenesis in hypoxia is critical for development of cancer therapy. Recently, mitochondrial dynamics has emerged as a critical mechanism for cellular function and differentiation under hypoxic conditions. However, the role of mitochondrial dynamics in hypoxia-induced angiogenesis remains to be elucidated. In this study, we demonstrated that hypoxia-induced mitochondrial fission accelerates EPCs bioactivities. We first investigated the effect of hypoxia on EPC-mediated angiogenesis. Cell migration, invasion, and tube formation was significantly increased under hypoxic conditions; expression of EPC surface markers was unchanged. And mitochondrial fission was induced by hypoxia time-dependent manner. We found that hypoxia-induced mitochondrial fission was triggered by dynamin-related protein Drp1, specifically, phosphorylated DRP1 at Ser637, a suppression marker for mitochondrial fission, was impaired in hypoxia time-dependent manner. To confirm the role of DRP1 in EPC-mediated angiogenesis, we analyzed cell bioactivities using Mdivi-1, a selective DRP1 inhibitor, and DRP1 siRNA. DRP1 silencing or Mdivi-1 treatment dramatically reduced cell migration, invasion, and tube formation in EPCs, but the expression of EPC surface markers was unchanged. In conclusion, we uncovered a novel role of mitochondrial fission in hypoxia-induced angiogenesis. Therefore, we suggest that specific modulation of DRP1-mediated mitochondrial dynamics may be a potential therapeutic strategy in EPC-mediated tumor angiogenesis.
The Journal of the Korean Society for Microbiology
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v.21
no.1
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pp.133-144
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1986
This study was undertaken to assess the effect of ginseng administration on T lymphocyte induced local xenogenic graft-versus-host(GVM) reactions which were induced with thymocyte, spleen cell and lymph node cell of ICR mice. Mice received daily 10mg of 70% alcohol ginseng extract oral1y for 100days and control mice remained untreated for the same period of time. The cells from donor mice were injected intradermally into the closely shaven abdominal skin of Sprague-Dawley rats for GVH tests. The thymocyte from control(ginseng-untreated) mice showed a negative local GVH reaction, whereas thymocyte from experimental(ginseng-treated) mice showed a positive reaction with the rate of 17.4%. When spleen cells were injected, the incidence of positive local GVH reaction was 66.7% among ginseng-treated mice, as opposed to incidence of 45.5% of positive local GVH reaction among control mice. The incidence of positive local GVH reaction of the lymph node cells when injected into a recipient was 71.4% among ginseng-treated mice as compared with that of 18.9% among control mice. The relationship between spleen cell inoculum and intensity of the local GVH reaction was assessed in ginseng-untreated mice. The intensity of GVH reaction clearly appears to be dose related. In ginseng-treated mice, a minimum of $1{\times}10^7$ spleen cell was required for production of positive local GVH reaction with almost linear relationship up to an inoculum of $5{\times}10^8$ cells. In control mice, however, a minimum of $1{\times}10^8$ spleen cells was required for positive GVH reaction. These results strongly suggest that the ginseng administration augments significantly the local xenogenic GVH reaction which was used to assess T lymphocyte function and immunocompetence of mice and in addition to this, these results appear to support previous suggestions that the local GVH reaction consitutes a qualitative test of the functional activity of T lymphocytes. These results may be the first to induce local GVH reaction, employing rats as recipient and mice as donor. This study was also desingned to investigate some of the effects of ginseng extract on lymphocyte-macrophage interactions. This was accomplished by in vitro quantification of 1) migratory inhibitory factor(MIF) synthetic capacity of splenic lymphocytes in mice previously primed with ginseng 2) MIF responsiveness of mouse peritoneal macrophages or chicken peripheral leucocytes under the presence of ginseng extract 3) migration ability of chicken peripheral leucocytes by direct stimulation of ginseng extract or ginseng saponin and 4) immunosuppressive effects of immunosuppressants such as cyclophosphamide, cyclosporin A or dexamethasone. Mice divided equally into the ginseng and the saline groups, which received intraperitoneally daily 0.2ml of ginseng absolute alcohol-extract(5mg/ml) and same amount of saline for 15 days, respectively. The cellular immune responsiveness of these mice was assayed 15 days after ginseng pretreatment. Splenic lymphocytes of mice treated with ginseng, when stimulated with sensitized specific-antigen such as sheep red blood cells or toxoplasmin, or with polyclonal activator concanavalin A, produced significantly more MIF than those of control saline group. MIF responsiveness of normal mouse macrophages was significantly augmented when assayed under the presence of ginseng extract (1mg/ml). The migratory ability of normal chicken leucocytes in the absence of MIF was significantly decreased by the stimulation of ginseng extract alone. MIF response was significantly decreased by immunosuppressants and this impaired response was not restored by ginseng pretreatment. This study was additionally performed to evaluate the effect of ginseng on the expulsion of adult Trichinella spiralis in mice. ICR mice were infected experimentally by esophageal incubation of 300 T. spiralis infective muscle larvae prepared by acid-pepsin digestion of infected mice. and received oral administration of 70% alcohol ginseng extract(10mg/mouse/day) for the indicated days plus 4 days before infection. At various times after infection, the number of adult T. spiralis worms in small intestines was determined. Interestingly, ginseng-treatment was accompanied by accelerated expulson of T. spiralis. These results led to the conclusion that Panax ginseng caused some enhancing effect on GVH reaction, macrophage migration inhibition reaction and expulsion of T. spiralis. In addition these results suggested that the mechanisms responsible for this enhancement of ginseng may be chiefly or partially due to nonspecific stimulation of cell-mediated immune response.
Placenta-derived mesenchymal stem cells (PD-MSCs) are promising candidates for cell-based therapy in regenerative medicine. The migration and homing potential of PD-MSCs to injured sites is a critical property of MSC engraftment. MicroRNAs (miRNAs) have recently been shown to regulate the critical functions of MSCs, such as proliferation, survival, and migration. The objective of the present study was to identify the miRNA and target genes involved in PD-MSCs homing in a bile duct ligation (BDL) rat model. We selected candidate miRNAs targeting genes for PD-MSCs homing based on microarray analysis. PD-MSC engraftment in BDL-injured rat liver was identified by immunofluorescence assay and human-specific Alu gene expression by quantitative real-time polymerase chain reaction (qRT-PCR) one week after transplantation. Compared with migrated naïve PD-MSCs under hypoxic and normoxic conditions (Hyp/Nor), the transplanted group with PD-MSCs (Tx) showed distinct differences in miRNA expressions in BDL-injured rat liver. We also validated the miRNAs and their target genes for PD-MSCs homing. The expressions of integrin α4 (ITGA4) and integrin α5 (ITGA5) target genes for miR-199a-5p and miR-148a-3p were significantly upregulated in the Tx group (p<0.05). In addition, integrin β1 (ITGB1) and integrin β8 (ITGB8) were upregulated by suppressing miR-183-5p and miR-145-5p, respectively. These results demonstrated that PD-MSCs regulate miRNA expression related to the integrin family for their homing effects on the BDL-injured rat liver. The findings further suggest that miRNA-mediated regulation of the integrin family contributes to the therapeutic efficacy of PD-MSCs in the rat hepatic fibrosis model by BDL.
Literature has been special for the Polish who suffered from the numerous invasions from surrounding countries for her geographical location at the center of Europe. In the late 18th century at a time when Poland was divided and ruled by Russia, Prussia and Austria, literature played an important role in uniting Poland. During the 2nd world war in which Poland was occupied by the Soviet Union and by Germany, and during the Cold War period under socialism system(1948~1989), the Polish literature was in the front to keep unique national culture with overseas migration community at the center. The Polish Diaspora literature from 19th century up to now has naturally embodied national sufferings from foreign powers in their literary tradition linked to the problem of 'migration'. In addition, they belong to other cultural sphere, but keep their own unique identity, which is similar to Korean Diaspora literature to a great degree. This study has two stages. In the first stage, it figures out the formation and trend of the Polish Diaspora literature followed by their meaning in the history of Polish literature. In the second stage, specific texts (two dramas) are analyzed before and after system transition in 1989. * Before system transition: S. Mrożek, Emigranci (1974), * After system transition: J. Głowacki, Antygona w Nowym Yorku (1992) Mrożek and Głowacki had themselves migration experiences with high achievement and recognition in literature not only in Poland but also in the world. In their works, hardships as 'strangers' in foreign countries, emotional wandering and agony, nostalgia to lost home land and exploration of identity were described vividly. By comparing the 2 literature texts, this study attempts to trace the change of Diaspora consciousness which Polish migrants experienced in foreign countries with different political system like socialism and capitalism.
Seasonality in abundance and egg production was investigated for Calanus sinicus in Asan Bay, Korea, and feeding effort was measured in the laboratory condition. Although abundances of this species in this bay showed only one Peak in spring, egg Production showed two peaks, spring and fall, in this bay. Potential of year-round egg Production was also found. Food availability judged by the particulate organic carbon (POC) concentration was usually more than enough in this bay. High egg Production in fall resulted in spring Peak in abundance. But high egg Production in spring did not result in summer or fall Peak in abundance. Low abundances in summer and fall were believed to be the result of long range horizontal migration out to the central Part of the Yellow Sea. The highest average egg Production by the Population of this species was 16.3 eggs female-'day-'. Maximum e99 Production by an individual was 39.0 e99s female$^{-1}$ day$^{-1}$, which yielded 30.7% of specific egg Production rate in terms of body carbon content with the carbon contents of 44.44$^{\mu}$gC and 0.35 $^{\mu}$gC for individual adult female and egg, respectively. Clearance rate (F) measured in the laboratory condition decreased exponentially with the increase of food concentrations, as expected. The asymptote of F was 3.17 ml copepod$^{-1}$ h$^{-1}$. When this value and the minimum concentration of POC measured in the field were applied under the assumption of continuous feeding in the nature, the calculated gross efficient of egg production was 41.6% in case of average egg production of the population. Violation of the assumption of diurnal feeding rhythm and application of the minimum concentration of POC may offset the influence on calculation each other.
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