• Korean Journal of Agricultural Science
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• v.48 no.4
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• pp.1003-1013
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• 2021

Thioredoxin (TRX) protein is an antioxidant responsible for reducing other proteins by exchanging cysteine thiol-disulfide and is also known for its anti-allergic and anti-aging properties. On the other hand, epidermal growth factor (EGF) is an important material used in the cosmetics industry and an essential protein necessary for dermal wound healing facilitated by the proliferation and migration of keratinocytes. EGF also assists in the formation of granulation tissues and stimulates the motility of fibroblasts. Hence, genetically modified soybeans were developed to overexpress these industrially important proteins for mass production. A single-dose oral-toxicity-based study was conducted to evaluate the potential toxic effects of TRX and EGF proteins, as safety assessments are necessary for the commercial use of seed-specific protein-expressing transgenic soybeans. To achieve this rationale, TRX and EGF proteins were mass purified from recombinant E. coli. The single-dose oral-toxicity tests of the TRX and EGF proteins were carried out in six-week old male and female Institute of Cancer Research (ICR) mice. The initial evaluation of the single-dose TRF and EGF treatments was based on monitoring the toxicity signatures and mortality rates among the mice, and the resultant mortality rates did not show any specific clinical symptoms related to the proteins. Furthermore, no significant differences were observed in the weights between the treatment and control groups of male and female ICR mice. After 14 days of treatment, no differences were observed in the autopsy reports between the various treatment and control groups. These results suggest that the minimum lethal dose of TRX and EGF proteins is higher than the allowed 2,000 mg·kg^-1 limit.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.4
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• pp.2571-2575
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• 2013

AKT1 is a member of the serine/threoine AGC protein kinase family involved in thyroid cancer metabolism, growth, proliferation and survival. It is overexpressed in thyroid tumors. In this study, we designed two AKT1 specific DNAzymes (DRz1 and DRz2) that target AKT1 mRNA. The results showed that DRz1 could decrease the expression of AKT1 by 58%. Furthermore, DRz1 significantly inhibited cell proliferation, induced apoptosis and inhibited invasion in SW597 cells. In addition, down-regulation of survivin expression was associated with decreased caspase-3, VEGF and MMP2 in SW597 cells after 24 h. In our study, the efficacy of DRz1 in decreasing AKT1 protein levels were better than DRz2. AKT1-DRz1 might have anti-tumorigenic activity and may provide the basis for a novel therapeutic intervention in thyroid cancer treatment.

• 한국신재생에너지학회:학술대회논문집
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• 2010.06a
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• pp.163.2-163.2
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• 2010

In this work, nano-flake shaped nickel oxide (NiO) films were synthesized by chemical bath deposition technique for electrochemical capacitors. The deposition was carried out for 1 and 2 h at room temperature using nickel foam as the substrate and the current collector. The structure and morphology of prepared NiO film were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). And, electrochemical properties were characterized by cyclic voltammetry, galvanostatic charge-discharge, and AC impedence measurement. It was found that the NiO film was constructed by many interconnected NiO nano-flakes which arranged vertically to the substrate, forming a net-like structure with large pores. The open macropores may facilitate the electrolyte penetration and ion migration, resulted in the utilization of nickel oxide due to the increased surface area for electrochemical reactions. Furthermore, it was found that the deposition onto nickel foam as substrate and curent collector led to decrease of the ion transfer resistance so that its specific capacitance of a NiO film had high value than NiO nano flake powder.

• 한국석유지질학회:학술대회논문집
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• spring
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• pp.22-28
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• 1998

Twenty species of calcareous nannofossils belonging to 11 genera are identified from the Seoguipo Formation in Cheju island, Korea. On the basis of the marker species, the Seoguipo Formation is biostratigraphically assigned to the Pseudoemiliania lacunosa Zone (NNl9), which corresponds to the combined zones of Emiliania annula - Emiliania ovata (CN13a-CN14a) of latest Pliocene and Early Pleistocene. Generally, cold-water species is dominant in the lower part, whereas warm-water one in the upper part. This is interpreted the palaeoceanographic condition has changed from cooling to warm phase. The change in floral composition and abundance of specific species allows the recognition of 4 ecostaratigraphic units in the Seoguipo Formation and the migration of oceanographic frontal boundary. According to nannofossil distribution in the study area, the position of an oceanographic boundary between warmer water and cooler water appeared to have oscillated north-south over the Korea Strait and Cheju island in response to glacial and interglacial cycles. The geologic time of the interpreted paleoceanographical changes determined by nannofossil biochronology is well agreed with the results obtained from the Japan Sea (East Sea) and Japan-Sea side of Japan.

• Textile Coloration and Finishing
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• v.28 no.4
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• pp.280-289
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• 2016

Silica microgel was prepared by sol-gel process and then functional groups, epoxy group and ethoxy group, were introduced on the particle by coupling treatment with 3-glysidoxypropyltrimethoxysilane. The functional silica microgel(functional SiGel) formation was identified using FT-IR spectrometry. Phase stability for DMF solution of functional SiGel, PU resin and pigment was evaluated by Turbiscan Lab. And physical properties of artificial suede including hand values, morphology and dyeing fastness were investigated. The mean particle size and the specific surface area of the functional SiGel were $0.77{\mu}m$ and $380m^2/g$. Mixture containing the functional SiGel, PU resin and pigment was more stable than the functional SiGel-free mixed solution. Artificial suede prepared by the functional SiGel had appearance and feeling close to natural suede. The migration fastness, the solvent wicking fastness and rubbing fastness of the artificial suede were enhanced to 4~5 grades, 4~5 grades and 3~4 grades, respectively.

• Journal of Technology Innovation
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• v.13 no.2
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• pp.179-205
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• 2005

This study reveals that the user industry has a limited role in being a source of technological capability in the case of the machine tool industry in Korea where the user industry is relatively more advanced than other capital goods industries. This study examines the sources of technological capability in terms of migration of workforces and flow of product development knowledge. Although the capital goods sector is generally regarded as being the sector where user-producer interaction is important, the user industry is not the seed-bed of technological capability for machine development. Users and producers interact in terms of expressing 'needs', mainly in the form of specifications. As a result of receiving unique specifications from users, the producer learns to react by making specific customised special purpose machines. The user's specification could include information o the imported machine originally used. When confronted with technical problems in developing a new machine, the producer accesses foreign sources of knowledge. This study's finding reveals that users of special purpose machines have a significantly clearer role in providing specifications than do users of general purpose machine tools. Most intensive interactive learning between users and producers in the production process is found in special purpose machine tools. From the empirical findings, policy implications are discussed.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.2
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• pp.831-836
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• 2015

Tumor fluid accumulation occurs in both human cancer and experimental tumor models. Solid tumors show a tendency to tumor fluid accumulation because of their anatomical and physiological features and this may be influenced by molecular factors. Fluid accumulation in the peri-tumor area also occurs in the Dunning model of rat prostate cancer as the tumor grows. In this study, the effects of tumor fluids that were obtained from Dunning prostate tumor-bearing Copenhagen rats on the strongly metastatic MAT-LyLu cell line were investigatedby examining the cell's migration and tumor fluid's toxicity and the kinetic parameters such as cell proliferation, mitotic index, and labelling index. In this research, tumor fluids were obtained from rats injected with $2{\times}10^5$ MAT-LyLu cells and treated with saline solution, and 200 nM tetrodotoxin (TTX), highly specific sodium channel blocker was used. Sterilized tumor fluids were added to medium of MAT-LyLu cells with the proportion of 20% in vitro. Consequently, it was demonstrated that Dunning rat prostate tumor fluid significantly inhibited proliferation (up to 50%), mitotic index, and labeling index of MAT-LyLu cells (up to 75%) (p<0.05) but stimulated the motility of the cells in vitro.

• Korean Journal of Oriental Medicine
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• v.14 no.3
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• pp.1-10
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• 2008

This paper treats with transitional development of medico-hygienical situation in district Yanbian along with the evolution in specific field of medicine. This work is particularly important in shaping TKM identity as TCM embraces Chao medicine asserting it as one included in TCM. This research deals with themes of migration of Chao minorities to this territory and their medico-hygienical situation. Lifted bans on immigration in late Qing dynasty with uncertainty of Korea(Chosun) triggered the immigration to this district. The flow was heavily strengthened under the influence of Westerners and Japanese imperialism into china which consequently provoked the ruin of Qing dynasty, the civil war between republicans and communist and the socio-political changes in Korea. As population growths, the establishment of hospitals and immigration of healthcare professionals were also increased. Though this district is located in Chinese mainland the influence of Japanese was also relevant which lead to medical practice reflection both sides. Mutual combination and influence of western(contemporary) medicine, TCM and TKM practices made the particularity of chao medicine.

• Journal of Environmental Science International
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• v.20 no.4
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• pp.551-554
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• 2011

The production of the sharp-toothed eel by commercial catch off waters of Korea is annually declined after 1978. This study was carried out to obtain the stock management of the sharp-toothed eel using the PCR-aided RFLP method. The mtDNA COI gene was amplified using species-specific primers and PCR product was observed to 700 bp. Amplified DNA fragments were treated with six kinds of restriction enzymes (BaeHI, EcoRI, PstI, Ksp22, HinfI and HaeIII). The treatment of HaeIII showed a distinct PCR product between Yeosu/Jinhae/Jeju/Goseoung and Jangheung/Haenam populations that were observed from 300 to 400 bp in reference to 100 bp molecular marker. However, DNA fragment within populations had an identical pattern. The phylogenetic homology is 82% between two populations inferred from RFLP PCR product pattern using NTsysPC ver. 2.1. The use of HaeIII plays an important role in discriminating populations. It is thought that adults after over-wintering in the southern part of Jeju migrate to the Yeosu, Jinhae and Goseoung regions to spawn instead of to southwestern waters. Individuals within populations showed a relatively active genetic mixing and migration regardless of geography. However, the genetic ancestor of Jangheung and Haenam populations is appeared to be more adjacent to China or Japan than Jeju.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.3
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• pp.927-932
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• 2016

Bone morphogenetic proteins (BMPs), belonging to the transforming growth factor-${\beta}$ superfamily, regulate many cellular activities including cell migration, differentiation, adhesion, proliferation and apoptosis. Use of recombinant human bone morphogenic protein-2 (rhBMP-2) in oral and maxillofacial surgery has seen a tremendous increase. Due to its role in many cellular pathways, the influence of this protein on carcinogenesis in different organs has been intensively studied over the past decade. BMPs also have been detected to have a role in the development and progression of many tumors, particularly disease-specific bone metastasis. In oral squamous cell carcinoma - the tumor type accounting for more than 90% of head and neck malignancies- aberrations of both BMP expression and associated signaling pathways have a certain relation with the development and progression of the disease by regulating a range of biological functions in the altered cells. In the current review, we discuss the influence of BMPs -especially rhBMP-2- in the development and progression of oral squamous cell carcinoma.