• Title/Summary/Keyword: Specific Growth Rate

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Effect of Growth Rate and Cultivation Temperature on the Yeast RNA Accumulation and Autolysis Efficiency (생육속도 및 배양온도가 효모 RNA 축적과 autolysis 효율에 미치는 영향)

  • Kim, Sung-Yong;Kwon, Oh-Sung;Nam, Hee-Sop;Lee, Hyung-Jae
    • Korean Journal of Food Science and Technology
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    • v.27 no.1
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    • pp.129-133
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    • 1995
  • Continuous fermentations were performed in order to investigate the effect of culture condition on the yeast RNA accumulation and autolysis efficiency. The content of intracellular RNA increased with increasing dilution rate, showing its maximum value of 14.8% at D=0.35 $h^{-1}$. Also, both RNA productivity and specific RNA productivity tended to increase with the increase of dilution rate. The maximum biomass was obtained at $30^{\circ}C$ in the fixed dilution rate of 0.2 $h^{-1}$, whereas the maximum RNA content appeared at the lowest temperature experimented. Growth rate affected significantly on the yeast autolysis efficiency such that the extraction ratio(TN/TN) increased with increasing growth rate, whereas the hydrolysis ratio(AN/TN) was reversed. On the other hand, its efficiency was little affected by cultivation temperature.

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연속배양을 통한 L-prolinc 발효공정의 최적화 연구

  • Yu, Ji-Myeong;Choe, Sun-Yong;Jang, Hyeong-Uk;An, Jeong-O;Jo, Yeong-Il;Lee, Hong-Won;Jeong, Jun-Gi
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.426-429
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    • 2001
  • The continuous production of L -proline was studied using L-histidine auxotrophic mutant of Corynebacterium acetoacidophilum under various substrate limited conditions. Among the $NH_4\;^+$ $PO_4\;^3$ and L -histidine limited conditions, the highest production of L -proline was observed under the L-histidine limited condition. Under $NH_4\;^+$ and $PO_4\;^3$ limited conditions, no or poor L-proline production was observed, respectively. For the kinetic parameters under L -histidine limitation the specific rate of L -proline production was increased with dilution rate upto $0.1hr^{-1}$ but decreased above $0.1hr^{-1}$. The volumetric rate of L -proline production was showed similar pattern with specific rate. The dried cell weight was gradually increased according to decrease the dilution rate. Specific rate of glucose consumption was proportionally increased with dilution rate. The results of continuous culture (higher production of L-proline at dilution rate $0.1hr^{-1}$) will be used in fed-batch culture for the control of cell growth rate and mass production of L-proline.

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Effects of Nitrogen and Sodium on Growth in Phaeodactylum tricornutum (Bacillariophyceae)

  • Lee Soon Jeong;Choi Han Gil;Nam Ki Wan
    • Fisheries and Aquatic Sciences
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    • v.3 no.2
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    • pp.151-155
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    • 2000
  • Phaeodactylum tricornutum (Bacillariophyceae) is a marine diatom which has been supplied as a food of bivalves. In this study, growth responses of P. tricornutum to some nitrogen sources and sodium were investigated by measuring cell number and contents of chlorophyll a in culture. In medium with nitrogen and sodium, brisk cell division occurred and maximum growth rate was respectively found in the medium with 150 mg/l of nitrate and 10 mg/l of ammonium and urea. At 10-500 mg/l ammonium and urea and 200-500 mg/l nitrate, specific growth rate decreased slightly. However, no cell division observed in sodium-deficient medium, regardless of presence or absence of nitrogen. This suggests that sodium is required for the nitrogen uptake of P. tricornutum, resulting nitrogen uptake leading to cell division. Also the upper limits of ammonium and nitrate for the growth of P. tricornutum seem to be 10 mg/l and 500 mg/l, respectively.

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Studies on Wood Quality and Growth of Quercus rubra (24 Years Old) in Korea - Physical and Mechanical Properties - (24년생 루브라참나무의 생장과 재질에 관한 연구 - 물리·역학적 성질 -)

  • Han, Mu-Seok;Lee, Chang-Jun;Park, Bong-Seok;Kim, Byung-Ro
    • Journal of the Korean Wood Science and Technology
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    • v.42 no.3
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    • pp.327-338
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    • 2014
  • Relationship between growth rate and wood quality was investigated by physical and mechanical properties with Quercus rubra (24 years old) from five different origin of apricot. In greenwood moisture content, sapwood had higher moisture content than heartwood, and there was difference among different origin of apricot. There were different specific gravity of wood among different origin of apricot. Compared with higher growth rate with higher specific gravity in sapwood, opposite trend was observed in heartwood. There were difference in shrinkage based on origin of apricot, and higher growth rate wood had higher shrinkage and T/R ratio. Compression Young's modulus, bending strength, bending Young's modulus, and compact strength was difference among different origin of apricot. Higher growth rate wood had higher tensile strength, and also there was difference amont different origin of apricot. In hardness, 3 different directions had all difference among different origin of apricot, and higher growth rate wood showed higher hardness than others. Based on overall physical and/or mechanical properties and growth rate, apricot from Bancroft was best quality in current.

Aquatic Plants for Wastewater Treatment (수생식물을 이용한 수질정화에 관한 연구)

  • 나규환;권성환;이장훈
    • Journal of Environmental Health Sciences
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    • v.22 no.3
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    • pp.49-55
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    • 1996
  • Water parsley(Oenanthe javanica(Blume) DC) was raised with varying population density(S) in the laboratory aquarium unit to determine the growth equation. The population density was measure after 7 days. The resultant growth curve was well fit to the equation 1/S = A+B (1/S0) with a high correlation coefficient ($R^2$ = 0.999). The maximum specific absorption rate was $9.011 \times 10^{-5}$ kg $NO_x-N/kg$ water parsley$\cdot$day and $1.31 \times 10^{-5}$ kg $PO_4-P/kg$ water parsley$\cdot$day when the average population density was $2.62 kg/m^2$. The relationship between population density and nutrient absorption rate, the absorption rate of $NO_x-N$ was 5.04~5.24 mg/l$\cdot$day when the population density was $7.51~10.0 $mg/m^2\cdot day$ and the absorption rate of $PO_4-P$ was 0.56~0.78 mg/l$\cdot$day when the population density was 5.02~10.0 $kg/m^2\cdot day$. Taking into account the nutrient absorption rate and growth rate, the population density between $7.0 kg/m^2\cdot day$ and $8.0 kg/m^2 \cdot day$ was selected. The removal rate of nutrient was investigated after 7 days culture. Removal rate of $NO_x-N$ was 95.6~99.95% with initial concentration of 35 mg $NO_x-N/l$, and the removal rate of $PO_4-P$ was also high, indicating 80.24~98.9% with initial concentration of 5.95 mg $PO_x-P/l$.

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Effects of Substrate Size on the Growth of 4 Microphytobenthos Species (Achnanthes sp., Amphora sp., Navicula sp. and Nitzschia sp.) (저서미세조류 4종(Achnanthes sp., Amphora sp., Navicula sp. 그리고 Nitzschia sp.)의 성장에 영향을 미치는 부착기질 크기의 영향)

  • Kwon, Hyeong-Kyu;Yang, Han-Soeb;Yu, Yeoung-Moon;Oh, Seok-Jin
    • Journal of Environmental Science International
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    • v.21 no.1
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    • pp.105-111
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    • 2012
  • The effects of substrate size on the growth of microphytobenthos Achnanthes sp., Amphora sp., Navicula sp. and Nitzschia sp. were examined using glass beads in order for phytoremediation in the benthic layer of coastal waters. The glass beads used in this study were 0.09~0.15 mm (G.B 1), 0.25~0.50 mm (G.B 2), 0.75~1.00 mm (G.B 3) and 1.25~1.65 mm (G.B 4). No addition of glass bead used as control. The specific growth rate and maximum cell density of four microphytobenthos species were increasing with decreasing size of glass beads. Moreover, the control experiment without added attachment substrates showed the lowest specific growth rate and maximum cell density. Therefore, the suitable attachment substrates for mass culture of microphytobenthos seems to be important in order for phytoremediation using microphytobenthos.

The study on the $CO_2$ fixation and algae reproduction by microalgae Chlorella ellipsoidea (Chlorella ellipsoidea를 이용한 $CO_2$ 고정 및 미세조류 증식효과 검토)

  • 강창민;홍순강
    • Journal of environmental and Sanitary engineering
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    • v.15 no.1
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    • pp.39-45
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    • 2000
  • The purpose of this study was conducted to indentified the fixing quality of $CO_2$, the most important greenhouse effect gas, by microalgae Chlorella ellipsoidea in batch test apparatus. The glass flask of $1.4{\ell}$ culture media which was saturated with 99.99% pure $CO_2$ gas was setted water bath of $25^{\circ}C$, 5000Lux, and seeded 100$m\ell$ algae liquid. We checked the change of inorganic carbon concentration and algae population with time in culture media. The result were next: the growth of algae population relied on aquatic IC(inorganic carbon) concentration. And the pH was increased with decrease of IC concentration. The growth of algae population had positive correalation with $CO_2$ concentration, and the coefficient of correlation was 0.982. The specific growth rate($\mu$) of Chlorella ellipsoidea was 1.104/d, the maximum specific growth $rate({\mu}_{max}$) of 9.21/d, and helf velocity constant($K_s$) of $259mg/{\ell}$ by Monod equation.

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Compensatory Responses of Nile Tilapia Oreochromis niloticus under Different Feed-Deprivation Regimes

  • Gao, Yang;Lee, Jeong-Yeol
    • Fisheries and Aquatic Sciences
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    • v.15 no.4
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    • pp.305-311
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    • 2012
  • We investigated compensatory growth of Nile Tilapia Oreochromis niloticus in structural size and live weight in response to different deprivation periods and refeeding. Four treatments were assigned randomly to fish in 12 glass tanks, with each treatment performed in triplicate. The control group was fed to satiation three times a day throughout the experiment. The other three treatment groups were starved for 1 week (S1), 2 weeks (S2), or 4 weeks (S4) and then fed until the end of the experiment. After the experiment, no significant differences were observed among S1, S2, and the control group in average weight or length, whereas the weight and length of S4 were significantly reduced. Relative condition factors of the three starved groups decreased significantly until the end of the restricted period but recovered rapidly after refeeding. The specific growth rate in weight ($SGR_W$) of the three restricted groups recovered quickly upon refeeding and were significantly higher than the control group, but these differences disappeared gradually until the end of the experiment. No significant difference in specific growth rate in length ($SGR_L$) was noted between the control group and the three restricted groups after refeeding. All three groups showed hyperphagia for a short period upon refeeding, and no statistical differences were observed in feeding efficiency among the four groups.

On-off Control for Continuous Culture of Microalgae in Flat Panel Photobioreactor (평판형 광생물반응기의 미세조류 연속배양을 위한 On-off 제어)

  • Kim, Jae-Hyeok;Yoon, Chung-Man;Jeong, Sang-Hwa
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.25 no.3
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    • pp.237-243
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    • 2016
  • Recently, technologies that produce biofuels from microalgae are being studied worldwide. It is necessary to significantly reduce the production costs of biofuels from microalgae for economic reasons. In this study, the growth curve of the microalgae was obtained using the batch-culture method, and the specific growth rate was predicted using the regression method. Based on the culture conditions of the estimated specific growth rate, the turbidity of the microalgae in the flat panel photobioreactor (PBR) was measured. Furthermore, an on-off control scheme was applied to the flat panel PBR in order to culture the microalgae continuously on the basis of turbidity. The parameters of the on-off control system were displayed by LabView. The on-off scheme of peristaltic pump was controlled based on the turbidity in the PBR. In addition, the turbidity values of growth curves were compared and analyzed in the continuous culture process using the on-off controller.

Effects of Photoperiods and Body Size on the Off-season Smolt Production of Atlantic Salmon Salmo salar in a Recirculating Aquaculture System (순환여과양식시스템에서 광주기 및 어체 크기가 대서양연어(Salmo salar)의 Off-season Smolt 생산에 미치는 영향)

  • Kim, Youhee;Kim, Pyong Kih
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.6
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    • pp.894-902
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    • 2022
  • This study investigated the effects of photoperiod (NL 12L:12D and LL 24L:0D) and body sizes (30 g and 50 g) on parr-smolt transformation, post-smolt growth and blood properties in the off-season parr-smolt stage of Atlantic salmon reared in a recirculating aquaculture system (RAS). Potential off-season salmon smolt were reared in a freshwater RAS for 80 days and then all experimental fish were transferred to seawater. In both LL groups (LL-30 and LL-50), we recorded and increase in specific growth rate and reduction in feed conversion, although there were no significant difference in body size. The values of osmolality, and serum Na+, Cl- and cortisol concentrations in the LL groups were maintained at lower levels than in NL group fish, and LL group fish were observed to recover to the pre-seawater adaptation state more rapidly than those in the NL group. ID chips were inserted in all smolts reared in freshwater. These fish were subsequently transferred to full-strength seawater and thereafter individual growth rates were monitored for 120 days. The results indicated that compared with smolt reared under natural photoperiodic condition, 24 h lighting in freshwater contributed to enhancing post-smolt specific growth rate in seawater.