• Microbiology and Biotechnology Letters
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• v.19 no.1
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• pp.21-24
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• 1991

The promoter isolated from an alkali-tolerant Hwillus sp. chromosomal DNA was subcluned. The activity of promoter in B, subtilis and alkali-tolerant Bacillus sp. began to increase at the early stage. of spore formation. In the presence of 1% glucose, the promotcr activity repressed and was recovered by ;tddition of c-GMP in the medium.

• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.913-922
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• 2013

An agar-degrading bacterium was isolated from red seaweed (Gelidium amansii) on a natural seawater agar plate, and identified as Saccharophagus sp. AG21. The ${\beta}$-agarase gene from Saccharophagus sp. AG21 (agy1) was screened by long and accurate (LA)-PCR. The predicted sequence has a 1,908 bp open reading frame encoding 636 amino acids (aa), and includes a glycosyl hydrolase family 16 (GH16) ${\beta}$-agarase module and two carbohydrate binding modules of family 6 (CBM6). The deduced aa sequence showed 93.7% and 84.9% similarity to ${\beta}$-agarase of Saccharophagus degradans and Microbulbifer agarilyticus, respectively. The mature agy1 was cloned and overexpressed as a His-tagged recombinant ${\beta}$-agarase (rAgy1) in Escherichia coli, and had a predicted molecular mass of 69 kDa and an isoelectric point of 4.5. rAgy1 showed optimum activity at $55^{\circ}C$ and pH 7.6, and had a specific activity of 85 U/mg. The rAgy1 activity was enhanced by $FeSO_4$ (40%), KCl (34%), and NaCl (34%), compared with the control. The newly identified rAgy1 is a ${\beta}$-agarase, which acts to degrade agarose to neoagarotetraose (NA4) and neoagarohexaose (NA6) and may be useful for applications in the cosmetics, food, bioethanol, and reagent industries.

• Journal of Environmental Health Sciences
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• v.21 no.4
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• pp.44-48
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• 1995

26 bacterial strains capable of growing on Terephthalic acid (TPA) in minimal medium were isolated from soil and wastewater by selective enrichment culture, and among them, one isolate which was the best in the cell growth and TPA degradation was selected and identified as Pseudomonas sp. T-1 by its characteristics. Cell growth almost revealed a stationary phase at 24 hrs after cultivation. Cell growth dramatically increased in a minimal medium containing 0.1% of TPA as a sole carbon source and TPA was not detected any more at 80 hrs after cultivation. Therefore, it is suggested that Pseudomonas sp. T-1 could be effectively used for the biological treatment of wastewater containing TPA.

• Journal of Life Science
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• v.18 no.12
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• pp.1723-1727
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• 2008

Chlorella sp. CMS-1 strain was isolated from the outdoors cultivation pools in Culmansa Co., Ltd. This strain was found to be a rounded type of 3 ${\mu}m$. Phylogenetic analysis by the 18S rRNA sequencing using isolated strain is most similar to Chlorella sp. IFRPD 1018 gene at the level of nucleotide sequence identity at 99%. Accordingly, the isolated Chlorella strain was named as Chlorella sp. CMS-1 based on its morphological and phylogenetic properties. The concentrations of crude protein and fat were 59% and 0.01%, respectively. Major compositional amino acids (mg%) were glutamic acid 6.21, alanine 5.76, aspartic acid 5.44%, glycine 4.29%, and threonine 3.09% and major free amino acids (mg%) were ${\gamma}$-aminobutyric acid (GABA) 7.13%, L-alanine 1.44%, L-glutamic acid 0.90, L-leucine 0.26% and L-glycine 0.20%. The concentrations of major minerals were P 2.25%, K 2.25%, Na 1.09%, Mg 0.63%, and Ca 0.28%.

• Natural Product Sciences
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• v.21 no.4
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• pp.248-250
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• 2015

A new ${\alpha}-pyrone$ derivative, violapyrone J (1), and along with the two known violapyrones B (2) and C (3) were isolated from the fermentation broth of a marine actinomycete Streptomyces sp. SC0718. The structure of violapyrone J (1) was elucidated from 1D and 2D NMR spectroscopic analyses.

• Journal of Veterinary Clinics
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• v.19 no.3
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• pp.283-289
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• 2002

The effects of electroacupuncture (EA) at SP-6 with different durations on the minimum alveolar concentration (MAC) and on the cardiovascular system were evaluated in dogs under isoflurane anesthesia. Eight healthy male beagles were randomly assigned to four study groups (n = 5/group) with washout period of 7 days for recovery and anesthetic withdrawal between experiments. Four study groups were control, nonacupoint electrical stimulation (NA), EA for 30 minutes (SP-6) and continuous EA for 70 or 90 minutes (SP-6C). For the nonacupoint electrical stimulation group, needles were inserted into the nonacupoint at the muscle bellies of left triceps brachii and right quadriceps femoris. MAC and cardiovascular parameters were determined after EA at SP-6 acupoint and at nonacupoint. Thirty minutes of EA and continuous EA until re-determination of MAC at SP-6 acupoint lowered the MAC of isoflurane by 21.3$\pm$8.0% and 16.1$\pm$4.6%, respectively (p<0.05). The decrements in MAC values were not significantly different between two EA groups. However, electrical stimulation of nonacupoint did not induce a significant change in MAC. In SP-6 and SP-6C groups, significant changes in cardiovascular parameters were not observed. These results indicate that EA at SP-6 have an advantage in isoflurane anesthesia in terms of reducing the requirement for anesthetics and minimizing cardiovascular side effects. EA for 30 minutes at maximum might be the sufficient time to produce acupuncture analgesia.

• Journal of Ecology and Environment
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• v.37 no.4
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• pp.285-298
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• 2014

The present study summarizes the occurrence, distribution and autecology of the genus Micrasterias Ralfs among desmids collected from several swamps, reservoirs, rivers and high land wetlands in South Korea from 2009 to 2013. In total 21 taxa of Micrasterias including three new species, Micrasterias spinosus sp. nov., M. jejuensis sp. nov. and M. koreanus sp. nov., were identified. In this study, photomicrographs of all of these are provided and briefly discussed with regard to their taxonomy, distribution and ecology within South Korea.

• Food Science and Preservation
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• v.15 no.2
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• pp.293-299
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• 2008

Bacillus sp. SP-KSW3 is an auxothroph bacteria that is being used for starter in fermentation. Physico-chemical characteristics, enzyme activities, ACE inhibitor and antimutagenicity in fermented soybean (Kanjang) inoculated with Bacillus sp. SP-KSW3 starter was investigated for the ripening duration of fermentation. Tyrosinase and ACE showed 7% higher activity degree on the Kanjang maturated fermented 2 years with Bacillus sp. SP-KSW3 (Type I) than test field than Kanjang maturated 2 years (control). For antimutagenicity using S. enterica serovar Typhimurium TA100 against MNNG and NPD showed 35.17% and 28.37% (Type I). Similarly, S. enterica serovar Typhimurium TA98 was used against NPD and NQO showed 25.48% and 21.64% (Type I), respectively. Hydrogen donating ability 2 year for maturing (Type I) appeared most highly in the test eulogy 83.1% which it makes. Daidzin of isoflavone in fermented soybean showed similarly. Genistein was not detected The initial test field for daidzin and genistein contained 3.95 mg/kg and 1.25 mg/kg (Type I), respectively.

• Korean journal of applied entomology
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• v.40 no.4
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• pp.277-294
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• 2001

Four new and two known species of soil nematodes belonging the order Dorylaimida are described and illustrated. Discolaimium gyeongiens n. sp. is 1.6~1.7 mm long, c=37~41, odontostyle 19~21$\mu\textrm{m}$ long and is characterized by having wider lip region and very short prerectum. Aporcelaimellus donghwaens n. sp. is 2.8~2.9mm long, c=40.0~4.5, odontostyle 19~20$\mu\textrm{m}$ long and is characterized by possessing shorter odontophore, presence of cardiac disc and conoid tail. Labronema korandus n. sp. is 2.2~2.3 mm long, c=68~81, odontostyle 27~28$\mu\textrm{m}$, spicule 61$\mu\textrm{m}$ long and is characterized by having continuous lip region, circular vulva and cylindrical tail. Zalophidera longus n. sp. is 4.1~4.4mm long, c=107~130, spear 80~93$\mu\textrm{m}$ long and is characterized by large sized body, spear and spear extension. Discolaimoides bulbiferous and Diphtherophora communis were reported here for the first time in Korea.

• Korean Journal of Environmental Biology
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• v.22 no.3
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• pp.464-471
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• 2004

A faunal study on the marine decapods of Saemangeum located in the western part of South Korea was performed during the period from August 21, 2003 to October 25, 2003. Forty four species in 17 families were identified through the present investigation, which were represented by 15 species of shrimps in seven families, three species of anomurans in two families, and 26 species of crabs in eight families. Three species of the identified species were new to Korea: Alpheus sp. and Athanas sp. in shrimps, and Asthenognathus sp. in crab. Of the species collected in the present study, Ogyrides orientalis (Stimpson, 1860) in shrimp, and Acmaeopleura balssi Shen, 1932 and Tritodynamia horvathi Nobili, 1905 in crabs are rare species in the western coast of South Korea.