• Title/Summary/Keyword: Sox 9

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Loss of Heterozygosity at the Calcium Regulation Gene Locus on Chromosome 10q in Human Pancreatic Cancer

  • Long, Jin;Zhang, Zhong-Bo;Liu, Zhe;Xu, Yuan-Hong;Ge, Chun-Lin
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.6
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    • pp.2489-2493
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    • 2015
  • Background: Loss of heterozygosity (LOH) on chromosomal regions is crucial in tumor progression and this study aimed to identify genome-wide LOH in pancreatic cancer. Materials and Methods: Single-nucleotide polymorphism (SNP) profiling data GSE32682 of human pancreatic samples snap-frozen during surgery were downloaded from Gene Expression Omnibus database. Genotype console software was used to perform data processing. Candidate genes with LOH were screened based on the genotype calls, SNP loci of LOH and dbSNP database. Gene annotation was performed to identify the functions of candidate genes using NCBI (the National Center for Biotechnology Information) database, followed by Gene Ontology, INTERPRO, PFAM and SMART annotation and UCSC Genome Browser track to the unannotated genes using DAVID (the Database for Annotation, Visualization and Integration Discovery). Results: The candidate genes with LOH identified in this study were MCU, MICU1 and OIT3 on chromosome 10. MCU was found to encode a calcium transporter and MICU1 could encode an essential regulator of mitochondrial $Ca^{2+}$ uptake. OIT3 possibly correlated with calcium binding revealed by the annotation analyses and was regulated by a large number of transcription factors including STAT, SOX9, CREB, NF-kB, PPARG and p53. Conclusions: Global genomic analysis of SNPs identified MICU1, MCU and OIT3 with LOH on chromosome 10, implying involvement of these genes in progression of pancreatic cancer.

A Study on Installation of Removal Device for Volatile Organic Compounds from Petrochemical Plant (석유화학 공장에서 VOCs 제거시설 설치사례 연구)

  • 김석택
    • Journal of environmental and Sanitary engineering
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    • v.15 no.2
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    • pp.95-101
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    • 2000
  • High growth of economy and industrial development was produced every air pollutants, that is, SOx, NOx, dust, VOCs and malodorous gas was produced. Recently, volatile organic compounds(VOCs) was bring about very serious environmental problems. Ulsan petrochemical complex was densely a large scale of petrochemical plant. This study was carried out to select treatment equipment of VOCs produced from petrochemical plant, and has compared with Carbon filter, Regenerative thermal oxidation(RCO) and Flare stack for technical merit and demerit but also initial investment and operating cost.

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Genome-Wide Analysis Reveals Four Novel Loci for Attention-Deficit Hyperactivity Disorder in Korean Youths

  • Kweon, Kukju;Shin, Eun-Soon;Park, Kee Jeong;Lee, Jong-Keuk;Joo, Yeonho;Kim, Hyo-Won
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.29 no.2
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    • pp.62-72
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    • 2018
  • Objectives: The molecular mechanisms underlying attention-deficit hyperactivity disorder (ADHD) remain unclear. Therefore, this study aimed to identify the genetic susceptibility loci for ADHD in Korean children with ADHD. We performed a case-control and a family-based genome-wide association study (GWAS), as well as genome-wide quantitative trait locus (QTL) analyses, for two symptom traits. Methods: A total of 135 subjects (71 cases and 64 controls), for the case-control analysis, and 54 subjects (27 probands and 27 unaffected siblings), for the family-based analysis, were included. Results: The genome-wide QTL analysis identified four single nucleotide polymorphisms (SNPs) (rs7684645 near APELA, rs12538843 near YAE1D1 and POU6F2, rs11074258 near MCTP2, and rs34396552 near CIDEA) that were significantly associated with the number of inattention symptoms in ADHD. These SNPs showed possible association with ADHD in the family-based GWAS, and with hyperactivity-impulsivity in genome-wide QTL analyses. Moreover, association signals in the family-based QTL analysis for the number of inattention symptoms were clustered near genes IL10, IL19, SCL5A9, and SKINTL. Conclusion: We have identified four QTLs with genome-wide significance and several promising candidates that could potentially be associated with ADHD (CXCR4, UPF1, SETD5, NALCN-AS1, ERC1, SOX2-OT, FGFR2, ANO4, and TBL1XR1). Further replication studies with larger sample sizes are needed.

A Study on the Improvement of Dry Bag Filter Treatment System Regarding harmful gas of Glass Recuperator (유리용해로 가스처리 건식 Bag Filter의 개선에 관한 연구)

  • Lee, Sung-Jin;Seo, Man-Chul
    • Journal of environmental and Sanitary engineering
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    • v.23 no.3
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    • pp.9-22
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    • 2008
  • This study was conducted to develop a system that processes harmful gases and dust, which being generated in the production of micro-inorganic fabric. This can be obtained by melt spinning raw materials such as agalmatolite, fluorspar, limestone, silica under high temperature at $1500-1600^{\circ}C$ in a glass recuperator using a dry method by Cyclone Reactor or Envelope Type (ET) type Bag Filter. If the number of the members of Korea Glass Industry Association reaches up to 45, the damage of the harmful gas being generated in recuperator should not be small. In addition, research of existing facilities showed the most of harmful gas treatment facilities which adopt wet treatment or semi-dry treatment process. This was caused the problems for wastewater and the second pollutive materials. Moreover, in the dust collecting facility behind recuperator, it is also problematic that electric dust collector requires enormous initial investment. We have researched various methods to show both economic and efficient new processes for the preventive facilities of recuperator. As the result of the experiments, the removal efficiencies of HF and SOx were 99% and 87%, respectively. Although it was insignificant reaction, a pretty much interesting result that NOx showed an absorption reaction with $Ca(OH)_2$(removal efficiency was more than 25%) was obtained.

A Study on Combustion Process of Biodiesel Fuel with Pilot Injection in a Common-rail Diesel Engine (파일럿분사에 의한 바이오디젤유의 연소과정에 관한 연구)

  • Bang, Joong-Cheol;Kim, Sung-Hoon
    • Transactions of the Korean Society of Automotive Engineers
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    • v.19 no.3
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    • pp.146-153
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    • 2011
  • American NREL (National Renewable Energy Laboratory) reported that BDF20 could reduce PM, CO, SOx, and cancerogenic matters by 13.6%, 9.3%, 17.6%, and 13% respectively, compared to diesel fuel. BDF20 has been being tested on garbage trucks and official vehicles at Seoul City, which is positive on air environment, but negative on combustion by higher viscosity in winter season. This study investigated the combustion characteristics by applying pilot injection for improving the deterioration of combustibility caused by the higher viscosity of the BDF20 with the combustion flames taken by a high-speed camera and the cylinder pressure diagram. A 4-cycle single-cylinder diesel engine was remodeled to a visible 2-cycle engine taking the flame photographs, which has a common-rail injection system. The test was done laboratory temperature at $5{\sim}6^{\circ}C$. The results obtained are summarized as follows, (1) In the case of without pilot injection, the flame propagation speed was slowed and the maximum combustion pressure became lower. The phenomena became further aggravated as the fuel viscosity gets higher. (2) In the case of with pilot injection, early stage of combustion such as rapid ignition timing and flame propagation was activated since intermediate products formed by pilot injection act as a catalyst for combustion of main fuel.

Chondrogenic Differentiation of Porcine Skin-Derived Stem Cells with Different Characteristics of Spontaneous Adipocyte Formation

  • Bae, Hyo-Kyung;Jung, Bae-Dong;Lee, Seunghyung;Park, Choon-Keun;Yang, Boo-Keun;Cheong, Hee-Tae
    • Journal of Embryo Transfer
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    • v.32 no.3
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    • pp.193-200
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    • 2017
  • The purpose of this study is to confirm whether spontaneous adipocyte generation during chondrogenic induction culture affects the chondrogenic differentiation of porcine skin-derived stem cells (pSSCs). For this purpose, chondrogenic differentiation characteristics and specific marker gene expression were analyzed using cell lines showing different characteristics of spontaneous adipocyte formation. Of the four different lines of pSSCs, the pSSCs-IV line showed higher Oil red O (ORO) and glycosaminoglycan (GAG) extraction levels. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed that the levels of adipogenic markers peroxisome proliferator-activated receptor gamma 2 ($PPAR{\gamma}2$) and adipocyte Protein 2 (aP2) mRNAs were significantly higher in pSSCs-IV than those of the other pSSC lines (P<0.05). Among three chondrogenic markers, collagen type II (Col II) and sex determining region Y-box (Sox9) mRNAs were strongly expressed in pSSCs-IV (P<0.05), but not in aggrecan (Agg), which was significantly higher in pSSCs-II (P<0.05). These results demonstrate that the spontaneous adipocyte generation during chondrogenic differentiation has a positive effect on the chondrogenesis of pSSCs. More research is needed on the correlation between adipocyte generation and cartilage formation.

Isopsoralen Induces Differentiation of Prechondrogenic ATDC5 Cells via Activation of MAP Kinases and BMP-2 Signaling Pathways

  • Li, Liang;Eun, Jae-Soon;Nepal, Manoj;Ryu, Jae-Ha;Cho, Hyoung-Kwon;Choi, Bo-Yun;Soh, Yun-Jo
    • Biomolecules & Therapeutics
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    • v.20 no.3
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    • pp.299-305
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    • 2012
  • Endochondral bone formation is the process by which mesenchymal cells condense to become chondrocytes, which ultimately form new bone. The process of chondrogenic differentiation and hypertrophy is critical for bone formation and as such is regulated by many factors. In this study, we aimed to indentify novel factors that regulate chondrogenesis. We investigated the possible role of isopsoralen in induction of chondrogenic differentiation in clonal mouse chondrogenic ATDC5 cells. Isopsoralen treatment stimulated the accumulation of cartilage nodules in a dose-dependent manner. Further, ATDC5 cells treated with isopsoralen were stained more intensely with Alcian blue than control cells, suggesting that isopsoralen increases the synthesis of matrix proteoglycans. Similarly, isopsoralen markedly induced the activation of alkaline phosphatase activity compared with control cells. Isopsoralen enhanced the expressions of chondrogenic marker genes such as collagen II, collagen X, OCN, Smad4 and Sox9 in a time-dependent manner. Furthermore, isopsoralen induced the activation of extracellular signal-regulated kinase (ERK) and p38 MAP kinase, but not that of c-jun N-terminal kinase (JNK). Isopsoralen significantly enhanced the protein expression of BMP-2 in a time-dependent manner. PD98059 and SB 203580, inhibitors of ERK and p38 MAPK, respectively, decreased the number of stained cells treated with isopsoralen. Taken together, these results suggest that isopsoralen mediates a chondromodulating effect by BMP-2 or MAPK signaling pathways, and is therefore a possible therapeutic agent for bone growth disorders.

Peroxiredoxin 3 Has Important Roles on Arsenic Trioxide Induced Apoptosis in Human Acute Promyelocytic Leukemia Cell Line via Hyperoxidation of Mitochondrial Specific Reactive Oxygen Species

  • Mun, Yeung-Chul;Ahn, Jee Young;Yoo, Eun Sun;Lee, Kyoung Eun;Nam, Eun Mi;Huh, Jungwon;Woo, Hyun Ae;Rhee, Sue Goo;Seong, Chu Myong
    • Molecules and Cells
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    • v.43 no.9
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    • pp.813-820
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    • 2020
  • NB4 cell, the human acute promyelocytic leukemia (APL) cell line, was treated with various concentrations of arsenic trioxide (ATO) to induce apoptosis, measured by staining with 7-amino-actinomycin D (7-AAD) by flow cytometry. 2', 7'-dichlorodihydro-fluorescein-diacetate (DCF-DA) and MitoSOX™ Red mitochondrial superoxide indicator were used to detect intracellular and mitochondrial reactive oxygen species (ROS). The steady-state level of SO2 (Cysteine sulfinic acid, Cys-SO2H) form for peroxiredoxin 3 (PRX3) was measured by a western blot. To evaluate the effect of sulfiredoxin 1 depletion, NB4 cells were transfected with small interfering RNA and analyzed for their influence on ROS, redox enzymes, and apoptosis. The mitochondrial ROS of NB4 cells significantly increased after ATO treatment. NB4 cell apoptosis after ATO treatment increased in a time-dependent manner. Increased SO2 form and dimeric PRX3 were observed as a hyperoxidation reaction in NB4 cells post-ATO treatment, in concordance with mitochondrial ROS accumulation. Sulfiredoxin 1 expression is downregulated by small interfering RNA transfection, which potentiated mitochondrial ROS generation and cell growth arrest in ATO-treated NB4 cells. Our results indicate that ATO-induced ROS generation in APL cell mitochondria is attributable to PRX3 hyperoxidation as well as dimerized PRX3 accumulation, subsequently triggering apoptosis. The downregulation of sulfiredoxin 1 could amplify apoptosis in ATO-treated APL cells.

Avenanthramide C as a novel candidate to alleviate osteoarthritic pathogenesis

  • Tran, Thanh-Tam;Song, Won-Hyun;Lee, Gyuseok;Kim, Hyung Seok;Park, Daeho;Huh, Yun Hyun;Ryu, Je-Hwang
    • BMB Reports
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    • v.54 no.10
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    • pp.528-533
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    • 2021
  • Osteoarthritis (OA) is a degenerative disorder that can result in the loss of articular cartilage. No effective treatment against OA is currently available. Thus, interest in natural health products to relieve OA symptoms is increasing. However, their qualities such as efficacy, toxicity, and mechanism are poorly understood. In this study, we determined the efficacy of avenanthramide (Avn)-C extracted from oats as a promising candidate to prevent OA progression and its mechanism of action to prevent the expression of matrix-metalloproteinases (MMPs) in OA pathogenesis. Interleukin-1 beta (IL-1β), a proinflammatory cytokine as a main causing factor of cartilage destruction, was used to induce OA-like condition of chondrocytes in vitro. Avn-C restrained IL-1β-mediated expression and activity of MMPs, such as MMP-3, -12, and -13 in mouse articular chondrocytes. Moreover, Avn-C alleviated cartilage destruction in experimental OA mouse model induced by destabilization of the medial meniscus (DMM) surgery. However, Avn-C did not affect the expression of inflammatory mediators (Ptgs2 and Nos) or anabolic factors (Col2a1, Aggrecan, and Sox9), although expression levels of these genes were upregulated or downregulated by IL-1β, respectively. The inhibition of MMP expression by Avn-C in articular chondrocytes was mediated by p38 kinase and c-Jun N-terminal kinase (JNK) signaling, but not by ERK or NF-κB. Interestingly, Avn-C added with SB203580 and SP600125 as specific inhibitors of p38 kinase and JNK, respectively, enhanced its inhibitory effect on the expression of MMPs in IL-1β treated chondrocytes. Taken together, these results suggest that Avn-C is an effective candidate to prevent OA progression and a natural health product to relieve OA pathogenesis.

Differentiation potential of canine mesenchymal stem cells on hydrogel scaffold-based three-dimensional environment (하이드로젤 지지체 기반 3차원 환경에서 개 간엽줄기세포의 분화능 분석)

  • Gu, Na-Yeon;Park, Mi Jeong;Lee, Jienny;Byeon, Jeong Su;Jeong, Da-Un;Cho, In-Soo;Cha, Sang-Ho
    • Korean Journal of Veterinary Research
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    • v.58 no.4
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    • pp.211-217
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    • 2018
  • Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy. Physiological cell environment not only connects cells to each other, but also connects cells to the extracellular matrix that provide mechanical support, thus exposing the entire cell surface and activating signaling pathways. Hydrogel is a polymeric material that swells in water and maintains a distinct 3-dimensional (3D) network structure by cross linking. In this study, we investigated the optimized cellular function for canine adipose tissue-derived MSCs (cAD-MSCs) using hydrogel. We observed that the expression levels of Ki67 and proliferating cell nuclear antigen, which are involved in cell proliferation and stemness, were increased in transwell-hydrogel (3D-TN) compared to the transwell-normal (TN). Also, transforming growth factor-${\beta}1$ and SOX9, which are typical bone morphogenesis-inducing factors, were increased in 3D-TN compared to the TN. Collagen type II alpha 1, which is a chondrocyte-specific marker, was increased in 3D-TN compared to the TN. Osteocalcin, which is a osteocyte-specific marker, was increased in 3D-TN compared to the TN. Collectively, preconditioning cAD-MSCs via 3D culture systems can enhance inherent secretory properties that may improve the potency and efficacy of MSCs-based therapies for bone regeneration process.