Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Peroxiredoxin 3 Has Important Roles on Arsenic Trioxide Induced Apoptosis in Human Acute Promyelocytic Leukemia Cell Line via Hyperoxidation of Mitochondrial Specific Reactive Oxygen Species

  • Mun, Yeung-Chul (Department of Hematology and Oncology, Ewha Womans University College of Medicine) ;
  • Ahn, Jee Young (Department of Hematology and Oncology, Ewha Womans University College of Medicine) ;
  • Yoo, Eun Sun (Department of Pediatrics, Ewha Womans University College of Medicine) ;
  • Lee, Kyoung Eun (Department of Hematology and Oncology, Ewha Womans University College of Medicine) ;
  • Nam, Eun Mi (Department of Hematology and Oncology, Ewha Womans University College of Medicine) ;
  • Huh, Jungwon (Department of Laboratory Medicine, Ewha Womans University College of Medicine) ;
  • Woo, Hyun Ae (Graduate School of Pharmaceutical Sciences, Ewha Womans University) ;
  • Rhee, Sue Goo (Yonsei Biomedical Research Institute, Yonsei University College of Medicine) ;
  • Seong, Chu Myong (Department of Hematology and Oncology, Ewha Womans University College of Medicine)
  • Received : 2019.10.15
  • Accepted : 2020.08.25
  • Published : 2020.09.30
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Abstract

NB4 cell, the human acute promyelocytic leukemia (APL) cell line, was treated with various concentrations of arsenic trioxide (ATO) to induce apoptosis, measured by staining with 7-amino-actinomycin D (7-AAD) by flow cytometry. 2', 7'-dichlorodihydro-fluorescein-diacetate (DCF-DA) and MitoSOX™ Red mitochondrial superoxide indicator were used to detect intracellular and mitochondrial reactive oxygen species (ROS). The steady-state level of SO2 (Cysteine sulfinic acid, Cys-SO2H) form for peroxiredoxin 3 (PRX3) was measured by a western blot. To evaluate the effect of sulfiredoxin 1 depletion, NB4 cells were transfected with small interfering RNA and analyzed for their influence on ROS, redox enzymes, and apoptosis. The mitochondrial ROS of NB4 cells significantly increased after ATO treatment. NB4 cell apoptosis after ATO treatment increased in a time-dependent manner. Increased SO2 form and dimeric PRX3 were observed as a hyperoxidation reaction in NB4 cells post-ATO treatment, in concordance with mitochondrial ROS accumulation. Sulfiredoxin 1 expression is downregulated by small interfering RNA transfection, which potentiated mitochondrial ROS generation and cell growth arrest in ATO-treated NB4 cells. Our results indicate that ATO-induced ROS generation in APL cell mitochondria is attributable to PRX3 hyperoxidation as well as dimerized PRX3 accumulation, subsequently triggering apoptosis. The downregulation of sulfiredoxin 1 could amplify apoptosis in ATO-treated APL cells.

Keywords

References

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