• Proceedings of the KSLP Conference
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• 2003.11a
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• pp.183-183
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• 2003

Background and Objectives : Cartilage reconstruction is one of medical issue in otolaryngology. Tissue engineering is presently being utilized in part of cartilage repair. Sources of cells for tissue engineering are chondrocyte from mature cartilage and bone marrow mesenchymal stem cells that are able to differentiate into chondrocyte. Recent studies have shown that adipose tissue have mesenchymal stem cells which can differentiate into adipogenic, chondrogenic myogenic osteogenic cells and neural cell in vitro. In this study, we have examined chondrogenic potential of the canine adipose tissue-derived mesenchymal stem cell(ATSC). Materials and Methods : We harvested canine adipose tissue from inguinal area. ATSCs were enzymatically released from canine adipose tissue. Under appropriate culture conditions, ATSCs were induced to differentiate into the chondrocyte lineages using micromass culture technique. We used immunostain to type II collagen and toluidine blue stain to confirm chondrogenic differentiation of ATSCs. Results : We could isolate ATSCs from canine adipose tissue. ATSCs expressed CD29 and CD44 which are specific surface markers of mesenchymal stem cell. ATSCs differentiated into micromass that has positive response to immunostain of type II collagen and toluidine blue stain. Conclusion : In vitro, ATSCs differentiated into cells that have characteristic cartilage matrix molecules in the presence of lineage-specific induction factors. Adipose tissue may represent an alternative source to bone marrow-derived MSCs.

• Applied Biological Chemistry
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• v.35 no.1
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• pp.30-35
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• 1992

Two strains J2W and J2Y which were isolated from soil can utilize polyvinyl alcohol(PVA) as a sole carbon source. PVA was utilized symbiotically by the mixed culture of these two strains which could not utilize PVA in each respective pure culture. Effect of degree of PVA polymerization on the its utilization was examed, and there was remarkable difference among three kind of PVA(PVA 500, 1500 and 2000). The reconstruction of there two strains was carried out with other symbionts Pseudomonas sp. PW and Pseudomonas sp. G5Y which were able to utilize PVA. PVA utilization occured in each remixed culture of J2Y strain with Pseudomonas sp. PW J2W strain with Pseudomonas sp. G5Y, respectively. Identification of bacteria was based on morphological and biological chatacteristics, J2W and J2Y strain were similar to a strain of Pseudomonas pseudimallei and Xanthomonas campestris, respectively.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2004.10a
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• pp.26-31
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• 2004

This work was undertaken in order to study the developmental competence of nuclear transfer cat embryo with fetal fibroblast and adult skin fibroblast as donor nuclei. Oocytes wererecovered by mincing the ovaries in Hepes-buffered TCM199 and selected the cumulus oocyte complexes (COCs) with compact cumulus cell mass and dark. Homogenous ooplasm were cultured for maturation in TCM199 + 10% fetal bovine serum (FBS) for 12 hours and used as a source of recipient cytoplast for exogenous somatic nuclei. In Experiment 1, we evaluated the effect donor cell types on the reconstruction and development of cloned embryos. Fusion, first cleavage and blastocyst developmental rate was not different between fetal fibroblast and adult skin cell (71.2 vs. 66.8; 71.0 vs. 57.6; 4.0 vs. 6.1 %, P<0.05). In Experiment 2, cloned embryos were surgically transferred into the oviducts of recipient queens. One of seven recipient queens was delivered naturally 2healthy cloned cats and 1 stillborn from fetal fibroblast cell of male origin after 65 days embryo transfer. One of three recipient queens was delivered naturally 1 healthy cloned cat from adult skin cell of female after 65 days embryo transfer. The cloned cats showed genotypes identical to the donor cell lines, indicating that adult somatic cells can be used for feline cloning.

• Korean Journal of Geomatics
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• v.5 no.1
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• pp.7-19
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• 2005

Photogrammetric mapping procedures have gone through major developments due to significant improvements in its underlying technologies. The availability of GPS/INS systems greatly assist in direct geo-referencing of the acquired imagery. Still, photogrammetric datasets taken without the aid of positioning and navigation systems need control information for the purpose of surface reconstruction. Point features were, and still are, the primary source of control for the photogrammetric triangulation although other higher-order features are available and can be used. LIDAR systems supply dense geometric surface information in the form of three dimensional coordinates with respect to certain reference system. Considering the accuracy improvement of LIDAR systems in the recent years, LIDAR data is considered a viable supply of photogrammetric control. To exploit LIDAR data, new challenges are poised concerning the representation and reference system by which both the photogrammetric and LIDAR datasets are described. In this paper, registration methodologies will be devised for the purpose of integrating the LIDAR data into the photogrammetric triangulation. Such registration methodologies have to deal with three issues: registration primitives, transformation parameters, and similarity measures. Two methodologies will be introduced that utilize straight-line and areal features derived from both datasets as the registration primitives. The first methodology directly incorporates the LIDAR lines as control information in the photogrammetric triangulation, while in the second methodology, LIDAR patches are used to produce and align the photogrammetric model. Also, camera self-calibration experiments were conducted on simulated and real data to test the feasibility of using LIDAR patches for this purpose.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.5
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• pp.81-101
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• 2011

This study was conducted to understand the environmental and ecological function of habitat through evaluation of water environment, soil environment, vegetation characteristics, macro-invertebrate characteristics, and visual habits environment evaluation (SVAP) in Hongdong stream located in Hongseong-Gun, Chungnam Province, and hereafter to utilize the results for the habits reconstruction and improvement project. As the results of water quality analysis, BOD, COD, T-P was almost below the standard quality from upper stream to down stream. The construction of small reservoir, wetland and water purification facility, and the management of non-point pollution are proposed to improve these problems. The soil texture was sandy soil, which is unfitted with vegetation development. The construction of shallows and bogs, and induction of soil sedimentation and biotope formation are proposed to improve these problems. In the plant flora, total 90 kinds were observed with 81 species and nine varieties in total 36 families and 85 genera, and the naturalization rate was higher at down area than upper area. As the results of macro-invertebrate fauna survey, total 26 species and 297 individuals in 20 families and 22 genera were collected. Peltodytes sinensis, Chironomidae sp., and Culicidae sp., which are observed at polluted environment, were collected as dominant species. An appropriate vegetation management party idea is necessary, and it is done an idea in consideration of the soil and a physical characteristic. Visual habits environment evaluation (SVAP) result was mostly determined with below normal (Fair) grade. Pollution source interception, purification pond establishment, and various bog establishment are proposed to improve these problems. With the above results, the ecological environment was determined with bad condition, and the improvement of biotope was urgently needed through sustainable monitoring and management of streams habitat in rural area.

• Journal of Radiation Protection and Research
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• v.47 no.2
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• pp.93-98
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• 2022

Background: This study aims to derive the characteristics of each work type for industrial radiography based on empirical evidence through expert advice and a survey of radiation workers of various types of industrial radiography. Materials and Methods: According to a Korean report, work types of industrial radiography are classified into indoor tests, underground pipe tests, tests in a shielded room (radiographic testing [RT] room test), outdoor field tests, and outdoor large structure tests. For each work type, exposure geometry and radiation sources were mainly identified through the expert advice and workers' survey as reliable empirical evidence. Results and Discussion: The expert advice and survey results were consistent as the proportion of the work types were high in the order of RT room test, outdoor large structure test, underground pipe test, outdoor field test, and indoor test. The outdoor large structure test is the highest exposure risk work type in the industrial radiography. In most types of industrial radiography, radiation workers generally used ¹⁹²Ir as the main source. In the results of the survey, the portion of sources was high in the order of ¹⁹²Ir, X-ray generator, ⁶⁰Co, and ⁷⁵Se. As the exposure geometry, the antero-posterior geometry is dominant, and the rotational and isotropic geometry should be also considered with the work type. Conclusion: In this study, through expert advice and a survey, the external exposure characteristics for each work type of industrial radiography workers were derived. This information will be used in the reconstruction of organ dose for health effects assessment of Korean radiation workers.

• The Journal of Society for e-Business Studies
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• v.11 no.1
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• pp.1-23
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• 2006

As data on the Web is more and more in XML due to proliferation of Web applications such as e-Commerce, it is strongly required to rapidly process XML queries. One of such techniques is XML query caching. For frequently submitted queries, their results could be cached in order to guarantee fast response for the same queries. In this paper, we propose techniques for XML query performance improvement whereby the set of node identifiers(NIS) for an XML query is cached. NIS is most commonly employed as a format of XML query result,, consisting of the identifiers of the XML elements that comprise the query result. With NIS, it is suitable to meet the Web applications data retrieval requirements because reconstruction and/or modification of query results and integration of multiple query results can be efficiently done. Incremental refresh of NIS against its source updates can also be efficiently done. When the query result is requested in XML, however, materialization of NIS is needed by retrieving the source XML elements through their identifiers. In this paper, we consider three different types of NISs. proposing the algorithms of their creation, materialization, and incremental refresh. All of them were implemented using an RDBMS. Through a detailed set of performance experiments, we showed the efficiency of the proposed XML query caching techniques.

• Restorative Dentistry and Endodontics
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• v.36 no.2
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• pp.98-107
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• 2011

Caries remains prevalent throughout modern society and is the main disease in the field of dentistry. Although studies of this disease have used diverse methodology, recently, X-ray microtomography has gained popularity as a non-destructive, 3-dimensional (3D) analytical technique, and has several advantages over the conventional methods. According to X-ray source, it is classified as monochromatic or polychromatic with the latter being more widely used due to the high cost of the monochromatic source despite some advantages. The determination of mineral density profiles based on changes in X-ray attenuation is the principle of this method and calibration and image processing procedures are needed for the better image and reproducible measurements. Using this tool, 3D reconstruction is also possible and it enables to visualize the internal structures of dental caries. With the advances in the computer technology, more diverse applications are being studied, such automated caries assessment algorithms.

• Progress in Medical Physics
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• v.19 no.4
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• pp.209-218
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• 2008

According to improved radiation therapy technology such as IMRT and proton therapy, the accuracy of patient alignment system is more emphasized and IGRT is dominated research field in radiation oncology. We proposed to study the feasibility of cone-beam CT system using simple x-ray imaging systems for image guided proton therapy at National Cancer Center. 180 projection views ($2,304{\times}3,200$, 14 bit with 127 ${\mu}m$ pixel pitch) for the geometrical calibration phantom and humanoid phantoms (skull, abdomen) were acquired with $2^{\circ}$ step angle using x-ray imaging system of proton therapy gantry room ($360^{\circ}$ for 1 rotation). The geometrical calibration was performed for misalignments between the x-ray source and the flat-panel detector, such as distances and slanted angle using available algorithm. With the geometrically calibrated projection view, Feldkamp cone-beam algorithm using Ram-Lak filter was implemented for CBCT reconstruction images for skull and abdomen phantom. The distance from x-ray source to the gantry isocenter, the distance from the flat panel to the isocenter were calculated as 1,517.5 mm, 591.12 mm and the rotated angle of flat panel detector around x-ray beam axis was considered as $0.25^{\circ}$. It was observed that the blurring artifacts, originated from the rotation of the detector, in the reconstructed toomographs were significantly reduced after the geometrical calibration. The demonstrated CBCT images for the skull and abdomen phantoms are very promising. We performed the geometrical calibration of the large gantry rotation system with simple x-ray imaging devices for CBCT reconstruction. The CBCT system for proton therapy will be used as a main patient alignment system for image guided proton therapy.

• Progress in Medical Physics
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• v.17 no.4
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• pp.212-223
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• 2006

Positional accuracy of the on-board imager (OBI) isocenter with gantry rotation was presented in this paper. Three different type of automatic evaluation methods of discrepancies between therapeutic and OBI isocenter using digital image processing techniques as well as a procedure stated in the customer acceptance procedure (CAP) were applied to check OBI isocenter migration trends. Two kinds of kV x-ray image set obtained at OBI source angle of $0^{\circ},\;90^{\circ},\;180^{\circ},\;270^{\circ}$ and every $10^{\circ}$ and raw projection data for cone-beam CT reconstruction were used for each evaluation method. Efficiencies of the methods were also estimated. If a user needs to obtain an isocenter variation map with full gantry rotation, a method taking OBI image for every $10^{\circ}$ and fitting with 5th order polynomial was appropriate. However for a mere quality assurance (QA) purpose of OBI isocenter accuracy, it was adequate to use only four OBI Images taken at the OBI source angle of $0^{\circ},\;90^{\circ},\;180^{\circ}\;and\;270^{\circ}$. Maximal discrepancy was 0.44 mm which was observed between the OBI source angle of $90^{\circ}\;and\;180^{\circ}$ OBI isocenter accuracy was maintained below 0.5 mm for a year. Proposed QA program may be helpful to Implement a reasonable routine QA of the OBI isocenter accuracy without great efforts.