• Applied Biological Chemistry
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• 제37권2호
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• pp.85-93
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• 1994

연속식 2단계 막(MWCO 10,000, MWCO 5,000)반응기를 이용하여 어피젤라틴 가수분해물을 제조하여 그 가수분해물의 분자량, 아미노산조성 및 기능성에 대하여 검토하였다. 1단계 젤라틴 가수분해물의 분자량은 $8{\sim}10\;KDa$ 및 $4.5{\sim}6.5\;KDa$이 주종을 이루었으며, 2단계 가수분해물의 분자량은 $2{\sim}6\;KDa$ 및 2 KDa 이하의 저분자 펩타이드도 존재하였다. 어피젤라틴의 아미노산조성과 1단계 및 2단계 어피젤라틴 가수분해물의 아미노산조성 사이에는 거의 차이가 없었고, 감칠맛과 단맛에 관련이 있는 아미노산 함량이 전체의 $68{\sim}72%$에 달한 반면, 쓴맛을 내는 아미노산 함량은 $23{\sim}25%$에 불과하였으며, 1단계 가수분해물에 비해 2단계 가수분해물이 단맛과 감칠맛이 더 좋았다. 1단계 및 2단계 가수분해물의 용해도는 모든 pH영역에서 완전히 용해하였으나, 유화성 및 포말성은 거의 나타나지 않았다. 완충능은 2단계 가수분해물이 가장 높았으나, 점도는 모든 pH영역에서 거의 차이가 없었으며, 어피젤라틴 보다는 가수분해물의 점도가 더 낮았다. 그리고 2단계 가수분해물은 등온흡습도가 가장 높아 수분활성 저하제로서 이용할 수 있을 것으로 판단되었다.

• 한국초등과학교육학회지:초등과학교육
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• 제22권2호
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• pp.138-148
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• 2003

This study was to analyze how elementary school science textbooks explain dissolution and to examine the patterns of elementary school teachers' conceptions on dissolution and the teaching practices on dissolution of elementary school teachers. According to the result of the textbook analysis, the textbooks based on the 7th curriculum didn't explain dissolution very differently from those based on the 6th curriculum. The contents dealing with dissolution in the textbooks of 7th curriculum became difficult gradually as the year went up, but the connected organization of the contents made students learn it easily. For example, in order to learn dissolution introduced first in the 3rd year 2nd semester textbook, students would tell soluble substance in water from insoluble substance in water as they put powdered substance in water. In the 5th year 1st semester textbook students were supposed to acquire the knowledge related to dissolution through the designed activities such as comparing solubility produced by different solvents and defining a solvent, a solute, dissolution, and a solution. In addition, teachers' guide for 5th year 1st semester textbook elucidated the principle of dissolution using attraction concept that was scientific. The result of the survey on teachers showed that 90% of elementary school teachers understood the dissolution of salt in water just as millet particles' filling the space between bean particles and they responded that they demonstrated millet particles' filling the space between been particles when they taught the dissolution of salt in water. When it comes to teachers who had the right idea on dissolution as the attraction conception, understanding was one thing and teaching was another, because they often instructed dissolution as the space conception in the real teaching.

• 치위생과학회지
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• 제21권4호
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• pp.243-250
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• 2021

Background: Endodontic sealers or their toxic components may become inflamed and lead to delayed wound healing when in direct contact with periapical tissues over an extended period. Moreover, an overfilled sealer can directly interact with adjacent tissues and may cause immediate necrosis or further resorption. Therefore, the treatment outcome conceivably depends on the endodontic sealer's biocompatibility and osteogenic potential. This study aimed to evaluate the cell viability and osteogenic effects of four different sealers in osteoblastic cells. Methods: AH Plus (resin-based sealer), Pulp Canal Sealer EWT (zinc oxide-eugenol sealer), BioRoot RCS (calcium silicate-based sealer), and Well-Root ST (MTA-based calcium silicate sealer) were mixed strictly according to the manufacturer's instructions, and dilutions of sealer extracts (1/2, 1/5 and 1/10) were determined. Cell viability was measured using the water-soluble tetrazolium-8 (WST-8) assay. Differentiation was assessed by alkaline phosphatase (ALP) activity and mineralized nodule formation by Alizarin Red S staining. Results: The cell viability of the extracts derived from the sealers excluding Well-Root ST was concentration dependent, with sealer extracts having the least viability at a 1/2 dilution. At sealer extract dilution of 1/10, the test groups showed the same survival rate as that control group, with the exception of BioRoot RCS. Among all experimental groups, BioRoot RCS showed the highest cell viability after 48 hours. The ALP activity was significantly higher in a concentration-dependent manner. Furthemore, all four materials promoted ALP activity and mineralized nodule formation compared to the control at 1/10 dilutions. Conclusion: This is the first study to highlight the differences in biological activity of these four materials. These results suggest that the composition of root canal sealers appears to alter the form of biocompatibility and osteoblastic differentiation.

• 아시안잔디학회지
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• 제1권1호
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• pp.56-68
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• 1987

During the regrowth the process after mowing, NRA in the leaf was the highest activity from the 5th day to the 7th day. Before mowing, the NRA in the root was not almost detected. But, the NRA in root appeared a rapid increasing activity from the 3rd day to the 4th day after mowing ( Figs.27 ~ 32). During the regrowth process after mowing, a general tendency of AA in the aboveground parts appeared an increasing tendency from the 1st day to the 4th day, a rapid increasing tendency from the 7th day to the 8th day reaching its peak, and a decreasing rate on the 8th and 9th day reaching its peak, and a decreasing rate on the 8th and 9th day. But the AA in the root appeared rapid increasing rate from the 2nd day to the 7th day, the heginning of reagrowth, this tendency showed a similar figure in the case of Total Soluble Carbohydrate ( TSC) in the internode. Both AA and NRA were appeared recovery stage frorn the 8th day after mowing(Figs.15~20). During the regrowth process after mowing, changes of the maximum plant lengths were 18.27cm in the 6cm mowing plot on the 24th day after mowing, 18.83cm in the 3cm mowing plot on the 18th day after mowing, and 18.16cm in the 6cm mowing plot on the 14th day after mowing ( Fig.2). During the regrowth process after mowing. changes in Dry Matter (DM) contents in leaf and stem were a slow decreasing tendency from the 1st day to the 4th day. From the 5th day to the 8th day it appeared a rapid increasing tendency. And afterward until the 15th day. All treatments were reached at a steady state ( Figs.3 ~ 8). During the regrowth process after mowing, changes in the TSC contents of stem and crown were a slow decreasing tendency from the 1st day to the 5-6th day. Prom the 7th to the 8th day three was a rapid increasing tendency. And afterward until the 15th day there was a decreasing rate at a steadyv state. In root there was a similar tendency to that of leaf and stem organs. A general tendency in internode, the TSC content appeared a similar figure to increment of AA (Figs. 9 - 14). During the regrowth process after mowing, changes in te Crude Proem (CP) content of ahoveground parts appeared a slow increasing tendency from the 1st day to the 5-6th day, where it is peak. And afterward to the 15th day there was a decreasing rate at a steady state. But, in toot there were a contrary tendency to that of aboveground

• 동아시아식생활학회지
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• 제8권2호
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• pp.116-125
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• 1998

This study was designed to find out the anti-cancer effects of two herbs using a folk remedy in Korea-The two herbs were A: Equisetum arvense L. (Seddugi), B: Eunymus alatus Siebold. (Hwasalnamoo) The experiment for the anti-cancer effects were conducted in mice and experimental groups were divided into normal(NO), standard (ST) and two other(A,B) groups for each herb and each of the two experimental groups consisted of three sub-groups(I, II, III) according to three different quantity doses of a folk remedy using five times and ten times the quantity of a commonly used in a folk remedy. The water soluble extracts of two herbs were orally administered to mice during six weeks followed by inoculation of the sarcoma-180 tumor cells into the peritoneal cavity. During experimental periods, the contents of ascites, abdominal organs were observed histopathologically. The results of effects on anticancer were as follows : 1. The survival time was significantly prolonged by the two herbs, especially in groups AII & BII.2. Body weight, circumference of the abdomen and contents of ascites at the time of sacrifice were noted and significantly decreased in groups AI, AII & BII. 3. Anti-cancer effects evaluated by the invasiveness and thickness of the tumor cells were noted in groups AIII & BII.

• 한국미생물·생명공학회지
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• 제24권3호
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• pp.274-281
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• 1996

A bacterium producing Cyclodextrin Glucanotransferase (CGTase) and Cyclodextrinase (CDase) was isolated from soil, and named as Bacillus stearothermophilus KJ16. The growth of the isolated strain occurred in two steps, and syntheses of CGTase and CDase were dependedt on the growth cycle of the cell. CGTase was constitutively synthesized during the 1st growing phase, while CDase was synthesized inducibly during the 2nd growing phase. When the midium pH was controlled at 7.0 the maximum enzyme activities of CGTase and CDase were increased by 12-fold (1300 mU/ml) and 2-fold (225 mU/ml), respectively, compared with the pH-uncontrolled batch culture. The CGTase of the isolate converted soluble starch to CDs with the ratio of $\alpha$-CD:$\beta$-CD:$\gamma$-CD=42:46:12 at $55^{\circ}C$.The optimal pH and temperature of CGTase were 6.0 and $60^{\circ}C$, respectively and the optimal pH and temperature of CDase were 6.0 and $55^{\circ}C$. The molecular weights of the purified CGTase and CDase were estimated to be 65, 000 and 68, 000 dalton, respectively. Among several substrates, $\gamma$-CD was most rapidly hydrolyzed by the purified CDase.

• 한국식품영양학회지
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• 제7권4호
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• pp.274-283
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• 1994

To provide the efficient application scheme of buckwheat dietary fiber and basic information of seed germination, buckwheat(Fagopyrum esculentum Moench) was germinated at 10$^{\circ}C$ for 7 days and the contents and composition of the total dietary fiber(TDF), insoluble dietary fiber(IDF), soluble dietary fiber(SDF) wire examined at 24 hour intervals. The TDF content in ungerminated seeds was 24.86o on dry weight basis. It decreased for the 1st day of germination, but gradually increased for 7 days afterwords. The contents of IDF and SDF in ungerminated seeds were 22.05, 1.42% respectively. The IDF and SDF contents decreased in the initial stage of germination, but then gradually increased. The composition of the IDF and SDF in the TDF during the germination period showed different tendencies. The IDF decreased with germination time until 5 days and then increased. The SDV increased until 5 days and then decreased gradually. The TDF contents obtained by AOAC method were generally higher than those obtained by Prosky method. The TDF contents obtained by the two method, however, were very closely correlated (r=0.9966, p< 0.01) The IDF(X1) and SDF(X2) showed the significant regression equation(p<0.01) with the root length(Y). The equation was Y: -12.6681+0.5089${\times}$ 1 $\div$ 0.6022Xa and R2 was 0.968.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제19권3호
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• pp.265-286
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• 1997

Bone morphogenetic proteins(BMPs) are a group of transforming growth factor beta(TGF-${\beta}$)-related factors and multifunctional proteins, especially the only known biologic factors capable of inducing endochondral bone formation at an extraskeletal site. This study was performed to investigate the effect of the partially purified porcine BMP(pBMP) at an ectopic site. PBMP was partially purified from porcine bone matrix and its activity was monitored by an in vivo bioassay. The purification method utilized extraction of the bone-inducing activity with 4M guanidine, followed by chromatography on heparin-Sepharose. Active fractions were assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. And the fractions were reconstituted with inactive insoluble collagenous bone matrix from rats, acid soluble type I collagen from rat tail and chondroitin-6-sulfate sodium salt and implanted into the pectroralis muscle pouches of Sprague-Dawley rats. And the carrier complex was implanted on the opposite side as control. The rats were sacrificed at the day of 1st, 3rd, 5th, 7th, 11th, 14th and 21st after implantation and examined histologically, radiologically and biochemically. And alkaline phosphatase activity and calcium content were used as indices of bone formation. The results were as follows ; 1. Active fractions were localized in a zone between 31 and 40 KDa on SDS-PAGE. 2. The implanted 3.0mg of the partially purified pBMP induced cartilage and bone in the muscle tissue of rats through an endochondral ossification process. 3. Inactive insoluble bone matrix, type I collagen and chondroitin-6-sulfate have functioned as carriers for pBMP, but revealed some foreign body reactions. 4. Soft X-ray didn't reveal significant change between the experimental and the control group. 5. The alkaline phosphatase activities in the experimental group of 5th, 7th, 11th, 14th and 21st were increased significantly compared with control (p<0.01) with the peak in the group of 11th day. 6. With time, the calcium content of the experimental group increased. And the calcium contents in the experimental group of 11th, 14th and 21st were increased significantly compared with control (p<0.01).

• 한국초지조사료학회지
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• 제24권3호
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• pp.183-192
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• 2004

Orchardgrass 및 white clover에서 미량요소 Fe, Mn, Cu 및 Zn의 systematic variation 시비가 목초의 생육, 개화, 수량 및, 양분 함량 등에 미치는 영향 등을 구명하였다. 다량요소 양분을 동일 양 시비한 조건에서 Fe/Cu(시험군-1), Mn/Zn(시험군-2) 및 Fe+Cu/Mn+Zn(시험군-3)의 처리는 systematic variation 방법으로 시험군별 총 시비량을 각각 0/100, 25/75, 50/50, 75/25, $100/0\%$ 비율로 나누어 시비하였고, Fe/Mn/Cu/Zn(시험군-4)에서는 각 기준처리 $70\%$, 기타 처리는 각각 $10\%$(합계 $100\%$) 비율로 시비하였다. 1. N-화합물들의 함량은 일반적으로 수량과 반비례적인 경향을 보였다. Mn/Zn 비율시험에서 white clover는 0/100 처리에서 큰 수량감소와 더불어 수용성 N-화합물 함량이 다소 높았고(특히 3차 예취), 또한 순단백질/수용성 N-화합물 비율은 낮았다. 2. Fe+Cu/Mn+Zn 비율시험에서 white clover는 0/100과 100/0 처리에서 이들 함량들이 다소 높았다. 이는 큰 수량감소와 이와 연관된 농축효과에 기인된 것으로 보였다. 또한 white clover는 5차 예취 시 100/0 처리에서 큰 수량 감소와 더불어 수용성 N-화합물 함량이 상대적으로 더 높아졌다. 이 결과 순단백질/수용성 N-화합물 비율이 더 낮아졌다. 100/0 처리는 white clover의 단백질 함성 생리대사에 부정적인 영향을 준 것으로 보였다. 3. Fe/Mn/Cu/Zn 비율시험에서 white clover는 특히 1차 예취에서 조단백질의 함량이 매우 낮았다. 이는 순단백질 함량이 상대적으로 크게 낮은 것과 연관되었고, 아마도 단일 양분의 다량 시비비율에 따른 이들 양분간 불균형에 따른 것으로 보였다. 4. 처리별 K, Ca 및 Mg의 함량은 일반적으로 차이가 경미하였으나 5차 예취에서 white clover는 Mn/Zn=0/100과 Fe+Cu/Mn+Zn=100/0처리에서 K-함량이 상대적으로 높은 수준을 보였다. 이는 Mn-결핍에 따른 white clover의 수량감소와 이와 연관된 농축효과에 기인된 것으로 보였다.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.503-509
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• 1998

Whole faba beans (WFB) were dry roasted at different temperatures of 110, 130 and $150^{\circ}C$ for 15, 30 and 45 minutes (min) to determine the optimal heating conditions to increase bypass starch as glucose source which may be a limiting nutrient in high producing dairy cattle. Ruminant degradation characteristics of starch (St) of WFB were determined using in sacco method in 6 dairy cows fed 60% hay and 40% concentrate. Measured characteristics of St were soluble (washable) fraction (S), potentially degradation fraction (D) and the rate of degradation (Kd) of the insoluble but degradable St fraction. Based on measurement of these characteristics, percentage bypass starch (%BSt) and bypass starch (BSt) were calculated. Degradability of starch in the rumen was reduced by dry roasting at temperature of 130 and $150^{\circ}C$ and increased at $110^{\circ}C$. S varied from 50.0% in the raw whole faba beans (RWFB) and 53.7% in $110^{\circ}C$/15 min to 18.2% in $150^{\circ}C$/45 min. D varied from 49.9% in RWFB and 46.3% in $110^{\circ}C$/15 min to 81.8 % in $150^{\circ}C$/45 min. Kd varied from 9.8% in RWFB and 11.0% in the $110^{\circ}C$/30 min to 4.2 in $150^{\circ}C$/45 min. All these effects resulted in increasing %BSt from 22.1% in the $110^{\circ}C$/45 min and 23.9% in RWFB to 49.9% in the $150^{\circ}C$/45 min. Therefore BSt increased from 91.4 g/kg and 98.4 g/kg to 199.9 g/kg respectively. Dry roasting at $110^{\circ}C$ increased the starch rumen degradation. Treatment at higher temperature (130 and $150^{\circ}C$) decreased rumen degradation of starch and seemed to be linear up to highest values tested. No optimal dry roasting conditions of treatment could be determined at this stage. It may be concluded that dry roasting at temperatures of 130 and $150^{\circ}C$ was effective in shifting starch degradation from rumen to intestine to increase bypass starch.