• Title/Summary/Keyword: Soil-root system

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Root metabolic cost analysis for root plasticity expression under mild drought stress

  • Kano-Nakata, Mana;Mitsuya, Shiro;Inukai, Yoshiaki;Yamauchi, Akira
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.328-328
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    • 2017
  • Drought is a major limiting factor that reduces rice production and occurs often especially under recent climate change. Plants have the ability to alter their developmental morphology in response to changing environment, which is known as phenotypic plasticity. In our previous studies, we found that one chromosome segment substitution line (CSSL50 derived from Nipponbare and Kasalath crosses) showed no differences in shoot and root growth as compared with the recurrent genotype, Nipponbare under non-stress condition but showed greater growth responses compared with Nipponbare under mild drought stress condition. We hypothesized that reducing root respiration as metabolic cost, which may be largely a consequence of aerenchyma formation would be one of the key mechanisms for root plasticity expression. This study aimed to evaluate the root respiration and aerenchyma formation under various soil moisture conditions among genotypes with different root plasticity. CSSL50 together with Nipponbare and Kasalath were grown under waterlogged conditions (Control) and mild drought stress conditions (20% of soil moisture content) in a plastic pot ($11cm{\times}14cm$, ${\varphi}{\times}H$) and PVC tube ($3cm{\times}30cm$, ${\varphi}{\times}H$). Root respiration rate was measured with infrared gas analyzer (IRGA, GMP343, Vaisala, Finland) with a closed static chamber system. There was no significant difference between genotypes in control for shoot and root growth as well as root respiration rate. In contrast, all the genotypes increased their root respiration rates in response to mild drought stress. However, CSSL50 showed lower root respiration rate than Nipponbare, which was associated by higher root aerenchyma formation that was estimated based on internal gas space (porosity) under mild drought stress conditions. Furthermore, there were significant negative correlations between root length and root respiration rate. These results imply that reducing the metabolic cost (= root respiration rate) is a key mechanism for root plasticity expression, which CSSL50 showed under mild drought.

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A Study on Test Methods for Performance Appraisal of Root Barrier Appling to Green Roofs (옥상 및 인공지반녹화용 방근재의 성능기준 설정을 위한 방근성 시험방법에 관한 연구)

  • Oh, Sang-Keun;Kwak, Kyu-Sung;Sun, Yoon-Suk;Kwon, Shi-Won
    • Journal of the Korea Institute of Building Construction
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    • v.7 no.1 s.23
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    • pp.79-84
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    • 2007
  • Selection of proper root barrier as destination part of greening is very important in Root penetration resistance plan. To select proper root barrier, it need to understand composition of greening part, size, kind of plant, connection with waterproofing layer. In this point of view, we have establish greening on the roof or concrete structure, not been understand the structural mechanism. It means that we misunderstood about purpose of greening and using it. So, chosen materials and construction method was not proper for greening, it caused water leakage and decrease performance of concrete structure. Therefore, we would suggest 5 items of test methods considering environmental condition for green roof. Watertightness by water of greening part, root penetration resistance test by root penetration, bacteria resistance by must or bacteria in soil, chemical resistance by rain and chemical agent of fertilizer, and load resistance by soil depth, size of plant. These suggested test methods could be referred as guideline to test in green roof system because of not exist any performance appraisal guideline or standard. Consequently, it should be analysis as technical and institutional subdividing test methods and it need to study constantly as varied angles.

Isolation and Characteristics of Exopolysaccharide Producing Bacteria in a Ginseng Root System (인삼 근계로부터 다당 생성세균의 분리 및 특성)

  • Cho, Geon-Yeong;Jeon, In-Hwa;Han, Song-Ih;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.49 no.3
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    • pp.297-300
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    • 2013
  • EPS producing bacteria were enumerated in ginseng root system (rhizosphere soil, rhizoplane, inside of root). EPS producing bacterial density of rhizosphere soil, rhizoplane and inside of root were distributed $9.0{\times}10^6$ CFU/g, $7.0{\times}10^6$ CFU/g, and $1.4{\times}10^3$ CFU/g, respectively. Phylogenetic analysis of the 24 EPS producing isolates based on the 16S rRNA gene sequences, EPS producing isolates from rhizosphere soil (RS) belong to genus Arthrobacter (6 strains) and Rhizobium (1 strain). EPS producing bacteria from rhizoplane (RP) were Arthrobacter (6 strains), Rhodococcus (1 strain) and Pseudomonas (1 strain). EPS producing bacteria from inside of root (IR) were categorized into Rhzobium (6 strains), Bacillus (1 strain), Rhodococcus (1 strain), and Pseudomonas (1 strain). Phylogenetic analysis indicated that Arthrobacter may be a member of representative EPS producing bacteria from ginseng rhizosphere soil and rhizoplane, and Rhizobium is typical EPS producing isolates from inside of ginseng root. The yield of EPS was 10.0 and 4.9 g/L by Rhizobium sp. 1NP2 (KACC 17637) and Arthrobacter sp. 5MP1 (KACC 17636). The purified EPS were analyzed by Bio-LC and glucose, galactose, mannose and glucosamine were detected. The major EPS sugar of these strains was glucose (72.7-84.9%).

Review of Studies on Ginseng Replanting Problems (인삼 연작장해 연구에 대한 고찰)

  • 이종철;김홍진;오승환
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.s02
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    • pp.115-120
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    • 1989
  • Universal characteristics of ginseng replanting problems appeared to be decrease in root yields due to root rot and inhibition of root growth. Incitants of ginseng replanting problems have not been clearly elucidated, however, it appeared to be a complex phenomenon with several pathogenic soil microorganisms and environmental changes in the soil due to decomposition of ginseng debris such as rootlets and shoots. Decomposition of ginseng debris may cause direct or indirect damage to the root. The effect of conventional fungicide on the control of the problems has not been recognized. However, it has been suggested that treatment with soil fumigants may solve the problems. Meanwhile paddy-rice and ginseng rotation system appeared to be the best way of solving the problems so far.

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Development of Self-propelled Explosive Subsoiler (2) - Construction of Prototype and Performance Evaluation - (자주식 심토환경 개선기 개발(2) - 본체 제작 및 성능 평가 -)

  • Lee, Dong-Hoon;Park, Woo-Pung;Kim, Sang-Cheol;Lee, Kyou-Seung
    • Journal of Biosystems Engineering
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    • v.34 no.6
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    • pp.404-410
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    • 2009
  • This study was carried out to develop a self-propelled type explosive subsoiler for improving the root zone soil conditions in orchard and other forest fields. Prototype was designed to be able to inject air and other soil improving material such as lime into soil at the same time, and thus improve the air permeability and drainage of orchard soils to promote the root growth of tree for high quality fruit production. Soil penetration device of explosive subsoiler is composed of air hammer, penetration rob and air injection nozzle. To support the soil penetration device of explosive subsoiler to penetrate vertically, modified Scott-Russel mechanism was used. Timing control device for simultaneous injection of soil improving material with air was attached to the out side wall of air cylinder and as the cylinder move, the soil improving material was injected into soil at the same time. Turning radius of prototype was 2.2-2.3 m with good mobility in sloped land. It took approximately 1 minute for lime injection system to reach the optimum pressure of 9.9 kg/$cm^2$, average 10-20 seconds were required to rupture soil with the depth of 50 cm and 2-3 seconds were required for explosion, so all in all about 1 minute and 20 seconds were required for one cycle of explosion. Maximum soil rupture depth and diameter were 50 cm and 3-4 m respectively depending on the soil type and soil moisture content. For final design of explosive subsoiler inclination angle of lime hopper was increased from 60 degree to 70 degree and the shape of hopper was changed from rectangular cone to circular cone to solve the clogging problem of lime at out let. Agitating system operated by compressed air was attached to the metering device of the prototype, thus more than 90 cc of lime was discharged per cycle from metering device without clogging problems.

The Growth Rate of Salix Gracilistyla Miq. and its Effect of Protecting Soil from Dispersion Depending on the Planting Method Applied to Shore-marginal Slope (습지 수제부에서 삽목방법에 따른 갯버들 생장율 및 토양 유실 억제 효과)

  • Lee, Chun-Seok;Ryu, Nam-Hyung
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.6 no.3
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    • pp.56-68
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    • 2003
  • The main purpose of this study was to verify the shore margin protection effect of the root system of Salix gracilistyla Miq. developed from direct sticking cuttings on wetland, through the measurement of root growth and comparison of soil slaking rate depending on the planting method applied to shore-marginal slope. Comparison of growth rate and soil dispersion rate was made between five planted slope and one naked slope. The planting methods applied to the planted slope were (a) horizontally layed burying of stick(45cm) bundle (b) horizontally layed covering the slope with sticks (c) horizontally fencing with normal cuttings(20cm) (d) elected sticking of normal cutting at equal distances (e) random scattering short cuttings(3-4cm). As results, the most effective planting method was horizontally layed burying, and in order to increase its efficiency scattering the live stem chips in 2-3cm on the slope is recommended. The growth of root was negatively regressive to the distance from water floor.

Diversity and Phylogenetic Analysis of Fluorescent Pseudomonads Isolated from Soil-Root System of Red Pepper in Greenhouse (비닐하우스 고추재배지의 토양과 근계로부터 분리된 형광성 Pseudomonads의 계통 분류 및 다양성)

  • Kwon, Soon-Wo;Kim, Jong-Shik;Song, Jae-Kyeong;Ryu, Jin-Chang
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.4
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    • pp.275-282
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    • 2000
  • Among the fluorescent pseudomonad isolates from soil- root system of red pepper in Chinju, Kyunsangnam-Do, the phylogenetic analysis for 35 isolates were conducted. The partial 16S ribosomal DNA sequences were used as taxonomic key for phylogenetic analyses, and these sequences were enabled to identification of the fluorescent pseudomonad isolates on the species level. The 17 isolates among them were classified into Pseudomonas putida group, and consisted of the strains isolated mainly from soil. This group were subdivided into 4 subgroups (I, II, III, and IV). The subgroup I and IV were unique ones which were relatively remotely related with subgroup II and III including the type strain of P. putida. The 15 isolates among 35 isolates were grouped along with the type strain of Pseudomonas fluorescens, and 3 isolate were characterized as intermediates of P. fluorescens and Pseudomonas chlororaphis. Most of strain isolateds from the rhizosphere soil and rhizoplane of red pepper were identified as P. fluorescens and closely related with each other. In this study, root of red pepper was supposed to be colonized by a specific strain or strains of P. fluorescens.

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Effects of localised liquid fertilization of N, P, K and Ca on root development in Zoysia matrella, Cynodon dactylon and Stenotaphrum secundatum

  • Ow, Lai Fern;Yusof, Mohamed Lokman Mohd
    • Weed & Turfgrass Science
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    • v.7 no.1
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    • pp.76-86
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    • 2018
  • Turfgrass species were evaluated for their rooting and foliar characteristics, and their interaction with the soil. The rooting system was divided into three compartments, one above another, such that the top and bottom compartments of the root system could be supplied with a nutrient deprived solution. Exposure of parts of the roots to nitrate deprivation caused a localised retardation of root initiation and extension, compared with zones receiving the full supply of nutrients. This resulted in considerable modification to root form, coupled with a significant depression in foliar growth. The extension of roots was the least affected by the deprivation of potassium. Phosphate and calcium deprivations gave rise to similar responses in root and foliar formation. Results from this study showed that external concentrations of nitrogen, phosphorus, potassium and calcium are required by the root system in varying amounts for optimal growth of roots. Turfgrass coverage and turf quality ratings further reinforced these findings. No significant difference was observed between the different grasses examined here. All three species responded similarly to the deprivation of the various nutrients. Results from this study confirmed that targeted fertilization programs are beneficial and can help reduce cost, chemical usage and prevent leachate and contamination.

Estimation of Soil Cooling Load in the Root Zone of Greenhouses (온실내 근권부의 지중냉각부하 추정)

  • 남상운
    • Journal of Bio-Environment Control
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    • v.11 no.4
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    • pp.151-156
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    • 2002
  • Root zone cooling, such as soil or nutrient solution cooling, is less expensive than air cooling in the whole greenhouse and is effective in promoting root activity, improving water absorption rate, decreasing plant temperature, and reducing high temperature stress. The heat transfer of a soil cooling system in a plastic greenhouse was analyzed to estimate cooling loads. The thermal conductivity of soil, calculated by measured heat fluxes in the soil, showed the positive correlation with the soil water content. It ranged from 0.83 to 0.96 W.m$^{[-10]}$ .$^{\circ}C$$^{[-10]}$ at 19 to 36% of soil water contents. As the indoor solar radiation increased, the temperature difference between soil surface and indoor air linearly increased. At 300 to 800 W.m$^{-2}$ of indoor solar radiations, the soil surface temperature rose from 3.5 to 7.$0^{\circ}C$ in bare ground and 1.0 to 2.5$^{\circ}C$ under the canopy. Cooling loads in the root zone soil were estimated with solar radiation, soil water content, and temperature difference between air and soil. At 300 to 600 W.m$^{-2}$ of indoor solar radiations and 20 to 40% of soil water contents,46 to 59 W.m$^{-2}$ of soil cooling loads are required to maintain the temperature difference of 1$0^{\circ}C$ between indoor air and root zone soil.

Effects of Soil Moisture Control and Dormancy Breaking Agents on Bud Burst and Fruiting for Double Cropping System in a Year in 'Kyoho' Grapes (포도 '거봉' 2기작재배를 위한 하계휴면타파에서 토양수분 조절과 휴면타파제 처리가 발아에 미치는 영향)

  • 오성도;김용현;최동근
    • Journal of Bio-Environment Control
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    • v.11 no.2
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    • pp.61-66
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    • 2002
  • Double cropping system in a year in Kyoho grapes (Vitis labruscana L.) has currently been attempted in the plastic greenhouse. One of the problems in double cropping system is the promotion of bud break in summer season and shoot fertility. Effects of the control of soil moisture tension near the root zone and treatments of bud dormancy breaking agents on bud breaking in summer were examined to promote the bud break for the second fruiting. The lignification of shoots was induced in July or August by the control of soil moisture tension in root zone environment. The first shoot growth was almost the same as that in common plastic greenhouses. The highest bud break value appeared in the plot of cyanamide chemicals mixed with merit blue as over 75% bud break rate. The bud break rate in the discontinuing plot in irrigation showed significantly higher in bud break than that in the continuing plot in irrigation. Despite of the final high bud break rate, the time of bud break was irregular.