• Journal of Soil and Groundwater Environment
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• v.25 no.2_spc
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• pp.16-27
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• 2020

Nitrate contamination has received much attention at local as well as regional scales. The domestic situation is not out of exception, and it has been reported to be very serious, particularly within agricultural areas as a result of excessive usage of nitrogen fertilizers. Meanwhile, nitrate can be naturally attenuated by denitrification in subsurface environments. The denitrification occurs through biotic (biological) and abiotic processes, and numerous previous studies preferentially focused the former. However, abiotic denitrification seems to be significant in specific environments. For this reason, this study reviewed the previous studies that focused on abiotic denitrification processes. Firstly, the current status of nitrate contamination in global and domestic scales is presented, and then the effect of geological media on denitrification is discussed while emphasizing the significance of abiotic processes. Finally, the implications of the literature review are presented, along with future research directions that warrant further investigations. The results of previous studies demonstrated that several geological agents could play a vital role in reducing nitrate. Iron-containing minerals such as pyrite, green rust, magnetite, and dissolved ferrous ion are known to be powerful electron donors triggering denitrification. In particular, it was proven that the rate of denitrification by green rust was comparative to that of biological denitrification. The results indicate that abiotic denitrification should be taken into account for more accurate evaluation of denitrification in subsurface environments.

• Journal of the Korean Society of Groundwater Environment
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• v.6 no.3
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• pp.107-110
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• 1999

18 boreholes with nitrate contaminated were selected. Samples were collected 4 times between both 1995 and 1996. Stable nitrogen isotope ratio for them all was measured and the contribution to contamination from several sources like fertilizer, sewage, cropland. and landfill was analysed. Nitrogen source for 11 sampling sites of T-3, L-1. O-1∼O-4, F-2∼F-5, and G-2 considered to come from chemical fertilizer and its contribution was around 60% or more. T-4. T-5 were located downward the downtown, which were influenced bydomestic sewage and its contribution were 70.7% and 54.7%. Nitrate concentration of G-2 was 17.7 mg/L, among which 60.7% was estimated to come from landfill leachate. T-1 and T-2 were located in the small village, in which 42.2 and 43.4% of nitrogen was to come from domestic sewage but 52.8% and 56.0% were from fertilizer sprayed in the cropland. L-2 was near livestock by which it was estimated to be influenced, in which 59.9% of nitrogen was from cropland. F-1 was in the cropland, by which 50.0% was influenced and 49.5% was estimated from organic matter of animals.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.435-441
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• 2004

A drum bioreactor was used for the treatment of sandy soil contaminated with three kinds of aromatic compounds (phenol, naphthalene, and phenanthrene), and its performance was evaluated in two different operation modes; intermittent and continuous rotation of drum. When the drum bioreactor was operated with one rotation per day, the microbial growth was relatively low, and most of the compounds remaining in soil, except naphthalene of 90 mg/kg dry soil, disappeared mainly due to volatilization. In contrast, when the drum was continuously rotated at 9 rpm (rotation/min), the number of microorganisms was drastically increased and nitrate was consumed for growth as a nitrogen source. Phenol and phenanthrene were removed at rates of 56.7 mg/kg dry soil/day and 3.2 mg/kg dry soil/day, respectively.

• Korean Journal of Environmental Agriculture
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• v.24 no.2
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• pp.132-138
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• 2005

Objective of this research was to evaluate the fate of nitrogen and phosphorous in hydroponic waste solution from the plastic film house cultivation applied to the upland soil by column leaching and field experiment. The pH and EC of leachate were decreased by the reaction with the upland soil in the column leaching experiment. The EC and concentrations of $H^+,\;K^+,\;and\;{NH_4}^+$ of leachate were decreased as the column length (soil depth) was increased. But these were increased as the amounts of the hydroponic waste solution were increased field experiment growing red pepper (Capsicum annum L.) to monitor the nutrients movement using ion exchange resin capsule demonstrated that the nutrient concentration of soil solution was increased in the orders of $PO_4-P. Nitrate concentration of resin capsule inserted into the soil was relatively higher than other nutrients $(NH_4-N\;and\;PO_4-P)$ at the 45 cm of soil depth. The overall results demonstrated that the hydroponic waste solution could be recycled as plant nutrients to enhance fertility of soils. But nitrate leaching was a major factor for safe use of the hydroponic waste solution in soil.

• Ecology and Resilient Infrastructure
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• v.2 no.3
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• pp.247-254
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• 2015

Changes in land-use type can affect soil and water properties in stream ecosystems. This study examined the effects of different land-use types on biogeochemical properties and microbial activities of a stream. We collected water and sediment samples in a stream at three different sites surrounded by varying land-use types; a forest, a radish field and a rice paddy. Nitrogen contents, such as nitrate, nitrite and total nitrogen in the stream water body, showed significant differences among the sampling sites. The highest nitrogen values were recorded at the site surrounded by cropland, as fertilizer runoff impacted the stream. Soil organic matter content in the sediment showed significant differences among sites, with the highest content exhibited at the forest mouth site. These differences might be due to the organic matter in surrounding terrestrial ecosystems. Microbial activities determined by extracellular enzyme activities showed similar values throughout all sites in the water body; however, the activities in the sediments exhibited the highest values near the forest site and mirrored the soil organic matter content values. From these results, we conclude that different land-use types are important factors affecting water and sediment properties in stream ecosystems.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.3
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• pp.158-164
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• 2001

The purpose of this study was to assess the runoff of nitrogen derived from pig manure under upland condition. Bare and maize cultivated conditions were compared to estimate the effect of plant on the runoff loss of nitrogen and other nutrients by application of pig manure. Soil used in this experiment was sandy loam, and the fermented pig manure was applied at the rate of 0, 50, and $100ton\;ha^{-1}$. The amount of runoff was measured after every rainfall and water samples were analyzed for nitrate and other cations. Runoff was increased with the rainfall, but was depended on the application rate of pig manure at both bare and maize cultivated plots. Concentrations of nitrate in runoff at 0, 50 and $100ton\;ha^{-1}$ application of pig manure were higher at the maize cultivated plots than those at bare plots by 86.9, 42.9, and 33.6%, respectively. However, total mass of nitrate by runoff loss was higher at the bare plot ranging from 1.34 to $3.15kg\;NO_3-N\;ha^{-1}$. The equivalent ratio of nitrate to sum of cations in runoff was higher at the bare plot than that of maize cultivated plots. The concentration of cations in runoff was in the order of K> Mg> Na> Ca.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.4
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• pp.407-412
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• 1985

The effect of potash applied with different sources of nitrogen was experimented in pot soil culture on chinese cabbage (Brassica Pekinensis Rupr, Var. Fall 1984: Sammi Garak, Spring 1985: Jungang Summer lab.) in the fall, 1984 and in the spring, 1985. Results obtained are as follows; 1. Ammonium nitrate increased the yield of chinese cabbage more than urea did, and the effect of yield increase by ammonium nitrate was greater in the fall cultivation than in the spring. 2. The yield of chinese cabbage was positively correlated with the contents of K in the first (May 17, 1985) and second (June 9, 1985) thined cabbages (dry matter). It was also positively correlated with $NO_3-N$ content of the first thined cabbage and with K/Ca+Mg m.e, ratio of outer leaves of the harvested (June 27, 1985) cabbage, but negatively affected with Mg content of the outer leaves. 3. Correlations between K and $NO_3-N$ contained in the dry matter of first and second thined, and inner leaves of the harvested cabbage were learnt to be $r;0.9998^{**}$, r;0.4439, and $r;-0.7135^*$ respectively. The higher $NO_3-N$ content in the inner leaves of harvested cabbage was observed at K omitted ammonium nitrate plot where K was deficient, Ca and Mg contents were low. 4. The nutrient absorption and growth of chinese cabbage may take the following process. Nitrate nitrogen increases vegetative growth of the plant with enhanced K uptake and movement in to inner leaves and followed by replacement of Ca uptake and finally Mg uptake and its movement in to inner leaves.

• Journal of Soil and Groundwater Environment
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• v.10 no.3
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• pp.9-15
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• 2005

Soil aquifer treatment is a water reuse technology that secondary or tertiary treated wastewater is infiltrated into the aquifer in which physical and biochemical reactions occur. Major consideration in SAT is the removal and transport of DOC and nitrogen species. In this study, reaction mechanism in SAT was examined considering nitrification, denitrification and organic oxidation. In addition, SAT modeling system was developed as the reaction mechanism was applied to groundwater flow and transport model. In verification of the reaction module by 1-dimensional unsaturated soil column test, the experimental data of all of the species, ammonium, nitrate, DOC and DO, were well matched with the simulation results. In sensitivity analysis, ammonium partition coefficient, dissolved oxygen inhibition constant and biomass decay rate affect ammonium, DOC and DO concentration of effluent, respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.1
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• pp.32-37
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• 2006

In this study, we have purified and characterized the membrane bound nitrate reductase obtained from the denitrifying bacteria, Ochrobactrum anthropi SY509, which was isolated from soil samples. O. anthropi SY509 can grow in minimal medium using nitrate as a nitrogen source. We achieved an overall purification rate of 15-fold from the protein extracted from the membrane fraction, with a recovery of approximately 12% of activity. The enzyme exhibited its highest level of activity at pH 5.5, and the activity was increased up to $70^{\circ}C$. Periplasmic and cytochromic proteins, including nitrite and nitrous oxide reductase, were excluded during centrifugation and were verified using enzyme essay. Reduced methyl viologen was determined to be the most efficient electron donor among a variety of anionic and cationic dyestuffs, which could be also used as an electron donor with dimethyl dithionite. The effects of purification and storage conditions on the stability of enzyme were also investigated. The activity of the membranebound nitrate reductase was stably maintained for over 2 weeks in solution. To maintain the stability of enzyme, the cell was disrupted using sonication at low temperatures, and enzyme was extracted by hot water without any surfactant. The purified enzyme was stored in solution with no salt to prevent any significant losses in activity levels.

• Korean Journal of Environmental Agriculture
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• v.16 no.4
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• pp.295-303
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• 1997

To investigate the transformation characteristics of nitrogen and carbon from cow manure compost amended in soil under different moisture conditions, dynamics of nitrogen and carbon were determined periodically for 15 weeks of aerobic incubation at room temperature during July${\sim}$November, 1996. Cow manure compost matured with mixing saw dust was amended with the 4 ratios (0, 2, 4, 6%(wt/wt)) in Ap horizon soil, which collected from green house in Yesan, Chungnam. Moisture was controlled with 0.2, 0.3, 0.4, and 0.5 of mass water conte nt (${\theta}$m) to air dried soil, and water loss was compensated at every sampling. During incubation, soil pH was decreased continuously, that was caused by hydrogen generated from nitrification of ammonium nitrogen. And pH became higher with inclining cow manure compost amendment and water treatment, that meaned the increase of mineralization of organic-N to $NH_4\;^+-N$. Total nitrogen was reduced with increasing water content, but total carbon showed the contrast tendency with that of nitrogen. Therefore, C/N ratio slightly decreased in the low water condition (${\theta}$m 0.2) during incubation, but increased continuously in high water condition over ${\theta}$m 0.4. As a result, it was assumed that soil fertility is able to be reduced in the high water content over available water content. Nitrate transformation rate increased lasting in the low water content less than ${\theta}$m 0.3. Itdropped significantly in the first $2{\sim}3$ weeks of incubation over ${\theta}$m 0.4. In particular, nitrate was not detected in ${\theta}$m 0.5 of water content after the first $2{\sim}3$ weeks. In contrast, ammonium transformation was inclined with increasing water treatment. Nitrogen mineralization rate, which calculated with percentage ratio of (the sum of ex.$NH_4\;^+-N$ and $NO_3\;^--N$)/total nitrogen, was continuously increased in the low water content of ${\theta}$m 0.2 and 0.3. But it saw the different patterns in high water content over ${\theta}$m 0.4 that was drastically declined in the initial stage and then gradually inclined . From the above results, nitrogen transformation patterns differentiated decisively in water content between ${\theta}$m 0.3 and 0.4 in soil. Thus, it is very important for the maintain of suitable soil water content to enhance fertility of soil amended with manure compost. However, excess treatment of manure compost might enhance the possibility of contamination of small watershed and ground water around agricultural area.