• Microbiology and Biotechnology Letters
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• v.23 no.1
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• pp.36-42
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• 1995

Actinomycetes occur in a wide range of environments and many more actinomycetes remain to be detected in the natural environment. In isolation stages, selection of the environment as a source of useful isolates is important. Two hundred and eighteen strains were isolated on Bennet's agar and 346 strains were on humic acid-vitamin agar from five each paddy field, field, forest, grass land, riverside soil samples. These isolates were identified to the genus level based on morphological and physiological characteristics. Among them, 386 strains were Streptomyces, 49 strain were Nocardia, 35 strains were Microbispora and Micromonospora each, 15 strains were Nocardiopsis, 13 strains were Actinomadura, 10 strains were Streptosporangium, and the others were isolated rarely. According to soil type, Nocardia, Micromonospora, Microbispora and Streptosporangium were dominant in paddy field, Microbispora Nocardia, Nocardioides and Micromonospora were dominant in field, Nocardia, Micromonospora, Microbispora and Actinomadura were dominant in grass land, Nocardia, Micromonospora and Microbispora were dominant in forest, Nocardia, Microbispora and Micromonospora were dominant in riverside. Generally, Nocardia, Micromonospora, Microbispora and Actinomadura were isolated in all kinds of soils, Streptosporangium were paddy field, Dactylosporangium were forest, Nocardiopsis were field, forest and riverside.

• The Korean Journal of Pesticide Science
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• v.8 no.3
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• pp.198-209
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• 2004

In order to elucidate the behavior of the fungicide hexaconazole in soil and rice plants, rice plants were grown for 42 days in a microecosystem (pot) containing fresh and 28 day-aged soil residues of $[^{14}C]$hexaconazole. The amount of $^{14}CO_2$ evolved during 28 days of aging was 0.11 % of total $^{14}C$-radioactivity treated and the averaged weekly degradation rate was 0.03%. Mineralization rates for 42 days of rice cultivation on fresh and aged paddy soils were 0.67% of the total $^{14}C$ in case of non-rice planting on aged soil and 1.17% in case of rice planting on aged soil, whereas 1.25% in non-rice planting on fresh soil and 1.72% in case of rice planting on fresh soil, suggesting that the amounts of $^{14}CO_2$ were evolved higher from fresh soils than aged ones and from rice-planting soils than non-planting ones. The amounts of volatiles collected were very low as background levels. Most of $^{14}C$-Radioactivity was remained in soil after 42 days of rice cultivation and $^{14}C$ absorbed through rice roots was distributed more in shoots than roots and translocated into the edge of shoots of rice plants. Amounts of non-extractable $^{14}C$ in soils were higher in rice planted soil than in non-planting soil. The distribution of non-extractable $^{14}C$ was increased in the order of humin>fulvic acid>humic acid. The amounts of $^{14}C$ translocated into rotational crop Chinese cabbage were 2.36 and 3.69% of the total $^{14}C$ in case of rice planted soil containing fresh and aged residues, respectively, suggesting that small amounts of $[^{14}C]$hexaconazole and its metabolite(s) were absorbed and their bound residues were more available than their fresh ones to Chinese cabbage.

• Journal of the Mineralogical Society of Korea
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• v.19 no.4 s.50
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• pp.231-238
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• 2006

Transports of heterogeneously charged particles were investigated based on column experiments. Synthesized mono-dispersed ferrihydrite (${\sim}100nm$) and amorphous $SiO_2\;({\sim}40nm\;and\;{\sim}80nm)$ particles, of which surfaces are oppositely charged under pH < 9.0 (ferrihydrite, positive; amorphous silica, negative), were used. $177{\sim}250{\mu}m$ quartz sand was used as a stationary matrix. The results show that even favorable particles (i.e., ferrihydrite) can show a conservative transport through the oppositely charged media (i.e., quartz) when they coexist with humic acid or with much greater number of oppositely charged particles. These results imply that transports of both negatively and positively charged contaminants may be possible at the same time under a condition of heterogeneous colloidal system.

• Weed & Turfgrass Science
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• v.7 no.3
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• pp.259-268
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• 2018

This study was conducted to evaluate the effect of liquid fertilizer containing humic acid and Saccharomyces cerevisiae broth (LHS) on changes of turfgrass growth by investigating visual quality, chlorophyll content, dry weight of clipping, and nutrient content in leaf tissues. Treatments were designed as follows; control fertilizer (CF), HS-1 ($CF+1.0mL\;m^{-2}\;LHS$), HS-2 ($CF+2.0mL\;m^{-2}\;LHS$), and HS-3 ($CF+4.0mL\;m^{-2}\;LHS$). After treatment of LHS on creeping bentgrass, soil pH in the treated plots was decreased than that of CF. As compared to CF, visual quality, chlorophyll content and content of N, P and K were not significantly different in the LHS treatments. However, clipping yield and phosphorus uptake of HS-2 were significantly increased by 22% and 33%, respectively. These results showed that application of LHS improved the phosphorus uptake and growth of creeping bentgrass, which would be an alternative management tool for the cool season turfgrass under stress conditions.

• Weed & Turfgrass Science
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• v.6 no.3
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• pp.272-281
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• 2017

This study was conducted to evaluate the effect of liquid fertilizer containing humic acid (LFHA) on changes of creeping bentgrass quality and growth. Treatments were designed as follows; control fertilizer (CF), HA-1 ($CF+1ml\;m^{-2}LFHA$ LFHA), HA-2 ($CF+2ml\;m^{-2}LFHA$ LFHA), and HA-3 ($CF+4ml\;m^{-2}LFHA$). As compared with CF, soil chemical properties and chlorophyll content of clipping of LFHA treatments were not significantly different. Visual quality in both of HA-2 and HA-3 treatments was higher than that of CF treatment from September to November, and clipping yield on October 27. Shoot density, root length, and the content of nitrogen, phosphorus or potassium were increased by application of LFHA. The clipping yield was positively correlated with phosphorus content, potassium content or shoot density. Similarly, LFHA level was proportionate to clipping yield of creeping bentgrass, and N, P, K contents in the leaf tissue. These results generally demonstrated that the application of LFHA improved the growth and quality of creeping bentgrass by increase of N content or P in leaf tissue.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.4
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• pp.273-277
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• 2001

Traditional methods of chemical analysis for the soil properties take time and produce harmful waste. The purpose of this research was to evaluate an NIR technique for measuring some soil properties that are rapid and accurate in soil fertility assessments. The NIR instrument (InfraAlyzer 500, Bran & Luebbe Co.) was used for obtaining spectral data from 140 finely ground soil for calibrations and validation estimating pH, CEC, extractable Ca, Mg, K, $SiO_2$, humic acid and EC. Partial least square regression analysis was used to develop a calibration of NIR spectroscopy method. The results indicated that NIR spectroscopy could be used as a routine nondestructive method quantitatively determining soil chemical properties quickly. However the NIR technique may require sample preparation to obtain even diffuse reflection spectra from the soil and data manipulations to obtain optimal predictions.

• Journal of Wetlands Research
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• v.22 no.4
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• pp.328-336
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• 2020

In this study, the soil characteristics of peat layer in Korea were analysed to predict new peat distribution areas in Taean, Chungcheongnam-do. The soil characteristics of peat distribution areas in Taean were compared with those in other areas in Korea. In Korea, peat researches were conducted in various regions, such as Taean in Chungcheongnam-do, Pyeongtaek in Gyeonggi Province, Gangneung in Gangwon-do, Ulsan, Iksan in Jeollabuk-do and Jangheung in Jeollanam-do. We summarized a) topographic distribution, b) deposition type, c) soil drainage classes, and d) soil suborder of previously studied area to determine criteria for prediction of peat distribution area. Through a case study, the type of peat layer distribution area in Taean were divided into two; (1) a) valley, b) co-alluvium, c) somewhat poorly drained, d) aqualfs and (2) a) marine plain, b) alluvium, c) very poorly drained, d) aquepts. A field survey was conducted to confirm the distribution of the actual peat layer. All peat layer were located within 10-km from the coastal line which is similar to previous studies, and the peat layer was characterized with pH 5, humic acid content of less than 15% and dark brown color. The new peat layer in Taean was deeper, thicker and less humificated than other studied areas.

• Journal of Soil and Groundwater Environment
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• v.9 no.3
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• pp.12-19
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• 2004

Binding mechanisms of polycyclic aromatic hydrocarbons (PAHs) with a purified Aldrich humic acid (PAHA) and its ultrafiltration (UF) size fractions were investigated. Organic carbon normalized binding coefficient ($K_oc$) values were estimated by both a conventional Stern-Volmer fluorescence quenching technique and a modified fluorescence quenching method. Pyrene $K_oc$ values depended on PAHA concentration as well as freely dissolved pyrene concentration. Such nonlinear sorption-type behaviors suggested the existence of specific interactions. Smaller molecular size PAH (naphthalene) exhibited higher $K_oc$ value with medium-size PAHA UF fractions whereas larger size PAH (pyrene) had higher extent of binding with larger PAHA UF fractions. The inconsistent observation for naphthalene versus pyrene was well explained by size exclusion effect, one of the previously suggested specific mechanisms for PAH binding. In general, the extent of pyrene binding increased with lower pH likely due to the neutralization of acidic functional groups in HS and the subsequent increase in hydrophobic HS region. However, pyrene $K_oc$ results with a large UF fraction (>100K Da) corroborated the existence of the size exclusion effect as demonstrated by an increase in $K_oc$ values at a certain higher pH range. The size exclusion effect appears to be effective only for the specific conditions (HS size or pH) that render HS hole st겨ctures to fit a target PAH.

• Journal of Technologic Dentistry
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• v.22 no.1
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• pp.145-152
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• 2000

In order to select an effective antibiotic substance against oral resident bacteria, we were isolated from soil and texonomically analyzed. Seven hundred and eighteen strains were isolated on humic acid- vitamin agar(HV agar) and 220 strains were on methanol medium from three each paddy forest, field and riverside soil samples. So, during the screening of antibiotics from soil, we isolated microorganisms showing powerful antagonistic activity against oral resident bacteria. Microorganism was tested against 25 strains of bacteria, yeast and fungi. Among them, No. 248 strain exhibited the most strongly growth inhibition. So, the taxonomical analysis the isolated strain was found to be unknown Actinomyces sp. and was named No 248. A production of the antibiotics from No. 248 begins at the early exponential phase developed at the 72th hour under the optinum conditions. The property of No. 248 antimicrobial compound was very stable under acid(pH 3.0) and alkali(pH 10.0) treatment, but it was instable in heat treatment at $120^{\circ}C$. For the improvement of antibiotic activity, two mutants were isolated from strain No. 248 by the treatment of mutagenic agents, NTG and hydroxylamine. As a result, the mutant strains excreted the potent antibiotics to inhibit the growth of Candida albicans.

• Korean Journal of Soil Science and Fertilizer
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• v.10 no.2
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• pp.75-78
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• 1977

Corn (Zea may, L.) was grown alternatively in nutrient solution and hydroxy Al or Al-citrate solution to identify the form of Al which induces Al toxicity on Corn seedlings. Corn seedlings exposed to hydroxy Al solution was very toxic but Al-citrate solution did not show any toxic symptoms. At pH 7 with Al-citrate solution, severe Fe, deficiency was induced probably by the decrease of stability constant of Al-and Fe-oganic complexes and subsequent precipitation of Al-and Fe-as a hydroxide form. Addition of humic acid ameliorated the Al toxicity somewhat at pH 4.7 with hydroxy-Al solution but at pH 7 it induced more severe Fe deficiency.