• Title/Summary/Keyword: Soil bacteria

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Changes of Soil-Emission Gases and Microbial Diversity by Different Fertilizers Supplemented after Application of Livestock-Manure Compost in Greenhouse Soil (시설재배지(施設栽培地)에서 축분퇴비(畜糞堆肥) 시용시(施用時) 보충비종(補充費種)에 따른 토양배출(土壤排出)가스 및 미생물다양성(微生物多樣性)의 변화(變化))

  • Kang, Hang-Won;Ko, Jee-Yeon;Park, Hyang-Mee;Lee, Jae-Saeng;Rang, Ui-Gum;Park, Kyeong-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.1
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    • pp.52-60
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    • 2000
  • This study was conducted to gain basic data for alleviation of gas emission and conservation of healthy soil environment by investigating an aspect of gas emission and microbial diversity due to the supplement of different fertilizers after application with a livestock manure compost in greenhouse soils. Green pepper was cultivated in clay loamy soil from April to August. Before planting, a livestock manure compost was applied with $741mg\;ha^{-1}$ on the basis of the phosphate content contained in compost. And then, deficient nitrogen for cropping was supplemented with either quick-acting fertilizer of urea or a controlled slow release fertilizer made from urea formaldehyde(U/F). $NH_3$ and R $NH_2$ gases emitted from soil showed a low concentration in the early stage but a maximum in 27 days after planting, then decreased rapidly and not detected after 33 days. Their average concentrations were 42% and 85% lower in the treatment of slow release fertilizer than that of urea fertilizer, respectively. $CO_2$ gas emitted under urea fertilization was ranged from 1,200 to $3,200mg{\ell}^{-1}$ and that in slow release fertilizer was $900{\sim}2,650mg\;{\ell}^{-1}$. The average concentration of urea treatment was $2,260mg{\ell}^{-1}$ and 30% higher than that of slow release fertilizer. The treatment of slow release fertilizer with the lapse of cropping time populated larger in numbers of bacteria, actinomycetes, nitrate bacteria and nitrate reduction bacteria, and ratios of bacteria and actinmycetes to fungi than that of urea fertilizer. But the number of fungi was higher in the treatment of urea fertilizer and denitrifying bacteria showed a similar trend in both treatments. The microbial diversity index, which calculated with numbers of 6 species of microorganisms, was decreased with increasing of growing stage in the range of 0.1 to 0.35 and that was higher in the tratment of slow release fertilizer than urea.

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Fermentation of Kanjang, Korean Soy Sauce, in Porosity-Controlled Earthenwares with Changing the Mixing Ratio of Raw Soils (흙배합비를 달리하여 기공율이 조절된 담금용기 항아리에서의 간장 발효)

  • Chung, Sun-Kyung;Lee, Kwang-Soo;Lee, Dong-Sun
    • Korean Journal of Food Science and Technology
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    • v.38 no.2
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    • pp.215-221
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    • 2006
  • This study focuses on investigating the effect of porosity-controlled earthenware on fermentation of kanjang, Korean soy sauce. Porosity of fermentation vessel was controlled by changing the mixing ratio of raw soils at manufacturing earthenware. Earthenwares contented 0%, 40% and 60% of the mixture of red brown soil and powdered soil (1 : 1), respectively. The more contents of the mixed soil, the more porosity in earthenware. During fermentation of kanjang in porosity-controlled earthenwares at $30^{\circ}C$ for 4 months, physical, chemical, microbiological and sensory quality attributes were monitored. Compared to other containers, kanjang in the earthenware that had 0% mixed soil showed less water loss, salt content and pH. It also produced higher total acidity, protease activity, total nucleotide, and microbiological changes which included total aerobic bacteria, lactic acid bacteria and yeast. Total nitrogen and free amino acids in kanjang did not show the consistency with the mixed soil contents of fermentation containers, which may take more effect of other factors as water loss than the porosity of vessels. However, the percentage of glutamic acid in total free amino acids was a little higher in the earthenware that had 0% of mixed soil than other containers. These positive physicochemical, microbiological changes also resulted in higher sensory quality.

Removal Characteristics of NOx Using a Soil-Biofilter (토양 Bio-Filter를 이용한 질소산화물 제거특성)

  • Cho Ki-Chul;Ko Byeung-Ik;Lee Nae-Hyun;Cho Il-Hyoung
    • Journal of Environmental Science International
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    • v.15 no.2
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    • pp.133-139
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    • 2006
  • Soil biofiltration is an environmentally-sound technology for elimination of VOCs, odorous and NOx compounds from a low concentration, high volume waste gas streams because of its simplicity and cost-effectiveness. This study was performed to evaluate effect of removal of gaseous NOx using a soil and a yellow soil. Over $60\%\;and\;48\%$ of NOx from a soil and a yellow soil was removed at the inlet NO concentrations of $423\~451$ppb, respectively. The bio-filter using a soil media was capable of purifying NOx with a different natural processes. Although some of the processes are quite complex, they can broadly be summarized as adsorption into soil pore water, and biochemical transformations by soil bacteria. When the filteration bio-reactor was applied to a soil and a yellow soil, effective NOx removal was obtained for several times and months. These results show that a soil biofilter can be of use as an alternative advanced NOx treatment system.

Studies on the Root Rot of Ginseng(VII) (인삼근부병에 관한 연구. VII)

  • 이민웅
    • Korean Journal of Microbiology
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    • v.15 no.1
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    • pp.20-30
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    • 1977
  • Relationship of soil properties and seasonal variation on microbilogical population to-continuous culture and first-time culture of ginseng was investigated by bimonthly from May 1976 to January 1977. pH and P contents of 2 years continuous culture of soil were higher than other culture plot of soil, and contraty to the above, 2 years first-time culture of ginseng soil was conplot of soil, and contraty to the above, 2 years first-time culture of ginseng soil was contained more potassium contents than other culture plot of soil. In microbiological fluctuation with seasonr in various soil conditions, the population, trends of Fusarium spp., Erwiniaspp., and flourescent Psedudomonas spp. were increased in May and July in general, but decreased in the other month. It was observed that in all type of soil, Fusarium spp. was distributed in abundance in and on rihizosphere, and decreased the propagules numbers as soil depth increase. The numbers of Erwinia spp. and fluorescent Pseudo-monas spp. were distributed greater in numbers on the surface zone of soil depth decreasing the numbers along the soil layer increase, and also in 2years continuous culture of soil especially, a great numbers of Erwinia spp. and fluorescent Pseudomonas were evenly distributed in surface zone and rhizosphere. Ginseng disease with a high incidence of bacterial disease in continuous culture of 2 and 4 years was seemed to be associated with soil bacteria that was high in numbers of Erwinia spp. and fluorescent Pseudomonas spp. in May and July.

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Ecology of Rhizome Rot Incidence of Ginger and Relation of Soil Texture, Chemistry and Biology (생강 근경부패병의 발생 생태 및 토성 , 토양화학성 및 토양 생물성과의 관계)

  • Lee, Wang-Hyu;Lee, Doo-ku
    • Korean Journal of Environmental Agriculture
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    • v.17 no.1
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    • pp.1-4
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    • 1998
  • The ecology of rhizome rot incidence rates between Seosan, Chungnam and Bongdong, Chonbuk province were surveyed in this experiment. The rhizome rot incidence rate of Seosan was lower than that of Bongdong. The average ginger product of former province was 1,140kg, whereas it's 818kg per 10a. in latter province. Treatment after disease incidence frequently used fungicide at Seosan. There were different soil physiological property between fields. Although Eunhari and Yulsori were belong to Bongdong province, incidence rate was low at Eunhari, contained 23% clay and high incidence rate at Yulsori, approximately contained 60% silt. Many soil chemical properties of cultivated area were belong to optimum concentration range, but phosphate(P) was higher than optimum concentration, 450-550ppm. The number of soil fungi in the middle of cultivated season tended to show the highest. Both bacteria and actinomyces` number was similar to the result of fungi. The bacteria/fungi ratio was greatly different between surveyed fields.

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Deproteinized Mulberry Leaf Juice - A New Media for Growth of Microorganisms

  • Chowdary, N.B.;Naik, V.Nishitha;Sharma, D.D.;Govindaiah
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.2
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    • pp.217-220
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    • 2002
  • Mulberry being a foliage crop is grown extensively for feeding of silkworms and are also used for cattle feeding. These loaves are highly nutritious, which contain various mineral elements and bio-molecules such as carbohydrates, proteins, lipids and other essential amino acids, etc. In the present study, deproteinized mulberry leaf juice was used for preparation of the medium for cultivation of various types of microbes. Results revealed that deproteinized mulberry leaf juice medium is best for isolation of fungi, bacteria and actinomycetes and this medium can be substituted with synthetic media, which are haying the costly ingredients for isolation and identification of bacteria, fungi and actinomycetes. Further, this deproteinized mulberry juice medium can also be used in mass multiplication of useful/beneficial microbes to enhance soil microflora to improve soil fertility and to avoid root diseases. Perspective enterprises can take up the mass multiplication/large-scale production of useful microbes such as Trichoderma, Rhizobium, Pseudomonas and Bacillus to use in mulberry and in other agricultural crops using deproteinized mulberry leaf juice.

토양 오염물질의 독성 탐지를 위해 유전자 재조합 발광 박테리아를 이용한 환경 바이오 센서의 개발과 응용

  • Jang, Seok-Tae;Lee, Hyeon-Ju;Gu, Man-Bok
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.212-215
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    • 2000
  • Recombinant bioluminescent bacterial strains that use specific promoters fused to the bioluminescence genes (lux genes) have been applied in environmental monitoring. Advantages of using recombinant bioluminescent bacteria as blosensing cells include rapid responses, low costs, and improved reproducibility. In this study, a recombinant Escherichia coli, GC2, containing a lac::luxCDABE fusion immobilized with solid agar media and glass beads was used to estimate the effect of this soil flushing technique. This bacterium constitutively emits light under normal conditions (no toxic chemicals). When growth and metabolism of these bioluminescent bacteria is inhibited by their exposure to toxic chemicals, the bioluminescence (BL) is reduced. A biosurfactant, rhamnolipids, was used to extract phenanthrene from the soil after flushing.

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퇴적물 내 비소의 지구미생물학적 거동 연구

  • Lee Jong-Un;Lee Sang-U;Park Ji-Min;Kim Gyeong-Ung;Jeon Hyo-Taek;Jeong Myeong-Chae
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2006.04a
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    • pp.111-114
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    • 2006
  • The effects of indigenous bacteria on geochemical behavior of As in As-contaminated sediments (Hwachon mine and Myoungbong mine) after biostimulation with a variety of carbon sources were investigated under anaerobic condition. In Hwachon sediment, As was dramatically extracted from nonsterile sediment with time, reaching the highest concentration of $500{\mu}g/L$. The As leaching was likely caused by microbial dissolution of Fe oxides/oxyhydroxides with which As had been coprecipitated. However, in the case of Myoungbong sediment supplied with glucose, dissolved As decreased with time likely due to production of As sulfide(s) and subsequent precipitation, which resulted from bacterial reduction of $SO_4^{2-}$. The results implied that bacterial in-situ stabilization of As In subsurface has a potential to be practically applied.

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Antagonistic Effect of Chitinolytic Bacteria on Soilborne Plant Pathogens (토양전염성 식물병원균에 대한 Chitin 분해세균들의 길항효과)

  • 박서기;이효연;김기청
    • Korean Journal Plant Pathology
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    • v.11 no.1
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    • pp.47-52
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    • 1995
  • One hundred and thirty bacterial isolates with high chitinolytic activity on chitin agar media were isolated and identified. Most of the isolates were Aeromonas hydrophila (110 isolates), and the others were Serratia marcescens (11 isolates), Aeromonas caviae (3 isolates), Chromobacterium violaceum strain C-61 (2 isolates), Chromobacterium violaceum strain C-72 (1 isolate) and unknown species (3 isolates). Among them, C. violaceum strain C-61 had highest chitinolytic activity and fungal growth inhibition on PDA. This bacterium also inhibited the growth of Rhizoctonia solani, Sclerotinia scelrotiorum, Phytophthora capsici and Pythium ultimum, but it didn't inhibit the growth of Fusarium oxysprum and Fusarium solani. C. violaceum strain C-61 suppressed damping-off of eggplant caused by R. solani. Populations of the chitinolytic bacteria such as Aeromonas hydrophila, Serratia marcescens, Aeromonas caviae, Chromobacterium violaceum strain C-61 and Chromobacterium violaceum strain C-72 introduced into R. solani-infested soil were continuously decreased until 20 days after treatment, but their populations except A. caviae were not changed significantly and maintained over 5$\times$104 CFU per g of soil thereafter.

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Bacteria and Fungi as Alternatives for Remediation of Water Resources Polluting Heavy Metals

  • Joo, Jin-Ho;Hussein, Khalid A.;Hassan, Sedky H.A.
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.4
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    • pp.600-614
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    • 2011
  • Classical methods which used for removal of heavy metals from contaminated water are adsorption, precipitation, coagulation, ion exchange resin, evaporation, and membrane processes. Microbial biosorption can be used for the removal of contaminated waters with pollutants such as heavy metals and dyes which are not easily biodegradable. Microbial biosorbents are inexpensive, eco friendly and more effective for the removal of toxic metals from aqueous solution. In this review, the bacterial and fungal abilities for heavy metals ions removal are emphasized. Environmental factors which affect biosorption process are also discussed. A detailed description for the most common isotherm and kinetic models are presented. This article reviews the achievements and the current status of bacterial and fungal biosorption technology for heavy metals removal and provides insights for further researches.