• Journal of KIISE:Software and Applications
• /
• v.32 no.9
• /
• pp.888-900
• /
• 2005

Several researches have been proposed to formalize the knowledge preconditions problem - j.e., an action or a plan is epistemically feasible. However, since the feasibility is only checked at design-time and is assumed that it will also 1)e feasible at run-time, it is not suitable in the context of Semantic Web services composition, where many agents should share the limited resources required for the execution of Web services composition. Therefore, in this paper, we formalize a transactionally executable Web services composition which enables to guarantee its atomicity. Moreover, in order to formalize the transactional executability, we propose $TL-ALCFK_{NF}$ which extends TL-ALCF with the modal operators K and A. Based on $TL-ALCFK_{NF}$, we show how to carry out the epistemic reasoning with TL-ALCF as a language to represent Semantic Web services composition.

• Journal of Korean Medicine for Obesity Research
• /
• v.18 no.1
• /
• pp.10-18
• /
• 2018

Objectives: Metabolic syndrome is correlated with increased cardiovascular risk and characterized by several factors, including visceral obesity, hypertension, insulin resistance, and dyslipidemia. Several members of a large family of nonselective cation entry channels, e.g., transient receptor potential (TRP) melastatin 7 (TRPM7) channels have been associated with the development of cardiovascular diseases. The purpose of this study was to investigate the effects of Dangkwisoo-san, ginger and curcumin on TRPM7 channel. Methods: Human embryonic kidney (HEK) 293 cells stably transfected with the TRPM7 expression vectors were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, $5{\mu}g/mL$ blasticidin, and 0.4 mg/mL zeocin in a humidified 20% $O_2$/10% $CO_2$ atmosphere at $37^{\circ}C$. Whole-cell patch clamp recordings were obtained using an Axopatch 700B amplifier and pClamp v.10.4 software, and signals were digitalized at 5 kHz using Digidata 1422A. Results: Dangkwisoo-san extract (100, 200, 300, 400, and $500{\mu}g/mL$) inhibited the outward and inward TRPM7 whole-cell currents at dose dependent manner and the half maximal inhibitory concentration $(IC)_{50}$ of Dangkwisoo-san was $218.3{\mu}g/mL$. Also, ginger extract (100, 200, 300, 400, and $500{\mu}g/mL$) inhibited the outward and inward of TRPM7 whole-cell currents in a dose dependent manner and the $IC_{50}$ of ginger was $877.2{\mu}g/mL$. However, curcumin had no effects on TRPM7 whole-cell currents. Conclusions: These results suggest that both Dangkwisoo-san and ginger have good roles to inhibit the TRPM7 channel, suggesting that Dangkwisoo-san and ginger are considered one of the candidate agents for the treatment of metabolic syndrome such as cardiovascular disease.

• Microbiology and Biotechnology Letters
• /
• v.49 no.2
• /
• pp.148-156
• /
• 2021

Single-chain antibodies against epidermal growth factor receptor variant III (EGFRvIII) are potentially promising agents for developing antibody-based cancer treatment strategies. We described in our previous study the successful expression of an anti-EGFRvIII scFv antibody in Escherichia coli. However, we could also observe the formation of insoluble aggregates in the periplasmic space, limiting the production yield of the active product. In the present study, we investigated the mechanisms by which growth conditions could affect the expression of the soluble anti-EGFRvIII scFv antibody in small-scale E. coli NiCo21(DE3) cultures, attempting to maximize production. The secreted scFv molecules were purified using Ni-NTA magnetic beads and protein characterization was performed using SDS-PAGE and western blot analyses. We used the ImageJ software for protein quantification and determined the antigen-binding activity of the scFv antibody against the EGFRvIII protein. Our results showed that the highest percentage of soluble scFv expression could be achieved under culture conditions that combined low IPTG concentration (0.1 mM), low growth temperature (18℃), and large culture dish surface area. We found moderate-yield soluble scFv production in the culture medium after lactose-mediated induction, which was also beneficial for downstream protein processing. These findings were confirmed by conducting western blot analysis, indicating that the soluble, approximately 30-kDa scFv molecule was localized in the periplasm and the extracellular space. Moreover, the antigen-binding assay confirmed the scFv affinity against the EGFRvIII antigen. In conclusion, our study reveals that low-speed protein expression is preferable to obtain more soluble anti-EGFRvIII scFv protein in an E. coli expression system.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.13 no.1
• /
• pp.406-434
• /
• 2019

In general, an information security approach estimates the risk, where the risk is to occur due to an unusual event, and the associated consequences for cloud organization. Information Security and Risk Management (ISRA) practices vary among cloud organizations and disciplines. There are several approaches to compare existing risk management methods for cloud organizations but their scope is limited considering stereo type criteria, rather than developing an agent based task that considers all aspects of the associated risk. It is the lack of considering all existing renowned risk management frameworks, their proper comparison, and agent techniques that motivates this research. This paper proposes Agent Based Information Security Framework for Hybrid Cloud Computing as an all-inclusive method including cloud related methods to review and compare existing different renowned methods for cloud computing risk issues and by adding new tasks from surveyed methods. The concepts of software agent and intelligent agent have been introduced that fetch/collect accurate information used in framework and to develop a decision system that facilitates the organization to take decision against threat agent on the basis of information provided by the security agents. The scope of this research primarily considers risk assessment methods that focus on assets, potential threats, vulnerabilities and their associated measures to calculate consequences. After in-depth comparison of renowned ISRA methods with ABISF, we have found that ISO/IEC 27005:2011 is the most appropriate approach among existing ISRA methods. The proposed framework was implemented using fuzzy inference system based upon fuzzy set theory, and MATLAB(R) fuzzy logic rules were used to test the framework. The fuzzy results confirm that proposed framework could be used for information security in cloud computing environment.

• Journal of Korean Medicine for Obesity Research
• /
• v.18 no.2
• /
• pp.57-63
• /
• 2018

Objectives: Metabolic syndrome is defined by a cluster of major cardiovascular risk factors: obesity, insulin resistance, dyslipidemia, and arterial hypertension. Several members of a large family of nonselective cation entry channels, e.g., transient receptor potential vanilloid 4 (TRPV4) channels have been associated with the development of dyslipidemia and hypertension. The purpose of this study was to investigate the effects of Leejung-tang (Lizhong-tang), Rikkunshito, and Bojungikgi-tang (Buzhongyiqi-tang) on TRPV4 channel. Methods: Human embryonic kidney 293 cells stably transfected with the TRPV4 expression vectors were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, $5{\mu}g/mL$ blasticidin, and 0.4 mg/mL zeocin in a humidified 20% $O_2/10%$ $CO_2$ atmosphere at $37^{\circ}C$. Whole-cell patch clamp recordings were obtained using an Axopatch 700B amplifier and pClamp v.10.4 software (Molecular Devices, San Jose, CA, USA), and signals were digitalized at 5 kHz using Digidata 1422A. Results: Leejung-tang and Rikkunshito (10, 30 and 50 mg/mL) had no effects on the TRPV4 whole-cell currents at dose dependent manner. However, Bojungikgi-tang (10, 30, and 50 mg/mL) inhibited the TRPV4 whole-cell currents in a dose dependent manner and the half maximal inhibitory concentration (IC50) of Bojungikgi-tang was 18.2 mg/mL. Conclusions: These results suggest that Bojungikgi-tang plays an important roles to inhibit the TRPV4 channel, suggesting that Bojungikgi-tang is considered one of the candidate agents for the treatment of metabolic syndrome such as dyslipidemia and hypertension.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.18 no.4
• /
• pp.153-166
• /
• 2008

According to the growth of the internet and the usage of software agents, the CAPTCHA that is a method for taking apart humans and computers has been widely deployed and used. As the results of many research activities, the CAPTCHA, which is spoken for a distorted image material including random text, has known to be easily breakable via artificial intelligence techniques. As one of alternatives for those text-based CAPTCHAs, methods using photos are concerned and various image-based CAPTCHAs are suggested. However, image-based CAPTCHAs still have some problems. In this paper, we discuss what are the problems in each image-based CAPTCHA and propose a new image-based CAPTCHA using image composition as the solution of those problems. Furthermore, for the secure operation of the CAPTCHA, we suggest a communication protocol that works without the virtual session and consider possible security and usability problems in the protocol.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.19 no.6
• /
• pp.105-112
• /
• 2009

Nowadays, completely automated public turing tests to tell computers and humans apart(CAPTCHAs) are widely used to prevent various attacks by automated software agents such as creating accounts, advertising, sending spam mails, and so on. In early CAPTCHAs, the characters were simply distorted, so that users could easily recognize the characters. From that reason, using various techniques such as image processing, artificial intelligence, etc., one could easily break many CAPTCHAs, either. As an alternative, By adding noise to CAPTCHAs and distorting the characters in CAPTCHAs, it made the attacks to CAPTCHA more difficult. Naturally, it also made users more difficult to read the characters in CAPTCHAs. To improve the readability of CAPTCHAs, some CAPTCHAs used different colors for the characters. However, the usage of the different colors gives advantages to the adversary who wants to break CAPTCHAs. In this paper, we suggest a method of increasing the recognition ratio of CAPTCHAs based on colors.

• Journal of Dental Anesthesia and Pain Medicine
• /
• v.21 no.4
• /
• pp.329-336
• /
• 2021

Background: In the field of dentistry, topical anesthetics play an important role in reducing pain during needle pricks. The anesthetic property of betel leaves remain unexplored, even though they have been widely used for the treatment of various ailments. The purpose of this study was to compare and evaluate pain perception following topical application of lignocaine gel, clove gel, ice, and newly developed betel leaf extract gel during intraoral injection in children. Methods: Sixty children aged 6-10 years who met the inclusion criteria were divided into four groups. Topical anesthetic agents, 2% lignocaine (Lox-2% Jelly, Neon, Mumbai, India), 4.7% clove gel (Pain Out Dental Gel, Colgate Palmolive India Ltd, Solan, India), 10% betel leaf extract gel, and ice were applied to each group for one min, followed by administration of infiltration anesthesia. Pain perception was analyzed during needle insertion. The Wong Bakers FACES pain rating scale (WBFPRS) was used for subjective assessment and the Sound, Eye, Motor (SEM) scale for objective assessment. Recorded values were tabulated and subjected to appropriate statistical analysis using SPSS software with a P value set at 0.05. Results: The clove oil and betel leaf groups demonstrated the highest WBFPRS scores, followed by the ice and lignocaine groups. The clove, betel leaf extract, and ice groups showed equal and highest SEM scores, followed by the lignocaine group. The mean WBFPRS and mean SEM scores were statistically significant. Conclusion: Betel leaf extract gel is effective in reducing pain and can act as an alternative topical anesthetic agent.

• Journal of the Korea Society of Computer and Information
• /
• v.27 no.8
• /
• pp.135-142
• /
• 2022

In this paper, we propose a graph-based user route control for rapidly conducting virus inspections in emergency situations (eg, COVID-19) and a framework that can simulate this on a city map. A^* and navigation mesh data structures, which are widely used pathfinding algorithms in virtual environments, are effective when applied to CS(Computer science) problems that control Agents in virtual environments because they guide only a fixed static movement path. However, it is not enough to solve the problem by applying it to the real COVID-19 environment. In particular, there are many situations to consider, such as the actual road traffic situation, the size of the hospital, the number of patients moved, and the patient processing time, rather than using only a short distance to receive a fast virus inspection.

• Restorative Dentistry and Endodontics
• /
• v.47 no.1
• /
• pp.8.1-8.9
• /
• 2022

Objectives: The aim of this study was to compare the color change of the Giomer resin composite (Beautifil-Bulk) by using photographs obtained with a smartphone (iPhone 6S) associated with Adobe Photoshop software (digital method), with the spectrophotometric method (Vita Easyshade) after immersion in different pigment solutions. Materials and Methods: Twenty resin composite samples with a diameter of 15.0 mm and thickness of 1.0 mm were confectioned in A2 color (n = 5). Photographs and initial color readings were performed with a smartphone and spectrophotometer, respectively. Then, samples were randomly divided and subjected to cycles of immersion in distilled water (control), açai, Coke, and tomato sauce, 3 times a day, 20 minutes for 7 days. Later, new photographs and color readings were taken. Results: The analysis (2-way analysis of variance, Holm-Sidak, p < 0.05) demonstrated no statistical difference (p < 0.005) between the methods in all groups. Similar color changes were observed for all pigment solutions when using the spectrophotometric method. For the digital method, all color changes were clinically unacceptable, with distilled water and tomato sauce similar to each other and with statistical differences (p < 0.005) for Coke and açai. Conclusions: Only the tomato sauce produced a color change above the acceptability threshold using both methods of color assessment. The spectrophotometric and digital methods produce different patterns of color change. According to our results, the spectrophotometric method is more recommended in color change assessment.