Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels

당귀수산, 생강, 커큐민의 대사성 질환과 관련된 일과성 수용체 전압 이온통로조절에 관한 연구

  • Kim, Byung Joo (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
  • 김병주 (부산대학교 한의학전문대학원 양생기능의학부)
  • Received : 2018.04.17
  • Accepted : 2018.05.31
  • Published : 2018.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: Metabolic syndrome is correlated with increased cardiovascular risk and characterized by several factors, including visceral obesity, hypertension, insulin resistance, and dyslipidemia. Several members of a large family of nonselective cation entry channels, e.g., transient receptor potential (TRP) melastatin 7 (TRPM7) channels have been associated with the development of cardiovascular diseases. The purpose of this study was to investigate the effects of Dangkwisoo-san, ginger and curcumin on TRPM7 channel. Methods: Human embryonic kidney (HEK) 293 cells stably transfected with the TRPM7 expression vectors were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, $5{\mu}g/mL$ blasticidin, and 0.4 mg/mL zeocin in a humidified 20% $O_2$/10% $CO_2$ atmosphere at $37^{\circ}C$. Whole-cell patch clamp recordings were obtained using an Axopatch 700B amplifier and pClamp v.10.4 software, and signals were digitalized at 5 kHz using Digidata 1422A. Results: Dangkwisoo-san extract (100, 200, 300, 400, and $500{\mu}g/mL$) inhibited the outward and inward TRPM7 whole-cell currents at dose dependent manner and the half maximal inhibitory concentration $(IC)_{50}$ of Dangkwisoo-san was $218.3{\mu}g/mL$. Also, ginger extract (100, 200, 300, 400, and $500{\mu}g/mL$) inhibited the outward and inward of TRPM7 whole-cell currents in a dose dependent manner and the $IC_{50}$ of ginger was $877.2{\mu}g/mL$. However, curcumin had no effects on TRPM7 whole-cell currents. Conclusions: These results suggest that both Dangkwisoo-san and ginger have good roles to inhibit the TRPM7 channel, suggesting that Dangkwisoo-san and ginger are considered one of the candidate agents for the treatment of metabolic syndrome such as cardiovascular disease.

Keywords

References

  1. Cho JY, Ye SY, Kim DH. The Study on Risk Factor of Metabolic Diseases in Pancreatic Steatosis. J Radiol Sci Technol. 2016 ; 39(1) : 81-8. https://doi.org/10.17946/JRST.2016.39.1.10
  2. Kim HG, Han J. Obesity and pancreatic diseases. Korean J Gastroenterol. 2012 ; 59(1) : 35-9. https://doi.org/10.4166/kjg.2012.59.1.35
  3. Ozbulbul NI, Yurdakul M, Tola M. Does the visceral fat tissue show better correlation with the fatty replacement of the pancreas than with BMI?. Eurasian J Med. 2010 ; 42(1) : 24-7. https://doi.org/10.5152/eajm.2010.08
  4. Clapham DE. TRP channels as cellular sensors. Nature 2003 ; 426(6966) : 517-24. https://doi.org/10.1038/nature02196
  5. Lawen A. Apoptosis - an introduction. Bioessays 2003 ; 25 : 888-96. https://doi.org/10.1002/bies.10329
  6. Vannier B, Zhu X, Brown D, Birnbaumer L. The membrane topology of human transient receptor potential 3 as inferred from glycosylation-scanning mutagenesis and epitope immunocytochemistry. J Biol Chem. 1998 ; 273(15) : 8675-9. https://doi.org/10.1074/jbc.273.15.8675
  7. Pedersen SF, Owsianik G, Nilius B. TRP channels: an overview. Cell Calcium. 2005 ; 38(3-4) : 233-52. https://doi.org/10.1016/j.ceca.2005.06.028
  8. Schmitz C, Perraud AL, Johnson CO, Inabe K, Smith MK, Penner R, et al. Regulation of vertebrate cellular Mg2+ homeostasis by TRPM7. Cell. 2003 ; 114(2) : 191-200. https://doi.org/10.1016/S0092-8674(03)00556-7
  9. Aarts M, Iihara K, Wei WL, Xiong ZG, Arundine M, Cerwinski W, et al. A key role for TRPM7 channels in anoxic neuronal death. Cell. 2003; 115(7) : 863-77. https://doi.org/10.1016/S0092-8674(03)01017-1
  10. Elizondo MR, Arduini BL, Paulsen J, MacDonald EL, Sabel JL, Henion PD, et al. Defective skeletogenesis with kidney stone formation in dwarf zebrafish mutant for trpm7. Curr Biol. 2005 ; 15(7) : 667-71. https://doi.org/10.1016/j.cub.2005.02.050
  11. Vriens J, Owsianik G, Voets T, Droogmans G, Nilius B. Invertebrate TRP proteins as functional models for mammalian channels. Pflugers Arch. 2004 ; 449(3) : 213-26.
  12. Sahni J, Scharenberg AM. TRPM7 ion channels are required for sustained phosphoinositide 3-kinase signaling in lymphocytes. Cell Metab. 2008 ; 8(1) : 84-93. https://doi.org/10.1016/j.cmet.2008.06.002
  13. Kim BJ, Lim HH, Yang DK, Jun JY, Chang IY, Park CS, et al. Melastatin-type transient receptor potential channel 7 is required for intestinal pacemaking activity. Gastroenterology. 2005 ; 129(5) : 1504-17. https://doi.org/10.1053/j.gastro.2005.08.016
  14. Kim BJ, Park EJ, Lee JH, Jeon JH, Kim SJ, So I. Suppression of transient receptor potential melastatin 7 channel induces cell death in gastric cancer. Cancer Sci. 2008 ; 99(12) : 2502-9. https://doi.org/10.1111/j.1349-7006.2008.00982.x
  15. Guilbert A, Gautier M, Dhennin-Duthille I, Haren N, Sevestre H, Ouadid-Ahidouch H. Evidence that TRPM7 is required for breast cancer cell proliferation. Am J Physiol Cell Physiol. 2009 ; 297(3) : C493-502. https://doi.org/10.1152/ajpcell.00624.2008
  16. Kim JN, Chae H, Kwon YK, Kim BJ. Effects of schisandra chinensis on human breast cancer cells. Kor J Ori Med Physiol Pathol. 2014; 28(12): 162-8.
  17. Brixel LR, Monteilh-Zoller MK, Ingenbrandt CS, Fleig A, Penner R, Enklaar T, et al. TRPM5 regulates glucose-stimulated insulin secretion. Pflugers Arch. 2010 ; 460(1) : 69-76. https://doi.org/10.1007/s00424-010-0835-z
  18. Touyz RM, He Y, Montezano AC, Yao G, Chubanov V, Gudermann T, et al. Differential regulation of transient receptor potential melastatin 6 and 7 cation channels by ANG II in vascular smooth muscle cells from spontaneously hypertensive rats. Am J Physiol Regul Integr Comp Physiol. 2006 ; 290(1) : R73-8. https://doi.org/10.1152/ajpregu.00515.2005
  19. Zhu Z, Luo Z, Ma S, Liu D. TRP channels and their implications in metabolic diseases. Pflugers Arch. 2011 ; 461(2) : 211-23. https://doi.org/10.1007/s00424-010-0902-5
  20. Sung SK, Kim SJ, Ahn TS, Hong NR, Park HS, Kwon YK, et al. Effects of Dangkwisoo-san, a traditional herbal medicine for treating pain and blood stagnation, on the pacemaker activities of cultured interstitial cells of Cajal. Mol Med Rep. 2015 ; 12(4) : 6370-6. https://doi.org/10.3892/mmr.2015.4203
  21. Wang J, Ke W, Bao R, Hu X, Chen F. Beneficial effects of ginger Zingiber officinale Roscoe on obesity and metabolic syndrome: a review. Ann N Y Acad Sci. 2017 ; 1398(1) : 83-98. https://doi.org/10.1111/nyas.13375
  22. Pan Y, Zhao D, Yu N, An T, Miao J, Mo F, et al. Curcumin improves glycolipid metabolism through regulating peroxisome proliferator activated receptor ${\gamma}$ signalling pathway in high-fat diet-induced obese mice and 3T3-L1 adipocytes. R Soc Open Sci. 2017 ; 4(11) : 170917. https://doi.org/10.1098/rsos.170917
  23. Committee for the Korean Guidelines for the Management of Dyslipidemia. 2015 Korean guidelines for the management of dyslipidemia: executive summary (english translation). Korean Circ J. 2016 ; 46(3) : 275-306. https://doi.org/10.4070/kcj.2016.46.3.275
  24. Liu D, Zhu Z, Tepel M. The role of transient receptor potential channels in metabolic syndrome. Hypertens Res. 2008 ; 31(11) : 1989-95. https://doi.org/10.1291/hypres.31.1989
  25. Nilius B, Owsianik G, Voets T, Peters JA. Transient receptor potential cation channels in disease. Physiol Rev. 2007 ; 87(1) : 165-217. https://doi.org/10.1152/physrev.00021.2006
  26. Inoue R, Jensen LJ, Shi J, Morita H, Nishida M, Honda A, et al. Transient receptor potential channels in cardiovascular function and disease. Circ Res. 2006 ; 99 : 119-31. https://doi.org/10.1161/01.RES.0000233356.10630.8a
  27. Song Y, Hsu YH, Niu T, Manson JE, Buring JE, Liu S. Common genetic variants of the ion channel transient receptor potential membrane melastatin 6 and 7 (TRPM6 and TRPM7), magnesium intake, and risk of type 2 diabetes in women. BMC Med Genet. 2009 ; 10 : 4.
  28. Mori Y, Otabe S, Dina C, Yasuda K, Populaire C, Lecoeur C, et al. Genome-wide search for type 2 diabetes in Japanese affected sib-pairs confirms susceptibility genes on 3q, 15q, and 20q and identifies two new candidate Loci on 7p and 11p. Diabetes. 2002 ; 51(4) : 1247-55. https://doi.org/10.2337/diabetes.51.4.1247
  29. Spiegel DM, Farmer B. Long-term effects of magnesium carbonate on coronary artery calcification and bone mineral density in hemodialysis patients: a pilot study. Hemodial Int. 2009 ; 13(4) : 453-9. https://doi.org/10.1111/j.1542-4758.2009.00364.x
  30. Trzeciakiewicz A, Opolski A, Mazur A. TRPM7: a protein responsible for magnesium homeostasis in a cell. Postepy Hig Med Dosw (Online). 2005 ; 59 : 496-502.
  31. Kozai D, Ogawa N, Mori Y. Redox regulation of transient receptor potential channels. Antioxid Redox Signal. 2014 ; 21(6) : 971-86. https://doi.org/10.1089/ars.2013.5616
  32. Abiria SA, Krapivinsky G, Sah R, Santa-Cruz AG, Chaudhuri D, Zhang J, et al. TRPM7 senses oxidative stress to release Zn2+ from unique intracellular vesicles. Proc Natl Acad Sci U S A. 2017 ; 114(30) : E6079-88. https://doi.org/10.1073/pnas.1707380114
  33. Kim JH, Park SH, Kim YW, Ha JM, Bae SS, Lee GS, et al. The traditional herbal medicine, Dangkwisoo-San, prevents cerebral ischemic injury through nitric oxide-dependent mechanisms. Evid Based Complement Alternat Med. 2011 ; 2011: 718302.
  34. Sellami M, Slimeni O, Pokrywka A, Kuvacic G, D Hayes L, Milic M, et al. Herbal medicine for sports: a review. J Int Soc Sports Nutr. 2018 ; 15 : 14. https://doi.org/10.1186/s12970-018-0218-y
  35. Kim YS, Hong CS, Lee SW, Nam JH, Kim BJ. Effects of ginger and its pungent constituents on transient receptor potential channels. Int J Mol Med. 2016 ; 38(6) : 1905-14. https://doi.org/10.3892/ijmm.2016.2791

Cited by

  1. 최근 10년간 한방비만학회지의 연구동향 분석: 2010-2019년 한방비만학회지 게재논문을 중심으로 vol.20, pp.2, 2018, https://doi.org/10.15429/jkomor.2020.20.2.149