• 제목/요약/키워드: Soft Dead

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Assessment of the resistance of bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum KACC 21701 in Kimchi cabbage genetic resources

  • Parthiban Subramanian;Ho Chul Ko;Seong-Hoon Kim;Jae Eun Lee;Aejin Hwang;Bichsaem Kim;Yoon-Jung Lee;Awraris Derbie Assefa;Onsook Hur;Nayoung Ro;Jung Sook Sung;Ju Hee Rhee;Ho-Sun Lee;Bum-Soo Hahn
    • 환경생물
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    • 제40권4호
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    • pp.433-441
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    • 2022
  • Bacterial phytopathogen Pectobacterium causes soft rot disease in several vegetable crops globally, resulting in heavy agricultural losses at both the pre and postharvest stages. The present work was carried out to screen Kimchi cabbage genetic resources conserved at the National Agrobiodiversity Center, Rural Development Administration, Korea, for resistance against the soft rot pathogen Pectobacterium carotovorum subsp. carotovorum KACC 21701 over a period of three years (from 2020 to 2022). Infection of the phytopathogen was carried out at four-leaf stage and for each accession, twenty-five plants per germplasm were infected with KACC 21701. Kimchi cabbage cultivars Wangmatbaechu, Seoulbaechu, and CR Kiyoshi were used as control. Seven-days post-infection, the Disease Index (DI) values were manually recorded from zero to four, zero matched perfectly heathy plants and four completely dead plants. The 682 accessions of Kimchi cabbage exhibited varying degrees of disease resistance to KACC 21701 and thirty accessions, exhibiting a DI≤2, were considered for replication studies. During the replication studies, four landrace germplasms (IT102883, IT120036, IT120044, and IT120048) and one cultivar(IT187919) were confirmed to be moderately susceptible to KACC 21701. Results of the preliminary screening as well as replication studies were documented for the all the 682 germplasms. Addition of such information to the passport data of stored germplasms might serve as potential bio-resource for future breeders and researchers to develop resistant varieties or study the mechanisms involved in resistance of plants to such phytopathogen.

Use of the Fix and Flap Approach to Complex Open Elbow Injury: The Role of the Free Anterolateral Thigh Flap

  • Chui, Christopher Hoe-Kong;Wong, Chin-Ho;Chew, Winston Y.;Low, Mun-Hon;Tan, Bien-Keem
    • Archives of Plastic Surgery
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    • 제39권2호
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    • pp.130-136
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    • 2012
  • Background : Complex elbow injuries with associated nerve, muscle, or joint injury commonly develop post-inury stiffness. In order to preserve function, joint congruency, elbow stability and durable wound coverage must be achieved in a timely manner. Methods : A retrospective review of patients who underwent orthopaedic fixation followed by free anterolateral thigh (ALT) flap soft tissue coverage was performed. Five patients were identified and included in this study. Results : We present a series of 5 cases managed with this principle. Soft tissue defects ranged in size from $4{\times}9cm$ ($36cm^2$) to $15{\times}30cm$ ($450cm^2$) and were located either posteriorly (n=4) or anteriorly (n=1). Associated injuries included open fractures (n=3) and motor nerve transection (n=2). Wound coverage was achieved in a mean duration of 18.8 days (range, 11 to 42 day). There were no flap failures and no major complications. The mean postoperative active elbow motion was $102^{\circ}$ (range, $45^{\circ}$ to $140^{\circ}$). Conclusions : In our small series we have highlighted the safety and utility of using the free ALT flap in complex elbow injuries. The ALT flap has many advantages which include abundant skin and subcutaneous tissue; vascularised vastus lateralis muscle that was used in our series to obliterate dead space, provide a vascular bed for nerve grafts and combat infection; and, access to fascia lata grafts for reconstruction of the triceps tendon.

Lower Extremity Reconstruction Using Vastus Lateralis Myocutaneous Flap versus Anterolateral Thigh Fasciocutaneous Flap

  • Lee, Min Jae;Yun, In Sik;Rah, Dong Kyun;Lee, Won Jai
    • Archives of Plastic Surgery
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    • 제39권4호
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    • pp.367-375
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    • 2012
  • Background The anterolateral thigh (ALT) perforator flap has become a popular option for treating soft tissue defects of lower extremity reconstruction and can be combined with a segment of the vastus lateralis muscle. We present a comparison of the use of the ALT fasciocutaneous (ALT-FC) and myocutaneous flaps. Methods We retrospectively reviewed patients in whom free-tissue transfer was performed between 2005 and 2011 for the reconstruction of lower extremity soft-tissue defects. Twenty-four patients were divided into two groups: reconstruction using an ALT-FC flap (12 cases) and reconstruction using a vastus lateralis myocutaneous (VL-MC) flap (12 cases). Postoperative complications, functional results, cosmetic results, and donor-site morbidities were studied. Results Complete flap survival was 100% in both groups. A flap complication was noted in one case (marginal dehiscence) of the ALT-FC group, and no complications were noted in the VL-MC group. In both groups, one case of partial skin graft loss occurred at the donor site, and debulking surgeries were needed for two cases. There were no significant differences in the mean scores for either functional or cosmetic outcomes in either group. Conclusions The VL-MC flap is able to fill occasional dead space and has comparable survival rates to ALT-FC with minimal donor-site morbidity. Additionally, the VL-MC flap is easily elevated without myocutaneous perforator injury.

Anterior Cranial Base Reconstruction in Complex Craniomaxillofacial Trauma: An Algorithmic Approach and Single-Surgeon's Experience

  • Shakir, Sameer;Card, Elizabeth B.;Kimia, Rotem;Greives, Matthew R.;Nguyen, Phuong D.
    • Archives of Plastic Surgery
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    • 제49권2호
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    • pp.174-183
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    • 2022
  • Management of traumatic skull base fractures and associated complications pose a unique reconstructive challenge. The goals of skull base reconstruction include structural support for the brain and orbit, separation of the central nervous system from the aerodigestive tract, volume to decrease dead space, and restoration of the three-dimensional appearance of the face and cranium with bone and soft tissues. An open bicoronal approach is the most commonly used technique for craniofacial disassembly of the bifrontal region, with evacuation of intracranial hemorrhage and dural repair performed prior to reconstruction. Depending on the defect size and underlying patient and operative factors, reconstruction may involve bony reconstruction using autografts, allografts, or prosthetics in addition to soft tissue reconstruction using vascularized local or distant tissues. The vast majority of traumatic anterior cranial fossa (ACF) injuries resulting in smaller defects of the cranial base itself can be successfully reconstructed using local pedicled pericranial or galeal flaps. Compared with historical nonvascularized ACF reconstructive options, vascularized reconstruction using pericranial and/or galeal flaps has decreased the rate of cerebrospinal fluid (CSF) leak from 25 to 6.5%. We review the existing literature on this uncommon entity and present our case series of n = 6 patients undergoing traumatic reconstruction of the ACF at an urban Level 1 trauma center from 2016 to 2018. There were no postoperative CSF leaks, mucoceles, episodes of meningitis, or deaths during the study follow-up period. In conclusion, use of pericranial, galeal, and free flaps, as indicated, can provide reliable and durable reconstruction of a wide variety of injuries.

Characteristics and pathogenicity of Cladobotryum mycophilum isolated from cobweb disease of button mushroom (Agaricus bisporus) in Korea

  • Lee, Chan-Jung;Han, Hye-Su;Jhune, Chang-Sung;Cheong, Jong-Chun;Oh, Jin-A;Kong, Won-Sik
    • 한국버섯학회지
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    • 제9권4호
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    • pp.198-201
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    • 2011
  • Cobweb disease symptoms were observed in a mushroom farm in Buye, Korea during a disease survey in 2008-2011. Five isolates of Cladobotryum sp. were obtained from the infected caps and stipes. These isolates of Cladobotryum sp. were identified as C. mycophilum based on their morphological, cultural characteristics and analysis of the ITS sequences. Early symptoms were noticed as round, fleshy, yellowish brown lesions on mushroom caps. Late symptoms progressed when the parasitic fungus formed white cobweb circular colonies on dead or damaged pinheads, spread on the surface of the casing, and covered entirely fruiting bodies. Optimal temperature and pH for mycelial growth on MEA is $23^{\circ}C$ and 6.0. Microscopically the spores of the fungus are large and most 2~3 celled produced on vertically branched conidiophores. Mushroom caps turned dark brown and shrunk due to soft rot. Testing of sensitivity to selected fungicides showed that isolate was highly resistance to Mancozeb and Thiophanate-methyl, moderately sensitivity to Iprodione, and highly sensitivity to Benomyl, Prochloraz-Mn and Carbendazim.

새로운 능동 클램핑방식을 이용한 연료전지용 DC-AC 인버터의 특성 (Characteristic of fuel Cell DC-AC Inverter Using New Active Clamping Method)

  • 김칠용;조만철;문상필;김영준;김홍삼
    • 한국조명전기설비학회:학술대회논문집
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    • 한국조명전기설비학회 2007년도 추계학술대회 논문집
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    • pp.337-340
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    • 2007
  • In the dissertation, a power conversion system for fuel cell is composed of a PWM inverter with LC filter in order to convert fuel cell voltage to a single phase 220[V], In addition, new insulated DC-DC converters are proposed in order that fuel cell voltage is boosted to 380[V]. In this paper, it requires smaller components than existing converters, which makes easy control. The proposed DC-DC converter controls output power by the adjustment of phase-shift width using switch S5 and S6 in the secondary switch, which provides 93-97[%] efficiency in the wide range of output voltage. Fuel cell simulator is implemented to show similar output characteristics to actual fuel cell. Appropriate dead time td enables soft switching to the range where the peak value of excitation current in a high frequency transformer is in accordance with current in the primary circuit. Moreover, appropriate setting to serial inductance La reduces communication loss arisen at light-load generator and serge voltage arisen at a secondary switch and serial diode. Finally, TMS320C31 board and EPLD using PWM switching technique to act a single phase full-bridge inverter which is planed to make alternating current suitable for household.

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KSTAR 토카막 진공용기 및 플라즈마 대향 부품의 탈기체 처리를 위한 가열 해석 (The baking analysis for vacuum vessel and plasma facing components of the KSTAR tokamak)

  • 이강희;임기학;조승연;김종배;우호길
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2000년도 추계학술대회논문집B
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    • pp.247-254
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    • 2000
  • The base pressure of vacuum vessel of the KSTAR (Korea Superconducting Tokamak Advanced Research) Tokamak is to be a ultra high vacuum, $10^{-6}{\sim}10^{-7}Pa$, to produce clean plasma with low impurity containments. For this purpose, the KSTAR vacuum vessel and plasma facing components need to be baked up to at least $250^{\circ}C,\;350^{\circ}C$ respectively, within 24 hours by hot nitrogen gas from a separate baking/cooling line system to remove impurities from the plasma-material interaction surfaces before plasma operation. Here by applying the implicit numerical method to the heat balance equations of the system, overall temperature distributions of the KSTAR vacuum vessel and plasma facing components are obtained during the whole baking process. The model for 2-dimensional baking analysis are segmented into 9 imaginary sectors corresponding to each plasma facing component and has up-down symmetry. Under the resulting combined loads including dead weight, baking gas pressure, vacuum pressure and thermal loads, thermal stresses in the vacuum vessel during bakeout are calculated by using the ANSYS code. It is found that the vacuum vessel and its supports are structurally rigid based on the thermal stress analyses.

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유경성 대망 이식편을 이용한 농흉 치험 3례 (Surgical Treatment of the Postoperative Empyema with Omental Pedicle Flap [OPF] - Report of 3 Cases -)

  • 허용;문준호;안욱수;김병일;이정호;유희성
    • Journal of Chest Surgery
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    • 제25권8호
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    • pp.819-825
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    • 1992
  • The omental pedicle flap[OFF] has been used for management of complicated problems in various fields of cardiovascular surgery. Its unique properties of enhancing neovascularity, relieving lymphedema, providing fibroblasts to promote healing, providing soft tissue coverage, & functioning in the face of existing infection make it ideal in managing many of the more complicated problems facing the thoracic surgeon. We have used omental pedicle for colosing of the bronchial fistula R esophageal fistula with filling the adjoining cavity after pneumonectomy. The successful closure of the bronchial stump with OFF were obtained in 2 cases, but one case was failed who was suffered from the esophageal fistula. The primary operation in each cases were right pleuropneumonctomy for tuberculous empyema in 2 cases & left pneumonectomy for chronic empyema in 1 case, We believe that the OFF is effective for closing fistula due to postoperative empyema k plombage procedures for dead space of infected thoracic cavity.

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우측이부를 포함한 전두피박이환자의 재이식 치험예 (A CASE REPORT OF TOTAL AVULSED SCALP WITH RIGHT TOTAL EAR)

  • 이열희;변기정;김신호
    • 대한치과의사협회지
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    • 제15권2호
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    • pp.115-120
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    • 1977
  • Extensive avulsion of the scalp, or stripping of a large area of soft tissue from the skull, is a serious accidnet. The avulsed scalp is often injured otherwise, such as by crushing, tearing, or from multiple laceration. The surrounding skin may be devitalized. Replacement of the scalp is usually followed by necrosis and sloughing within a short time. In such instances, the outer table of the cranium may become sucessively exposed. dry, dead, and affected by osteomyelitis. Hence the securing of early healing to prevent these is of the utmost importance. The successful case of reimplantation of the completely avulsed scalp, which is exposured to air for about 14 hours, is reported, in which there was partial growth of hair afterwards. The avulsed scalp caused by her long hair being caught in a grain belt was contaminated with hairs & dust. Authors treated this 19-year old female patient by split thickness skin graft, intermediate skin graft, full thickness skin graft from her own avulsed scalp.

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A Dual-Output Integrated LLC Resonant Controller and LED Driver IC with PLL-Based Automatic Duty Control

  • Kim, HongJin;Kim, SoYoung;Lee, Kang-Yoon
    • Journal of Power Electronics
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    • 제12권6호
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    • pp.886-894
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    • 2012
  • This paper presents a secondary-side, dual-mode feedback LLC resonant controller IC with dynamic PWM dimming for LED backlight units. In order to reduce the cost, master and slave outputs can be generated simultaneously with a single LLC resonant core based on dual-mode feedback topologies. Pulse Frequency Modulation (PFM) and Pulse Width Modulation (PWM) schemes are used for the master stage and slave stage, respectively. In order to guarantee the correct dual feedback operation, Phased-Locked Loop (PLL)-based automatic duty control circuit is proposed in this paper. The chip is fabricated using $0.35{\mu}m$ Bipolar-CMOS-DMOS (BCD) technology, and the die size is $2.5mm{\times}2.5mm$. The frequency of the gate driver (GDA/GDB) in the clock generator ranges from 50 to 425 kHz. The current consumption of the LLC resonant controller IC is 40 mA for a 100 kHz operation frequency using a 15 V supply. The duty ratio of the slave stage can be controlled from 40% to 60% independent of the frequency of the master stage.