• 대한의용생체공학회:의공학회지
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• 제37권5호
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• pp.186-194
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• 2016

In this study, a co-axial flow induced microfluidic chip to fabricate pure collagen type I microfiber via the control of collagen type I and Na-alginate gelation process. The pure collagen type I microfiber was generated by selective degradation of Ca-alginate from 'Core-Shell' structured hydrogel microfiber. To make 'Core-Shell' structure, collagen type I solution was introduced into core channel and 1.5% Na-alginate solution was injected into side channel in microfluidic chip. To evaluatethe 'Core-Shell' structure, the red and green fluorescence substances were mixed into collagen type I and Na-alginate solution, respectively. The fluorescence substances were uniformly loaded into each fiber, and the different fluorescence images were dependent on their location. By immoblizing EpH4-Ras and C6 cells within collagen type I and Na-alginate solution, we sucessfully demonstrated the co-culture of EpH4-Ras and C6 cells with 'Core-Shell' like hydrogel microfiber for 5 days. Only to produce pure collagen type I hydrogel fiber, tri-sodium citrate solution was used to dissolve the shell-like Ca-alginate hydrogel fiber from 'Core-Shell' structured hydrogel microfiber, which is an excellent advantage when the fiber is employed in three-dimensional scaffold. This novel method could apply various application in tissue engineering and biomedical engineering.

• 한국식품과학회지
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• 제24권5호
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• pp.482-491
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• 1992

천연 다당류 고무질을 식품에 확대 이용하고자, sodium alginate, gum karaya 및 gum arabic을 가지고 pH 및 농도별로 SPI에 작용시켜 거품특성을 실험하였다. 표면장력은 3종의 고무질을 농도별로 첨가시 전체적으로 SPI의 등전점(pH 4.5) 부근인 pH 4.0 및 5.0에서 각각 $39.9{\sim}44.1$ 및 $41.7{\sim}44.8\;dyne/cm$이었고, pH 7.0 및 8.0에서는 각각 $43.9{\sim}46.4$ 및 $44.6{\sim}46.9\;dyne/cm$로써 전체적으로 대조구보다 다소 높았으며 pH 4.0 및 5.0에서가 pH 7.0 및 8.0에서 보다 다소 낮았다. 비점도는 pH 7.0 및 8.0에서 전체를 통하여 각각 $3.6{\sim}51.8$ 및 $4.0{\sim}51.2$이었으며, 특히 sodium alginate를 농도별로 첨가 시 pH 7.0 및 8.0에서 각각 $11.5{\sim}51.8$ 및 $10.1{\sim}51.2$로써 대조구에 비하여 크게 증가하였다. 거품형성능(overrun)은 15분 whipping시, 고무질의 종류 및 농도에 따라 pH 4.0 및 5.0에서 각각 $965.7{\sim}1689.0$ 및 $1182{\sim}1413%$이었고, pH 7.0 및 8.0에서 각각 $804.7{\sim}1018.0$ 및 $795.0{\sim}962.3%$로써 전체적으로 대조구보다 낮았으며 pH 4.0 및 5.0에서가 pH 7.0 및 8.0에서보다 높았다. 거품안정성(drainage)은 고무질 종류 및 농도에 따라 pH 4.0(대조구, 66.8분) 및 5.0(대조구, 53.4분)에서 각각 $22.7{\sim}41.41 및 $41.5{\sim}83.8$분이었고, pH 7.0(대조구, 20.4분) 및 8.0(대조구, 19.7분)에서 각각 $18.2{\sim}104.7$ 및 $21.0{\sim}84.6$분이었으며, 특히 sodium alginate 첨가시 효과가 현저하였으며 0.2% 첨가시 pH 5.0, 7.0 및 8.0에서 각각 대조구의 57, 413 및 315% 증진되었다. 거품의 열안정성은 15분 whipping시, pH 4.0(대조구, 30.2%) 및 5.0(대조구, 23.7%)에서 각각 $0{\sim}38.0$ 및 $0{\sim}57.0%$이었고 pH 7.0(대조구, 39.6%) 및 8.0(대조구, 43.6%)에서 각각 $0{\sim}59.4$ 및 $36.6{\sim}58.4%$이었으며 sodium alginate 첨가시가 가장 양호하였다. 전체적으로 보아 거품안정성이 높은 것은 열안정성도 높은 경향이며, 표면장력이 낮으면 거품형성능이 높아지고, 비점도가 높으면 거품안정성 및 열안정성이 높아지는 경향이 있었다.

• Macromolecular Research
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• 제12권2호
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• pp.219-224
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• 2004

In this work, we prepared hydrogels for wound dressing from a mixture of poly(vinyl alcohol) (PVA) and alginate using the $\^$60/Co ${\gamma}$-ray irradiation technique. We examined the physical properties of these hydrogels, including gelation, water absorptivity, and gel strength, to evaluate the applicability of these hydrogels for wound dressings. The biocompatibility of these hydrogels was also evaluated in vitro, in cultures of mouse fibroblasts, and in vivo, by subcutaneous implantation studies in rats. The gel content and strength increased upon increasing the radiation dose and upon decreasing the concentration of alginate. The degree of swelling was inversely proportional to the gel content and strength. The degree of cytotoxicity of the ${\gamma}$-ray-treated hydrogels was ca. 60% compared to the (－) control (serum) after 1 day of incubation. When the incubations were prolonged up to 2 days, the toxicity of all the samples decreased remarkably and reached that of the control. Subcutaneous implantation studies in rats indicated that foreign body reactions occurring around the implanted hydrogels were moderate and became minimal upon increasing the implantation time.

• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.418-423
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• 2007

Meats with alginate binders including sodium alginate, glucono-delta-lactone and calcium carbonate were tested in restructured steaks made from M. pectoralis profundus of beef steers in terms of meat quality and binding ability by tumbling time. The treatment with 25 min tumbling time was significantly lower (p<0.05) for crude protein than 5 and 15 min, while higher (p<0.05) for moisture content. This corresponded with sensory panel juiciness ratings, which showed the treatment for longer tumbling times to be significantly juicier (p<0.05) than that for a shorter time. Cooking loss decreased (p<0.05) linearly with an increased tumbling time, and Kramer shear force also significant decreased (p<0.05) with tumbling time. This corresponded with sensory panel tenderness ratings, which showed that the treatment for longer tumbling times was more tender (p<0.05). The texture results indicated that longer tumbling time had lower (p<0.05) hardness and chewiness values. Sensory panels ranked binding ability in the order 5 min, 15 min and 25 min from best to worst, and the overall acceptability for slices from roasts of treatments for 5 and 15 min were rated by the sensory panelists as moderate to very acceptable, but those for 25 min were rated as fair to moderate.

• Journal of Industrial and Engineering Chemistry
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• 제67권
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• pp.326-332
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• 2018

In this study,the result shows that polyvinyl alcohol-sodium alginate (PVA-SA) gel bead crosslinked with sodium sulfate are better among the different methods by comparing the relative mechanical strength, mechanical strength swelling and expansion coefficient of beads in water. Subsequently, anammox biomass entrapment by PVA-SA gel was introduced into continuous stirred tank reactor (CSTR). After 24 operation days, the nitrogen removal efficiency achieved 60%, while the nitrogen loading rate (NLR) was $0.14kgN/m^3/d$ and the experiment data indicated that PVA-SA gel bead crosslinked with sodium sulfate can be used to initiate anammox process. Furthermore, it is an alternative for culturing anammox in a long-term operation.

• KSBB Journal
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• 제14권3호
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• pp.342-350
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• 1999

P. phosphoreum을 이용하여 연속 모니터링 시스템을 개발하고자 free cell과 고정화 세포의 중금속 물질에 대한 반응을 일차로 조사하였다. 고정화 물질로는 절차가 비교적 간단하고 bioluminescence 투과성에 영향을 주지 않는 sodium algmate를 사용하였다. Alginate는 발광미생물의 빛 발생 대사를 저해하지 않을 뿐만 아니라 bioluminescence의 투과성이 탁월하였다. 중금속 물질 중에서 $HgCl_2$, $CdCl_2$, $MnSO_4$, $ZnSO_4$를 선택하여 free cell과 alginate 혼합 세포 및 strontium alginate에 고정화한 세포에 노출시켜 반응을 조사하였으며, 또한 독성 및 strontium alginate에 고정화한 gel을 disc type으로 제조하여 중금속 물질에 대한 bioluminescence의 변화를 조사하였다. Free cell과 disc type의 세포가 alginate 혼합세포 및 strontium alginate로 고정화한 세포에 비해서 비교적 민감한 반응을 보였으며 중금속 물질의 농도에 대하여 bioluminescence intensity의 감소율이 비례하여 나타났다. 특히, 고정화 세포인경우 free cell 보다 중금속의 mass transfer에 미치는 영향 때문에 민감성은 떨어지지만 모두 linear regression curve를 나타내었다. Disc type의 경우 alginate에 혼합한 세포와 strontium alginate로 고정화한 세포보다 민감한 반응을 보인 것은 중금속에 노출된 면적이 넓기 때문으로 사료된다. 독성물질에 대한 반응분석을 위하여 Gamma value로부터 $EC_{50}$을 계산하였으며 이를 이용하여 각 중금속 물질의 농도와 P. phosphoreum의 bioluminescence intensity와의 상관관계 및 각 중금속 물질의 독성정도를 산출하였다. $EC_{50}$값을 이용하여 독성정도를 볼 때 4가지 type 모두 $HgCl_2$가 가장 독성이 강한 것으로 나타났다. 본 연구결과를 종합해 볼때 P. phosphoreum을 고정화 할 경우 bioluminescence에 거의 영향을 미치지 않을 뿐만 아니라 bioluminescence의 안정성에도 기여하기 때문에 모니터링 시스템에 적용할 수 있다. 특히 disc type의 고정화 제재는 free cell과 동등한 민감도를 나타내었기 때문에 빛 안정성을 유지하면서 수질 모니터링을 위한 bioluminescence제재로 이용이 가능하다.

• 한국수산과학회지
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• 제48권5호
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• pp.589-595
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• 2015

Alginates are used to encapsulate various materials, including food, cosmetics, and pharmaceuticals. This study examined the properties and oxidation stability of fish oil capsules manufactured with calcium alginate gels. The fish oil capsules were manufactured by dropping sodium alginate solution and fish oil into a calcium chloride solution through nozzles. The membrane thickness, sphericity, rupture strength and deformation depth of the fish oil capsules were determined. The peroxide value of the fish oil was assayed to determine the oxidation stability of the capsules. The capsules measured approximately 3 mm with a membrane thickness of 90 μm independent of the amount of fish oil added. As the amount of fish oil encapsulated increased, the sphericity, rupture strength and deformation depth of the capsules decreased. The encapsulation efficiency increased until the amount of fish oil was 30%. The oxidation stability of fish oil in capsules was dependent on the type of nozzle, e.g., the oxidation stability of fish oil in capsules made using a double nozzle was greater than with a single nozzle. These results should lead to industrial application of fish oils including eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, as nutraceuticals.

• 한국수산과학회지
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• 제53권5호
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• pp.681-687
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• 2020

Physicochemical properties of fucoidan and alginate extracted from Sargassium horneri were investigated. The alginates were extracted and purified via three different routes (CaCl₂, HCl and ethanol routes), and their rheological properties at various concentrations and temperatures were determined. The yield of fucoidan was 3.08%, and it was mainly composed of fucose and galactose. Fourier-transform infrared spectrum of fucoidan showed strong absorption band at 1,254 and 827 cm^-1 corresponding to the sulfate group. Flow behavior of the alginate solution was characterized using the power-law model. The consistency index increased with increasing concentrations. The sodium alginate solution (1.5%) exhibited Newtonian behavior when extracted via the CaCl₂ and HCl routes, while it exhibited pseudoplastic behavior when extracted via the ethanol route. The effect of temperature on the flow behavior was investigated in terms of the activation energy (E_a), which was obtained using the Arrhenius equation. The E_a value corresponding to the alginate solution decreased with increasing shear rates. The E_a values corresponding to the alginate solutions purified via the CaCl₂, HCl, and ethanol routes were 13.54-18.64 kJ/mol, 13.42-19.21 kJ/mol, and 9.51-10.67 kJ/mol, respectively. The low E_a values corresponding to the solutions extracted via the ethanol route suggest that the flow behavior does not depend significantly on temperature.

• 한국임상수의학회:학술대회논문집
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• 한국임상수의학회 2008년도 추계학술대회
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• pp.178-178
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• 2008

• 멤브레인
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• 제22권6호
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• pp.481-488
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• 2012

폴리아마이드 역삼투막 표면에 음이온 수용성 고분자인 poly(vinyl amine)(PVAm)을 코팅한 후 오염물질인 bovine serum albumin (BSA), humic acid (HA), sodium alginate (SA)에 대하여 파울링 개선효과가 있는지를 알아보고자 하였다. PVAm의 코팅과 파울링 여부는 scanning electron microscopy (SEM)을 통해 관찰하였다. BSA, HA, SA 100 ppm 공급원액을 이용하여 2, 4, 8 bar로 압력을 변화시켜 투과성능실험을 수행한 결과 PVAm으로 코팅되지 않은 막과 코팅된 막 모두 압력증가에 따라 파울링 현상이 심화되었으나 PVAm으로 코팅된 막이 BSA, HA, SA의 경우 모두에서 약 30%이상 투과도가 향상되어 파울링 개선효과가 나타나는 것을 확인할 수 있었다. HA > SA > BSA의 순으로 파울링 개선효과가 나타났으며 HA의 경우 가장 두드러지게 나타났다.