• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.2
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• pp.42-51
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• 2010

Herbs and shrubs are employed for environmental restoration purposes. Among common herbs and shrubs, few species with low germination rates were selected and studied for enhanced germination rates and decreased germination times via soaking treatment. Rhus chinensis, incubator grown samples treated with the bacterial solution for 72hrs followed by immediate seeding showed the highest germination rate of 26.7% and germination period of 5.7 days, 3 days decrease from the control. Treatment of distilled water (t=3.79, p<0.01), nutrient broth (t=4.44, p<0.00) and bacterial solution (t=4.42, p<0.00) showed highly significant difference. In the case of soil tests, treating in the nutrient broth for 72 hrs followed by immediate seeding yielded the the highest germination rate of 23.3% with 7.3 days to initial germination, a decrease of 14.7 days with respect to the control. All the samples followed by immediate seeding showed significant difference (t=2.13, p<0.05). Incubator grown samples of Lespedeza cyrtobotrya showed different results. The incubator samples suspended for 48 hrs in distilled water followed by immediate seeding and 1 day drying displayed the highest germination rate of 96.7%, surpassing that of the control by 33.4%. The incubator samples treated with the distilled water and nutrient broth showed enhanced germination. But only the samples treated with distilled water and nutrient broth for 48hrs showed the increased germination in soil tests. All the sample treated for 24 hrs followed by immediate seeding or dried for 1 day showed initial germination as early as 1 day in incubator. The initial germinations were shortened in the samples treated with distilled water and nutrient broth for 48hrs in soil tests. Lespedeza cuneata incubator sample treated with nutrient broth for 24 hrs and dried for 1 day exhibited the highest germination rate of 83.3%, a 31.1% improvement over the control. The incubator samples treated with distilled water for 48 hrs (t=4.20, p<0.01) showed effective increase of germination. The treatment of distilled water (t=2.96, p<0.05) and bacterial solution (t=2.24, p<0.05) showed significant difference. The germination rates in soil were less than those of incubator and the control. The incubator samples treated with distilled water and bacterial solution displayed 1 day germination period, shortened by 1.3 days compared to the control. For soil grown samples, the samples treated with distilled water showed delayed initial germination and those treated with nutrient broth for 48hrs and bacterial solution for 72hrs shortened initial germination.

• Journal of Korean Ophthalmic Optics Society
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• v.16 no.3
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• pp.237-245
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• 2011

Purpose: The present study was conducted to investigate whether certain repeated physical and/or chemical stimuli added on ophthalmic lenses might induce any changes of the functions of lens coatings. Methods: The changes in lens surface, light transmittance, foggy duration, durability of ophthalmic lenses were determined after the application of tearing-off with tape, rubbing with acetone, soaking in acetone or distilled water of ophthalmic lens (CR-39 material) as physical and/or chemical stimuli. Results: The change of ophthalmic lens surface was detected after soaking in acetone for longer than 30 minutes by observing the lens surface to figure out the functional change of hard coating. The ophthalmic lens soaked in distilled water for 180 minutes showed little functional change of anti-reflection coating as 1% by measuring light transmittance of lens. However, the function of anti-reflection coating was almost disappeared after the ophthalmic lens was soaked in acetone for 60 minutes. The foggy duration of ophthalmic lens soaked in acetone was increased by estimating foggy duration of lens. The lens coating was shown to be defected when the pre-damaged ophthalmic lenses were torn off with tape, rubbed with acetone and soaked in distilled water or acetone by observing pre-damaged lens surface to evaluate its durability. Conclusions: The careful management during ophthalmic lens dispensing or usual eyeglass wearing is needed since the change in ophthalmic lens coatings was shown by repeated physical and/or chemical stimuli.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.5
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• pp.465-472
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• 1994

To improve the germination performance of rice seeds under suboptimal temperature, osmopriming with PEG-6000 was examined. Optimal PEG-6000 concentration to improve germination was 20% PEG-6000 solution, and rice cultivars used in this experiment were Sinunbongbyeo, Gancheokbyeo, Dongjinbyeo. The water content of seeds after soaking for 60 hours in the PEG solution is similar to that of seeds after soaking for 24 hours in the distilled water. Germination performance of the soaked seeds in the PEG solution was higher than that of the soaked seeds in the distilled water or the control, especially under suboptimal temperatures. Electrical conductivity of the soaked seeds in the PEG solution was lower than that of the soaked seeds in the distilled water or the control, and total dehydrogenase activity of the soaked seeds in the PEG solution was higher than that of the soaked seeds in the distilled water or the control. SDS-PAGE results of soluble protein from the embryos of seeds primed differently showed darker band in the seeds soaked in the PEG solution than the seeds soaked in the distilled water or the control at the 68 KD region. Also, band patterns of peroxidase and esterase of embryos soaked in the PEG solution were darked than that of embryos soaked in the distilled water or the control at the Rf 0.94 and Rf 0.87, respectively.

• Korean Journal of Food Science and Technology
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• v.39 no.3
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• pp.289-294
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• 2007

This study was performed to investigate the effects of pre-soaking methods on the preparation of Rehmannia radix Preparata (R.P). The R. radix L (R.L) was soaked in distilled water and traditional Korean wine for 24 hr, then the soaked R.L was treated with a traditional nine-time steaming process. Next, catalpol, 5-hydroxymethyl-2-furaldehyde (5-HMF), polyphenols, flavonoids, antioxidant activities, and ACE inhibition activity were analyzed for the R.P produced by the different methods. The catalpol content of the R.L was 631.4 ppm, but the content decreased as steaming increased to 8-9 times. The 5-HMF, polyphenol, and flavonoid contents of the R.L were 0.12 mg/g, 5.09 mg/g, and 0.83 mg/g, respectively, and these increased gradually with increasing steaming times. As the steaming times of the distilled soaking water increased, the antioxidant activities of 1 mg/mL increased from 19.44% to 75.60% at 14 times of steaming. The ACE inhibition activities of 1 mg/mL of the distilled soaking water increased from 28.70% to 94.78% at 10 times of steaming, but decreased afterward.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.312-312
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• 2017

The study was conducted to select optimal materials for promoting germination rate, high sprouting vigor by priming treatment using PEG 6000 (water potential -0.5 to 2.0 Mpa) and Azolla extracts (0.1, 1.0, and 10.0%) extracted by ethanol, distilled, and/or hot water in colored rice cultivars. Each rice seed (three black rice cultivars and two red rice cultivars) was soaked 24 and 48 hr including untreated control. In black rice, Joseongheugchal rice cultivar, azolla ethanol extract (0.1%) induced highest germination rate, germination speed was taken to 5 days in distilled water and to 3days in Azolla extracts extracted hot water. Otherwise, degree of bacterial inhibition (number of colony, $10^3cfu$) in dry seed, water soaking for 24hr, soaking with fungicide for 24hr and 48hr, soaking with fungicide and aeration for 24hr and 48 hr was 22, 500, 95, and 0.46, respectively. In order to minimize fungal inhibition, a method can be chosen to combination of soaking fungicide and aeration for 48 hr. In seed priming treatments using growth pouch, seed soaking with fungicide did not affect change of germination percentage and germination speed, it delayed only 2 or 4day in the Joseongheugchal rice cultivar. It differs from rice cultivars and priming materials, Azolla extract(0.1 to 1%) promotes seed germination percentage in the Ilpum, Hongjinju, and Joseonghuegchal, in addition, germination in Jeogjinju cultivar was only promoted by PEG solution(10 to 20%), otherwise, it showed much lower or inhibited on the germination in Heugjinju and Sinmyungheugchal rice cultivars. In a paddy field trial, seedling establishment rate by applying PEG6000 and azolla extract did not show significantly statistical difference. When it compared with untreated control, seedling establishment rate was increased over 50% in priming treatments. Interestingly, seedling establishment rate under azolla extract (0.1%) extracted with ethanol was promoted over 2.5 times compared to the control in a black rice, Joseongheugchal and red rice, Jeogjinju.

• Korean Journal of Food Science and Technology
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• v.27 no.5
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• pp.724-728
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• 1995

Chemical compositions of dried Auricularia polytricha mushroom were analyzed when the dried mushroom were soaked in distilled water at different soaking condition. Physicochemical properties of soaked mushroom were investigated. Compositions of chemical components were 12.6% crude protein, 2.1% crude fat, 6.7% crude fiber and 6.2% ash. The contents of Ca, K, Mg were higher than those of other minerals in the sample, but Mn and Ni were of little quantities. The major amino acids of the sample were found to be aspartic and glutamic acid. The increase in the soaking time and temperature increased the ratio of water uptake, amount of water soluble solid, browning and pH value in the soak water. Ribonucleotide contents of the sample after soaking decreased depending on soaking time and temperature.

• KSBB Journal
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• v.23 no.6
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• pp.525-528
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• 2008

In order to compare the germination and growth rate of the sprouts soaked in highly concentrated oxygen water, it with specific amounts of oxygen dissolved was produced in a high pressure reactor by pressuring oxygen. The sprouts were observed after being soaked in $20^{\circ}C$ oxygen water with 20, 30, 40, 50 ppm of oxygen dissolved each. Results of ten days later indicate that the final germination rate of the sprout soaked in 50 ppm oxygen water was $24.6{\sim}28.6%$ higher than that of the sprout soaked in distilled water. The final growth length also measured 6-7 mm higher than the sprout soaked in distilled water, demonstrating that enough supply of oxygen to the sprout induces stability and efficiency in its growth.

• Journal of Applied Biological Chemistry
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• v.57 no.3
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• pp.197-203
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• 2014

Various parameters such as solvent selection, concentration, solid/liquid ratio, soaking time, temperature, stationary, shaking conditions, and repeated extractions were investigated in order to determine the optimum extraction conditions of ${\beta}$-glucosidase from bagasse fermented by mixed culture of Aspergillus niger NRC 7A and Aspergillus oryzae NRRL 447. Among various solvents tested, non ionic detergents gave the best results than the inorganic or organic salt solutions and distilled water. The optimum conditions for extraction of ${\beta}$-glucosidase were 30 min soaking time at $40^{\circ}C$ under shaking condition at 150 rpm, with solid/liquid ratio 1:15 (w/v), which yielded $2882.74{\pm}95.52U/g$ fermented culture (g fc) of enzyme activity. With repeated washes under the above optimum conditions, the results showed that enzyme extracted in the $1^{st}$ and $2^{nd}$ washes represents about 90% of the total activity.

• Forest Science and Technology
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• v.14 no.4
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• pp.160-168
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• 2018

The seeds of Elaeocarpus serratus, a tropical underutilized fruit tree are characterized by hard seed coat and consequent poor water uptake and low germination. To improve the regeneration through seeds, various parameters such as viability of seeds, water uptake, and effect of seed mass on germination and pretreatments were performed using a completely randomized design (CRD). Tetrazolium (TZ) test was conducted using fresh, mature seeds revealed $50{\pm}2.56%$ mean viability. Seeds of different weight classes showed similar pattern of water uptake and the saturation level was achieved at 60 hrs of soaking. Seeds belong to weight class 2.6-3.5g were germinated ($12.5{\pm}1.26%$) with $175{\pm}1.75days$ (d) of mean time taken for germination (MTG). Germination capacity of seeds varied significantly among different populations and Varkala population gave $12.5{\pm}1.1%$ germination with $174.6{\pm}2.5d$ MTG. Among various seed treatments, mechanical scarification was superior in germination and significant reduction in MTG ($p{\leq}0.05$). The mechanical scarification by complete removal of seed coat resulted in $49.2{\pm}1.52%$ germination within a short period of time ($9.52{\pm}0.89d$ MTG). However, the complete removal of seed coat without damaging to embryo is a difficult task. An alternate treatment (Mechanical scarification II) by making cracks on nut faces vertically followed by soaking in distilled water for 24 hrs gave $48.4{\pm}1.73%$ germination with significantly reduced MTG ($12.14{\pm}0.56d$) over unsoaked, untreated control ($6.5{\pm}1.84%$ germination and $197.18{\pm}1.79d$ MTG; $p{\leq}0.05$). This treatment (Mechanical scarification II) is therefore recommended for E. serratus seeds as it can adopt easily and can achieve 7 fold increases in germination over control. The recorded germination through mechanical scarification is in tune with realized viability percentage of the seeds.

• Korean Journal of Food Science and Technology
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• v.27 no.2
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• pp.210-215
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• 1995

Korean medicinal herbs -sasam, gilkyung, jakyak, danggwi, hwangki and chunkung were soaked to the distillate of Korean rice wine for 75 days. The alcohol concentration of distillate, soaking media was adjusted to 45, 35, and 25% respectively with distilled water. Changes in alcohol concentration, pH, optical density, concentration of peoniflorin and decursin were analyzed. Quality of the final product was determined by sensory evaluation. Alcohol concentration was rapidly decreased but pH increased in 15 days and thereafter they showed slow decrease. Decrease of alcohol concentration was affected by the kind of herb and alcohol concentration of soaking media. The strongest effect was observed from danggwi and low alcohol concentration. Concentration of paeoniflorin and decursin, an index component of jakyak and danggwi respectively, showed the similar trend of decrease after increase to maximum concentration. From the sensory evaluation, the best overall quality was obtained from liquors made from 45% alcohol concentration. The quality was decreased in the order of sasam, jakyak, chunkung and hwanggi.