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Genetic Diversity of 14 Indigenous Grey Goose Breeds in China Based on Microsatellite Markers

  • Tu, Yunjie;Chen, K.W.;Zhang, S.J.;Tang, Q.P.;Gao, Y.S.;Yang, N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.1
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    • pp.1-6
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    • 2006
  • This experiment first cloned some microsatellite sequences for goose species by magnetic beads enriched method and studied the genetic structure research of 14 indigenous grey goose breeds using 19 developed and 12 searched microsatellite markers with middle polymorphism. According to the allele frequencies of 31 microsatellite sites, mean heterozygosity (H), polymorphism information content (PIC) and $D_A$ genetic distances were calculated for 31-microsatellite sites. The results showed that 25 of 31microsatellite sites were middle polymorphic, so the 25 microsatellite markers were effective markers for analysis of genetic relationship among goose breeds. The mean heterozygosity was between 0.4985 and 0.6916. The highest was in the Xupu (0.6916), and in the Yan was the lowest (0.4985) which was consistent with that of PIC. The phylogenetic tree was completed through analysis of UPGMA. Fencheng Grey, Shoutou, Yangjiang and Magang were grouped firstly, then Xongguo Grey, Wugang Tong, Changle and Youjiang were the second group; Gang, Yan Xupu and Yili were the third group; Yongkang Grey and Wuzeng were the fourth group. The results could provide basic molecular data for the research on the characteristics of local breeds in the eastern China, and a scientific basis for the conservation and utilization of those breeds.

Study of Hydrazine-Bromine Reaction in Sulfuric Acid Media (히드라진과 브롬의 반응의 메카니즘에 관한연구)

  • Q. Won Choi;Byoung Bin Park
    • Journal of the Korean Chemical Society
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    • v.19 no.6
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    • pp.403-407
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    • 1975
  • The rate constant for the $N_2H_4-Br_2$ reaction has been measured in sulfuric acid media by direct mixing method and chronopotentiometric method. The former gave 105 mole-1 l sec-1 whereas the latter 103 mole-1 l sec-1. It has been inferred that the former represents the rate constant for $N_2H_4-Br_2$ {\rightarrow} $N_2H_2$ and the latter for $N_2H_2+Br_2$ {\rightarrow} X. When the concentrations of KBr and $H_2SO_4$ are both high, drastic reduction of rate constant has been observed.

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Improved Deadbeat Current Controller with a Repetitive-Control-Based Observer for PWM Rectifiers

  • Gao, Jilei;Zheng, Trillion Q.;Lin, Fei
    • Journal of Power Electronics
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    • v.11 no.1
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    • pp.64-73
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    • 2011
  • The stability of PWM rectifiers with a deadbeat current controller is seriously influenced by computation time delays and low-pass filters inserted into the current-sampling circuit. Predictive current control is often adopted to solve this problem. However, grid current predictive precision is affected by many factors such as grid voltage estimated errors, plant model mismatches, dead time and so on. In addition, the predictive current error aggravates the grid current distortion. To improve the grid current predictive precision, an improved deadbeat current controller with a repetitive-control-based observer to predict the grid current is proposed in this paper. The design principle of the proposed observer is given and its stability is discussed. The predictive performance of the observer is also analyzed in the frequency domain. It is shown that the grid predictive error can be decreased with the proposed method in the related bode diagrams. Experimental results show that the proposed method can minimize the current predictive error, improve the current loop robustness and reduce the grid current THD of PWM rectifiers.

Experiments on Single-Disk Pumps for the Transportation of Micro-scale Water Life (미소 수중 생물체 이송용 단판 디스크 펌프의 성능 실험)

  • Zhang, Z.Q.;Chang, S.M.;Jeong, Y.H.;Yang, J.S.
    • The KSFM Journal of Fluid Machinery
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    • v.14 no.6
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    • pp.18-25
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    • 2011
  • A boundary-layer pump with a single disk has been experimented to obtain its characteristic curve by changing the impeller of a centrifugal pump to a single disk. The primary objective to use of these types of pumps is to avoid hurting water life during transportation unnecessarily. The change of impeller should degrade the performance of pump, so we used the method to increase the roughness on the disk with sandpaper and mesh. The enhancement of shear force from the rotation of disk to the internal flow brought an augmentation of momentum transport, and the characteristics were far improved from the original single-disk pump without decreasing the survival rate of water life in the case of Pseudobagrus fulvidraco (bullhead fish). However, in the case of Artemia cyst (zooplankton), the survival rate was very degraded due to the micro scale smaller than turbulent eddy size. The result of this study could be used for the design of transportation and bio-filtering of water lying on a specific bandwidth of its scale of size.

Adverse Effect of the Methanotroph Methylocystis sp. M6 on the Non-Methylotroph Microbacterium sp. NM2

  • Jeong, So-Yeon;Cho, Kyung-Suk;Kim, Tae Gwan
    • Journal of Microbiology and Biotechnology
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    • v.28 no.10
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    • pp.1706-1715
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    • 2018
  • Several non-methylotrophic bacteria have been reported to improve the growth and activity of methanotrophs; however, their interactions remain to be elucidated. We investigated the interaction between Methylocystis sp. M6 and Microbacterium sp. NM2. A batch co-culture experiment showed that NM2 markedly increased the biomass and methane removal of M6. qPCR analysis revealed that NM2 enhanced both the growth and methane-monooxygenase gene expression of M6. A fed-batch experiment showed that co-culture was more efficient in removing methane than M6 alone (28.4 vs. $18.8{\mu}mol{\cdot}l^{-1}{\cdot}d^{-1}$), although the biomass levels were similar. A starvation experiment for 21 days showed that M6 population remained stable while NM2 population decreased by 66% in co-culture, but the results were opposite in pure cultures, indicating that M6 may cross-feed growth substrates from NM2. These results indicate that M6 apparently had no negative effect on NM2 when M6 actively proliferated with methane. Interestingly, a batch experiment involving a dialysis membrane indicates that physical proximity between NM2 and M6 is required for such biomass and methane removal enhancement. Collectively, the observed interaction is beneficial to the methanotroph but adversely affects the non-methylotroph; moreover, it requires physical proximity, suggesting a tight association between methanotrophs and non-methylotrophs in natural environments.

Mixed Integer Linear Programming Model to Determine the Optimal Levels of Technical Attributes in QFD under Multi-Segment Market (다수의 마켓 세그먼트 하에서 품질기능전개 시(時) 기술특성들의 최적 값을 결정하기 위한 혼합정수계획모형)

  • Yang, Jae Young;Yoo, Jaewook
    • Korean Management Science Review
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    • v.33 no.2
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    • pp.75-87
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    • 2016
  • Quality function deployment (QFD) is a widely adopted customer-oriented product development methodology by analyzing customer requirements. It is a main activity in QFD planning process to determine the optimal values of the technical attributes (TAs) so as to achieve the customer requirements (CRs) from the House of Quality (HoQ). In most of the previous research, all the TAs in QFD are assumed to have either continuous or discrete values. In the real world applications, the continuous TAs and the discrete TAs are often mixed in QFD. In this paper, a mixed integer linear programming model is formulated to obtain the optimal values for the continuous TAs and the discrete TAs in QFD planning as well as Branch and Bound (B and B) algorithm is proposed as the solution approach. Finally, the proposed model and solution approach are illustrated with an office chair under multi-segment market, and the sensitivity analysis is performed to study how the proposed model and its solutions respond to the variation for the two elements which are budget and CRs' weights.

Cloning and characterization of ADP-ribosylation factor 1b from the olive flounder Paralichthys olivaceus

  • Son, So-Hee;Jang, Jin-Hyeon;Jo, Hyeon-Kyeong;Chung, Joon-Ki;Lee, Hyung-Ho
    • Fisheries and Aquatic Sciences
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    • v.20 no.6
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    • pp.10.1-10.7
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    • 2017
  • Small GTPases are well known as one of the signal transduction factors of immune systems. The ADP-ribosylation factors (ARFs) can be classified into three groups based on the peptide sequence, protein molecular weight, gene structure, and phylogenetic analysis. ARF1 recruits coat proteins to the Golgi membranes when it is bound to GTP. The class I duplicated ARF gene was cloned and characterized from the olive flounder (Paralichthys olivaceus) for this study. PoARF1b contains the GTP-binding motif and the switch 1 and 2 regions. PoARF1b and PoARF1b mutants were transfected into a Hirame natural embryo cell to determine the distribution of its GDP/GTP-bound state; consequently, it was confirmed that PoARF1b associates with the Golgi body when it is in a GTP-binding form. The results of the qPCR-described PoARF1b were expressed for all of the P. olivaceus tissues. The authors plan to study the gene expression patterns of PoARF1b in terms of immunity challenges.

Clinical and Molecular Genetic Characteristics of Korean Patients with Smith-Lemli-Opitz Syndrome: A Report of New Patients with a Literature Review (한국인 스미스-렘리-오피츠 증후군 환자의 임상 양상 및 유전자형: 새로운 증례 보고 및 문헌 고찰)

  • Ko, Jung Min
    • Journal of The Korean Society of Inherited Metabolic disease
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    • v.14 no.1
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    • pp.48-53
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    • 2014
  • Smith-Lemli-Opitz syndrome (SLO) is a rare, autosomal recessive disease caused by an inborn error in cholesterol synthesis. Patients with this disease suffer from multiple malformations due to reduced activity of 7-dehydrocholesterol reductase (DHCR7), which increases 7-dehydrocholesterol (7DHC) and 8-dehydrocholesterol (8DHC) concentrations and decreases cholesterol concentration in body fluids and tissue. Here, we describe Korean siblings with SLO who were diagnosed recently, and performed a review of literature about Korean cases with SLO to date. Microcephaly and syndactyly of the second and third toes are the most common physical finding in SLOS patients. Other malformations including growth failure, cleft palate or bifid uvula, various heart malformation, genital ambiguity in males are also accompanied. Not all patients showed low levels of serum cholesterol, so DHCR7 mutation analysis can be helpful to confirmative diagnosis. Two mutations on p.R352 locus (p.R352W and p.R352Q) are commonly identified in Korean SLO patients. Although rare in Korea, SLO should be considered in the differential diagnosis of growth failure with intellectual disability, especially in patients with multiple congenital anomalies.

Effects of Small-Group Education for Testicular Self-Examination on Knowledge, Self-efficacy, and Performance Intention in Male College Students (고환자가검진에 대한 소그룹 교육이 남자 대학생의 지식, 자기효능감 및 수행의도에 미치는 효과)

  • Park, So-Mi;Chung, Chae-Weon;Park, Mi-Jeong
    • The Journal of Korean Academic Society of Nursing Education
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    • v.16 no.2
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    • pp.292-300
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    • 2010
  • Purpose: This study examined the effects of Testicular Self-Examination (TSE) small-group education on knowledge, self-efficacy, and performance intention in male college students. Method: A non-equivalent pre-post test of quasi-experimental design was used. Out of 74 male college students selected by convenient sampling, 37 students were assigned to the experimental group and were given TSE small-group education. A 60-minute program consisted of lecture, demonstration, practice, discussion, and Q&A. Result: With an initial homogeneity between the two group, scores of knowledge about testicular cancer (p<.001), self-efficacy for TSE (p<.001), and performance intention of TSE (p<.01) of the experimental group were significantly higher than those of the control group after the intervention. Conclusion: Small group education for TSE was effective in improving knowledge about testicular cancer, self-efficacy for TSE, and intention to perform TSE. Thus, this education program is considered to be useful for testicular cancer prevention and reproductive health promotion in male college students.

Derivation and Analysis of the BER Closed Form in the OFDM Communication System with IQ Imbalance

  • Hieu Nguyen Thanh;Kang Byung-Su;Lee Kwang-Chun;Ryu Heung-Gyoon
    • Journal of electromagnetic engineering and science
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    • v.6 no.3
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    • pp.182-188
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    • 2006
  • Orthogonal frequency division multiplexing(OFDM) is very useful for the wireless communication system. However, OFDM is very sensitive to the radio frequency impairments. One of the most important major impairments is the IQ imbalance between in-phase(l) and quadrature(Q) branches in the up and down-conversion. IQ imbalance can be divided into phase and amplitude imbalances. These imbalances make constellation of signal to expand and rotate. The performance of system is severely degraded. In this paper, a closed-form for the bit error probability of the OFDM signal in IQ imbalance environment is derived in terms of the function of phase and amplitude imbalance parameters. So, it will be convenient and useful to evaluate the performance of OFDM communication system with IQ imbalance. It is confirmed that computer simulation results closely match with the results of the analytical derivation. When phase imbalance $\varphi=20^{\circ}$, amplitude imbalance $\varepsilon=0.1$; 0.3; 0.4; 0.5, BER at $10^{-5}$ is severely degraded by 1.8 dB, 3.12 dB, 4.72, and 8.44 dB, respectively.