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A case of parentage testing in the Thoroughbred horse by microsatellite DNA typing (Microsatellite DNA형에 의한 더러브렛 말의 친자감정예)

  • Cho, Gil-Jae;Yang, Young-Jin;Kim, Bong-Hwan
    • Korean Journal of Veterinary Research
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    • v.43 no.1
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    • pp.25-29
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    • 2003
  • This study was carried out to investigate a usefulness of the microsatellite DNA markers for parentage verification of Thoroughbred (TB) horses. 9 TB horses samples were genotyped for nine international minimum standard markers (AHT4, 5, ASB2, HMS3, 6, 7, HTG4, 10, and VHL20), and the additional panel of four markers, ASB17, CA425, LEX33, and TKY321. This methods consisted of multiplexing PCR procedures, and it showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. Foal I was excluded according to principles of Mendelian genetics in AHT4 (H/K), ASB2 (Q/Q), HMS3 (I/P), HTG4 (M/O), HTG1O (K/R), VHL20 (M/P), ASB17 (F/N), LEX33 (M/O), and TKY321 (G/I) markets. Foal II was excluded with markers AHT5 (K/M), ASB2 (M/N), HMS7 (N/N), HTG1O (K/K), VHL20 (I/I), ASB17 (F/F) and TKY321 (G/I). Foal III was excluded with markers AHT4 (O/O), AHT5 (K/K), ASB2 (M/R), HMS6 (M/P), HMS7 (O/O), HTG10 (R/S), VHL20 (L/M), and ASB17 (N/O). These results suggest that the present DNA typing is so useful for parentage verification of TB horses.

Analyzing the Type of Recognition for College Students' Department Jumpers

  • Cha, Su-Joung
    • Journal of the Korea Society of Computer and Information
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    • v.25 no.7
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    • pp.125-134
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    • 2020
  • In this study, Q methodology was applied to investigate the subjective evaluation of department jumpers and characteristics of each type of recognition for college students. As a result of analyzing, they were classified into three types. Type 1 was recognized that the department jumper plays a role of giving a sense of belonging to the department and promoting the department. In addition, it was analyzed that wearing a department jumper would be cautious, but the activities were comfortable and people around me gave good evaluations and thought it had the effect of enhancing my confidence. In the case of type 2, the department jumper was satisfied because it was an easy-to-work and non-fashionable design, and was always worn when going to university. Type 3 was a type that gives a sense of belonging when wearing the department jumper, and that it was good for the department jumper to follow the fashion. It is thought that a department jumper with a good fit should be developed by reflecting the physical characteristics, showing the image or symbolism, so that the department jumper of college students can function as a uniform.

A Design Methodology of TMN Distributed Object based on Platform Independent Class Repository (플랫폼독립형 클래스저장소에 기반한 TMN 분산객체 디자인 방법론)

  • 이광형;박수현
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.24 no.12B
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    • pp.2233-2248
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    • 1999
  • The TMN that appears to operate the various communication networks generally and efficiently is developed under the different platform environment such as the different hardware and the different operating system. One of the main problems is that all the agents of the TMN system must be duplicated and maintain the software and the data blocks that perform the identical function. Therefore, the standard of the Q3 interface development cannot be defined and the multi-platform cannot be supported in the development of the TMN agent. In order to overcome these problems, the Farming methodology that is based on the Farmer model has been suggested. With the Farming methodology, the software and the data components which are duplicated and stored in each distributed object are saved in the platform independent class repository(PICR) by converting into the format of the independent componentware in the platform, so that the componentwares that are essential for the execution can be loaded and used statically or dynamically from PICR as described in the framework of each distributed object. The distributed TMN agent of the personal communication network is designed and developed by using the Farmer model.

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An Analysis of Factor Attitude on the Induced Abortion before Marriage in Women (미혼여성의 인공유산에 대한 대학생의 인식 및 태도유형)

  • Oh Mi-Jung
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.5 no.2
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    • pp.293-312
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    • 1998
  • This study was designed to find the characteristics and patterns in subjectivity of an attitude on the induced abortion before marriage in women for a university student. Q-methodology was used as a research design and the research procedures were as follows. Q-sampling has been derived from the literature review and interview. Its credibility and validity were also tested by nursing and methodology professors. Total of 34 statements were selected. P-sampling has been drawn and 49 samples were selected. Based on 9 point scale, the slected respondents rated their operant definition on the induced abortion before marriage in women. The results of above procedures were analyzed by PCQ program. The attitude about the induced abortion before marriage in women were analyzed based on the typal array, extreme comments, and the demographic information of study subjects. The results revealed that there were three types of attitude about the induced abortion before marriage in women. The three types were named as follows ; 1) The first type, agree of conservative perspectives, was consisted of 15 subjects. They emphasized the sanctity of human life and the right of life that will never allow the killing fetus and prevention of induced abortion. 2) The second type, agree of liberal perspectives, was consisted of 10 subjects. They insisted the right to choice for women to control their body, so women have appropriate reason, they could choice induced abortion. 3) The third type, agree of compromise perspectives, was consisted of 7 subjects. They emphasized prevention of induced abortion but allowed under social permission. As a result, this study discovered three types of the attitude about the induced abortion before marriage in women. By identifying the nature of each of these types, this study can be useful to develop efficient strategies for preventing induced abortion.

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Molecular diagnosis of spinal muscular atrophy

  • Lee, Ki-Sun;Hwang, Hee-Yu;Lee, Key-Hyoung;Park, Moon-Sung;Hahn, Si-Houn;Hong, Chang-Ho
    • Journal of Genetic Medicine
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    • v.1 no.1
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    • pp.33-37
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    • 1997
  • Spinal muscular atrophy (SMA) is the second most common fatal disease of childhood with autosomal dominant mode of inheritance, and in its less severe form the third most common neuromuscular disease of childhood after Duchenne muscular dystrophy. The genetic defect was found to be on the long arm of chromosome 5 (5q11.2-q13.3) where many genes and microsatellite markers were missing. One of the most important genes is the Survival Motor Neuron (SMN) gene which is homozygously missing in 90% of SMA patients. Another important gene, the Neuronal Apoptosis Inhibitory Protein (NAIP) gene was found to be defective in 67% of SMA type I patients. Studies so far suggest SMA occurs when the genes on the long arm of chromosome 5 are mutated or deleted. Recently our hospital encountered 2 SMA patients of type I and II respectively. These patients both had homozygously defective SMN genes but intact NAIP genes. We are reporting these cases with bibliographic review and discussion. Korean SMA patients presumably have defects in SMN genes similar to those found in European patients, although the significance of NAIP genes remains to be established. SMN gene defects can be easily diagnosed using PCR and restriction enzymes, and this method could be applied towards convenient prenatal diagnosis and towards screening for family members at risk.

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Using Fuzzy Numbers in Quality Function Deployment Optimization (QFD 최적화에서 퍼지 넘버의 이용)

  • Yoo, Jaewook
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.39 no.2
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    • pp.138-149
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    • 2016
  • Quality function deployment (QFD) is a widely adopted customer-oriented product development methodology by translating customer requirements (CRs) into technical attributes (TAs), and subsequently into parts characteristics, process plans, and manufacturing operations. A main activity in QFD planning process is the determination of the target levels of TAs of a product so as to achieve a high level of customer satisfaction using the data or information included in the houses of quality (HoQ). Gathering the information or data for a HoQ may involve various inputs in the form of linguistic data which are inherently vague, or human perception, judgement and evaluation for the information and data. This research focuses on how to deal with this kind of impreciseness in QFD optimization. In this paper, it is assumed as more realistic situation that the values of TAs are taken as discrete, which means each TA has a few alternatives, as well as the customer satisfaction level acquired by each alternative of TAs and related cost are determined based on subjective or imprecise information and/or data. To handle these imprecise information and/or data, an approach using some basic definitions of fuzzy sets and the signed distance method for ranking fuzzy numbers is proposed. An example of a washing machine under two-segment market is provided for illustrating the proposed approach, and in this example, the difference between the optimal solution from the fuzzy model and that from the crisp model is compared as well as the advantage of using the fuzzy model is drawn.

Suggestion of New Rock Classification Method Using the Existing Classification Method (기존의 암반분류법의 조합에 의한 새로운 암반평가법의 제안)

  • SunWoo Choon;Jung Yong-Bok
    • Explosives and Blasting
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    • v.24 no.1
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    • pp.21-28
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    • 2006
  • Rock mass classification systems such as RMR and Q system have been widely served as a simple empirical approach for the design of various rock mass structures in the stage of site survey as well as under the construction. For the RQD determination, the boring is partially carried out and what is more, the survey boring is not normally carried out under construction. Therefore RQD is frequently determined by empirical method or indirect method. Since it is difficult to determine the discontinuity characteristics such as RQD, spacing, persistence, filling and so on, it is essential to develop suitable and simple systems without drilled core and a cert 없 n number of representative parameters. One of the primary objectives of the classification systems for a practicing engineer has been to make it simple to use as a preliminary design tool for the structures in rock mass. In the present study, the modifications for both the RMR and GSI system are suggested by authors to introduce new classification system as well as to improve the scope of some of the existing classification systems for a practicing engineer.

A Round Robin Study of Solid Content Test and Applicability Estimation of FT-IR Analysis for Chemical Admixtures (다자비교시험을 통한 화학혼화제 고형분량 시험법의 신뢰성 및 FT-IR 분석에 대한 효용성 평가)

  • Kim, Jin-Cheol;Yoo, Hyeok-Jin;Kim, Hong-Sam;Park, Ko-Eun
    • Journal of the Korea Concrete Institute
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    • v.27 no.6
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    • pp.695-703
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    • 2015
  • Acceptance criteria for chemical admixtures of cement concrete were investigated in domestic and international specifications. The reliability was verified for solid content test method of chemical admixture examined statistical analysis by round robin test. The applicability of FT-IR spectroscopy for qualitative measurement of multi-compound chemical admixtures verified. From solid content experimental results, outlier analysed using Cochran, Grubbs and Dickson's Q test. Repeatability and reproducibility standard deviation for solid content results showed 0.25 and 0.098% respectively according to KS A ISO 5725-2 procedure, it can be confirmed reliability of test methods. FT-IR spectrum of liquefied or oven-dried chemical admixtures condition showed big differences. It is needed that the FT-IR analysis is performed on dry material. However there's no difference with the applicability of FT-IR spectroscopy for multi-compound chemical admixtures. So the utility of method analysis could not identify.

Efficient Implementations of Index Calculation Methods of Elliptic Curves using Weil's Theorem (Weil 정리를 이용한 효율적인 타원곡선의 위수 계산법의 구현)

  • Kim, Yong-Tae
    • The Journal of the Korea institute of electronic communication sciences
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    • v.11 no.7
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    • pp.693-700
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    • 2016
  • It is important that we can calculate the order of non-supersingular elliptic curves with large prime factors over the finite field GF(q) to guarantee the security of public key cryptosystems based on discrete logarithm problem(DLP). Schoof algorithm, however, which is used to calculate the order of the non-supersingular elliptic curves currently is so complicated that many papers are appeared recently to update the algorithm. To avoid Schoof algorithm, in this paper, we propose an algorithm to calculate orders of elliptic curves over finite composite fields of the forms $GF(2^m)=GF(2^{rs})=GF((2^r)^s)$ using Weil's theorem. Implementing the program based on the proposed algorithm, we find a efficient non-supersingular elliptic curve over the finite composite field $GF(2^5)^{31})$ of the order larger than $10^{40}$ with prime factor larger than $10^{40}$ using the elliptic curve $E(GF(2^5))$ of the order 36.

Adaptive Application of CPP Algorithm to Test Suite Generation for Protocol Conformance Testing (프로토콜 적합성 시험항목 생성시 CPP 알고리즘의 적응적 적용 방안)

  • Kim, Chul
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.12 no.6
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    • pp.597-604
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    • 2019
  • In this paper, we propose an improved method on an adaptive application of the CPP(Chinese Postman Problem) algorithm to the protocol test suite generation for conformance testing. Also, we present an example application of this CPP algorithm to B-ISDN Q.2931 call/connection control procedure for the purpose of showing how it can be adapted to generate a test suite for conformance testing of a communication protocol. The proposed method has an advantage of an optimization technique which finds a minimum cost of test suite from a standardized specification, so this optimization technique of the CPP algorithm can be practically applied to a real environment for testing a conformity of a protocol implementation.