• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7235-7239
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• 2014

Cervical cancer, the third commonest cancer in women worldwide, can be prevented through early detection by cervical screening (Pap smear). The aim of this study was to investigate the attitudes and practice of cervical cancer screening among female undergraduate university students from 25 low, middle income and emerging economy countries. Using anonymous questionnaires, data were collected from 9,194 female undergraduate university students aged 18-26 years (mean age 20.9, SD=2.0) from 26 universities in 25 countries across Asia, Africa and the Americas. Overall, 11.6% of the female students indicated that they had conducted one or more times a cervical (Pap) smear test; 8.3% among 18-20 year-olds and 15.6% among 21-26 year-old students. There was considerable country variation on having had a cervical (Pap) smear test among 21-26 year-old female university students, ranging from 59.2% in Colombia and 50.9% in Barbados to 0% in India and 1.0% in Tunesia. Logistic regression showed that cervical cancer screening importance or positive attitude were highly associated with the cervical screening practice. Moreover, risky sexual behaviour and tobacco use, two cervical cancer risk factors, were associated with screening. Cervical cancer screening practices were found to be inadequate and e fforts should be made to develop programmes that can increase the uptake of cervical cancer screening.

• Journal of Yeungnam Medical Science
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• 제5권2호
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• pp.47-52
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• 1988

1988년 8월초부터 9월말동안 영남의료원 내과를 내원한 사람중 1304명에서 대변도말검사를 실시하였으며, 이중 443명에서는 간흡충증 피내반응검사를 하였고, 간흡충증이 의심되는 79명에 대해서는 집란법을 동시에 시행하여 간흡충증을 조사한 결과 다음과 같은 결과를 얻었다. 1) 간흡충 감염율은 3.8%였다. 2) 간흡충증 피내반응검사의 감수성은 82.1%(32/39명)이었고, 특이성은 64.6%(261/404명)이었다. 3) 도말검사상 음성이었으나 피내반응검사가 양성이면서 간흡충증이 의심되거나, 양 검사 모두 음성이나 말초 호산구성 세포의 증가로 간흡충증이 의심되는 사람 79명을 집란법으로 조사한 결과 79명중 16명에서 간흡충란이 발견되었다. 4) 도말검사상 음성이었으나 집란법으로 충란이 발견된 16명중 13명은 경감염이었고 3명은 중등감염된 것으로 나타났다. 이상의 결과에서 간흡충증이 의심되는 환자에게는 반드시 대변도말검사와 함께 피내반응검사를 실시하고 필요시 집란법으로 EPG와 EPD을 조사한 후 확진하여 치료하는 것이 바람직하리라 생각된다.

• 대한후두음성언어의학회지
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• 제19권1호
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• pp.43-46
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• 2008

Background and Objectives : Clinical suspicion and appropriate diagnostic procedures are essential for the timely management of extrapulmonary type of mycobacterial disease. In the hope of suggesting a suitable guideline for the early diagnosis of laryngeal tuberculosis, the authors reviewed their clinical pathways and the characteristics of patients with laryngeal tuberculosis who were managed in the recent 10 years at a single tertiary referral hospital, Samsung Medical Center. Subjects and Method : Retrospective chart review was performed for the 25 adult patients with laryngeal tuberculosis. Among 25 cases, 12 were pathologically confirmed by laryngeal biopsy and the other 13 were clinically diagnosed by cumulative clinical information; definite laryngitis on laryngoscopy, positive AFB (acid fast bacillus) smear/culture or active pulmonary tuberculosis on chest X-ray, and substantial response to anti-tuberculosis medication. Results : Chest X-ray revealed active pulmonary tuberculosis in 72% of patients (N=18/25). Sputum AFB smear/culture was positive in 95% of all tested patients (N=21/22) and in 100% of the tested patients who have stable or no evidence of pulmonary tuberculosis (N=5/5). All patients except one who had coexisting laryngeal malignancy showed considerable improvement in their subjective symptoms and laryngeal findings within the first 2 months of anti-tuberculosis medications and they achieved complete response on subsequent sputum studies, chest X-ray and laryngeal findings after $7.0{\pm}2.3$ months of the medications. Conclusion : We suggest that chest X-ray and sputum AFB smear/culture to be the first step of work-up for the patients having laryngeal tuberculosis in suspicion since laryngeal tuberculosis is largely associated with active pulmonary tuberculosis and/or sputum AFB study offers high yield even in case of primary laryngeal tuberculosis. However laryngeal biopsy must be considered in case showing unsatisfactory response to the anti-tuberculosis medication for more than 2 months.

• 복식문화연구
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• 제11권3호
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• pp.441-446
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• 2003

Water-insoluble chitosan with molecular weight of 2,000,000, 500,000, 80,000, and 40,000 and more than 90% of degree of deacetylation were used to test antibacterial activity of chitosan against a pathogenic bacteria, methicillin resistant Staphylococcus aureus(MRSA), which is being issued in the world. As experimental method, Agar plate Smear Method and Agar plate Contact Method were used. The moleculur weight of chitosan didn't exert significant influences on its antibacterial activity against MRSA but chitosan having molecular weight 40,000, 80,000 and 150,000 showed the excellent antibacterial activity. The antibacterial efHciency was excellent in applying it after chitosan was dissolved in acetic acid solution, while the antibacterial efficiency was not expressed nearly in case of applying after chitosan was dissolved in neutral water. Therefore, it is considered that chitosan can show the antibacterial efficiency only if a positive ion status of -NH₃/sup +/ is maintained. MIC of chitosan/acetic acid solution and cotton fabrics finished with chitosan/acetic acid solution showed in concentration of 0.05%.

• Parasites, Hosts and Diseases
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• 제21권1호
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• pp.102-104
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• 1983

A total of 2,026 leprosy patients of the National Sorokdo Hospital was examined their infestparasites by cellophane thick smear method in January, 1983. The egg positive cases of Taenia spp. were treated with bithionol and the segments of Taenia were collected for species identification. The results were as follows: 1. Total egg Positive rate of any kind helminth was 78.2% and cumulative total was 85.2%. The egg positive rate for each helminth was as follow; Taenia spp. 3.4%, Ascaris lumbricoides 4.5%, Trichuris trichiura 72.1%, Clonorchis sinensis 2.8% and other 0.05%. 2. A total of 66 Taenia eggpPositive cases was treated; out of them proglottids of Taenia were collected from 26 cases. All of the collected worms were identified as T. saginata. The results revealed signiBicantly high egg positive rate of T. trichiura. However, A. lumbricoides was found to be controlled considerably by repeated chemotherapy during past 3 years. If chromotherapeutic agent is replaced with oxantel-pyrantel tablet, better result is expected. No clue was found for prevalence of T. sodium from both human and the pig in the island.

• Tuberculosis and Respiratory Diseases
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• 제72권5호
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• pp.409-415
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• 2012

Background: The isolation of non-tuberculous mycobacteria (NTM) has been increasing in South Korea. To date, however, the cause of this increase has not been determined, and it remains unclear whether the use of liquid media has contributed to this increase. The aim of this study was to evaluate the factors associated with NTM isolation and the impact of liquid media on NTM culture. Methods: Mycobacterial smear/culture results of respiratory specimens (sputum and bronchial aspirates), obtained during the years 2002, 2005, and 2010, were retrieved and analyzed retrospectively. Results: During the years 2002, 2005, and 2010, 83,096 sputum specimens were collected from 31,104 patients, and were cultured for mycobacteria, using solid media only in the 2002 and 2005 specimens and both solid and liquid media in the 2010. Of these, 3,516 (4.2%) specimens were smear-positive for acid-fast bacilli (AFB). The annual rate of NTM among positive culture specimens increased from 21% in 2002 to 57.8% in 2010 (p<0.001), as did the proportion of NTM, among AFB smear- and culture-positive specimens, from 12.2% in 2002 to 45.2% in 2010 (p<0.001). In 2010, the NTM culture rate was higher in the liquid than in the solid media (13.9% vs. 8.4%, p<0.001). The NTM rate among AFB-positive specimens was higher in patients aged >50 than ${\leq}$50 years. Conclusion: The rate of NTM isolation has steadily been increasing at the hospital in South Korea, likely due in part to the use of liquid media for the culture.

• 대한세포병리학회지
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• 제17권1호
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• pp.38-45
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• 2006

Bile duct brush cytology has been employed as a diagnostic tool for the evaluation of pancreatic and biliary tract strictures. The specificity of this method is high however, its sensitivity is quite low. A recent study employing liquid based cytology (LBC) reported results comparable to those achieved via conventional cytology. Therefore, we have attempted to prospectively evaluate the diagnostic utility of bile duct brush cytology in pancreaticobiliary diseases. A total of 46 cases with bile duct stricture were enrolled including 11 cases of benign stricture, 29 cases of bile duct carcinoma, 3 cases of gallbladder cancer, and 3 cases of pancreatic cancer. Both conventional smear and LBC using $MonoPrep2^{TM}$ system were conducted in each case. The cytological diagnosis of each case was classed into the following categories; benign, suspicious for malignancy, and malignancy. The diagnostic accuracy of both cytologic methods was investigated. LBC evidenced a high rate of material insufficiency (13/46), which was attributed to low cellularity. The kappa index of both cytological methods was 0.508. Cytological and tissue diagnoses were correlated in 25 cases conducted from biopsy or operation. The sensitivity, specificity, positive predictive value, and negative predictive value were 41.2% (7/17), 100% (8/8), 100% (7/7), and 44.4% (10/18) in conventional smear; 58.8% (10/17), 87.5% (7/8), 90.9% (10/11), and 50.0% (7/14) in LBC; and 94.1% (16/17), 87.5% (7/8), 94.1% (16/17), and 87.5% (7/8) in any one of both cytological methods, respectively. Based on these results, the sensitivity of LBC was found to be superior to that of conventional smear and we were able to obtain higher positive predictive value upto 94.1% by simultaneously conducting both cytologic methods.

• Tuberculosis and Respiratory Diseases
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• 제53권6호
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• pp.635-643
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• 2002

연구배경 : 완전 자동화된 액체배양법과 기존의 고체배양법을 이용하여 폐결핵환자의 객담내 mycobacterium의 신속검출율 및 양성판정시까지의 시간을 비교하고자 하였다. 방 법 : 2002년 1월 1일부터 2002년 6월 30일 사이에 본원에 입원 혹은 외래에서 폐결핵으로 치료중인 환자 127명의 객담검체를 이용하였으며, 배양 중 오염된 28개의 검체(MB/BacT system 18개, L-J배지 14개, 4검체는 두 배양법 모두에서 오염)를 제외한 99명의 객담검체를 대상으로 하였다. 검체는 4% NaOH로 전처치한 후 4300rpm으로 20분간 원심분리한 후 멸균된 phosphate buffer(pH 6.8)를 첨가하여 2ml로 만들어 MB/BacT bottle에 0.5ml, Lowenstein-Jensen배지에 0.25ml를 접종한 후 $35-37^{\circ}C$에 6-12주간 배양하면서 균검출율과 양성판정시까지의 시간을 비교하였다. 결 과 : MB/Bact system과 L-J배양볍 중 어느 한 방법에서 균성장이 발견된 검제는 677B (67.7%) 였다. 두배양법 모두에서 균성장이 발견된 겸체는 5252.5%) 개였으며, MB/BacT에서만 발견된 검체는 15개 (15.2%) 였으나 L-J배양법에서만 발견된 경우는 없었다. 객담검체의 ZN염색법으로 항산균 집균도말겸사상 양성인 검체는 58개, 음성인 검체는 41개였다. 58개의 도말양성 검체 가운데 MB/BacT system에 양성을 보인 검체는 %개 (96.6%), L-J배지양성은 467B (79.3%) 였으며, 균성장 평균 발견시간은 각각 11.0일과 23.5일이었다. 41개의 도말음성검체 가운데 MB/BacT system에 양성을 보인 검체는 11개 (26.8%), L-J배지양성은 6개 (14.6%)였으며, 균성장 평균 발견시간은 각각 13.7일과 27.6일 이었다. 전체적으로 MB/BacT system과 L-J배양법에서 균성장 발견시 간은 각각 $13.3{\pm}8.3$일, $27.2{\pm}0.9$일이었다. 결 론 : 완전 자동화된 MB/BacT system은 L-J배지에 비해 mycobacterium의 검출율이 높고 발견시간이 단축되었다. MB/BacT는 방사성 물질을 사용하지 않는 장점이 있으므로 모든 검사실에 사용하기에 적당할 것으로 보이며, 균동정을 위하여 특이한 DNA probe와 병용하면 CDC가 권고한 결과보고 시간에 근접할 수 있을 것으로 생각되었다.

• Tuberculosis and Respiratory Diseases
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• 제45권3호
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• pp.519-528
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• 1998

연구배경: PCR이 지닌 고유의 문제점으로 인해 PCR의 유용성이 평가절하되기도 하였으나 임상적인 맥락에서 PCR을 환자의 진단과 치료에 이용함에 있어 그 유용성을 평가하는 것이 매우 중요하다고 생각된다. 따라서 저자들은 객담 항산균검사로 폐결핵의 진단이 어려울 때 기관지폐포세척액(Bronchoalveolar lavage fluid, BALF) 에 대한 nested PCR을 시행하여 결핵의 진단에서의 유용성을 알아보고자 하였다. 방 법: 단순 흉부사진상 활동성 폐결핵이 의심되나 향산균이 검출되지 않는 환자 혹은 객담의 배출이 불가능한 환자를 대상으로 기관지폐포세척술(Bronchoalveolar lavage, BAL)을 시행하여 세척액으로 결핵균 도말과 배양, PCR 검사를 시행하고 그 결과를 비교하였다. 결 과: BALF 도말 검사 민감도는 20% 배양 검사 민감도는 38%, BALF PCR의 민감도는 40%, 특이도는 95%였다. BALF에서 배양 양성일 때 PCR이 양성인 경우는 47%였고 BALF 배양 음성시 PCR 양성인 경우는 36%였다. BALF PCR 양성일 때 결핵에 대한 예측치는 89%였다. 기관지내시경을 이용한 결핵균 도말 및 PCR 검사로 40례중 18례(45%)에서 조기에 추가 진단이 가능하였으며 이들 중 8례는 도말로 진단이 되었고 10례 (25%) 는 BALF PCR로만 진단이 가능하였다. 객담 검사가 PCR까지 포함하여 모두 음성일 때 BALF 도말로 진단한 경우가 29명중 5명(17%)이었고 도말도 음성으로 나와 PCR로 진단한 경우가 5명(17%)이였다. 배양검사는 2개월이 지나야 결과를 알 수 있으므로 BAL을 시행하여 조기 진단이 된 경우는 29명중 10명이 된다. BAL PCR로만 진단된 경우는 5명으로 12.5%에서 진단이 가능하였다. 결 론: PCR은 과거와는 달리 검사비용이 비교적 저렴한 검사로 결핵의 진단율을 높이고 수일내에 빠른 결과를 얻을 수 있을 것으로 기대된다. BALF PCR 검사를 시행하는 것이 비용과 이득을 따져볼 때 반드시 시행해야 하는 검사라고 결론을 내리기는 어려우나 임상적 상황에 따라 적절히 이용된다면 결핵의 진단율을 높이고 수시간 내에 빠른 결과를 얻을 수 있을 것으로 기대되며, 면역억제 환자의 경우는 치료가 늦어지면 생명이 위험한 경우가 많아 특히 PCR이 유용한 검사가 될 것으로 생각된다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.7381-7384
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• 2015

This study was conducted to 1206 women who had cervical cancer screening at Chonburi Cancer Hospital. The spilt-sample study aimed to compare the efficacy of abnormal cervical cells detection between liquid-based cytology (LBC) and conventional cytology (CC). The collection of cervical cells was performed by broom and directly smeared on a glass slide for CC then the rest of specimen was prepared for LBC. All slides were evaluated and classified by The Bethesda System. The results of the two cytological tests were compared to the gold standard. The LBC smear significantly decreased inflammatory cell and thick smear on slides. These two techniques were not difference in detection rate of abnormal cytology and had high cytological diagnostic agreement of 95.7%. The histologic diagnosis of cervical tissue was used as the gold standard in 103 cases. Sensitivity, specificity, positive predictive value, negative predictive value, false positive, false negative and accuracy of LBC at ASC-US cut off were 81.4, 75.0, 70.0, 84.9, 25.0, 18.6 and 77.7%, respectively. CC had higher false positive and false negative than LBC. LBC had shown higher sensitivity, specificity, PPV, NPV and accuracy than CC but no statistical significance. In conclusion, LBC method can improve specimen quality, more sensitive, specific and accurate at ASC-US cut off and as effective as CC in detecting cervical epithelial cell abnormalities.