• Molecules and Cells
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• v.40 no.8
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• pp.587-597
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• 2017

MicroRNAs (miRNAs) are essential small RNA molecules that regulate the expression of target mRNAs in plants and animals. Here, we aimed to identify miRNAs and their putative targets in Hibiscus syriacus, the national flower of South Korea. We employed high-throughput sequencing of small RNAs obtained from four different tissues (i.e., leaf, root, flower, and ovary) and identified 33 conserved and 30 novel miRNA families, many of which showed differential tissuespecific expressions. In addition, we computationally predicted novel targets of miRNAs and validated some of them using 5' rapid amplification of cDNA ends analysis. One of the validated novel targets of miR477 was a terpene synthase, the primary gene involved in the formation of disease-resistant terpene metabolites such as sterols and phytoalexins. In addition, a predicted target of conserved miRNAs, miR396, is SHORT VEGETATIVE PHASE, which is involved in flower initiation and is duplicated in H. syriacus. Collectively, this study provides the first reliable draft of the H. syriacus miRNA transcriptome that should constitute a basis for understanding the biological roles of miRNAs in H. syriacus.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.38 no.4
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• pp.72-79
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• 2015

A number of plating companies have been exposed to the risk of fire due to unexpected temperature increasing of water in a plating bath. Since the companies are not able to forecast the unexpected temperature increasing of water and most of raw materials in the plating process have low ignition temperature, it is easy to be exposed to the risk of fire. Thus, the companies have to notice the changes immediately to prevent the risk of fire from plating process. Due to this reason, an agile and systematic temperature monitoring system is required for the plating companies. Unfortunately, in case of small size companies, it is hard to purchase a systematic solution and be offered consulting from one of the risk management consulting companies due to an expensive cost. In addition, most of the companies have insufficient research and development (R&D) experts to autonomously develop the risk management solution. In this article, we developed a real time remote temperature monitoring system which is easy to operate with a lower cost. The system is constructed by using Raspberry Pi single board computer and Android application to release an economic issue for the small sized plating manufacturing companies. The derived system is able to monitor the temperature continuously with tracking the temperature in the batch in a short time and transmit a push-alarm to a target-device located in a remoted area when the temperature exceeds a certain hazardous-temperature level. Therefore, the target small plating company achieves a risk management system with a small cost.

• Journal of Animal Science and Technology
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• v.63 no.4
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• pp.854-863
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• 2021

Weaning induces physiological changes in intestinal development that affect pigs' growth performance and susceptibility to disease. As a posttranscriptional regulator, microRNAs (miRNAs) regulate cellular homeostasis during intestinal development. We performed small RNA expression profiling in the small intestine of piglets before weaning (BW), 1 week after weaning (1W), and 2 weeks after weaning (2W) to identify weaning-associated differentially expressed miRNAs. We identified 38 differentially expressed miRNAs with varying expression levels among BW, 1W, and 2W. Then, we classified expression patterns of the identified miRNAs into four types. ssc-miR-196a and ssc-miR-451 represent pattern 1, which had an increased expression at 1W and a decreased expression at 2W. ssc-miR-499-5p represents pattern 2, which had an increased expression at 1W and a stable expression at 2W. ssc-miR-7135-3p and ssc-miR-144 represent pattern 3, which had a stable expression at 1W and a decreased expression at 2W. Eleven miRNAs (ssc-miR-542-3p, ssc-miR-214, ssc-miR-758, ssc-miR-4331, ssc-miR-105-1, ssc-miR-1285, ssc-miR-10a-5p, ssc-miR-4332, ssc-miR-503, ssc-miR-6782-3p, and ssc-miR-424-5p) represent pattern 4, which had a decreased expression at 1W and a stable expression at 2W. Moreover, we identified 133 candidate targets for miR-196a using a target prediction database. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the target genes were associated with 19 biological processes, 4 cellular components, 8 molecular functions, and 7 KEGG pathways, including anterior/posterior pattern specification as well as the cancer, PI3K-Akt, MAPK, GnRH, and neurotrophin signaling pathways. These findings suggest that miRNAs regulate the development of the small intestine during the weaning process in piglets by anterior/posterior pattern specification as well as the cancer, PI3K-Akt, MAPK, GnRH, and neurotrophin signaling pathways.

• Journal of Ocean Engineering and Technology
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• v.33 no.3
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• pp.289-299
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• 2019

Various sizes of ROVs are being utilized in offshore industrial, scientific, and military applications all around the world. Because of innovative developments in science and technology, image acquisition devices such as sonar devices and cameras have been reduced in size and their performance has been improved. Thus, we can expect better accuracy and higher resolution even in the case of exploration using a small ROV. The purpose of this paper is to prepare a standard procedure for the identification of unidentified hazardous materials found during the National Oceanographic Survey. In this paper, we propose an IDEF (Integrated DEFinition) method modeling technique to identify unidentified targets using a small ROV. In accordance with the proposed procedure, an ROV survey was carried out on target No.16 with a four-ton-class fishing boat as a support vessel on September 18th of 2018 in the sea near Daebu Island. Unidentified targets, which were not known by the multi-beam data obtained from the ship, could be identified as concrete pipes by analyzing the HD camera and high-resolution sonar images acquired by the ROV. The whole proposed procedure could be verified, and the survey with the small ROV required about 10 days to identify the target in one place.

• Geophysics and Geophysical Exploration
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• v.9 no.4
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• pp.299-303
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• 2006

Geologic noise, especially located at shallow depth, can be a great obstacle in the interpretation of geophysical data. Thus, it is important to suppress geologic noise in order to accurately detect major anomalous bodies in the survey area. In the inversion of geophysical data, model parameters at shallow depth, which have small size and low contrast of physical property, can be regarded as one of geologic noise. The least-squares method with smoothness constraint has been widely used in the inversion of geophysical data. The method imposes a big penalty on the large model parameter, while a small penalty on the small model parameter. Therefore, it is not easy to suppress small anomalous boies. In this study, we developed a new inversion scheme which can effectively suppress geologic noise by imposing a big penalty on the slowly varying model parameter and a small penalty on the largely varying model parameter. We call the method MTE (main-target emphasizing) inversion. Applying the method to the inversion of 2.5D small loop EM data, we can ensure that it is effective in suppressing small anomalous boies and emphasizing major anomalous bodies in the survey area.

• Journal of the Korea Safety Management & Science
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• v.15 no.1
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• pp.293-301
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• 2013

The purpose of this study is to introduce the limit of previously used six sigma quality process evaluation metrics, $Z_{st}$ and $P_{pk}$, and a solution to overcome this drawback by using a metric based on performance evaluation of Z-factor quality innovation. Case analysis on projects from national six sigma contest from 2011 to 2012 is performed and literature review on new drug development HTS (High Throughput Screening) is used to propose innovative performance evaluation metrics. This research shows that experimental study on six sigma evaluation metric, $Z_{st}$ and $P_{pk}$, have no significance difference between industrial type (Manufacturing, Semi-Public Institute, Public Institute) and CTQ type (Product Technology Type CTQ, Process Technology Type CTQ). Following discovery characterize this quality improvement as fixed target type project. As newly developed moving target type of quality innovation performance metric Z-Factor is used for evaluating experimental study, hypothetical analysis suggests that $Z_{st}$ and $P_{pk}$ share different relationship or even show reciprocal relationship. Constraints of the study are relatively small sample size of only 37 projects from past 2 years and conflict on having interview and communication with six sigma quality practitioner for qualitative experimental study. Both moving target type six sigma innovation project and fixed target type improvement project or quality circle enables efficient ways for a better understanding and quality practitioner use by applying quality innovation performance metric. Downside of fixed target type quality performance evaluation metric, $Z_{st}$ and $P_{pk}$, is presented through experimental study. In contrast, advantage of this study is that high throughput requiring product technology, process technology and quantum leap typed innovation effect is evaluated based on precision and accuracy and Z-Factor that enables relative comparison between enterprises is proposed and implemented.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.20 no.3
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• pp.289-295
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• 2009

HRR(High Resolution Range) profiles show one-dimensional radar images including electromagnetic scattering phenomena of a target. Thus, they are not only robust to noise, but also easily obtainable in a real-time. However, in order to construct a training database for the success of radar target identification, a huge amount of HRR profiles are needed because HRR profiles are highly dependent on the relative angle between the radar and the target. In order to alleviate this difficulty, a database construction method based on the scenarios of target's movement is proposed. The proposed method is able to provide a reliable target identification performance even with a small amount of training database.

• Journal of Navigation and Port Research
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• v.39 no.4
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• pp.307-312
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• 2015

ARPA(Automatic Radar Plotting Aid) is a device to calculate CPA(closest point of approach)/TCPA(time of CPA), true course and speed of targets by vector operation of relative courses and speeds. The purpose of this study is to develop target acquisition and tracking technology for ARPA Radar implementation. After examining the previous studies, applicable algorithms and technologies were developed to be combined and basic ARPA functions were developed as a result. As for main research contents, the sequential image processing technology such as combination of grayscale conversion, gaussian smoothing, binary image conversion and labeling was deviced to achieve a proper target acquisition, and the NNS(Nearest Neighbor Search) algorithm was appllied to identify which target came from the previous image and finally Kalman Filter was used to calculate true course and speed of targets as an analysis of target behavior. Also all technologies stated above were implemented as a SW program and installed onboard, and verified the basic ARPA functions to be operable in practical use through onboard test.

• Journal of Information Technology Services
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• v.13 no.1
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• pp.125-134
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• 2014

Device discovery for D2D (device-to-device) communication enables a device to discover other devices in order to initiate communication with them. Devices should perform the discovery phase using a small quantity of radio resource in a short time and be able to reduce the load of the base station. Legacy device discovery schemes have focused on discovering as many target devices as possible. However, it is not appropriate for peer-to-peer D2D communication scenario. Further, synchronization problems are an important issue for discovery signal transmission. This paper proposes a discovery method that one requesting device discovers a specific target for communication. Multiple antenna beamforming is employed for the synchronization between the base station and a target device. The proposal can reduce the load of the base station using the information that it already maintains and improve the reliability of the device discovery because two times of synchronizations using beamforming among the base station and devices can make the exact discovery of a target device with mobility possible.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.7 no.5
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• pp.1166-1179
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• 2013

In wireless sensor network (WSN) environments, environmental noises are generated by, for example, small passing animals, crickets chirping or foliage blowing and will interfere target detection if the noises are higher than the sensor threshold value. For accurate tracking by acoustic WSNs, these environmental noises should be filtered out before initiating track. This paper presents the effect of environmental noises on target tracking and proposes a new algorithm for the noise mitigation in acoustic WSNs. We find that our noise mitigation algorithm works well even for targets with sensing range shorter than the sensor separation as well as with longer sensing ranges. It is also found that noise duration at each sensor affects the performance of the algorithm. A detection algorithm is also presented to account for the Doppler effect which is an important consideration for tracking higher-speed ground targets. For tracking, we use the weighted sensor position centroid to represent the target position measurement and use the Kalman filter (KF) for tracking.