• Title/Summary/Keyword: SlP

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Fluctuations in Phenolic Content and Antioxidant Capacity of Green Vegetable Juices during Refrigerated Storage

  • Kim, Seong Yeong
    • Preventive Nutrition and Food Science
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    • v.20 no.3
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    • pp.169-175
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    • 2015
  • Shinseoncho and kale were made into green vegetable juices by building block [shinsenocho branch (SB), shinsenocho leaf (SL), kale branch (KB), and kale leaf (KL)]. Fluctuations in their phenolic contents and antioxidant capacities were analyzed during refrigerated storage at $4^{\circ}C$ for 28 days. Total polyphenolic contents of leaf parts showed a decreasing tendency after 4 days (SL) or 7 days (KL), whereas branch parts showed fluctuating values during the entire storage period. The 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging capacity was rapidly decreased in SB and in SL at 28 days (P<0.001), whereas KL showed a slightly increasing tendency after 14 days. For the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, SL showed a sharp fall at 28 days (P<0.001), and KL showed a decreasing tendency after 14 days (P<0.001). SB showed a steady decrease during the entire storage period and KB indicated a nearly zero (0.97%) at 28 days. Pearson's coefficients for the correlation between antioxidant capacities measured by the ABTS and DPPH assays, and the total polyphenolic contents were determined. The results showed that the ABTS assay (r=0.934, P<0.001) was more strongly positively correlated with the total phenolic contents than the DPPH assay (r=0.630, P<0.001). In conclusion, when considering all building blocks, green vegetable juices, including kale and shinseoncho may have kept antioxidant capacities for up to 14 days under refrigeration, and the ABTS assay better reflects a positive correlation with the total phenolic contents when compared to the DPPH assay.

Morphological Analysis among Populations of Purpulish Washington Clam, Saxidomus purpuratus on the Korean Waters

  • Kim, Yeong-Hye;Ryu, Dong-Ki;Lee, Dong-Woo;Chang, Dae-Soo;Kim, Jong-Bin;Kim, Seong-Tae;Kwon, Dae-Hyeon
    • The Korean Journal of Malacology
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    • v.22 no.1 s.35
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    • pp.23-26
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    • 2006
  • Morphological differences were studied using the analysis of variance between various partial length and shell length of three populations of Saxidomus purpuratus on the Korean waters. The Relative growth equations, that is, SH-SL, SW-SL, TW-SL of S. purpuratus by sex were estimated. The analysis of variance of four morphological characters proved that each population has no sexual differences (p>0.01). But the three populations are significantly different in morphological characters (p<0.01).

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A Study on the Growth and Spawning of Korean Scallop (Chlamys farreri) around Wando, Korea (한국 완도연안 비단가리비(Chlamys farreri)의 성장과 산란)

  • 강태구;장창익
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.36 no.3
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    • pp.210-221
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    • 2000
  • The population habitat and density of Korean scallops, Chlamys farreri, were investigated to estimate population ecological characteristics from samples randomly collected around Wando from November, 1998 to October, 1999. Age and growth of the Korean scallops were determined from their ring radii. Maturation and spawning were studied using data of ovary maturity stage, gonadosomatic index, and fecundity. Seawater temperature and specific gravity ranged from 7.6 to $25.9^{\circ}C$ and from 1.0188 to 1.0260, respectively. Also dissolved oxygen and pH ranged from 6.48 to 9.50 ppm and from 8.17 to 8.80. Rocky and gravel bottom had a maximum habitat density of $$28.83 inds/100m^2$$ , which accounted for 82.4 % of the overall habitat area. The relationship between shell length (SL, mm) and shell height (SH, mm) of the Korean scallops was fitted : SH=1.021 SL+2.211 $(R^2=0.989)$. The shell length-total weight (TW, g) relationship was $TW=0.0003; SL^{2.837};(R^2=0.980)$. Then von Bertalanffy growth parameters were estimated from a nonlinear regression method, and the values were as follows : $SH_{\infty}=117.4 mm$K=0.61/year,; and; t_0=-0.017 year., The 50 % maturity at age was 0.21 year with the shell height of 18.3 mm, and spawning occurred twice a year, that is, June/July and October. The relationship between fecundity (Fc) and shell length was$Fc=697.03 SL^{2.683}(R^2=0.984)$, and the fecundity-gonad weight (GW, g) relationship was Fc=10,076,090 GW+15,608,781 $(R^2=0.990)$.

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Age and Growth of the Asian Clam Potamocorbula ustulata ustulata (Reeve, 1844) on the West Coast of Korea (한국 서해산 쇄방사늑조개, Potamocorbula ustulata ustulata (Reeve, 1844) 의 연령과 성장)

  • Ryu, Dong-Ki;Kim, Yeong-Hye
    • The Korean Journal of Malacology
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    • v.23 no.1
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    • pp.17-23
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    • 2007
  • Samples of Potamocorbula ustulata ustulata were collected monthly from October 2004 to November 2005 in the Jujin estuary of Gochang, Chollabuk-do, west coast of Korean peninsula. Age of P. ustulata ustulata was determined by the rings on the shell. The relationship between the shell length and the ring diameter in each ring group was expressed as a regression line. Therefore, there is a correspondence in each ring formation. Based on the monthly variation of the marginal index (MI') of the shell, it is assumed that the ring of this species was formed once a year during October to December. The relationship between the shell length (SL) and the shell height (SH; mm) was highly correlated with shell height as the following equation: SH = 0.6438 SL + 0.5642 ($r^2\;=\;0.978$). The shell length (SL) - shell width (SW) relation was also expressed by the following equation: SW = 0.4352 SL - 0.5675 ($r^2\;=\;0.957$). Shell length (SL; mm) and the total weight (TW; g) followed: $TW\;=\;6.999\;{\times}\;10^{-5}\;SL^{3.2542}(r^2\;=\;0.975)$. Growth curves for the shell length and the total weight fitted to the von Bertalanffy's growth curve were expressed respectively as: $$SL_t=30.77[1-e^{-0.4572(t+0.7371)}],\;TW_t=4.87[1-e^{-0.4572(t+0.7371)}]^{3.2542}.$$

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Consideration of the Exercise position for Facilitating Gluteus Medius Maximally in Normal Adults

  • Park, Sung Jin;Park, Ji Won;Kwon, Yonghyun
    • The Journal of Korean Physical Therapy
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    • v.31 no.1
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    • pp.62-66
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    • 2019
  • Purpose: This study examined the most efficient exercise position to activate the gluteus medius (GM) and tensor fascia latae (TFL) in hip abduction in side-lying (HA-SL), clam in side-lying (CL-SL), and sling bridging in side-lying (SB-SL), which are the most representative GM exercises. Methods: Twenty-four healthy male adults aged from 20 to 40 years, whose body mass index was under 25, participated in this experiment. While all participants conducted three different positions with a counterbalanced manner, such as in AB, CL, and BR, activation of the GM and TFL was measured using 8-channel wireless EMG. Exercise was performed for 10 seconds three times in total with a five minute-break session. Results: Significant differences in GM and TFL activation were observed among the three positions (p<0.05). The highest activation of 60.69 was observed in BR followed in order by 46.03 and 12.92 in HA-SL and CL, respectively. TFL activation in HA-SL was 42.01, followed in order by 35.98 and 14.01 in BR and CL, respectively. On the other hand, there was no significant difference in TFL muscle activation between BR and HA-SL. Conclusion: These findings suggest that both BR and HA-SL in GM can be done selectively. CL has remarkably low muscle activation in GM and TFL, which makes it less valuable in GM and TFL exercise. In conclusion, selective BR and HA-SL exercise should be applied to maximally and effectively activate the GM.

Expression of Tunicamycin Resistance in Bacillus subtilsls by Several Transfroming Plasmids

  • Kong, In-Soo;Makari-Yamasaki
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1986.12a
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    • pp.529.2-529
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    • 1986
  • pSp-Si (1.6kbp) was originally found in pediococcus halophilus to be a cryptic multicopy-plasmid. Hoping that the plasmid can also replicate in Bacillus subtilis, protoplast transformation of strain 207-25 (recE) was performed using pSP-Sl onto which was added the marker of tmrB8 (on 4.9 kbp EcoRI fragment ) or tmrB+ (on 0.9 kbp xbaI fragment) gene. Though the tmrB8 gene can expres tunicamycin-resistance at the single copy state, and the tmrB+ gene exerts the resistance only at the multicopy state, we could not confirm the replication of pSP-Sl (tmrB8) or pSP-Sl(tmrB+) in B. subtilis. During the experiment, however, we unexpectedly found that the circularized 0.9 kbp xgaI fragment (tmrB+) itself, which had no replication origin, could transform strain 207-25 to tunicamycin-resistant by protoplast transformation. Southern hybridization analyses with tmrB+ and other probes revealed the integration of the fragment at a single copy state into a position other than the homologous tmrB gene. This recE independent integration of another tmrB+ gene into the chromosome may contribute to the tunicamycinresistance in the transformants.

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Role of Ser-33 and Asp-112 Residues in In vivo Folding of E, coli Tryptophan Synthase $\alpha$ Subunit (트립토판 중합료소 $\alpha$ 소단위체의 대장균내 구조형성과정에서의 Ser-33과 Asp-112 잔기의 역할)

  • 유충배;신혜자;임운기
    • Journal of Life Science
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    • v.6 no.4
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    • pp.304-312
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    • 1996
  • In the present report, a p[ossibility of the interaction fo Ser-33 and Asp-112 residues in folding of tryptophan synthase $\alpha$ subunit was explored by examining the effect of single or double substitution of these residues on folding of $\alpha$ subunit in E. coli. $\alpha$ subunit of which Ser-33 was substituted with Leu (SL33) was accumulated as insoluble aggregate form, when overproduced in E. coli, whereas $\alpha$ subunit of which Asp-112 was replaced by Asn (DN112) or Gly (DG112) was accumulated as soluble form to the similar extent as wild type $\alpha$ subunit was. When these alterations were combined into one protein, the synergistic effect of residues 33 and 112 on the amount of aggregate form was shown. The amount of doubly altered SL33/DG112 $\alpha$ subunit as aggregate form was increased 5-13 fold that of SL33 $\alpha$ subunit, and the amount of SL33/DG112 $\alpha$ subunit as aggregate form was decreased 3-4 fold that of SL33 $\alpha$ subunit. Aggregates are derived from the specific association of partially folded or unassembled subunits in the folding process. Therefore, this result suggests that residues 33 and 112 of $\alpha$ subunit may unteract during the folding of this enzyme in E. coli.

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Product Quality and Shelf-life Effect of Low-fat Functional Sausages Manufactured with Sodium Lactate and Chitosans During Storage at 15℃ (젖산나트륨과 키토산을 첨가한 저지방 기능성 소시지의 15℃에서 저장 중 품질 및 저장성 효과)

  • Chin, Koo-Bok;Kook, Sung-H.;Choi, Soon-H.
    • Journal of Animal Science and Technology
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    • v.47 no.4
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    • pp.655-666
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    • 2005
  • This study was performed to measure physicochemical and textural characteristics, and shelf-life effect of low-fat functional sausages(LFFSs) manufactured with sodium lactate(SL, 3.3%), lac pigment and various molecular weights(MWs) of chitosan (Low=1.5 kDa, Med=30-50 kDa and High=200 kDa) during storage at 15$^{\circ}C$ for 18 days. LFFSs had 73.7-76.0% moisture, lower than 3% fat and 14-15% protein, respectively. pH values were 6.05-6.44 and the control(150 ppm, $NaNO_2$) was the lowest among LFFSs (p<0.05). Increasing storage time decreased pH values, but no differences in pH values were observed up to 6 days of storage (p>0.05). LFFSs containing SL and low MW of chitosan improved water holding capacity (WHC) and different from those with SL and medium-MW chitosan. WHC was decreased with increased storage time and differences of WHC were observed from 18 days of storage. The addition of chitosan reduced both lightness and redness values, as compared to 150 ppm sodium nitrite(SN), and increased storage time decreased yellowness(p<0.05), especially at 12 days of storage. LFFSs with SL and medium-MW chitosan increased most textural properties compared to the control(p<0.05). The addition of SN of 150 ppm in LFFSs retarded microbial growth for E. coli 0157:H7, while those with SL tended to have an antimicrobial effect for Listeria monocytogenes(LM). The growth rate of LM was delayed by addition of various MW of chitosans in LFFSs, especially high MW chitosan, as compared to LFFSs containing SL alone. These results indicated that the functional, textural and antimicrobial effects of LFFSs were improved by addition SL and various MW of chitosan combinations. In addition, 0.05% lac pigment improved the cure color of LFFSs similar to those of 150 ppm SN.

Use of Paper Mill Sludge and Briquet Ash as Root Zone Soil Mixtures for Thrfgrass Culture (잔디상토로서의 제지스럿지와 연탄재 이용에 관한 연구)

  • 구자영;김태일;안주원
    • Asian Journal of Turfgrass Science
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    • v.6 no.1
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    • pp.11-22
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    • 1992
  • To determine the use of waste materials as root zone soil mixtures for turfgrass culture, the effects of paper mill sludge and briquet ash on physical and chemical properties of soil and growth of turfgrasses were examined. Three turfgrass species of zoysiagrass(Zoysia japonicaSteud.). kentycky bluegrass(Poa pratensis L. 'Ram I') and creeping bentgrass(Agrostis panistris Huds 'Persucross') were cultured in 32cm diameter plastic pots containing various soil mixtures. The basic ingredients used for mixtures included sand(SD), field soil(SL), paper mill sludge(PS), sphagnum peat moss(PM) and briquet ash(BA). Seven combinations using these ingreients were mixed in different percentage by volume as follows: SD+SL+PM(80:10.10), SH+SL+PS(80:10:10), SD-PM(80:20), SD+PS (80:20), SD+BA(80:20), SD+BA+PM(60:20:20) and SD+BA+PS(60:20:20). 1. Paper mill sludge showed pH of 6.6, more than 30% of organic matter content, and higher concentrations of total N, P, k, Ca, Mg and CEC. Bulk density, fild moisture capacity and electrical conductivity of soil mixtures were increased by the comimation of 10~20% PS by volume. 2. Briquet ash showed pH of 8.0, and higher levels of P, k, Ca and Mg than those of field soiks. Bulk density, field moisture capacity and hardenss of soil mixtures were increased but vertical water flow rate and electrical conductivity were decreased by the combination of 20% BA by volume. 3. Phytotoxic effects of PS and BA on growth of turfgrasses were not found. Shoot growth of all three species was higher in soil combination of SD+BA+PS than that of SD+SL+PM added with fertilizer. However, root growth was better in soil mixtures combined with PM. Soil mixtureomposed of 60% SD, 20% BA and 20% PS by volume was most effective on growth of all three species. 4. Paper mill sludge resulted in higher N level in the leaf tissue. The contents of heavy metals such as Cd and Ph did not vary significantly among soil mixtures and species. However, the Mn level was 2~3 times higher in plants growh in mixtures containing PM compared with others, and especially it was higher in creeping bentgrass than other species.

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Computation of Irrigation Interval and Amount as affected by Growing Substrate and Soil Depth Planted with Zoysia japonica in Green Roof during a Dry Summer (여름철 무강우 시 들잔디 옥상녹화 식재지반에 따른 관수주기 및 관수량 산정)

  • Ju, Jin-Hee;Bae, Gyu-Tae;Kim, Won-Tae;Yoon, Yong-Han
    • Journal of Environmental Science International
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    • v.21 no.3
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    • pp.289-296
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    • 2012
  • The purpose of this study was to identify the irrigation intervals and the amount of suitable growing substrate needed to achieve the desired shallow-extensive green roof system during a dry summer in Korea. In terms of treatment, three types (SL, $P_6P_2L_2$, $P_4P_4L_2$) with varying soil mixture ratios and two types (15 cm, 25 cm) with varying soil depths were created. The results have been analyzed after measuring growth and soil water contents. The difference of growth by treatment was significant in terms of green coverage, height, leaf width and photosynthesis. In measurement of chlorophyll content, no difference was detected when measured against soil depth. According to the growth measurement of Zoysia japonica with respect to differing soil mixture ratios in the 15 cm-deep treatment, a statistical difference was detected at the 0.05 significance level in photosynthesis. In case of green coverage, height, chlorophyll content and leaf width, no statistical significance was observed. In case of the 25 cm-deep treatment, a statistical significance was observed in height and photosynthesis. In terms of green coverage, chlorophyll content and leaf width, no statistical significance was detected. In comparisons of soil moisture tension and soil water contents, the irrigation interval and amount were 8 days and 14.9 L in the SL (15 cm) treatment, respectively. The irrigation interval showed for 10 days a 1.3-fold increase, and the irrigation amount was 27.4 L 1.8-fold more than SL (25 cm), respectively. For $P_6P_2L_2$ (15 cm) treatment, the irrigation interval and amount were 12 days and 20.7 L, respectively. However, an irrigation interval under $P_6P_2L_2$ (25 cm) was for 15 days 1.3 times longer than $P_6P_2L_2$ (15 cm), and an irrigation amount of 40 L was 1.9 times more than that under $P_6P_2L_2$ (15 cm). In $P_4P_4L_2$ (15 cm) treatment, it was indicated that the irrigation interval was 15 days, and the irrigation amount was 19.2 L. It was not needed to irrigate for 16 days under $P_4P_4L_2$ (25 cm) treatment during the dry summer and the longest no-rain periods. The irrigation interval and amount under $P_4P_4L_2$ were 1.8-fold and 1.3-fold, respectively, more than SL treatment as affected by soil mixture ratio. Comparatively $P_4P_4L_2$ had more 1.3-fold and 0.9-fold in irrigation interval and amount more than $P_6P_2L_2$. Therefore, it can be noted that different soil depth and soil mixture ratios had a significant effect on the irrigation interval and amount.