• KSBB Journal
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• 제31권1호
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• pp.14-19
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• 2016

To investigate the effect of peanut sprout extract on skin care, we measured anti-oxidant activity and whitening action. As a result of measuring DPPH radical scavenging activity to examine independent anti-oxidation of peanut sprout extract, there was strongly scavenging activity. Fluorescent material DCF-DA was used to measure hydrogen peroxide created in RAW 264.7 cells, and all concentration dependently decreased ROS production. As a result of measuring nitric oxide to examine anti-inflammation of peanut sprout extract, there was strongly inhibited nitric oxide production in RAW 264.7 cells. Tyrosinase activation was found to inhibited dose-dependant. Melanin production was also prevented dose-dependant. Therefore, it is expected to be used effectively in development of functional cosmetic materials.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.672-673
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• 2003

• Natural Product Sciences
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• 제17권3호
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• pp.198-201
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• 2011

Tyrosinase is one of the important enzymes in the mammalian melanin synthesis. In the process of melanin synthesis, tyrosine is oxidized to DOPA (3,4-dihydroxyphenylalanine), and DOPA is further oxidized to dopaquinone. Tyrosinase is an enzyme catalyzing this oxidation of tyrosine, so chemicals that inhibit the activity of tyrosinase can be used as skin whitening agents. In this study, we isolated five constituents from the 80% MeOH extract of Morus bombycis cortex by bioactivity-guided fractionation. We performed mushroom tyrosinase inhibition assay. As a result, 7,2',4'-trihydroxyflavanone (1), 2',4',2,4,-tetrahydroxychalcone (2), and oxyresveratrol (3) showed the more potent inhibitory effect compared to kojic acid, a well-known skin whitening agent with antityrosinase effect. Moracinoside M (4) and moracin M-3'-O-${\beta}$-D-glucopyranoside (5) also showed the moderate tyrosinase inhibitory activities.

• Natural Product Sciences
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• 제16권2호
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• pp.133-139
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• 2010

In order to develop a new skin whitening agent, approximately 100 plant extracts were evaluated for their inhibitory activities against melanin biosynthesis in cultured mouse melanocyte melan-a cells. As a result, seven extracts exhibited over 50% inhibition of melanin synthesis compared to control at a concentration of $20\;{\mu}g/ml$. In particular, Aster ageratoides Turcz. var. ageratoides (branch, root, aerial, flower; $IC_{50}$ = 17.3, 6.1, 13.6, $12.9\;{\mu}g/ml$, respectively) and Physalis alkekengi var. francheti (leaf, unripen fructus, aerial; $IC_{50}$ = 6.5, 28.3, $23.9\;{\mu}g/ml$) markedly inhibited melanin synthesis. In addition, tyrosinase activity was monitored by the measurement of dopachrome formation from the oxidation of L-3,4-dihydroxyphenylalanine. Extracts of A. ageratoides Turcz. var. ageratoides (flower) and P. alkekengi var. francheti (leaf) showed the most potent tyrosinase inhibitory activity. These plants might be the potential candidate sources in the development of novel skin-whitening products.

• 한국수산과학회지
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• 제54권3호
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• pp.271-279
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• 2021

Ultrasound-assisted extraction (UAE) conditions, including extraction time, extraction temperature, and ethanol concentration, were optimized to increase the total flavonoid content (TFC), tyrosinase inhibitory activity (TIA), and collagenase inhibitory activity (CIA) of Spirulina platensis through central composite design (CCD). For the optimization of the three dependent variables, a quadratic regression model was derived from 17 experimental sets for the simultaneous maximization of TFC, TIA, and CIA. The predicted optimal UAE conditions were 44.0 min of extraction time, 82.8℃ of extraction temperature, and 96.0% of ethanol concentration. Under these conditions, 0.93 mg QE/g DM of TFC, 81.9% of CIA, and 92.1% of TIA were predicted. The CCD-based UAE optimization enabled an increase in TFC, CIA, and TIA, thereby confirming that the S. platensis extract can be used in the development of a cosmetic material with skin whitening and anti-wrinkle effects.

• 패션비즈니스
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• 제25권2호
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• pp.51-62
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• 2021

This study evaluated the cosmetic effects of a far-infrared hot and cool mask (BBSkinplus) on face skin moisturization, whitening, and wrinkles using a randomized clinical trial. Forty female participants (mean age 47.7 ± 4.6 years) were allocated into the treatment group (BBSkinplus treatment and lotion application twice a day) or the control group (only lotion application twice a day), and moisture, brightness, and wrinkles were measured at 0, 1 and 2 weeks. The average values of each measurement were changed significantly by BBSkinplus treatment compared to the control group. At the 2-week treatment time point, the statistical differences in improvement rates between the two groups were significant at 8.7% vs. 3.9% for moisture (p < 0.01), 0.7% vs. 0.4% for brightness (p < 0.01), and -3.1% vs. 0.0% as Ra and -6.3% vs. 1.5% as R3z for wrinkles (p < 0.05), respectively. No notable complaint was reported regarding any kind of adverse effects such as erythema, itching, or burning. This clinical data support the potential of BBSkinplus as a home beauty device providing moisturizing, whitening, and anti-wrinkle effects on the face.

• Biomolecules & Therapeutics
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• 제23권3호
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• pp.283-289
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• 2015

The half-dried leaves of Stewartia. pseudocamellia were extracted with hot water (SPE) and partitioned with n-hexane (SPEH), dichloromethane (SPED), and ethyl acetate (SPEE) successively. SPE and SPEE showed significant inhibitory effects against melanogenesis and tyrosinase activities. By bioassay-guided isolation, ten phenolic compounds were isolated by column chromatography from SPEE. The whitening effect of the isolated compounds from SPEE were tested for the inhibitory activities against melanogenesis using B16 melanoma cells, in vitro inhibition of tyrosinase, and L-3,4-dihydorxy-indole-2-carboxylic acid (L-DOPA) auto-oxidation assay. A cytotoxic activity assay was done to examine the cellular toxicity in Raw 264.7 macrophage cells. Of the compounds isolated, gallic acid and quercetin revealed significant inhibitory activities against melanogenesis compared to arbutin. In particular, quercetin exhibited similar inhibitory activities against tyrosinase and L-DOPA oxidation without cytotoxicity. These results suggested that SPE could be used as a potential source of natural skin-whitening material in cosmetics as well as in food products.

• 통합자연과학논문집
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• 제5권1호
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• pp.46-52
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• 2012

Mulberry extracts can be incorporated into skin-whitening products. The compound attributed to lighten the skin is arbutin, a form of hydroquinone that inhibits melanin release by suppressing the tyrosinase enzyme. For the cosmetic applications, the physiological effects of mulberry (Morus alba) extracts were investigated. The water soluble fraction of mulberry contains higher amount of protein (16.28~4.47%) in contrast to fat (1.55~1.41%). In addition, the fraction abundantly contains succinic acid (972.4-275.8 mg/g) and phosphoric acid (1,628.4-121.9 mg/g) in different parts of mulberry. The free radical scavenging ability in water soluble fraction was found to display remarkable effects in comparison with methanol and ethyl acetate fraction. The ethyl acetate-soluble of root and leaf showed remarkable tyrosinase inhibition activity by IC 50 (${\mu}g/ml$). The anticancer activity of methanol fraction obtained from mulberry using human cancer cell lines showed growth inhibition effect (270.14 mg/ml in Calu-6 cells, 295.29 mg/ml in HCT-116, and 332.29 mg/ml in MCF-7 cells, respectively). Based on the results, Morus alba extracts include cosmetic ingredients with antioxidizing and whitening properties.

• 디지털융복합연구
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• 제12권4호
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• pp.419-425
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• 2014

본 연구의 목적은 강활과 벌사상자 추출물에 의한 피부 멜라닌 형성 저해와 안정성과 자극성 시험을 통하여 기능성 화장품의 사용 물질 가능성을 평가하고자 하였다. 본 실험에 사용된 추출물은 White-$AK^{TM}$이고 INCI 명칭은 Osthole이다. White-$AK^{TM}$의 주성분은 쿠마린이고 EC50 값은 마우스 멜라노마 B16 세포 시험에서 2.7ppm이였다. White-$AK^{TM}$는 알부틴보다 100배 낮은 농도에서 저해 효과를 보였다. 미백 효과의 주 기작은 티로시나제-관련 유전자 발현을 저해하는 것으로 생각되었다. White-$AK^{TM}$ 5% 용액을 함유한 기본적인 에센스 처방을 피부에 적용 시 피부자극을 경감시켰다. White-$AK^{TM}$은 불투명한 용기에서 4주간 자외선 하에서 100%의 회수율을 보였고 $50^{\circ}C$에서 4주간 97%의 회수율을 보였다. 따라서 피부 미백효과와 자극성 경감효과가 있고 자외선 조건하에서 안정한 White-$AK^{TM}$은 화장품 처방에서 기능성 원료로 사용될 수 있을 것으로 사료된다.

• 대한화장품학회지
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• 제45권3호
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• pp.255-263
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• 2019

이소말톨 글리코시드는 홍삼제조과정에서 인삼이 함유하고 있는 아미노산과 당의 열전환물질로서 홍삼 추출물에 다량 함유된 친수성 퓨란배당체이다. 현재 화장품원료로 자주 사용되고 있는 홍삼추출물의 화장품원료로서의 가치를 재고하기 위하여 홍삼추출물의 주성분인 이소말톨 글리코시드에 대하여 다양한 피부미백활성 실험을 실시 하였다. 우리는 이소말톨 글리코시드에 대한 B16-F10 세포에서의 멜라닌함량 변화, 제브라피쉬배아착색 분석, 버섯티로시나제 저해활성, 피부미백활성 기전결정을 위한 단백질 분석을 실시하였다. 제브라피쉬 멜라닌 함량 분석에서 이소말톨 글리코시드는 처리하지 않은 대조군과 비교하여 50 및 $100{\mu}g/mL$ 처리 후 총 멜라닌 함량을 약 20% 감소 시켰으며 제브라피쉬 속의 타이로시나제 활성을 약 10% 감소시켰다. 이소말톨 글리코시드는 또한 B16-F10 흑색 종에서 멜라닌 함량의 농도의존적인 감소를 보였으며 MITF 인산화 인자인 p-AKT 및 p-ERK의 발현을 증가시키고 MITF의 농도를 감소시켰다. 또한 이소말톨 글리코시드는 세포내 타이로시나제, TRP-1 및 TRP-2 발현을 억제 하였다. 이소말톨 글리코시드의 함량은 인삼 추출물에서 약 3%, 인삼 뿌리에서 약 1%였다. 따라서, 정량적으로 고려할 때 홍삼추출물에 다량 함유되어있는 이소말톨 글리코시드는 홍삼의 미백활성을 나타내는 주요성분 중 하나로 판단된다.