• Journal of Ginseng Research
• /
• 제48권2호
• /
• pp.211-219
• /
• 2024

Background: Recently, plant-derived exosome-like nanoparticles (PDENs) have been isolated, and active research was focusing on understanding their properties and functions. In this study, the characteristics and molecular properties of ginseng root-derived exosome-like nanoparticles (GrDENs) were examined in terms of skin protection. Methods: HPLC-MS protocols were used to analyze the ginsenoside contents in GrDENs. To investigate the beneficial effect of GrDENs on skin, HaCaT cells were pre-treated with GrDENs (0-2 × 10⁹ particles/mL), and followed by UVB irradiation or H₂O₂ exposure. In addition, the antioxidant activity of GrDENs was measured using a fluorescence microscope or flow cytometry. Finally, molecular mechanisms were examined with immunoblotting analysis. Results: GrDENs contained detectable levels of ginsenosides (Re, Rg1, Rb1, Rf, Rg2 (S), Gyp17, Rd, C-Mc1, C-O, and F2). In UVB-irradiated HaCaT cells, GrDENs protected cells from death and reduced ROS production. GrDENs downregulated the mRNA expression of proapoptotic genes, including BAX, caspase-1, -3, -6, -7, and -8 and the ratio of cleaved caspase-8, -9, and -3 in a dose-dependent manner. In addition, GrDENs reduced the mRNA levels of aging-related genes (MMP2 and 3), proinflammatory genes (COX-2 and IL-6), and cellular senescence biomarker p21, possibly by suppressing activator protein-1 signaling. Conclusions: This study demonstrates the protective effects of GrDENs against skin damage caused by UV and oxidative stress, providing new insights into beneficial uses of ginseng. In particular, our results suggest GrDENs as a potential active ingredient in cosmeceuticals to promote skin health.

• 한국의류학회지
• /
• 제30권11호
• /
• pp.1626-1635
• /
• 2006

The purpose of this study was to search for comfortable protective clothing for waste incinerator workers. The experimental protective clothing came in two types: one whose outer side made use of polypropylene film, and the inner side, a non-woven rayon fabric processed with charcoal with a 10% density (CF): and one whose outer side made use of polyolefine and polyamide films, and the inner side, a non-woven polypropylene fabric (NNCF). Experiments were conducted on five healthy adult women whose average age was 21. These experiments were conducted at a climate chamber, in which the temperature and relative humidity were set below $28{\pm}1^{\circ}C\;and\;50{\pm}10%$, respectively. Measured were the rectal temperature, the skin temperature, the sweating rate, the weight loss, the heart rate, the blood pressure, the temperature, and the relative humidity of a microclimate and subjective sensation. These were measured within a period of 60 min, consisting of a 20-min stable period, a 20-min exercise period (walking exercise for 2 miles/hr on a treadmill), and a 20-min recovery period. Through this experiment, the differences between the human body's physiological reactions to CF and NNCF clothing, and the human body's comfort levels when wearing these, were determined.

• 한국의류학회지
• /
• 제30권6호
• /
• pp.981-991
• /
• 2006

The purpose of this study was to determine the practicability of an experimental protective clothing by identifying the human body's physiological responses to it as well as the human body's comfort level when wearing it, particularly with the use of a processed charcoal material. The experimental protective clothing came in two types: one whose outer side made use of polypropylene film, and the inner side, a non-woven rayon fabric; and one whose inner side made use of a non-woven fabric processed with charcoal with a 10% density. Experiments were conducted on five healthy adult women whose average age was 21. These experiments were conducted at a climatic chamber, in which the temperature and relative humidity were set below $28{\pm}1^{\circ}C\;and\;50{\pm}10%$, respectively, and were measured within a period of 60min, consisting of a 20-min rest period, a 20-min exercise period, and a 20-min recovery period. Based on the results of this study, the efficiency of the processed charcoal material was reviewed, and a database requiring the production of more functional and comfortable protective clothing materials was established.

• 대한본초학회지
• /
• 제24권3호
• /
• pp.103-108
• /
• 2009

Objectives : This study was carried out to investigate anti-oxidative effects of Taraxaci Herba (TH) and protective effects on Human HaCaT keratinocyte. Methods : Anti-oxidative effects were measured by estimating the amount of total phenolics and flavonoids. In addition, DPPH free radical scavenging activities were estimated. Protective effects of TH on HaCaT keratinocytes against oxidative stress induced by hydrogen peroxide were also measured. Results : In our results, treatment with TH did not show cytotoxicity on HaCaT keratinocyte beneath the concentration of 200 $\mu$g/ml. 42.64$\pm$1.90 $\mu$g/ml of total phenolics and 28.09$\pm$1.84 $\mu$g/ml of flavonoids was detected from TH ethanol extract. In addition, DPPH free radical scavenging activities of TH were elevated in dose-dependent manner. In addition, The value of half maximal inhibitory concentration (IC$_{50}$) was 165.5 $\mu$g/ml. Finally, TH showed protective effect against cell death of HaCaT cell induced by hydrogen peroxide significantly. Conclusions : In conclusion, these results suggest that TH may have anti-oxidantic action in human skin and also suggest the possibility as cosmetic material.

• 대한한방부인과학회지
• /
• 제22권3호
• /
• pp.36-50
• /
• 2009

Purpose: This study was to determine the protective effects of Dioscoreae Rhizoma on the Mouse Embrio Fibroblast (MEF) cells exposed to the ultraviolet rays(UVA). Methods: The samples were assigned randomly to five groups; control group without any treatments, UVA group exposed only to UVA, DR group exposed only to the Dioscoreae Rhizoma, UVA-DR group exposed to UVA before being treated with the Dioscoreae Rhizoma, and DR-UVA group treated with the Dioscoreae Rhizoma before being exposed to UVA. The survival rate of cells, metabolic rate of cells, transformation of nucleus within cells, alteration of cell cycle, effects on the apoptosis, the change of the amount of protein related to cell cycle were measured in order to determine the cell protective effects of the Dioscoreae Rhizoma on each group. Results: 1. DR-UVA group has more cell protective effects compared to the UVA group in all experiments, indicating that the Dioscoreae Rhizoma protects skin from UVA physically and chemically. 2. UVA-DR group shows more efficiency compared to UVA group in rapid recovery of damaged cell and leading highly damaged cells to apoptosis, preventing the expression of abnormal cells. Conclusions: Dioscoreae Rhizoma has effects of protecting MEF cells from UVA, of recovering cells damaged by UVA, and of prohibiting the expression of abnormal cells.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
• /
• pp.54-54
• /
• 2023

Nypa fruticans Wurmb (N. fruticans) is a plant that belongs to Araceae and N. fruticans is mainly found in tropical mangrove systems. The parts (leaves, stems, and roots) of N. fruticans are traditionally used for asthma, sore throat, and liver disease. N. fruticans contains flavonoids and polyphenols, which are substances that have inhibitory effects on cancer and oxidant. In previous studies, some pharmaceutical effects of N. fruticans on melanogenesis and inflammation have been reported. The present study is conducted to investigate the effect of the ethyl acetate fraction of N. fruticans (ENF) on oxidative DNA damage and UVB-induced DNA damage. DNA damage response (DDR) pathway is important in research on cancer, apoptosis, and so on. DDR pathways are considered a crucial factor affecting the alleviation of cellular damage. ENF could reduce oxidative DNA damage derived from reactive oxygen species by the Fenton reaction. Also, ENF reduced the intensity of intracellular ROS in the live cell image by DCFDA assay. UVB is known to cause skin and cellular damage, then finally contribute to causing the formation of tumors. As for the strategies of reducing DNA damage by UVB, inhibition of p53, H2AX, and Chk2 can be important indexes to protect the human body from DNA damage. As a result of confirming the protective effect of ENF for UVB damage, MMPs significantly decreased, and the expression of apoptosis-related factors tended to decrease. In conclusion, ENF can provide protective effects against double-stranded DNA break (DSB) caused by oxidative DNA damage and UVB-induced DNA damage. These results are considered to be closely related to the protective effect against radicals based on catechin, epicatechin, and isoquercitrin contained in ENF. Based on these results, it is thought that additional mechanism studies for inhibiting cell damage are needed.

• 한국식품영양학회지
• /
• 제30권2호
• /
• pp.211-217
• /
• 2017

본 연구는 국내에서 주로 소비되는 곡류를 사용하여 UVB를 조사한 인체 피부 섬유아세포에서 항산화 효능과 MMP-1 및 수용성 콜라겐의 변화를 측정하여 광노화 억제 효능을 평가하였다. 또한 곡류 추출물의 항산화 활성 및 항산화 성분을 측정하고, 항산화력과 항산화 성분과의 상관성을 비교 분석하였다. 7종의 곡류 추출물(백미, 현미, 보리, 율무, 귀리, 수수, 흑미)은 $30mJ/cm^2$ UVB에 노출된 인체 피부 섬유아세포에서 광노화에 대해 세포 보호효과가 있는 것을 확인하였다. 또한 곡류 추출물의 처리는 UVB 조사에 따른 MMP-1의 생성을 감소시키고, 수용성 콜라겐의 분해를 저해하였으며, ROS의 생성도 억제하였다. 7종의 곡류 메탄올 추출물에 대한 총 폴리페놀 함량을 분석한 결과, 흑미와 수수가 다른 곡류에 비해 높은 항산화 성분을 함유하고 있었으며, DPPH 및 ABTS 라디칼 소거능도 높은 활성을 나타냈다. 곡류 추출물의 항산화 성분과 항산화 활성과의 상관분석 결과, 폴리페놀 함량이 높을수록 DPPH 라디칼 소거능($R^2=0.9936$), ABTS 라디칼 소거능($R^2=0.9456$)이 증가하는 것으로 나타났다. 따라서 곡류 추출물은 항산화 성분을 다량 함유하고 높은 항산화 활성을 지녔으며, UVB 조사에 의한 ROS로부터 보호하여 MMP-1의 생성을 감소시키고, 수용성 콜라겐의 분해를 억제시켜 광노화 보효호과를 나타내는 것을 확인하였다. 본 연구 결과, 곡류 추출믈의 항산화 및 광노화 보호효능 입증으로 곡류 추출물을 항주름 기능성 소재에 활용할 수 있을 것으로 판단된다.

• 대한화장품학회지
• /
• 제42권1호
• /
• pp.87-94
• /
• 2016

피부 손상은 주로 자외선, 열, 담배 등과 같은 환경적 요인으로부터 초래되는데, 이는 활성산소종의 과생성으로 인한 피부노화와 연관이 있는 것으로 알려져 있다. 돌배(Pyrus pyrifolia NAKAI)는 전 세계적으로 많이 소비되는 과일로써 항암, 항산화, 항염증효과가 알려져 있다. 본 연구에서는 돌배나무잎 추출물(Pyrus pyrifolia leaf extract, PPE)의 ultraviolet B (UVB)스트레스에 대한 피부 섬유아세포 보호효과를 검증하였다. Lactate dehydrogenase assay와 DCF-DA를 이용한 정성분석 실험은 PPE가 인간의 섬유아세포에서 UVB 스트레스에 의해 유발된 세포독성 및 과생성된 활성산소종을 농도 의존적으로 억제할 뿐만 아니라, 미토콘드리아 기능저하, 막전위 저하, 그리고 세포사멸과정의 핵심 인자인 caspase-3 활성도 유의하게 억제함을 보여주었다. 결론적으로, PPE는 UVB스트레스에 의해 과생성된 활성산소종을 억제시켰으며, 이로 인해 생기는 피부세포 사멸을 효과적으로 저해함을 확인하였다.

• 대한화장품학회지
• /
• 제42권4호
• /
• pp.343-349
• /
• 2016

제주용암해수는 미네랄과 영양염류가 풍부한 물로 제주만이 보유한 지하수자원이다. 본 연구의 목적은 제주용암해수의 피부 보습효과를 확인하기 위한 것이다. 피부의 건조함을 막고 수분을 유지하기 위해서는 표피층의 장벽기능이 정상적으로 기능하고, 표피층 내 수분의 유지와 이동이 원활히 이루어져야 한다. 제주용암해수를 각질형성세포에 처리한 결과 표피층의 분화과정과 natural moisturizing factor (NMF) 생성과정에 관여하는 유전자인 필라그린과 caspase-14 유전자의 발현양이 증가되는 것을 확인할 수 있었다. 또한 막관통 단백질로 수분의 이동을 조절하는 aquaporin 3 (AQP3) 유전자 발현양과 단백질 발현양도 제주용암해수 처리에 의해 증가하였다. 인공피부를 이용한 실험에서 제주용암해수를 배지에 처리하고 배양한 결과 hyaluronic acid (HA)의 수용체인 CD44의 발현양이 증가하였다. 본 연구를 통해 제주용암해수는 피부 보습과 관련된 인자들의 발현양을 증가시켜 피부의 보습기능에 도움을 주는 것으로 사료되었다.

• 대한화장품학회지
• /
• 제44권3호
• /
• pp.277-284
• /
• 2018

콜라겐 펩타이드(collagen peptide)는 단백질의 가수 분해물로서 주름 완화, 보습력 증대, 탄력 개선 등의 특정 피부 효능을 나타낼 수 있어 화장품 혹은 피부 개선 기능식품으로서 활용되고 있다. ${\gamma}-aminobutyric$ acid (GABA)는 척추 동물의 뇌, 척수에 존재하는 신경전달 물질로서 수면의 질과 양을 개선해 준다고 알려져 있다. 본 연구에서는 콜라겐 펩타이드와 GABA의 복합물이 수면 장애가 있는 여성에게 8주 경구 섭취를 통해서 수면 및 피부 상태를 개선할 수 있는가에 관하여 확인하였다. 복합물(J85091900)은 8주간 연속적으로 섭취 시, 수면장애지수(PSQI)가 유의적으로 감소하였으며, 수면 시간을 7% 증가시켰다. 또한, 피부 거칠기, 눈가 주름 및 피부 수분량(capacitance)을 유의적으로 개선하였다. 이상의 결과에서 콜라겐과 GABA의 복합물은 복합 수면 장애에 따른 피부의 노화 현상으로부터 피부를 보호할 수 있음을 확인하여 먹는 화장품의 핵심 소재로 활용 가능함을 확인하였다.