• Korean Journal of Microbiology
• /
• v.36 no.3
• /
• pp.216-220
• /
• 2000

Eighty-eight strain3 o~methicillin resistant Stopllylococcus awecis were Isolated from pus (64.7%); spuhm (26.2%), blood, fluid, andurine of 83 patients at Dong-A Hospital in P~~san to invesligate theil-coagulase typ- Ing, and multi-drug resistaut ppattems. The presence of niec A gene confe~~ing melhicillin resistance was tested by polymerase chain reaction (PCR) with uwo mec A gene specific primers using purified clromosonlal DNA as templates. DNA fragments of expected size wel-e detected frorn 86 strains, but not from two strains. !i coagulase typmg, the 86 isolates were assigned to 5 coagulase lypes, I, 11, lll. 1V, VI, VII, VIlI, but there was no isolate helong lo type V. The most abundant coagulase type was type TI(50 %), lollowed by type IV Rest ofthe coagulase types were ininor; ranging fmm 4.5 to 12.5 '% Most of the type I1 ~netlucillin resistant Stapl\ulcorneryiococcus nwem (MRSA) strams were isolated from the generd sulzely ward, but major strains of type IV were Isolated from the otorhinolq~ngology of the hospital's outpatient clinic center. All of the 88 st~nins were sensitive to vancomycin and teicoplanin, but 71 (81%) strains showed multi-drug resitant to penicillin, cephalotl~n, eiythroinycin, gentan~ycin, imipenem, clindamycin, ciprofloxacin and ooxacillin. Yo relationship was found between the antibiotic resistance pattems aud the coagulase typing patterns.

• Korean Journal of Breeding Science
• /
• v.42 no.6
• /
• pp.695-698
• /
• 2010

The incidence of Anthracnose causing severe damage to the foliage and fruit in watermelon has increased in some major watermelon producing areas in Korea. To develop anthracnose resistant line, 'AU-Producer' having resistant gene to anthracnose was selected from germplasm and crossed with high quality line '920533'. Following the initial cross, backrossing and disease screening were performed to select resistant lines that produced high yields with excellent quality fruit. As a results of these procedure, a mid maturing watermelon line, 'Hangyeol' with resistance to anthracnose (Colletotrichum orbiculare) race 1 and 3 was developed at the National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA). It has red flesh and commonly produces fruit with clear stripes on skin between 5.3 to 10.1 kg. Average soluble solid contents are ranged from 9.8 to 11.8$^{\circ}$Bx. The yield and quality of 'Hangyeol' is comparable to or better than those harvested from the popular commercial cultivars. The achievement of this experiment could contribute to provide the resistant parents in an anthracnose resistance breeding program in watermelon.

• Journal of the Korean Geotechnical Society
• /
• v.24 no.2
• /
• pp.77-86
• /
• 2008

The disturbance zone and measured values are affected by the size of the penetrometer. The local value may be measured by the smaller penetrometer. An ultra small Micro-Cone penetrometer (5mm in outer diameter) is designed and manufactured to characterize soil properties with minimum disturbance during penetration tests. The tip resistance is measured by using stain gauges attached near the Micro-Cone. In addition, the friction sleeve is adopted to effectively remove the skin friction from the tip resistance. Design concern includes the installation of stain gauges, circuits, penetration systems, penetration rate, sampling rate, operating temperature, and calibration. Application tests show that the clay interface, and the soil layers consisting of clay and sand are clearly detected by the Micro-Cone. Furthermore, the cone tip resistances measured by the Micro-Cone and the miniature cone (16mm in outer diameter) are similar. Note the resolution is much higher in the Micro-Cone. This study shows that the Micro-Cone may effectively detect the soil interface with high resolution, and with minimum disturbance.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2022.09a
• /
• pp.36-36
• /
• 2022

The molecular understanding of resistance and susceptibility of host plants to scab, a most threatful disease to pome fruit production worldwide, is very limited. Comparing resistant line '93-3-98' to susceptible one 'Sweet Skin' at seven time points of 0, 0.5, 1, 2, 3, 4, 8 days post inoculation, RNA-sequencing data derived from infected and mock-inoculated young leaves were analyzed to evaluate the tolerant response and to mine candidate genes of pear to the scab pathogen Venturia nashicola. Analysis of the mapped reads showed that the infection of V. nashicola led to significant differential expression of 17,827 transcripts with more than 3-fold change in the seven pairs of libraries, of which 9,672 (54%) are up- and 8,155(46%) are down-regulated. These included mainly receptor (NB-ARC domains-containing, CC-NBS-LRR, TIR-NBS-LRR, seven transmembrane MLO family protein) and transcription factor (ethylene responsive element binding, WRKY DNA-binding protein) related gene. An arsenal of defense response of highly resistant pear accessions derived from European pear was probably supposed no sooner had V. nashicola infected its host than host genes related to disease suppression like Polyketide cyclase/dehydrase and lipid transport protein, WRKY family transcription factor, lectin protein kinase, cystein-rich RLK, calcium-dependent phospholipid-binding copine protein were greatly boosted and eradicated cascade reaction induced by pathogen within 24 hours. To identify transcripts specifically expressed in response to V. nashicola, RT-PCRs were conducted and compare to the expression patterns of seven cultivars with a range of highly resistant to highly susceptible symptom. A DEG belonging to the PR protein family genes that were higher expressed in response to V. nashicola suggesting extraordinary role in the resistance response were led to the identification. This study provides the first transcriptional profile by RNA-seq of the host plant during scab disease and insights into the response of tolerant pear plants to V. nashicola.

• Horticultural Science & Technology
• /
• v.33 no.3
• /
• pp.443-447
• /
• 2015

Fragaria ${\times}$ ananassa (Duch.) cultivars, 'Wongyo 3 115' a nd 'Wongyo 3 116', we re bre d as a n inte rme diate parent l ine using heterosis of $F_1$ hybrids in octoploid strawberry. These two new cultivars were developed as inbred lines ($S_9$ generation) propagated from both seed and runners/stolons. 'Wongyo 3115', an inbred line derived from the 'Benihoppe' cultivar, has a semi-upright plant shape showing a vigorless growth habit, weaker than the original 'Benihoppe' cultivar; it has early flower bud differentiation and produces 10 flowers per cluster when grown from healthy seed. 'Wongyo 3115' has low yields of conical type fruit with pink colored skin; average fruit weight is approximately 11 g. The important characteristic of 'Wongyo 3115' is its excellent fruit firmness, firmer than the original variety. 'Wongyo 3116', an inbred line derived from the 'Doyonoka' cultivar, has an upright plant shape, vigorless growth habit, weaker than that of the original 'Doyonoka' cultivar; it has early flower bud differentiation and produces 8 flowers per cluster when grown from healthy seed. 'Wongyo 3 116' has oblate type fruits with red skin and an ave rage weight of approximately 12 g. The important characteristics of 'Wongyo 3116' are excellent fruit shape and higher yield than other inbred lines, although it produces small fruits compared to the original cultivar. Both cultivars have excellent taste, high sugar/acid ratio, and good texture. In regards to disease and pest resistance, 'Wongyo 3115' and 'Wongyo 3116' have a tendency to be sensitive to powdery mildew, anthracnose and the two-spotted spider mite.

• Korean Journal of Clinical Pharmacy
• /
• v.22 no.1
• /
• pp.55-64
• /
• 2012

The quinolones are broad-spectrum antibiotics and enhanced antimicrobial activity has extended the use of the quinolones beyond the traditional indications for quinolone antibiotics in the treatment of urinary tract infections. The quinolones are effective in a wider variety of infectious diseases, including skin and respiratory infections. Because of their excellent safety and tolerability, they have become popular alternatives to penicillin and cephalosporin derivatives in the treatment of various infections. A retrospective study was performed to evaluate efficacy and safety of IV quinolones for inpatient use. Total 117 patients who administerd quinolones for longer than 3 continuous days at community hospital from October 1st, 2008 to December 31st, 2008 were reviewed. The criterias for drug evaluation were included the validation of indication, outcome, dosage and side effects. In the results, ciprofloxacin 13 (total 93), levofloxacin 3 (total 59) and moxifloxacin 2 (total 19) cases were not met the criterias based on the culture results. Major indications were pneumonia (ciprofloxacin 16.3%, levofloxacin 67.8%, moxifloxacin 84.2%), urinary tract infection (ciprofloxacin 44.1%), skin infection (ciprofloxacin 7.5%, levofloxacin 20.3%, moxifloxacin 10.5%), intra-abdominal infection (ciprofloxacin 10.8%, moxifloxacin 5.3%), etc.. In the results of quinolone monotherapy, the frequencies were each ciprofloxacin 74.2%, levofloxacin 50.8% and moxifloxacin 47.4%. In the results of dosage validation, the validities were each ciprofloxacin 54.8%, levofloxacin 94.9% and moxifloxacin 100.0%. In the results of duration validation, the validities were each ciprofloxacin 59.1%, levofloxacin 78.0% and moxifloxacin 89.5%. Adverse drug reactions were reported for total 49 cases and those were gastrointestinal tract effects including nausea, vomiting, diarrhea and central nervous system effects including headache, dizziness. In summary, the quinolones appropriately used for hospitalized patients based on this study. A focused approach emphasizing "correct use of quinolones" may reduce development of antimicrobial resistance and maximize class efficacy. Consequently, correct use of antibiotics will contribute to decrease medical expenses for person and community.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.13 no.3
• /
• pp.661-668
• /
• 2018

Biomedical signals using skin resistance have different characteristics according to stress diseases. Biological diagnostic devices for diagnosing stress diseases have been developed by using these characteristics, and devices have been developed so that the signals measured by the skin storage meter can be easily analyzed. Experts in the field will look directly at the output signal to determine the likelihood of any stress disorder. However, it is very difficult for a person to accurately determine whether a person to be measured has a stress disorder by analyzing a bio-signal measured by each person to be measured, and the result of the judgment is very likely to be wrong. In order to solve these problems, we implemented the function of determining the signal of a stress disorder by using the machine learning technique. SVM was used as a classification method in consideration of low computing ability of measurement equipment. Training data and test data were randomly generated for each disease using error range 5 based on 13 diseases. Simulation results showed more than 90% decision accuracy. In the future, if the measurement equipment is actually applied to the patients, we can retrain the classifier with the newly generated data.

• Journal of the Korean Geotechnical Society
• /
• v.34 no.3
• /
• pp.67-75
• /
• 2018

In this study, we developed a micropile equipped with ground fixing wedge device which is able to ensure the bearing capacity early before grouting by expanding the tip and exerting the tip surface friction while compressing and expanding the tip of the micropile during loading. The purpose of this study is to verify the applicability of the developed micropile to the ground with various kinds of strength and to compare its characteristics with those of the simple tip expansion micropile. A new test system including a model soil box which can measure the tip resistance and the tip skin friction separately was devised. The loading test was carried out according to the changes of the ground strength and the tip cross section using the devised test systems. As a result of the test, it was found that the developed micropile increased the tip skin friction due to the wedge horizontal force as the soil strength increased and could be applied more effectively to the ground with the strength not lower than the strength of the weathered rock. In addition, it was found that additional bearing capacity could be obtained due to the tip cross section expansion and the wedge horizontal force exertion even in the ground with the strength below the weathered rock strength.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.45 no.4
• /
• pp.415-422
• /
• 2019

Human epidermal stem cells(ESCs) residing in the basement membrane of the skin have an important role in maintenance of skin homeostasis of epidermal layer. Although, ESCs provide new cells to repair damaged tissue in response to tissue injury, subsets of stem cells remain in the quiescent state protected from differentiation and senescence for prolonged survivals. In this perspective, the stem cell niche, which is specific microenvironment composed of niche cells and an extracellular matrix(ECM), supplies the relevant signal to save stem cells from microenvironmental damages. The expression of stemness marker on the surface of ESCs contributes to the attachment on their ECM of the basement membrane, which lead to growth potential and apoptotic resistance against environmental stimuli. In this study, we observed that UV irradiation, a major factor of environmental stimuli, reduced the expression of α2, β1 and α6 integrin in ESCs. Rhus Semialata M extract(RSE) showed inhibitory effect on the UVB-induced reduction of integrin expression. Furthermore, RSE could upregulate the expression of Col-IV and Laminin, which contribute to the attachment of ESCs. These results indicated that RSE could be a potent ingredient for the protection of ESCs from UV irradiation by increasing the expression of integrin and substrate ECM components at their niche.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.6 no.4
• /
• pp.327-343
• /
• 2004

During tunnel construction, ground failures often occur due to existence of weak zones, such as faults, joints, and cavities, ahead of tunnel face. It is hard to detect effectively weak zones, which can lead underground structure to fail after excavation and before supporting, by using conventional characterization methods. In this study, an enhanced analytical method of predicting weak zones ahead of tunnel face is developed to overcome some problems in the conventional geophysical exploration methods. The analytical method is based on Coulomb's and Gauss' laws with considering the characteristics of electric fields subjected to rock mass. Using the developed method, closed form solutions are obtained to detect a spherical shaped zone and an oriented fault ahead of tunnel face respectively. The analytical results suggest that the presence of weak zones and their sizes, location, and states can be accurately predicted by combining a proper inversion process with resistance measured from several electrodes on the tunnel face. It appears that the skin depth or resistivity in rock mass is affected by the diameter of tunnel face, natural electric potential and noises induced by experimental measurement and spatial distribution of uncertain properties. The developed analytical solution is verified through experimental tests. About 1800 concrete blocks of 5cm by 5cm by 5cm in size are prepared and used to model a joint rock mass around tunnel face. Weak zones are simulated ahead of tunnel face with a material which has relatively higher conductivity than concrete blocks. Experimental results on the model test show a good agreement with analytical results.