• Parasites, Hosts and Diseases
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• 제51권4호
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• pp.413-419
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• 2013

The mainstay therapy against leishmaniasis is still pentavalent antimonial drugs; however, the rate of antimony resistance is increasing in endemic regions such as Iran. Understanding the molecular basis of resistance to antimonials could be helpful to improve treatment strategies. This study aimed to recognize genes involved in antimony resistance of Leishmania tropica field isolates. Sensitive and resistant L. tropica parasites were isolated from anthroponotic cutaneous leishmaniasis patients and drug susceptibility of parasites to meglumine antimoniate (Glucantime$^{(R)}$) was confirmed using in vitro assay. Then, complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) and real-time reverse transcriptase-PCR (RT-PCR) approaches were utilized on mRNAs from resistant and sensitive L. tropica isolates. We identified 2 known genes, ubiquitin implicated in protein degradation and amino acid permease (AAP3) involved in arginine uptake. Also, we identified 1 gene encoding hypothetical protein. Real-time RT-PCR revealed a significant upregulation of ubiquitin (2.54-fold), and AAP3 (2.86-fold) (P<0.05) in resistant isolates compared to sensitive ones. Our results suggest that overexpression of ubiquitin and AAP3 could potentially implicated in natural antimony resistance.

• 대한치과보철학회지
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• 제41권2호
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• pp.207-222
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• 2003

As a material of metal-ceramic prosthesis, nickel as a form of Ni-Cr alloy has been used for many dental prostheses in many cases. However, several problems in use of the alloy have been revealed (ex ; tissue stimulation, skin allergy, hypersensitivity cytotoxicity and carcinogenecity). Little is known about nickel with respect to the relationship between Ni-prosthesis and gaining of Niresistance in oral microorganisms. The present study was undertaken to check whether use of Ni-prosthesis leads to occurrence of Ni-resistant microorganisms. So this study may suggest the possible relationships between the oral microorganisms and nickel-resistance in oral environment. Bacteria were isolated from the gingival crevicular fluid on the patients wearing Ni-Cr prosthesis. The isolated bacteria were tested fir their Ni-resistance in nickel containing media at different concentration from 3mM to 110mM. E. coli HB101 was used as control. The Ni-resistant bacteria were isolated and biochemically identified. The Ni-resistant bacteria were tested several biochemical, molecular-biological tests. Performed tests were : measuring the growth curve, antibiotic test, growth ability test in liquid media, isolation of the chromosome and plasmid, digestion of DNA by restriction enzyme, electrophoresis of chromosome and plasmid DNA, identification of Ni-resistant genes by the DNA hybridization. The results were as follows 1) The bacteria isolated from gingival crevicular fluid on the patients wearing Ni-Cr alloy prosthesis showed nickel-resistance. 2) The isolated microorganisms grew at nickel containing media of high concentrations (60mM-110mM). 3) Based on the biochemical tests, the isolated microorganisms were identified as Enterococcus faecalis(13 cases), Klebsiella pneumoniae(1 case) and Enterobacter gergoviae(1 case). 4) Enterococcus faecalis expressed not only nickel resistance but also the multi-drug resistance to several antibiotics ; chloramphenicol, kanamicin, streptomycin, lincomycin, clindamycin, However, all strain showed the sensitivity against the tetracycline. 5) DNA hybridization result suggests that there is no homology between the previously known gene of nickel resistance in Klebsiella pneumoniae and chromosomal DNA of Enterococcus faecalis.

• 한국생활과학회지
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• 제8권1호
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• pp.151-163
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• 1999

The purpose of this study was to investigate the thermal resistance and the liquid/vapor water transfer characteristics of four waterproof and water vapor permeable coated fabrics with the ground fabric called nylon taffeta. In order to establish the experimental environment, outdoor temperature and humidity in Taegu during the last three years were examined and the experiment was performed at (1) $15^{\circ}C$, 50% R.H., (2) $20^{\circ}C$, 60% R.H., (3) $25^{\circ}C$, 65% R.H., which were the average standards in spring and fall. The test results were as follows ; 1. Among physical parameters, the thinner the thickness was, the higher the water vapor permeability was. But the porosity in thickness was not proportional to water vapor permeability linearly. 2. The thicker the thickness of specimens was and the smaller the bulk density and porosity were, the higher the thermal resistance. And the results also shown that the larger the temperature difference between the environmental temperature and the hot plate was, the more the difference of CLO values was apparent. 3. Since the contact angle of all specimens are above $90^{\circ}$, the all specimens have a good performance in waterproof. The more the specimens surface were rough, the higher the thermal resistance was. 4. According to the result of performing moisture transfer test using the simulating body skin-clothing-environment system, the humidity sensor placed in between the fabric and the environment detected the full saturation in 10 minutes after the experiment had began at $15^{\circ}C$, 50% R.H. and in 15 minutes after the experiment both at $20^{\circ}C$, 60% R.H. and at $25^{\circ}C$, 65% R.H. 5. ${\Delta}$ values of the humidity sensors placed in between the human body and the fabric and in between the fabric and the environment fluctuated repeatedly within the range of $20{\sim}40%$ at $20^{\circ}C$, 60% R.H., and $15{\sim}30%$ at $25^{\circ}C$, 65% R.H.

• 한국지반신소재학회논문집
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• 제8권4호
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• pp.19-25
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• 2009

본 연구에서는 접이식 웨지가 장착된 경량 강관네일의 인발저항 증진효과를 평가하기 위하여 동일 지반조건에서 시험시공을 통한 접이식 웨지가 장착된 네일과 웨지가 장착되지 않은 네일의 현장 인발거동 특성을 평가하였다. 강관네일의 현장 인발거동 특성 평가는 극한인발저항력 ($T_L$), 단위주면 마찰저항($q_s$, $u_{max}$), 인발강성계수($K_{\beta}$) 및 네일의 인장력 분석을 통하여 수행되었으며, 그 결과 접이식 웨지를 장착할 경우 풍화토 지반에서 약 30% 정도의 인발저항 증진효과가 있는 것으로 나타났다.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2004년도 학술대회지
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• pp.71-76
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• 2004

This paper investigate that the effect of the concrete containing mineral admixtures(pozzolanic materials such as fly-ash, ground granulated blast-furnace slag, silica fume and meta kaolin) on the resistance properties to chloride ion invasion. The purposed testing procedure was applied to the concrete added mineral admixtures for $3\sim4$ replacement ratios under W/B ratios ranged from 0.40 to 0.55. Specimens were immersed in $3.6\%$ NaCl solution for 330 days, and penetration depth, water soluble chloride contents and acid soluble chloride contents were measured in 28, 91, 182 and 330 days. Then, diffusion coefficient were calculated using total chloride contents. As a results. the kinds of mineral admixture and replacement ratios had a great effect on the resistance property of the concrete to chloride ion invasion compared with the plain concrete. And the optimal replacement ratios of mineral admixture had a limitation for each admixtures. The amount of acid soluble chloride ions and water soluble chloride ions were varied with the kinds of mineral admixtures and the penetration depth from the concrete skin. Chloride diffusion coefficient of each concretes decreased with the time elapsed. and the diffusion coefficients of the concrete immersed salt water for 330 days had a establishment with the compressive strength measured before immersing.

• 한국지반신소재학회논문집
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• 제15권2호
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• pp.65-75
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• 2016

본 연구에서는 최근 적용사례가 급증하고 있는 가압식 그라우팅을 이용한 쏘일네일링의 현장인발시험 자료를 수집하여 데이터베이스를 구성하였으며, 기존의 도해법을 이용한 극한인발저항력 판정법의 문제점을 보완하기 위하여 비선형회귀분석을 이용하여 극한인발저항력을 판정하는 방법을 제안하였다. 비선형회귀분석에 의해 추정된 하중-변위곡선은 현장인발시험 자료와 매우 높은 상관성을 보였으며, 도해법에 의해 판정된 극한인발하중에 비해 평균 29% 정도 크게 판정되었다. 쏘일네일의 하중-변위곡선이 항복하중 이후에 급격한 변위를 보이는 경우에는 S자 성장곡선 회귀모형이 가장 적합하며, 인발하중과 변위의 증가량이 점진적으로 감소하는 파괴거동을 보이는 하중-변위곡선은 점근적 방법이 가장 적합한 회귀모형으로 평가되었다. 본 연구로부터 제안된 단위극한주면 마찰 저항력은 국내 지반특성과 가압식 그라우팅 공법의 특성이 반영된 것으로 해외 연구결과로부터 제시된 설계도표를 이용하던 문제점을 개선함으로써 독자적인 설계기준을 확보하는데 기여할 수 있을 것으로 기대된다.

• Pediatric Infection and Vaccine
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• 제11권2호
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• pp.202-207
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• 2004

A군 연구균 감염으로 인한 폐렴이나 기관지염등 하기도의 감염은 흔하지 않은 것으로 되어 있다. 그러나 일단 발생할 경우에는 심한 경과와 함께 폐농양이나 심장염을 포함해 다양한 염증성 합병증을 동반하며 빠르게 진행한다. 본 저자들은 A군 연구균 감염에 의한 농양성 폐렴을 항생제와 흉강 삽관술로 치료하였고, 연속적으로 발현된 심외막염에 의한 심막삼출을 이뇨제로 치료하여 증상호전을 보인 여자 환아 1례를 경험하였기에 보고하는 바이다.

• 대한약침학회지
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• 제27권2호
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• pp.91-100
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• 2024

Objectives: Candida albicans is an opportunistic pathogen that occurs as harmless commensals in the intestine, urogenital tract, and skin. It has been influenced by a variety of host conditions and has now evolved as a resistant strain. The aim of this study was thus detect the fluconazole resistant C. albicans from the root caries specimens and to computationally evaluate the interactions of an opaque-phase ABC transporter protein with the Psidium guajava bio-active compounds. Methods: 20 carious scrapings were collected from patients with root caries and processed for the isolation of C. albicans and was screened for fluconazole resistance. Genomic DNA was extracted and molecular characterization of Cdrp1 and Cdrp2 was done by PCR amplification. P. guajava methanolic extract was checked for the antifungal efficacy against the resistant strain of C. albicans. Further in-silico docking involves retrieval of ABC transporter protein and ligand optimization, molinspiration assessment on drug likeness, docking simulations and visualizations. Results: 65% of the samples showed the presence of C.albicans and 2 strains were fluconazole resistant. Crude methanolic extract of P. guajava was found to be promising against the fluconazole resistant strains of C. albicans. In-silico docking analysis showed that Myricetin was a promising candidate with a high docking score and other drug ligand interaction scores. Conclusion: The current study emphasizes that bioactive compounds from Psidium guajava to be a promising candidate for treating candidiasis in fluconazole resistant strains of C. albicans However, further in-vivo studies have to be implemented for the experimental validation of the same in improving the oral health and hygiene.

• 한국의류학회지
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• 제15권2호
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• pp.77-87
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• 1991

The purpose of this study were to investigate the bed climate and the physiological responses in sleep on the traditional Korean floor heating system called Ondol. Ondol has been the most widely used heating system in Korea, yet there has been a no systematic studies examined its environmental effects on human body. Experimental room was constructed to match the typical thermal environment of Ondol. (floor surface temperature; $32\~33^{\circ}C$, air temperature; $22.5{\pm}1.0^{\circ}C$, relative humidity; $64.0{\pm}4.0\%$RH, air velocity; 0.25 m/sec.) Three different combinations of bedclothes were chosen for the experiment based on the study results showing that they were the most widely used types in Korea. Type 1 was the combination of a cotton-padded mattress with a cotton-padded Korean style blanket. Type 2 was a cotton-padded mattress with a cotton-guilted Korean style blanket. Type 3 was a cotton-padded matless with a polyacryl blanket. Thermal resistance of each of these combina-tions in the bedclothes was measured using thermal manikin. Two adult female was chosen for the seven hour sleeping experiment which was known to be the average sleeping hours of Korean adult female. The bed climate was measured with the temperature under the mattress, the surface temper-ature of the mattress, and the air temperature and the relative humidity of the space between the mattress and blanket. The skin temperature, rectal temperature of the subjects and the bed climate were measured eight times, one hour before the experiment and every hour during the experiment. The weight loss and the subjective sensation were measured for the each subjects before and after the experiment. The procedure was repeated twice with two subjects and three types of bedclothes, yielding twelve combinations of results. The results were as follows; 1. With the surface temperature of $32\~33^{\circ}C$ of Ondol, air temperature was $22.5{\pm}1.0^{\circ}C$ with $64.0{\pm}4.0\%$ RH. The bed climates were $39.2{\pm}40.8^{\circ}C$ under the mattress, $35.3\~36.2^{\circ}C$ on the mattress, and $26.9\~32.0^{\circ}C$ with $56.0\~71.3\%$ RH between the mattress and the blanket. 2. Mean skim temperature during sleep was 34.2"C with local skin temperature of $34.0\~35.5^{\circ}C$. The skin temperatures of abdomen, thigh, foot were higher than the other parts of the body. 3. The skin temperature of chest, thigh, leg and back varied significantly according to the combinations of bedclothes. With the cotton-padded blanket, the skin temperature was the highest, while with the cotton-guilted blanket showed lowest. 4. Examining the relationship between the mean skin temperature and the local skin temper-ature, the chest temperature showed the highest correlation with the former. Therefore, the chest temperature can be recommended to represent the skin temperature in measuring the bed climate. 5. The subjective bed climates were $39.0\~40.4^{\circ}C$ under the mattress, $35.2\~35.9^{\circ}C$ on the mattress, $29.8\~31.6^{\circ}C$ with $56.8\~68.4\%$ RH between the mattress and blanket. In sum, from this experiment we not only obtained the reliable value of bed climates on Ondol, but also showed that the bed climates and the physiological responses were affected differently according to the materials of bed clothes.

• 한국식품과학회지
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• 제48권3호
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• pp.241-246
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• 2016

S. aureus, S. epidermidis, P. aeruginosa, P. acnes와 C. albicans는 사람의 피부에서 발생하는 질병과 밀접한 관련을 가지는 대표적인 병원성 미생물로 알려져 있다. 본 연구에서는 해조류 중에서도 항균 활성에 대한 연구가 미미한 모자반을 대상으로 피부 병원균에 대한 항균 효과를 조사하였다. 국내의 연해에 자생하는 7종의 모자반 추출물 중에서 disc diffusion assay와 MIC assay를 통해 가장 뛰어난 항균 효과를 나타낸 톱니모자반을 후속 연구를 위한 후보 물질로 선정하고 연구를 진행하였다. 톱니모자반 추출물의 유기용매 분획층 중에서, 노말-헥세인 분획층이 S. aureus, S. epidermidis, P. aeruginosa, P. acnes 및 C. albicans에 대한 MIC값이 $32-256{\mu}g/mL$로 가장 뛰어난 항균 활성을 나타내었다. 이에 피부 병원균들에 대한 치료제로 사용되고 있지만 내성균의 출현으로 효능이 거의 없는 항생제들인 테트라사이클린, 에리트로마이신, 린코마이신과 플루코나졸과 항균 활성이 뛰어난 것으로 나타난 톱니모자반의 노말-헥세인 분획층과의 병용 사용에 의한 항균 시너지 효과를 조사하였다. 그 결과, 톱니모자반 노말-헥세인 분획층과 이들 항생제와의 병용 사용에 의해 피부 병원균에 대한 항생제와 톱니모자반 노말-헥세인 분획층의 MIC값이 4-32배 감소되었고, 톱니모자반 노말-헥세인 분획층과 이들 항생제와의 병용 시 median FIC값이 0.26-0.55로 항균 시너지 효과를 나타내었다. 즉, 항생제와 톱니모자반 노말-헥세인 분획층과의 병용 사용은 피부 병원균에 대한 이들 항생제의 감수성을 회복시키는데 크게 기여하는 것으로 나타났다.